Abstract
This study investigated the expression of CD40, CD40 ligand (CD40L) and matrix metalloproteinases (MMPs) in dietary-induced atherosclerosis in rats. Wister rats were fed with high cholesterol diet (As group, n = 6) or with normal diet (N group, n = 6). Blood cells that express CD40 and CD40L were sorted by flow cytometry, the MMP-2 and MMP-9 were measured by zymography method. The morphological locations of MMP-2 and MMP-9 in the aorta were studied with immunohistochemistry and by microscopy. The results showed that the expression of CD40, CD40L and matrix metalloproteinase were higher in As group than those in control group. The MMP-2 and MMP-9 were positive in As group but negative in control group by immunohistochemistry study. Our results suggest that the expression of CD40 and CD40L in the blood cells and the activities of MMP-2 and MMP-9 in plasma were higher in As group than those in Normal group, indicating that they may contribute to the formation of atherosclerosis.
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Abbreviations
- CD40L:
-
CD40 ligand
- DAB:
-
diaminobenzidine
- FACS:
-
fluorescence-activated cell sorter
- FITC:
-
fluorescence isothiocyanate
- IL:
-
interleukin
- MCP-1:
-
monocyte chemoattractant protein-1
- MMP:
-
matrix metalloproteinase
- PBS:
-
phosphate-buffered saline
- TF:
-
tissue factor
- TNF:
-
tumor necrosis factor
References
Spronk HM, van der Voort D, Ten Cate H: Blood coagulation and the risk of atherothrombosis: A complex relationship. Thromb J 2: 12–12, 2004
Nijm J, Wikby A, Tompa A, Olsson AG, Jonasson L: Circulating levels of proinflammatory cytokines and neutrophil–platelet aggregates in patients with coronary artery disease. Am J Cardiol 95: 452–456, 2005
Dardik A, Yamashita A, Aziz F, Asada H, Sumpio BE: Shear stress-stimulated endothelial cells induce smooth muscle cell chemotaxis via platelet-derived growth factor-BB and interleukin-1alpha. J Vasc Surg 41: 321–331, 2005
Luedemann J, Schminke U, Berger K, Piek M, Willich SN, Doring A, John U, Kessler C: Association between behavior-dependent cardiovascular risk factors and asymptomatic carotid atherosclerosis in a general population. Stroke 33: 2929–2935, 2002
Mullenix PS, Andersen CA, Starnes BW: Atherosclerosis as inflammation. Ann Vasc Surg 19: 130–138, 2005
Jiang X, Zeng HS, Guo Y, Zhou ZB, Tang BS, Li FK: The expression of matrix metalloproteinases-9, transforming growth factor-beta1 and transforming growth factor-beta receptor I in human atherosclerotic plaque and their relationship with plaque stability. Chin Med J (Engl) 117: 1825–1829, 2004
Grewal IS, Foellmer HG, Grewal KD, Xu J, Hardardottir F, Baron JL, Janeway CA Jr, Flavell RA: Requirement for CD40 ligand in costimulation induction, T cell activation, and experimental allergic encephalomyelitis. Science 273: 1864–1867, 1996
Mott JD, Werb Z: Regulation of matrix biology by matrix metalloproteinases. Curr Opin Cell Biol 16: 558–564, 2004
Randeva HS, Lewandowski, KC, Komorowski J, Murray RD, O'Callaghan CJ, Hillhouse EW, Stepien H, Shalet SM: Growth hormone replacement decreases plasma levels of matrix metalloproteinases (2 and 9) and vascular endothelial growth factor in growth hormone-deficient individuals. Circulation 109: 2405–2410, 2004
