Abstract
The present study aimed to analyze the growth kinetics and morphogenesis of toxigenic Fusarium verticillioides strains. Growth curves based on mycelial dry weight measured after 24, 48, and 96 h and every 4 days for a period of 60 days were obtained for each strain. The morphogenesis of this fungus was studied during its developmental stages through the fluorescent method Fluorecein Diacetate-FDA and Ethidium Bromide-EB. The growth curves of the strains usually followed a homogeneous pattern comparable to the ideal growth curve. Using the fluorescence method, non-viable cells showed a light bright red coloration and viable cells presented green fluorescence with three fluorescent patterns. The present results showed that the morphogenesis of F. verticillioides is an asynchronous process characterized by the presence of a wide variety of fungal forms until 50 days of culture. The method is very useful to demonstrate the F. verticillioides growth stages as well as the perfect differentiation between viable and non-viable cell.
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Kerényi Z, Zeller KA, Hornok L, Leslie JF. Molecular standardization of mating type terminology in the Gibberella fujikuroi species complex. Appl Environ Microbiol 1999;65:4071–6.
Lacaz CS, Porto E, Martins JEC, Vaccari EM, Melo NT. Tratado de Micologia Médica. São Paulo: Sarvier; 2002. 1104 p.
Bacon CW, Nelson PE. Fumonisin production in corn by toxigenic strains of Fusarium moniliforme and Fusarium proliferatum. J Food Prot 1994;57:514–21.
Pitt JI, Hocking AD. Fungi and food spoilage. 2nd ed. London: Blackie Academic & Professional; 1997. 593 p.
Council for Agricultural Science and Technology (CAST). Mycotoxins: risks in plant, animal, and human systems. Task Force Report No. 139, USA; 2003. 199 p.
Sutton DA, Fothergill AW, Rinaldi MG. Guide to clinically significant fungi. 1st ed. Baltimoe MD: Willians and Willians; 1998.
Frisvad JC, Samson RA. Filamentous fungi in foods and feeds; ecology, spoilage and mycotoxins productions. In: Arora DKKG, Mukerji KG, Marth EH, editors. Handbook of applied mycology: foods and feeds, vol 3. New York: Marcel Dekker; 1991. p. 621.
Rotman B, Papermaster BW. Membrane properties of living mammalian cells as studied by enzymatic hydrolases of fluorogenic esters. Proc Nat Acad Sci USA 1966;55:134–44.
Edidin M. A rapid quantitative assay for cell damage by cytotoxic antibodies. J Immunol 1970;104:1303–6.
Calich VLG, Purchio A, Paula CR. A new fluorescent viability test for fungi cells. Mycopathologia 1978;66:175–7.
Rojas-Pedral MM, Purchio A. Avaliação de eficácia do método fluorescente no estudo da viabilidade de células leveduriformes de Sporothrix schenckii. Rev Lat Am Microbiol 1988;30:197–200.
Correa B, Puchio A, Paula CR, Gambale W. Evaluation of a fluorescent method (fluorescein diacetate and ethidium bromide) in the study of the viability of Cryptococcus neoformans strains. Mycopathologia 1986;96:91–6.
Correa B, Gambale W, Paula CR, Chacon-Reche NO. Aplicação do método fluorescente (solução de diacetato de fluoresceína e brometo de etídio) no estudo da viabilidade de Metarhizium anisopliae. Rev Microbiol 1988;20:341–9.
Correa B, Purchio A, Paula CR, Gambale W. Evaluations of a fluorescent method in the study of the viability of Candida albicans strains. Rev Microbiol São Paulo 1987;18:258–63.
Correa B, Puchio A, Gambale W, Paula CR, Framil VMS. Método fluorescente para o estudo da viabilidade de células fúngicas em materiais clínicos. Rev Microbiol 1989;20:349–57.
Correa B, Gambale W, Paula CR, Palazzo S. Morphogenesis of Sporothrix schenckii “in vitro” through the method of viability by fluorescent. Appl Fluor Technol 1991;3:1–8.
Lopes MA, Fischman O, Gambale W, Correa B. Fluorescent method for studying the morphogenesis and viability of dermatophyte cells. Mycopathologia 2002;156:61–6.
Stevenson RB. Mycology guidebook Un. Seattle: Washington Press; 1974.
Jackson MA, Bennett GA. Production of fumonisin B1 by Fusarium moniliforme NRRL 13616 in submerged culture. Appl Environ Microbiol 1990;56:2296–8.
Shih C, Marth EH. Aflatoxin formation, lipid synthesis, and glucose metabolism by Aspergillus parasiticus during incubation with and without agitation. Biochem Biophys Acta 1974;338:286–96.
Lourenço A. Cinética de crescimento de cepas toxigênicas e não toxigênicas de Aspergillus flavus e produção de aflatoxina B1. São Paulo, 1996. [Dissertation]. São Paulo, São Paulo: Instituto de Ciências Biomédicas, Universidade de São Paulo.
Mahmoud ALE. Antifungical action and antiaflatoxigenic properties of some essential oil constituents. Lett Appl Microbiol 1994;19:110–3.
Volcan GS. Common cellophane wrapping as supporting substrate for the cultivations of fungi and obtention of permanent mycological preparations. Rev Inst Med Trop São Paulo 1973;15:310–5.
Carlile MJ, Watkinson SC. The fungi. London: Academic Press; 1995. 481 p.
Harris SD. Morphogenesis in germinating Fusarium graminearum macroconidia. Mycologia 2005;97(4):880–7.
Medzon EL, Brady ML. Direct measurement of acetyl esterase in living protest cells. J Bacteriol 1969;97:402–15.
Nelson PE. Taxonomy and biology of Fusarium moniliforme. Mycopathologia 1992;117:29–36.
Samson RA, Von Reenen-Hoekstra ES. Introduction to food borne fungi. 3rd ed. Baarn, Delft: Centralbureau voor Schimmelcultures; 1995. 299 p.
Leslie JF, Summerell BA. The fusarium laboratory manual. Oxford UK: Blackwell Publishing; 2006. 388 p.
Mandels GR. Kinetics of fungal growth. In: Ainswort AS, Sussman FK, editors. The fungi: an advanced treatise. New York: Academic Press; 1973.
Weinberg ED. Secondary metabolism: Raison d’être. Perspect Biol Med 1971;14:565–77.
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Granjo, C.A., dos Reis, T.A., Gambale, W. et al. Morphogenesis and growth kinetics of Fusarium verticillioides . Mycopathologia 164, 119–126 (2007). https://doi.org/10.1007/s11046-007-9035-6
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DOI: https://doi.org/10.1007/s11046-007-9035-6