Abstract
The ameliorative role of 28-homobrassinolide under chilling stress in various growth, photosynthesis, enzymes and biochemical parameters of cucumber (Cucumis sativus L.) were investigated. Cucumber seedlings were sprayed with 0 (control), 10−8, or 10−6 M of 28-homobrassinolide at the 30-day stage. 48 h after treatment plants were exposed for 18 h to chilling temperature (10/8°C, 5/3°C). The most evident effect of chilling stress was the marked reduction in plant growth, chlorophyll (Chl) content, and net photosynthetic rate, efficiency of photosystem II and activities of nitrate reductase and carbonic anhydrase. Moreover, the activities of antioxidant enzymes; catalase (E.C. 1.11.1.6), peroxidase (E.C.1.11.1.7), superoxide dismutase (E.C. 1.15.1.1) along with the proline content in leaves of the cucumber seedlings increased in proportion to chilling temperature. The stressed seedlings of cucumber pretreated with 28-homobrassinolide maintained a higher value of antioxidant enzymes and proline content over the control suggesting the protective mechanism against the ill-effect caused by chilling stress might be operative through an improved antioxidant system. Furthermore, the protective role of 28-homobrassinolide was reflected in improved growth, water relations, photosynthesis and maximum quantum yield of photosystem II both in the presence and absence of chilling stress.
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Abbreviations
- AOS:
-
active oxygen species
- BRs:
-
brassinosteroids
- C i :
-
internal carbon dioxide concentration
- CA:
-
carbonic anhydrase
- CAT:
-
catalase
- CO2 :
-
carbon dioxide
- CS1:
-
chilling stress 1
- CS2:
-
chilling stress 2
- DAS:
-
days after sowing
- DDW:
-
double distilled water
- DM:
-
dry mass
- E :
-
transpiration rate
- EBR:
-
epi-brassinolide
- FM:
-
fresh mass
- g s :
-
stomatal conductance
- HBL:
-
28-homobrassinolide
- LSD:
-
least significant difference
- NR:
-
nitrate reductase
- P N :
-
net photosynthetic rate
- POX:
-
peroxidase
- PPFD:
-
photosynthetic photon flux density
- PSII:
-
photosystem II
- RWC:
-
relative water content
- SOD:
-
superoxide dismutase
- TM:
-
turgor mass
- UV:
-
ultraviolet
- WUE:
-
water-use efficiency
- Ψw :
-
leaf water potential
References
Alam, M.M., Hayat, S., Ali, B., Ahmad, A.: Effect of 28 homobrassinolide on nickel induced changes in Brassica juncea. — Photosynthetica 45: 139–142, 2007.
Ali, B., Hassan, S.A., Hayat, S., Hayat, Q., Yadav, S., Fariduddin, Q., Ahmad, A.: A role for brassinosteroids in the amelioration of aluminium stress through antioxidant system in mung bean (Vigna radiata L. Wilczek). — Environ. Exp. Bot. 62: 153–159, 2008.
Ali, B., Hayat, S., Fariduddin, Q., Ahmad. A.: 24-Epibrassinolide protects against the stress generated by salinity and nickel in Brassica juncea. — Chemosphere 72: 1387–1392, 2008.
Ali, B., Hayat, S., Ahmad, A.: 28-Homobrassinolide ameliorates the salt stress in chickpea (Cicer arietinum L.). — Environ. Exp. Bot. 59: 217–223, 2007.
Ali, M.B., Hahn, E.J., Paek, K.Y.: Effects of temperature on oxidative stress defense systems, lipid peroxidation and lipoxygenase activity in Phalaenopsis. — Plant Physiol. Biochem. 43: 213–223, 2005.
Allen, D.J., Ort, D.R.: Impact of chilling temperatures on photosynthesis in warm-climate plants. — Trends Plant Sci. 6: 36–42, 2001.
Aro, E.-M., Virgin, I., Andersson, B.: Photoinhibition of photosystem II. Inactivation, protein damage and turnover. — Biochim. Biophys. Acta 1143: 113–134, 1993.
