Abstract
We report the first findings of Metal-Enhanced Fluorescence (MEF) from modified plastic substrates. In the past several years our laboratories have reported the favorable effects of fluorophores in close proximity to silver nanoparticles. These effects include, enhanced fluorescence intensities, (increased detectability), and reduced lifetimes, (enhanced fluorophore photostability). All of these reports have featured silver nanostructures and fluorophores which have been immobilized onto clean glass or quartz surfaces. In this report we show how plastic surfaces can be modified to obtain surface functionality, which in turn allows for silver deposition and therefore metal-enhanced fluorescence of fluorophores positioned above the silver using a protein spacer. Our findings show that plastic substrates are ideal surfaces for metal-enhanced phenomena, producing similar enhancements as compared to clean glass surfaces. Subsequently, we speculate that plastic substrates for MEF will find common place, as compared to the more expensive and less versatile traditional silica based supports.
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References
C. D. Geddes and J. R. Lakowicz (2002). Metal-enhanced fluorescence. J. Fluoresc. 12(2), 121–129.
J. R. Lakowicz (2001). Radiative decay engineering: Biophysical and biomedical applications. Appl. Biochem. 298, 1–24.
J. R. Lakowicz, Y. Shen, S. D’Auria, J. Malicka, J. Fang, Z. Grcyzynski, and I. Gryczynski (2002). Radiative decay engineering 2. Effects of silver island films on fluorescence intensity, lifetimes, and resonance energy transfer, Anal. Biochem. 301, 261–277.
C. D. Geddes, H. Cao, I. Gryczynski, Z. Gryczynski, J. Fang, and J. R. Lakowicz (2003). Metal-enhanced fluorescence due to silver colloids on a planar surface: Potential applications of Indocyanine green to in vivo imaging. J. Phys. Chem. A 107, 3443–3449.
C. D. Geddes, K. Aslan, I. Gryczynski, J. Malicka, and J. R. Lakowicz (2004) in C. D. Geddes (Ed.) Noble Metal Nanostructure for Metal-Enhanced Fluorescence, Reviews in Fluorescence 2004, Springer, New York, pp. 365–401.
Y. Liu, D. Ganser, A. Schneider, P. Grodzinski, and N. Kroutchinina (2001). Microfabricated polycarbonate CE devices for DNA analysis. Anal. Chem. 73, 4196–4201.
M. Boerner, M. Kohl, F. Pantenburg, W. Bacher, H. Hein, and W. Schomburg (1996). Microsyst. Technol. 2, 149–152.
M. A. Roberts, J. S. Rossier, P. Bercier, and H. Girault (1997). UV laser machined polymer substrates for the development of microdiagnostic systems. Anal. Chem. 69, 2035–2042.
L. Martynova, L. E. Locascio, M. Gaitan, G. W. Kramer, R. G. Christensen, and W. MacCrehan (1997). Fabrication of plastic microfluid channels by imprinting methods. Anal. Chem. 69, 4783–4789.
J. Xu, L. Locascio, M. Gaitan, and C. S. Lee (2000). Room-temperature imprinting method for plastic microchannel fabrication. Anal. Chem. 72, 1930–1933.
R. M. McCormick, R. J. Nelson, M. G. Alonso-Amigo, D. J. Benvegnu, and H. H. Hooper (1997). Microchannel electrophoretic separations of DNA in injection-molded plastic substrates. Anal. Chem. 69, 2626–2630.
L. Dauginet, A.-S. Duwez, R. Legras, and S. Demoustier-Champagne. Surface modification of polycarbonate and poly(ethyleneterephthalate) films and membranes by polyelectrolyte deposition. Langmuir 17, 3952–3957.
Y. Xu, B. Vaidya, A. B. Patel, S. M. Ford, R. L. McCarley, and S. A. Soper (2003). Solid-phase reversible immobilization in microfluidic chips for the purification of dye-labeled DNA sequencing fragments. Anal. Chem. 75, 2975–2984.
J. R. Lakowicz (1999). Principles of Fluorescence Spectroscopy, Kluwer, New York.
W. Ward and T. J. McCarthy (1989) in H. F Mark, N. M. Bikales, C. G. Overberger, G. Menges, and J. I. Kroschwitz (Eds.), Encyclopedia of Polymer Science and Engineering, 2nd ed. John Wiley and Sons, New York, 1989, suppl. vol. pp. 674–689.
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Aslan, K., Badugu, R., Lakowicz, J.R. et al. Metal-Enhanced Fluorescence from Plastic Substrates. J Fluoresc 15, 99–104 (2005). https://doi.org/10.1007/s10895-005-2515-5
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DOI: https://doi.org/10.1007/s10895-005-2515-5