Abstract
A competitive binding inhibition enzyme linked immunosorbent assay (ELISA) was used to detect Blastomyces dermatitidis antigens in urine specimens from dogs with blastomycosis. Sera from rabbits immunized with B. dermatitidis killed whole yeast cells were used as the primary antibody in the competitive ELISA. This initial study was performed to determine if B. dermatitidis antigen detection was possible and to test the efficacy of the rabbit sera as a primary antibody. An indirect ELISA was also performed to compare antigen detection in urine to antibody detection in the sera of the infected dogs. The results indicate 100% (36/36 specimens) detection of both antigen and antibody. Cross reactivity with Histoplasma capsulatum, as well as non-specific binding with the normal urine specimens, was observed with the competitive binding inhibition ELISA.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Y Al-Doory (1992) Introduction Y Al-Doory AF DiSalvo (Eds) Blastomycosis Plenum Publishing Corp New York, NY 1–8
AF DiSalvo (1992) The ecology of Blastomyces dermatitidis Y Al-Doory AF DiSalvo (Eds) Blastomycosis Plenum Publishing Corp NewYork, NY 43–69
IV JP Areno G Campbell RB George (1997) ArticleTitleDiagnosis of blastomycosis Semin Respir Infect 12 252–262 Occurrence Handle9313297
L Kaufman (1992) Immunodiagnosis of blastomycosis Y Al-Doory AF DiSalvo (Eds) Blastomycosis Plenum Publishing Corp New York, NY 123–130
SH Turner L Kaufman M Jalbert (1986) ArticleTitleDiagnostic assessment of an enzyme-linked immunosorbent assay for human and canine blastomycosis J Clin Microbiol 23 294–297 Occurrence Handle2422200
RC Axtell GM Scalarone (2002) ArticleTitleSerological differences in three Blastomyces dermatitidis strains Mycoses 45 437–442 Occurrence Handle12472717
EM Chester RC Axtell GM Scalarone (2003) ArticleTitleBlastomyces dermatitidis lysate antigens: antibody detection in serial serum specimens from dogs with blastomycosis Mycopathologia 156 289–294 Occurrence Handle10.1023/B:MYCO.0000003575.04692.2f Occurrence Handle14682453
PL Roomiany RC Axtell GM Scalarone (2002) ArticleTitleComparison of seven Blastomyces dermatitidis antigens for the detection of antibodies in humans with occupationally acquired blastomycosis Mycoses 45 282–286 Occurrence Handle10.1046/j.1439-0507.2002.00764.x Occurrence Handle12572716
PE Verweij et al. (2000) ArticleTitleClinical applications of non-culture based methods for the diagnosis and management of opportunistic and endemic mycoses Med Mycol 38 IssueID1 161–171
MM Durkin PA Connolly LJ Wheat (1997) ArticleTitleComparison of radioimmunoassay and enzyme-linked immunoassay methods for detection of Histoplasma capsulatum var. capsulatum antigen J Clin Micrbiol 35 2252–2255
TO Garringer LJ Wheat EJ Brizendine (2000) ArticleTitleComparison of an established antibody sandwich method with an inhibition method of Histoplasma capsulatum antigen detection J Clin Microbiol 38 2909–2913 Occurrence Handle10921949
BL Gomez et al. (1999) ArticleTitleDetection of the 70-Kilodalton Histoplasma capsulatum antigen in serum of histoplasmosis patients: Correlation between antigenemia and therapy during follow-up J Clin Microbiol 37 675–680 Occurrence Handle9986830
BL Gomez et al. (1997) ArticleTitleDevelopment of a novel antigen detection test for histoplasmosis J Clin Microbiol 35 2618–2622 Occurrence Handle9316918
J Wheat (1999) ArticleTitleHistoplasma capsulatum antigen detection: Comparison of the performance characteristics of a new inhibition immunoassay to those of an established antibody sandwich immunoassay J Clin Microbiol 37 2387 Occurrence Handle10408959
LJ Wheat RB Kohler RP Tewari (1986) ArticleTitleDiagnosis of disseminated histoplasmosis by detection of Histoplasma capsulatum antigen in serum and urine specimens N Engl J Med 314 83–88 Occurrence Handle3941695
J Wheat et al. (1997) ArticleTitleCross-reactivity in Histoplasma capsulatum variety capsulatum antigen assays of urine samples from patients with endemic mycoses Clin Infect Dis 24 1169–1171 Occurrence Handle9195077
HB Levine GM Scalarone SD Chaparas (1977) ArticleTitlePreparation of fungal antigens and vaccines: Studies on Coccidiodes immitis and Histoplasma capsulatum Contrib Microbiol Immun 3 106–125
HB Levine GM Scalarone GD Campbell RC Graybill SD Chaparas (1979) ArticleTitleHistoplasmin-CYL, a yeast phase reagent in skin test studies with humans Am Rev Respir Dis 119 629–636 Occurrence Handle443633
SM Johnson GM Scalarone (1989) ArticleTitlePreparation and ELISA evaluation of Blastomyces dermatitidis yeast phase lysate antigens Diagn Microbiol Infect Dis 11 81–86 Occurrence Handle10.1016/0732-8893(88)90076-4
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Shurley, J., Legendre, A. & Scalarone, G. Blastomyces Dermatitidis Antigen Detection in Urine Specimens from Dogs with Blastomycosis Using a Competitive Binding Inhibition ELISA. Mycopathologia 160, 137–142 (2005). https://doi.org/10.1007/s11046-005-3153-9
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/s11046-005-3153-9