Bajguz, A.: Effect of brassinosteroids on nucleic acid and protein content in cultured cells of Chlorella vulgaris. — Plant Physiol. Biochem. 38: 209–215, 2000.
Bajguz, A., Hayat, S.: Effects of brassinosteroids on the plant responses to environmental stresses. — Plant Physiol. Biochem. 47: 1–8, 2009.
Bates, L.S., Waldren, R.P., Teare, I.D.: Rapid determination of free proline for water stress studies.— Plant Soil 39: 205–207, 1973.
Beauchamp, L.D., Fridovich, I.: Superoxide dismutase improved assays and assay applicable to acrylamide gels. — Ann. Biochem. 44: 216–287, 1971.
Berry, J., Björkman, O.: Photosynthetic response and adaptation to temperature in higher plants. — Annu. Rev. Plant. Physiol. 31: 491–543, 1980.
Björkmann, O., Demmig, B.: Photon yield of O2 evolution and chlorophyll fluorescence characterstics at 77 K among vascular plants of different origins. — Planta 170: 489–504, 1987.
Cao, S., Xu, Q., Cao, Y., Qian, K., An, K., Zhu, Y., Hu, B.Z., Zhao, H.F., Kuai, B.K.: Loss-of-function mutations in DET2 gene lead to an enhanced resistance to oxidative stress in Arabidopsis. — Physiol. Plant. 123: 57–66, 2005.
Chance, B., Maehly, A.C.: Assay of catalase and peroxidases. — Meth. Enzymol. 2: 764–775, 1955.
Clouse, S.D., Sasse, J.M.: Brassinosteroids: Essential regulators of plant growth and development. — Annu. Rev. Plant Physiol. Plant Molecular Biol. 49: 427–451, 1998.
Crafts-Brandner, S.J., Salvucci, M.E.: Sensitivity of photosynthesis in a C4 plant maize to heat stress. — Plant Physiol. 129: 1773–1780, 2002.
Dwivedi, R.S., Randhawa, N.S.: Evolution of a rapid test for the hidden hunger of zinc in plants — Plant Soil 40: 445–451, 1974.
Fariduddin, Q., Ahmad, A., Hayat, S.: Responses of Vigna radiata to foliar application of 28-homobrassinolide and kinetin. — Biol. Plant. 48: 465–468, 2004.
Fariduddin, Q., Yusuf, M., Hayat, S., Ahmad A.: Effects of 28-homobrassinolide on antioxidant capacity and photosynthesis in Brassica juncea plants exposed to different levels of copper. — Environ. Exp. Bot. 66: 418–424, 2009a.
Fariduddin, Q., Khanam, S., Hasan, S.A., Ali, B., Hayat, S.A., Ahmad, A.: Effect of 28-homobrassinolide on the drought stress-induced changes in photosynthesis and antioxidant system of Brassica juncea L. — Acta. Physiol. Plant. 31: 889–897, 2009b.
Gomez, K.A., Gomez, A.A.: Statistical Procedures for Agricultural Research. — John Wileys & Sons, New York 1984.
Grove, M.D., Spencer, G.F., Rohwedder, W.K., Mandava, N.B., Worley, J.F., Warthen, J.D., Jr., Steffens, G.L., Flippen-Anderson, J.L., Cook, J.C., Jr.: Brassinolide a plant growth promoting steroid isolated from Brassica napus pollen. — Nature 281: 216–217, 1979.
Hasan, S.A., Hayat, S., Ali, B., Ahmad, A.: 28-homobrassinolide protects chickpea (Cicer arietinum) from cadmium toxicity by stimulating antioxidants. — Environ. Pollut. 151: 60–66, 2008.
Hayat, S., Ali, B., Hasan, S.A., Ahmad, A.: Brassinosteroid enhanced the level of antioxidants under cadmium stress in Brassica juncea. — Environ. Exp. Bot. 60: 33–41, 2007.
Hewitt, E.J.: Sand and Water Culture Methods used in the Study of Plant Nutrition. — Commonwealth Agricultural Bureaux, Farnham Royal, Kent 1966.
Hopkins, W.J.: Introduction to Plant Physiology. — John Wiley & Sons, New York 1995.
