Abstract
To evaluate the potential of β-Ti20Mo alloy as a dental material, we tested its corrosion behaviour in artificial saliva in comparison to that of cp-Ti. Open-circuit potential (EOC), potentiodynamic polarization and electrochemical impedance spectroscopy (EIS) were used as electrochemical methods to characterize the corrosion behaviour of Ti20Mo alloy and cp-Ti, respectively. Corrosion current and passive current densities obtained from the polarization curves showed low values indicating a typical passive behaviour for Ti20Mo alloy. The EIS technique enabled us to study the nature of the passive film formed on the binary Ti20Mo alloy at various imposed potentials. The Bode phase spectra obtained for Ti20Mo alloy in artificial saliva exhibited two-time constants at higher potential (0.5 V, 1.0 V), indicating a two-layer structure. According to our experimental measurements, Ti20Mo alloy appears to possess superior corrosion resistance to that of cp-Ti in artificial saliva.
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1 Introduction
Titanium and titanium alloys are widely used for structural biomedical and dental applications due to their low density, excellent biocompatibility, corrosion resistance and suitable mechanical properties [1–10].
A stable, adherent, compact and passive layer, consisting mainly of TiO2 oxide, forms naturally on the surface of titanium and its alloys. This titanium oxide, an n-type semiconductor, has only ionic conductivity, and it is responsible for the high corrosion resistance of titanium and its alloys in various electrochemical media [11–13]. Thus, this oxide layer has an important role in terms of the biocompatibility of titanium-based biomaterials [14].
Due to its poor mechanical properties, cp-Ti is inadequate for most biomedical and dental applications so that it became necessary to use its alloys for such applications. Titanium alloys containing aluminium and vanadium elements exhibit higher strength than cp-Ti [15]. For this reason, Ti6Al4V alloy composition was the first titanium alloy used as implant material [16]. Further studies have indicated that vanadium, used to stabilize the β-phase, produces harmful oxides for the human body [17, 18]. The toxicity of vanadium pushed forward the development of new Ti-based alloys designed to replace Ti6Al4V. Khan et al. suggested [19] that Ti6Al7Nb could be a better alternative to Ti6Al4V due to its better corrosion resistance and stability in simulated body fluid environment with different pH values. Moreover, there have been concerns, not yet confirmed, about the connection between Al and Alzheimer disease [19, 20].
Nb, Ta, Zr, Mo and Sn have been selected as safe alloying elements to titanium, since they are judged as non-toxic and non-allergic [21]. In addition, alloying titanium with isomorphous β-stabilizers such as Mo, Nb and Ta is known to improve the mechanical properties [22, 23]. Therefore, new V and Al free binary alloys, having non-toxic elements, such as Ti45Nb [24], Ti35Nb [25], TiHf [26], TiTa [27], TiMo [28–32] have been developed in the last decades. Due to its high β-stabilizer power, the Mo element was recently used as allowing element and different compositions, such as Ti15Mo (%wt.) [28], Ti10Mo (%wt.) [29], Ti(4÷20)Mo (%wt.) [30, 31], Ti(5÷35)Mo (%wt.) [33], were investigated in view of their biomedical and dental applications.
In the pre-clinical testing of the metallic biomaterials used in dental applications, the in vitro corrosion behaviour tests in different artificial saliva media have a well-defined importance. Human saliva can vary to a considerable degree and is dependent on the age and sex of the patient, the time of day, eating habits, medication and oral hygiene [34]. In this study, we selected Fusayama’s artificial saliva because the results obtained with this medium are consistent with the clinical experience of dental alloys [35].
The corrosion behaviour of β-Ti20Mo (%wt.) alloy in Fusayama’s artificial saliva has been investigated in comparison to commercial pure titanium (cp-Ti) using potential-time measurements, potentiodynamic polarization and EIS measurements.
2 Materials and methods
2.1 Materials
Cold crucible levitation melting (CCLM) (Metallurgical Chemistry Laboratory, INSA Rennes, France) in a high frequency induction furnace under a pure argon atmosphere stabilized after several cycles of argon charging-high vacuum pumping, was the technique used to synthesize small ingots of Ti20Mo alloy. In order to eliminate segregation, the Ti20Mo alloy ingots were homogenized by heat treatment performed in a tubular furnace under high-vacuum (10−7 ÷ 10−6 mbar) using the following conditions: (1) heating from room temperature at 5°C/min, (2) homogenization at a β-transus temperature + 100°C for 24 h and (3) natural cooling. The synthesis and structural characterization of the β-type Ti alloys containing Mo as β-stabilizer element were detailed elsewhere [36]. The cp-Ti samples were made from a bar stock in annealed state (delivered by R&D Consultanta si Servicii SRL, Bucharest, Romania).
