Abstract
Apolipoprotein E (ApoE) genotypes were studied in order to determine the prevalence in the Lebanese population and compare it with other populations. DNA from 160 unrelated healthy donors from our HLA-bank was used. ApoE genotype was determined using the CardioVascular Disease (CVD) StripAssay (this assay is based on a Polymerase Chain Reaction-Reverse Hybridization technique). The prevalence of genotypes E3/3, E3/4, and E2/3 was found to be 69%, 26%, and 22%, respectively, and 0.6% for each of E2/4 and E4/4 genotypes. The Lebanese population tested showed similarities to earlier reported ApoE genotypic distributions (high E3 allele frequency) but also peculiar differences especially to some Arabic countries (total absence of E2 allele among Saudis) and other populations. This is the first report from Lebanon that will serve as a template for future investigations of the prevalence of ApoE alleles in association with various clinical entities.
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J Hardy (1995) Am. J. Med. Genet. 60 456–460 Occurrence Handle8546162 Occurrence Handle1:STN:280:BymC3MbktlA%3D Occurrence Handle10.1002/ajmg.1320600519
JC Lambert J Perez-Tur MJ Dupire D Galasko D Mann P Amouyel J Hardy A Delacourte MC Chartier-Harlin (1997) Hum. Mol. Genet. 6 2151–2154 Occurrence Handle9328480 Occurrence Handle1:CAS:528:DyaK2sXntFaqur4%3D Occurrence Handle10.1093/hmg/6.12.2151
AG Yip AC Mckee RC Green J Wells H Young LA Cupples LA Farrer (2005) Neurology 65 259–265 Occurrence Handle16043796 Occurrence Handle1:STN:280:DC%2BD2MzosFyktQ%3D%3D
G Malek LV Johnson BE Mace P Saloupis DE Schmechel DW Rickman CA Toth PM Sullivan CB Rickman (2005) Proc. Natl. Acad. Sci. USA. 102 11900–11905 Occurrence Handle16079201 Occurrence Handle1:CAS:528:DC%2BD2MXpsFGgurg%3D Occurrence Handle10.1073/pnas.0503015102
GM Teasdale GD Murray JA Nicoll (2005) Brain 128 2556–2561 Occurrence Handle16033781 Occurrence Handle1:STN:280:DC%2BD2MrnsFOrtg%3D%3D Occurrence Handle10.1093/brain/awh595
JY Um HM Kim HS Park JC Joo KY Kim SH Hong (2005) Int. J. Neurosci. 115 743–756 Occurrence Handle16019571 Occurrence Handle1:CAS:528:DC%2BD2MXlsFejsLs%3D Occurrence Handle10.1080/00207450590524421
HC Gerard KL Wildt JA Whittum-Hudson Z Lai J Ager AP Hudson (2005) Microb. Pathog. 39 19–26 Occurrence Handle15998578 Occurrence Handle1:CAS:528:DC%2BD2MXmvVGisr4%3D Occurrence Handle10.1016/j.micpath.2005.05.002
JH Zhao EJ Brunner M Kumari A Singh-Manoux E Hawe PJ Talmud MG Marmot SE Humphries (2005) Soc. Psychiatry. Psychiatr. Epidemiol. 40 557–563 Occurrence Handle16021346 Occurrence Handle1:STN:280:DC%2BD2MvitlKgsA%3D%3D Occurrence Handle10.1007/s00127-005-0925-y
MA Wozniak RF Itzhaki EB Faragher MW James SD Ryder WL Irving InstitutionalAuthorNameTrent HCV study group (2002) Hepatology 36 456–463 Occurrence Handle12143056 Occurrence Handle1:CAS:528:DC%2BD38XmsVansLc%3D Occurrence Handle10.1053/jhep.2002.34745
M Dixit S Bhattacharva B Mittal (2005) BMC. Endocr. Disord. 5 7 Occurrence Handle15992403 Occurrence Handle10.1186/1472-6823-5-7 Occurrence Handle1:CAS:528:DC%2BD2MXpsFGktr8%3D
GS Ramsaransing DJ Heersema J Kevser ParticleDe (2005) Eur. J. Neurol. 12 514–518 Occurrence Handle15958090 Occurrence Handle1:STN:280:DC%2BD2MzhtFWhuw%3D%3D Occurrence Handle10.1111/j.1468-1331.2005.01009.x
CC Hsu WH Kao J Coresh JS Pankow J Marsh-Manzi E Boerwinkle MS Bray (2005) JAMA 293 2892–2899 Occurrence Handle15956634 Occurrence Handle1:CAS:528:DC%2BD2MXltlaqsL0%3D Occurrence Handle10.1001/jama.293.23.2892