Janeckzo, A., Gullner, G., Skoczowski, A., Dubert, F., Barna, B.: Effects of brassinosteroid infilteration prior to cold treatment on ion leakage and pigment contents in rape leaves. — Biol. Plant. 51: 355–358, 2007.
Jaworski, E.G.: Nitrate reductase assay in intact plant tissues. — Biochem.Biophy. Res. Commun. 43: 1274–1279, 1971.
Kagale, S., Divi, U.K., Kronchko, J.E., Keller, W.A., Krishna, P.: Brassinosteroid conifers tolerance in Arabidopsis thaliana and Brassica napus to a range of abiotic stresses. — Planta 225: 353–364, 2007.
Kalinich, J.F., Mandava, N.B., Todhunter, and J.A.: Relationship of nucleic acid metabolism on brassinolide-induced responses in beans. — J. Plant Physiol. 120: 207–214, 1985.
Katsumi, M.: Physiological modes of brassinolide action in ccumber hypocotyls growth. — In: Cutler, H.G., Yokota, T, Adam, G. (ed.): Brassinosteroids: Chemistry, Bioactivity and Applications. American Chemical Society Symposium Series 474. Pp. 246–254, American Chemical Society, Washington, 1991.
Kishore, P.B.K., Sangam, S., Amrutha, R.N., Laxmi, P.S., Naidu, K.R., Rao, K.R.S.S., Rao, S., Reddy, K.J., Theriappan, P., Sreenivasulu, N.: Regulation of proline biosynthesis degradation uptake and transport in higher plants: Its implications in plant growth and abiotic stress tolerance. — Current Science 88: 424–438, 2005.
Kratsch, H.A., Wise, R.R.: The ultrastructure of chilling stress. — Plant Cell Environ. 23: 337–350, 2000.
Krishna, P.: Brassinosteroid-mediated stress responses. — J. Plant Growth Regul. 22: 289–297, 2003.
Lee, H.D., Lee, B.C.: Chilling stress induced changes of antioxidant enzymes in the leaves of cucumber: in gel enzyme activity assays. — Plant Science 159: 75–85, 2000.
Mai, Y.Y., Lin, J.M., Zeng, X.L., Pan, R.J.: Effect of homobrassinolide on the activity of nitrate reductase in rice seedling. — Plant Physiol. Commun. 2: 50–52, 1989.
Morales, D., Rodríguez, D., Dell’Amico, J., Nicolás, E., Torrecillas, A., Sánchez Blanco, M.J.: High temperature pre conditioning and thermal shock imposition affect water relations gas exchange and root hydraulic conductivity in tomato. — Biol. Plant. 47: 203–208, 2003.
Mussig, C., Fischer, S., Altmann, T.: Brassinosteroid regulated gene expression. — Plant Physiol. 129: 1241–1251, 2002.
Oidaira, H., Sano, S.; Koshiba, T.; Ushimaru, T.: Enhancement of antioxidative enzyme activities in chilled rice seedlings. — J. Plant Physiol. 156: 811–813, 2000.
Ozdemir, F., Bor, M., Demiral, T., Turkan, I.: Effects of 24-epibrassinolide on seed germination, seedling growth, lipid peroxidation, proline content and antioxidant system of rice (Oryza sativa L.) under salinity stress. — Plant Growth Regul. 41: 1–9, 2004.
Sairam, R.K.: Effects of homobrassinolide application on plant metabolism and grain yield under irrigated and moisture stress conditions of two wheat varieties. — Plant Growth Regul. 14: 173-181, 1994.
Sairam, R.K., Tyagi, A.: Physiology and molecular biology of salinity stress tolerance in plants. — Current Sci. 86: 407–421, 2004.
Saltveit, M.E., Morris, L.L.: Overview on chilling injury of horticultural crops. — In: Wang, C.Y. (ed.): Chilling Injury of Horticultural Crops. Pp 3–15. CRC Press, Boca Raton 1990.
Salveit, M.E.: Chilling injury is reduced in cucumber and rice seedlings in tomato pericarp discs by heat-shocks applied after chilling. — Postharvest Biol. Tech. 21: 169–177, 2001.