Mach F, Schonbeck U, Sukhova GK, Bourcier T, Bonnefoy JY, Pober JS, Libby P: Functional CD40 ligand is expressed on human vascular endothelial cells, smooth muscle cells, and macrophages: Implications for CD40-CD40 ligand signaling in atherosclerosis. Proc Natl Acad Sci USA 94: 1931–1936, 1997
Mazzone A, De Servi S, Ricevuti G, Mazzucchelli I, Fossati G, Pasotti D, Bramucci E, Angoli L, Marsico F, Specchia G: Increased expression of neutrophil and monocyte adhesion molecules in unstable coronary artery disease. Circulation 88: 358–363, 1993
Forsyth PA, Wong H, Laing TD, Rewcastle NB, Morris DG, Muzik H, Leco KJ, Johnston RN, Brasher PM, Sutherland G, Edwards DR: Gelatinase-A (MMP-2), gelatinase-B (MMP-9) and membrane type matrix metalloproteinase-1 (MT1-MMP) are involved in different aspects of the pathophysiology of malignant gliomas. Br J Cancer 79: 1828–1835, 1999
French LE, Huard B, Wysocka M, Shane R, Contassot E, Arrighi JF, Piguet V, Calderara S, Rook AH: Impaired CD40L signaling is a cause of defective IL-12 and TNF-alpha production in Sezary syndrome: Circumvention by hexameric soluble CD40L. Blood 105: 219–225, 2005
Davies MJ: Reactive oxygen species, metalloproteinases, and plaque stability. Circulation 97: 2382–2383, 1998
Schonbeck U, Mach F, Sukhova GK, Murphy C, Bonnefoy JY, Fabunmi RP, Libby P: Regulation of matrix metalloproteinase expression in human vascular smooth muscle cells by T lymphocytes: A role for CD40 signaling in plaque rupture? Circ Res 81: 448–454, 1997
Eckart RE, Uyehara CF, Shry EA, Furgerson JL, Krasuski RA: Matrix metalloproteinases in patients with myocardial infarction and percutaneous revascularization. J Interv Cardiol 17: 27–31, 2004
Wang Y, Blessing F, Walli AK, Uberfuhr P, Fraunberger P, Seidel D: Effects of heparin-mediated extracorporeal low-density lipoprotein precipitation beyond lowering proatherogenic lipoproteins – reduction of circulating proinflammatory and procoagulatory markers. Atherosclerosis 175: 145–150, 2004
Libby P, Mach F, Schonbeck U, Bourcier T, Aikawa M: Regulation of the thrombotic potential of atheroma. Thromb Haemost 82: 736–741, 1999
Schonbeck U, Sukhova GK, Shimizu K, Mach F, Libby P: Inhibition of CD40 signaling limits evolution of established atherosclerosis in mice. Proc Natl Acad Sci USA 97: 7458–7463, 2000
Galang N, Sasaki H, Maulik N: Apoptotic cell death during ischemia/reperfusion and its attenuation by antioxidant therapy. Toxicology 148: 111–118, 2000
Ferroni P, Basili S, Martini F, Cardarello CM, Ceci F, Di Franco M, Bertazzoni G, Gazzaniga PP, Alessandri C: Serum metalloproteinase 9 levels in patients with coronary artery disease: A novel marker of inflammation. J Investig Med 51: 295–300, 2003
Schonbeck U, Mach F, Sukhova GK, Atkinson E, Levesque E, Herman M, Graber P, Basset P, Libby P: Expression of stromelysin-3 in atherosclerotic lesions: Regulation via CD40-CD40 ligand signaling in vitro and in vivo. J Exp Med 189: 843–853, 1999
Li D, Liu L, Chen H, Sawamura T, Mehta JL: LOX-1, an oxidized LDL endothelial receptor, induces CD40/CD40L signaling in human coronary artery endothelial cells. Arterioscler Thromb Vasc Biol 23: 816–821, 2003
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Wu, M., Li, YG. The expression of CD40-CD40L and activities of matrix metalloproteinases in atherosclerotic rats. Mol Cell Biochem 282, 141–146 (2006). https://doi.org/10.1007/s11010-006-1741-8
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DOI: https://doi.org/10.1007/s11010-006-1741-8