2.2 Corrosion tests
2.2.1 Corrosion medium
Fresh Fusayama’s artificial saliva [37] used as corrosion medium, was prepared before use. It is composed of 0.400 g NaCl; 0.400 g KCl; 0.795 g CaCl2·2H2O; 0.780 g NaH2PO4·2H2O; 0.005 g Na2S·9H2O; 1.000 g NH2CONH2 and distilled water up to 1000 mL. The solution has a pH value of about 5.6. Because the short-term pH variations of human saliva include the intake of acidic beverages (pH 2–3) and secretion of gastric acid (pH 1), we added lactic acid to Fusayama’s artificial saliva to decrease the pH value to 2.5 in order to accentuate the corrosion.
2.2.2 Corrosion setup
Electrochemical measurements were carried out in aerated artificial saliva at 25°C using a Princeton Applied Research potentiostat (Model 263 A) connected to a Princeton Applied Research 5210 lock-in amplifier. The electrochemical system was controlled by a personal computer with a specific software package called Electrochemistry Power Suite (Princeton Applied Research).
All the electrochemical measurements were performed using a conventional three-electrode system. Working electrodes made from both the Ti20Mo alloy and cp-Ti samples and a platinum sheet as counter electrode and a saturated calomel electrode (SCE) as reference electrode were used. All potentials are referred to SCE in this article. The working electrode was prepared by attaching a brass nut to one side of the specimen with conductive resin. Moreover, the preparation of the samples made it possible to leave a surface of 0.2 cm2 free. Each surface was polished with emery sheets (from 600 to 2000 grit) and was then mirror-polished by means of 1 μm alumina aqueous solution. Afterwards, each surface was cleaned using methanol and distilled water.
2.2.3 Open-circuit potential
The open-circuit potential (EOC) measurement of each specimen was first recorded for 24 h after immersion in each electrolyte. This period seemed sufficient to attain steady-state conditions.
2.2.4 Potentiodynamic polarization
The potentiodynamic polarization curve was recorded by means of a potential scanning rate of 0.5 × 10−3 V/s from − 0.8 V to +1.5 V, and reversed in the negative direction to +1.0 V. We used the automatic data acquisition system to plot the potentiodynamic polarization curves. Both the anodic and cathodic branches of the Tafel plots were useful to estimate the corrosion rate and potential.
2.2.5 Electrochemical impedance spectroscopy
Aerated artificial saliva (25°C) subjected to the following different potentials: -0.5 V (approaching the ZCP value for both titanium-based materials), 0.0 V, 0.5 V and 1.0 V, was used to perform EIS measurements. The EIS results were recorded in the 10−2–105 Hz frequency range. The over potential was applied after 30 min. The amplitude of the alternative potential applied was 10−2 V.
In order to supply quantitative support for discussions on these experimental EIS results, an appropriate model (ZSimpWin-PAR, USA) for equivalent circuit (EC) quantification was used.
3 Results and discussion
3.1 Open-circuit potential
Figure 1 shows the open-circuit potential (EOC) versus time behaviour of Ti20Mo alloy and cp-Ti in artificial saliva.
After fresh surface polishing of Ti20Mo alloy and cp-Ti exposed to humid air, a thin oxide layer is spontaneously formed on their surfaces. After the samples’ immersion into the electrolyte, the open circuit potential increases rapidly with time. As concerns Ti20Mo alloy, the initial open circuit potential is around −0.600 V and after approximately 1 h of immersion, this value rises to about −0.400 V. This initial EOC increase seems to be related to the thickening of the oxide film, which improves its corrosion protection ability [7]. Afterwards, the EOC value increases slowly suggesting the growth of the film onto the metallic surface [10]. The potential was stable from 10 h onwards. The samples from cp-Ti have a similar behaviour. However, cp–Ti shows nobler potential than Ti20Mo alloy probably due to the formation of a pure TiO2 oxide film, as compared to the mixed oxide formation on Ti20Mo alloy possibly consisting of TiO2 and Mo2O5 [31]. The samples of both titanium-based materials did not exhibit potential drops associated with surface activation during the 24 h exposure in artificial saliva. This kind of behaviour suggests that the air-formed native oxide is kinetically resistant to chemical dissolution in artificial saliva.