Salvucci, M.E., Crafts-Brander, S.J.: Relationship between the heat tolerance of photosynthesis and the thermal stability of Rubisco activase in plants from contrasting thermal environments. — Plant Physiol. 134: 1460–1470, 2004.
Sasse, J.M.: Physiological actions of brassinosteroids: An update. — J. Plant Growth Regul. 22: 276–288, 2003.
Solomonson, L.P., Barber, M.J.: Assimilatory nitrate reductase functional properties and regulation. — Annu. Rev. Plant Physiol. Mol. Biol. 41: 225–25, 1990.
Sonoike, K.: The different roles of chilling temperature in the photoinhibition of photosystem I and photosystem II. — J. Photochem. Photobiol. 48: 136–141, 1999.
Sutka, J., Galiba, G.: Abiotic Stresses: Cold Stress. — Agr. Res. Inst. Hung. Acad. Sci., Martonvasar 2003.
Taiz, L., Zeiger.: Plant Physiology. 4th Ed. — Sinauer Assoc. Publ., Sunderland 2006.
Tikhomirova, E.V.: Changes in nitrogen metabolism in millet and elevated temperatures. — Field Crops Res. 11: 259–264, 1995.
van Staden, L., Jagr, A.K.: Effects of plant growth regulators on the antioxidant system in seedlings of two maize cultivars subjected to water stress. — Plant Growth Regul. 25: 81–87, 1998.
Vardhini, B.V., Rao, S.S.R.: Amelioration of osmotic stress by brassinosteroids on seed germination and seedling growth of three varieties of sorghum. — Plant Growth Regul. 41: 25–31, 2003.
Walker, M.A., McKersie, B.D.: Role of the ascorbateglutathione antioxidant system in chilling resistance of tomato. — J. Plant Physiol. 141: 234–239, 1993.
Wang, C.Y.: Physiological and biochemical responses of plants to chilling stress. — Hortsci. 17: 173–186, 1982.
Wilen, R.W., Sacco, M., Gusta, L.V., Krishna, P.: Effects of 24-epibrassinolide on freezing and thermotolerance of bomegrass (Bromus inermis) cell cultures. — Physiol. Plant. 95: 195–202, 1995.
Wise, R.R., Naylor, A.W.: Chilling-enhanced photo-oxidation. The peroxidative destruction of lipids during chilling injury to photosynthesis and ultrastructure. — Plant Physiol. 83: 272–277, 1987.
Yang, M.T., Chen, S.L., Lin, C.Y., Chen, Y.M.: Chilling stress suppresses chloroplast development and nuclear gene expression in leaves of mung bean seedlings. — Planta 221: 374–385, 2005.
Yu, J.Q., Huang, L.F., Hu, W.H., Zhou, Y.H., Mao, W.H., Ye, S.F., Noques, S.: A role of brassinosteroids in the regulation of photosynthesis in Cucumis sativus. — J. Exp. Bot. 55: 1135–1143, 2004.
Yu, J.Q., Zhou, Y.H., Huang, L.F., Allen D.J.: Chill induced inhibition of photosynthesis: Genotype variation within Cucumis sativus. — Plant Cell Physiol. 43: 1182–1188, 2002.
Zhou, Y.H., Huang, L.F., Zhang, Y.L., Shi, K., Yu, J.Q., Nogués, S.: Chill induced decrease in capacity of RuBP carboxylation and associated H2O2 accumulation in cucumber leaves are alleviated by grafting onto fig leaf gourd. — Ann. Bot. 100: 839–848, 2007.
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Financial assistance rendered by Department of Science and Technology, New Delhi, India is gratefully acknowledged by Q. Fariduddin
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Fariduddin, Q., Yusuf, M., Chalkoo, S. et al. 28-homobrassinolide improves growth and photosynthesis in Cucumis sativus L. through an enhanced antioxidant system in the presence of chilling stress. Photosynthetica 49, 55–64 (2011). https://doi.org/10.1007/s11099-011-0022-2
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DOI: https://doi.org/10.1007/s11099-011-0022-2