The aim of the present work was to use immunocytochemical methods (detection of Iba-1 protein) to study the morphological characteristics of hippocampal microgliocytes in rats (n = 15) and describe their cell types in the situation of responses to single episodes of total cerebral ischemia lasting 12 min. The results obtained here provided evidence for the existence of several morphological types of microgliocytes in intact hippocampus and the appearance of new forms in responses to ischemia-induced neuron damage. These changes in the overall hippocampal microgliocyte population were associated with functional activation. During the postischemic period (3, 7, and 14 days), microgliocyte processes were characterized by the appearance of multiple microprocesses, pointing to increased interactions between these and the surrounding cellular elements, which may be an indication of a process eliminating damaged synaptic structures from the neuropil.
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Translated from Morfologiya, Vol. 142, No. 5, pp. 30–33, September–October, 2012.
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Korzhevskii, D.E., Lentsman, M.V., Kirik, O.V. et al. Morphological Types of Activated Microglial Cells in the Hippocampus Present after Transient Total Cerebral Ischemia. Neurosci Behav Physi 43, 861–864 (2013). https://doi.org/10.1007/s11055-013-9818-9
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DOI: https://doi.org/10.1007/s11055-013-9818-9