Abstract
Benzoxazolin-2-(3H)-one (BOA) has been tested in many plants species, but not in soybean (Glycine max). Thus, a hydroponic experiment was conducted to assess the effects of BOA on soybean photosynthesis. BOA reduced net photosynthetic rate, stomatal conductance, and effective quantum yield of PSII photochemistry without affecting intercellular CO2 concentration or maximal quantum yield of PSII photochemistry. Results revealed that the reduced stomatal conductance restricted entry of CO2 into substomatal spaces, thus limiting CO2 assimilation. No change found in intercellular CO2 concentration and reduced effective quantum yield of PSII photochemistry revealed that CO2 was not efficiently consumed by the plants. Our data indicated that the effects of BOA on soybean photosynthesis occurred due to the reduced stomatal conductance and decreased efficiency of carbon assimilation. The accumulation of BOA in soybean leaves reinforced these findings.
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Abbreviations
- BOA:
-
benzoxazolin-2-(3H)-one
- Chl:
-
chlorophyll
- C i :
-
intercellular CO2 concentration
- DAC:
-
days of cultivation
- E :
-
transpiration rate
- F0 :
-
minimal fluorescence yield of the light-adapted state
- Fm :
-
maximal fluorescence yield of the dark-adapted state
- Fv :
-
variable fluorescence
- Fv/Fm :
-
maximal quantum yield of PSII photochemistry
- g s :
-
stomatal conductance
- P N :
-
net photosynthetic rate
- ROS:
-
reactive oxygen species
- ΦPSII :
-
effective quantum yield of PSII photochemistry
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Acknowledgments: This work was supported by grants from The Brazilian Council for Scientific and Technological Development - CNPq (no. 470705/2011-6). M.L.L. Ferrarese and O. Ferrarese-Filho are research fellows of CNPq. A.V. Parizotto is the recipient of a CAPES fellowship. The authors are grateful to A.M.D. Ramos for technical assistance.
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Parizotto, A.V., Marchiosi, R., Bubna, G.A. et al. Benzoxazolin-2-(3H)-one reduces photosynthetic activity and chlorophyll fluorescence in soybean. Photosynthetica 55, 386–390 (2017). https://doi.org/10.1007/s11099-016-0656-1
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DOI: https://doi.org/10.1007/s11099-016-0656-1