NW Chang DR Chen CT Wu BE Aouizerat FN Chen SJ Hung SH Wang MF Wei CS Chang (2005) Breast. Cancer. Res. Treat. 90 257–261 Occurrence Handle15830139 Occurrence Handle1:CAS:528:DC%2BD2MXjt1WgtLc%3D Occurrence Handle10.1007/s10549-004-4656-7
H Kohnke K Sorlin G Granath M Wadelius (2005) Eur. J. Clin. Pharmacol. 61 381–388 Occurrence Handle15952022 Occurrence Handle1:CAS:528:DC%2BD2MXmsFWlsL8%3D Occurrence Handle10.1007/s00228-005-0936-3
LE Visser PH Trienekens PA Smet ParticleDe AG Vulto A Hofman CM Duijn Particlevan BH Stricker (2005) Pharmacogenet. Genomics. 15 69–74 Occurrence Handle15861030 Occurrence Handle1:CAS:528:DC%2BD2MXivFGjt7c%3D Occurrence Handle10.1097/01213011-200502000-00002
C Lahoz EJ Schaefer LA Cupples PW Wilson D Levy D Osgood S Parpos J Pedro-Botet JA Daly JM Ordovas (2001) Atherosclerosis 154 529–537 Occurrence Handle11257253 Occurrence Handle1:CAS:528:DC%2BD3MXitVOnurk%3D Occurrence Handle10.1016/S0021-9150(00)00570-0
SL Yang BX He HL Liu ZY He H Zhang JP Luo XF Hong YC Zou (2004) Chin. Med. Sci. J. 19 150–154 Occurrence Handle15250257
ML Slattery C Sweeney M Murtaugh KN Ma JD Potter TR Levin W Samowitz R Wolff (2005) Carcinogenesis 26 1422–1429 Occurrence Handle15817610 Occurrence Handle1:CAS:528:DC%2BD2MXmsV2isbw%3D Occurrence Handle10.1093/carcin/bgi088
M Pertovaara T Lehtimaki R Rontu J Antonen A Pasternack M Hurme (2004) Rheumatology (Oxford) 43 1484–1487 Occurrence Handle1:CAS:528:DC%2BD2cXhtVanurrJ Occurrence Handle10.1093/rheumatology/keh383
P Bosco RM Gueant-Rodriguez G Anello RS Spada A Romano F Caraci R Ferri JL Gueant (2005) Neurosci. Lett. 388 IssueID3 168–172 Occurrence Handle16040191 Occurrence Handle1:CAS:528:DC%2BD2MXpslCns7g%3D
H Tang X Yan Y Hua M Wei L Zhang J Gao H Dong (2005) Zhnogua. Yi. Xue. Yi. Chuan. Xue. Za. Zhi. 22 224–226 Occurrence Handle1:CAS:528:DC%2BD2MXht1aku77O
MT Fernandez-Mestre C Yehirobi S Montagnani O Balbas Z Layrisse (2005) Dis. Markers. 21 15–19 Occurrence Handle15735320 Occurrence Handle1:CAS:528:DC%2BD2MXhsFGis7Y%3D
AA Al-Khedhairy (2004) Mol. Biol. Rep. 31 257–260 Occurrence Handle15663009 Occurrence Handle1:CAS:528:DC%2BD2MXptFWqsA%3D%3D Occurrence Handle10.1007/s11033-005-2713-x
S AL-Shammari H Fatania R Al-Radwan AO Akanji (2004) Ann. Saudi. Med. 24 361–364 Occurrence Handle15573849 Occurrence Handle1:STN:280:DC%2BD2cngtVCktQ%3D%3D
W Zhu N Feng Y Wang (2001) Zhonghua. Yu. Fang. Yi. Xue. Za. Zhi. 35 297–300 Occurrence Handle11769625 Occurrence Handle1:STN:280:DC%2BD38%2Fkt1Wiug%3D%3D
P Johannsen JK Jakobsen AL Jorgensen (1997) Ugeskr. Laeger. 159 5658–5661 Occurrence Handle9340872 Occurrence Handle1:STN:280:ByiH28zntVY%3D
T Kumar K Liestol J Maehlen A Hiorth E Jettestuen H Lind SH Brorson (2002) Hum. Biol. 74 137–142 Occurrence Handle11931575
R Benjamin A Leake FK McArthur JM Candy PG Ince JA Edwardson A Torvik CM Morris E Bjertness (1996) Neurodegeneration 5 43–47 Occurrence Handle8731381 Occurrence Handle1:STN:280:BymB1MfntVQ%3D Occurrence Handle10.1006/neur.1996.0006
M Margaglione D Seripa C Gravina E Grandone G Vecchione G Cappucci G Merla S Papa A Postiglione G Di Minno VM Fazio (1998) Stroke 29 399–403 Occurrence Handle9472880 Occurrence Handle1:STN:280:DyaK1c7jt1ahsA%3D%3D
M Muros C Rodriguez-Ferrer (1996) Atherosclerosis 121 13–21 Occurrence Handle8678918 Occurrence Handle1:CAS:528:DyaK28XhslWmurY%3D Occurrence Handle10.1016/0021-9150(95)06643-8
