Abstract
Lysosomal cysteine protease cathepsin B has been reported to play an important role in apoptosis of many different cancer cells, but the regulation of cathepsin B in apoptosis is poorly understood. Human homologue of SETA binding protein 1 (hSB1) was identified to interact with cathepsin B by yeast-two hybrid method, and the interaction was confirmed in vitro GST pull-down assay and in vivo coimmunoprecipitation experiment. hSB1 was co-localized with cathepsin B in cellular lysosomes. Our previous study has shown that TNF can induce ovarian cancer cells OV-90 apoptosis and the apoptosis process is cathepsin B-depended. Here we provide evidence that overexpression of cathepsin B-interacting protein hSB1 could suppress TNF-triggered apoptosis in OV-90 cells, but has no effect on cellular cathepsin B activity. hSB1 may function as a regulator of cathepsin B-mediated apoptosis.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Thompson CB: Apoptosis in the pathogenesis and treatment of disease. Science 267: 1456–1462, 1995
Leist M, Jaattela M: Four deaths and a funeral: from caspases to alternative mechanisms. Nat Rev Mol Cell Biol 2: 589–598, 2001
Guicciardi ME, Leist M, Gores GJ: Lysosomes in cell death. Oncogene 23: 2881–2890, 2004
Liu ZG: Molecular mechanism of TNF signaling and beyond. Cell Res 15: 24–27, 2005
Cuicciardi ME, Deussing J, Miyoshi H, Bronk SF, Svingen PA, Peters C, Kaufmann SH, Gores GJ: Cathepsin B contributes to TNF-α-mediated hepatocyte apoptosis by promoting mitochondrial release of cytochrome c. J Clin Invest 106: 1127–1137, 2000
Werneburg N, Guicciardi ME, Yin XM, Gores GJ: TNF-α-mediated lysosomal permeabilization is FAN and caspase 8/Bid dependent. Am J Physiol Gastrointest Liver Physiol 287: G436–G443, 2004
Foghsgaard L, Wissing D, Mauch D, Lademann U, Bastholm L, Boes M, Elling F, Leist M, Jaattela M: Cathepsin B acts as a dominant execution protease in tumor cell apoptosis induced by tumor necrosis factor. J Cell Biol 153: 999–1009, 2001
Liu JP, Guo Q, Chen BX, Yu Y, Lu H, Li YY: Cathepsin B and its interacting proteins, bikunin and TSRC1, correlate with TNF-induced apoptosis of ovarian cancer cells OV-90. FEBS Lett 580: 245–250, 2006
Bardwell VJ, Treisman R: The POZ domain: a conserved protein-protein interaction motif. Genes Dev 8: 1664–1677, 1994
Smith TF, Gaiatzes CG, Saxena K, Neer EJ: The WD repeat: a common architecture for diverse functions. Trends Biochem Sci 24: 181–185, 1999
Ye XX, Lu H, Yu Y, Ding N, Zhang NL, Huo KK, Wan DF, Li YY, Gu JR: PP5644 interacts with phosphatidylinositol-4-phosphate adaptor protein-1 associated protein-1. Mol Cell Biochem 271: 151–158, 2005
Mund T, Gewies A, Schoenfeld N, Bauer MK, Grimm S: Spike, a novel BH3-only protein, regulates apoptosis at the endoplasmic reticulum. FASEB J 17: 696–698, 2003
Bogler O, Furnari FB, Kindler-Roehrborn A, Sykes VW, Yung R, Huang HJS, Cavenee WK: SETA: a novel SH3 domain containing adapter molecule associated with malignancy in astrocytes. Neuro-Oncology 2: 6–15, 2000
Borinstein SC, Hyatt MA, Sykes VW, Straub RE, Lipkowitz S, Boulter J, Bogler O: SETA is a multifunctional adapter protein with three SH3 domains that binds Grb2, Cbl and the novel SB1 proteins. Cell Signal 12: 769–779, 2000
Gout I, Middleton G, Adu J, Ninkina NN, Drobot LB, Filonenko V, Matsuka G, Davies AM, Waterfield M, Buchman VL: Negative regulation of PI 3-kinase by Ruk, a novel adaptor protein. EMBO J 19: 4015–4025, 2000
Chen B, Borinstein SC, Gillis J, Sykes VW, Bogler O: The glioma-associated protein SETA interacts with AIP1/Alix and ALG-2 and modulates apoptosis in astrocytes. J Biol Chem 275: 19275–19281, 2000
Haglund K, Shimokawa N, Szymkiewicz I, Dikic I: Cbl-directed monoubiquitination of CIN85 is involved in regulation of ligand induced degradation of EGF receptors. Proc Natl Acad Sci USA 99: 12191–12196, 2002
Schmidt MH, Chen B, Randazzo LM, Bogler O: SETA/CIN85/Ruk and its binding partner AIP1 associate with diverse cytoskeletal elements, including FAKs, and modulate cell adhesion. J Cell Sci 116: 2845–2855, 2003
Narita T, Nishimura T, Yoshizaki K, Taniyama T: CIN85 associates with TNF receptor 1 via Src and modulates TNF-a-induced apoptosis. Experimental Cell Research 304: 256–264, 2005
Podgorski I, Sloane BF: Cathepsin B and its role(s) in cancer progression. Biochem Soc Symp 70: 263–276, 2003
Berdowska I: Cysteine proteases as disease markers. Clinica Chimica Acta 342: 41–69, 2004
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Liu, JP., Liu, NS., Yuan, HY. et al. Human Homologue of Seta Binding Protein 1 Interacts with Cathepsin B and Participates in TNF-Induced Apoptosis in Ovarian Cancer Cells. Mol Cell Biochem 292, 189–195 (2006). https://doi.org/10.1007/s11010-006-9214-7
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11010-006-9214-7