V Senanarong K Harnphadungkit P Lertrit C Mitrpant S Udompunthurak C Limwong N Prayoonwiwat N Poungvarin (2001) J. Med. Assoc. Thai. 84 182–187 Occurrence Handle11336076 Occurrence Handle1:STN:280:DC%2BD3M3nt1eisg%3D%3D
I Zofkova K Zajickova M Hill A Horinek (2002) Eur. J. Endocrinol. 147 503–506 Occurrence Handle12370113 Occurrence Handle1:CAS:528:DC%2BD38XotlCgtb4%3D Occurrence Handle10.1530/eje.0.1470503
R Gamboa G Hernandez-Pacheco R Hesiquio J Zuniga F Masso LF Montano M Ramos-Kuri J Estrada J Granados G Vargas-Alarcon (2000) Hum. Biol. 72 975–981 Occurrence Handle11236867 Occurrence Handle1:STN:280:DC%2BD3M7psVyjsQ%3D%3D
DP Devanand GH Pelton D Zamora X Liu MH Tabert M Goodkind N Scarmeas I Braun Y Stern R Mayeux (2005) Arch. Neurol. 62 975–980 Occurrence Handle15956169 Occurrence Handle1:STN:280:DC%2BD2MzgvFOksA%3D%3D Occurrence Handle10.1001/archneur.62.6.975
H Houlden R Crook K Duff M Hutton J Collinge P Roques M Rossor J Hardy (1995) Neurosci. Lett. 188 202–204 Occurrence Handle7609909 Occurrence Handle1:CAS:528:DyaK2MXkvVaksLw%3D Occurrence Handle10.1016/0304-3940(95)11422-S
JC Janssen JA Beck TA Campbell A Dickinson NC Fox RJ Harvey H Houlden MN Rossor J Collinge (2003) Neurology 60 235–239 Occurrence Handle12552037 Occurrence Handle1:CAS:528:DC%2BD3sXhtVGqtQ%3D%3D
EH Corder AM Saunders WJ Strittmatter DE Schmechel PC Gaskell GW Small AD Roses JL Haines MA Pericak-Vance (1993) Science 261 921–923 Occurrence Handle8346443 Occurrence Handle1:CAS:528:DyaK3sXmtVWjur8%3D
AD Roses AM Saunders EH Corder MA Pericak-Vance SH Han G Einstein C Hulette DE Schmechel M Holsti D Huang (1995) Arzneimittelforschung 45 413–417 Occurrence Handle7763336 Occurrence Handle1:CAS:528:DyaK2MXlslGksrg%3D
MC Chartier-Harlin M Parfitt S Legrain J Perez-Tur T Brousseau A Evans C Berr O Vidal P Roques V Gourlet (1994) Hum. Mol. Genet. 3 569–574 Occurrence Handle8069300 Occurrence Handle1:STN:280:ByuA2cfltVY%3D
Y Lee M Aono D Laskowitz DS Warner RD Pearlstein (2004) Neurochem. Int. 44 107–118 Occurrence Handle12971913 Occurrence Handle1:CAS:528:DC%2BD3sXntVGmsr8%3D Occurrence Handle10.1016/S0197-0186(03)00112-8
EH Corder JF Ervin E Lockhart MH Szymanski DE Schmechel CM Hulette (2005) J. Biomed. Biotechnol. 2005 189–197 Occurrence Handle16046825 Occurrence Handle10.1155/JBB.2005.189 Occurrence Handle1:CAS:528:DC%2BD2MXht1yltLzM
M Bednarska-Makaruk M Rodo C Markuszewski A Rozenfeld M Swiderska B Habrat H Wehr (2005) Alcohol. Alcohol. 40 274–282 Occurrence Handle15809232 Occurrence Handle1:CAS:528:DC%2BD2MXltVKmt7Y%3D
D Corella K Tucker C Lahoz O Coltell LA Cupples PW Wilson EJ Schaefer JM Ordovas (2001) Am. J. Clin. Nutr. 73 736–745 Occurrence Handle11273848 Occurrence Handle1:CAS:528:DC%2BD3MXisFWhsrk%3D
S Al-Shammri H Fatania R Al-Radwan AO Akanji (2005) Clin. Chim. Acta. 351 203–207 Occurrence Handle15563891 Occurrence Handle1:CAS:528:DC%2BD2cXhtVant7rJ Occurrence Handle10.1016/j.cccn.2004.09.015
E Cocco A Sotgiu G Costa MR Murru C Mancosu R Murru M Lai P Contu MG Marrosu (2005) Neurology 64 564–566 Occurrence Handle15699400 Occurrence Handle1:STN:280:DC%2BD2M%2FmvF2rsA%3D%3D
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Mahfouz, R.A.R., Sabbagh, A.S., Zahed, L.F. et al. Apolipoprotein E Gene Polymorphism and Allele Frequencies in the Lebanese Population. Mol Biol Rep 33, 145–149 (2006). https://doi.org/10.1007/s11033-006-6260-x
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DOI: https://doi.org/10.1007/s11033-006-6260-x