3.2 Potentiodynamic polarization
Figure 2 shows the polarization curves of Ti20Mo alloy and cp-Ti in artificial saliva. Prior to polarization measurement, the electrode was cathodically polarized at −1.5 V in the working solution for 60 s in order to remove any spontaneously formed surface film [11].
PowerCorr software (PAR, USA) was used to extract the zero current potential (ZCP) and corrosion current density (Jcorr) values from the potentiodynamic polarization plot. Both samples reach their respective stable passive current densities as the potential increases. Passive current density (Jpass) was also determined from the potentiodynamic anodic diagram at different potentials (0.0, 0.5 and 1.0 V). The ZCP, Jcorr and Jpass values from two different polarization curves are shown in Table 1. Figure 2 clearly proves that Ti20Mo alloy possesses a corrosion resistance superior to that of cp-Ti in artificial saliva. As for Ti20Mo alloy, the passive current density is nearly stable when the potential increases from 0.0 V to 1.0 V.
The current exhibited a negative hysteresis when the scan was reversed and no pits were found on the samples after the polarization test.
Passive current densities (Jpass) are higher than corrosion current densities (Jcorr) suggesting that the protective Ti oxide film can be more defective [7, 15]. The lower corrosion current densities and the lower and more stable passive current densities of Ti20Mo alloy indicate that Ti20Mo alloy possesses a better corrosion resistance than the cp-Ti in artificial saliva.
3.3 Electrochemical impedance spectroscopy
EIS data contain information on the passive film characteristics. The fitting quality of EIS data was estimated by both the chi-square (χ2) test (between 10−5 and 10−4) values and the comparison between error distribution versus frequency values (± 2% for the whole frequency range) corresponding to experimental and simulated data. The chi-square (χ2) and error distribution versus frequency values point to the excellent agreement between the experimental and simulated values.
The impedance spectroscopy results for Ti20Mo alloy and cp-Ti in artificial saliva at different selected potentials are shown in the Bode diagrams in Figs. 3, 4, 5, and 6. The phase angle maxima noticed for Ti20Mo alloy and cp-Ti lie in the range of approximately −70° to −80°. The phase angle values, between −70° and −80° in a large frequency range (0.1÷100 Hz) both for cp-Ti (−0.5 V) and Ti20Mo alloy (−0.5 V and 0.0 V), are characteristic values of a compact passive film [4]. The high impedance values (105 Ω cm2 order) obtained from medium to low frequencies for these samples suggest a high corrosion resistance in artificial saliva.
The phase angle plots (Figs. 3 and 5) reveal only one peak both for cp-Ti at −0.5 V and Ti20Mo alloy at −0.5 V and 0.0 V, indicating the involvement of a single time constant. This behaviour usually indicated that a thin passive oxide film was formed on the surface [38]. The results of the analysis are shown in Table 2. The simple equivalent circuit (EC) consisting of only one time constant (Fig. 7a) was used to model the experimental impedance spectra. A good agreement between the experimental data and fitted data was obtained. The RbL and QbL parameters describe the processes occurring at the electrolyte/dense passive film interface. Rsol is the ohmic resistance of the electrolyte. The same Rsol value, that is 53 ± 3 Ω, was noticed in all the cases. Studies performed on various Ti-based alloys under physiological conditions showed that this simple EC could be used successfully to describe the corrosion behaviour of titanium alloys [4, 8, 31, 39, 40].
The Q symbol reveals the possibility of a non-ideal capacitance (CPE, constant phase element). The CPE relies on the fact that, experimentally, the barrier film never exhibits the theoretically expected phase shift of −90° and a slope of −1 for an ideal dielectric. The following equation [41] calculates the CPE impedance:
where, when n = 1, the Q element is reduced to a capacitor with a capacitance C, and when n = 0, it is reduced to a simple resistor. This n is related to a slope of the Log (Z mod) versus Log (Frequency) Bode–plots, ω is the angular frequency and j is an imaginary number (j 2 = −1).
The barrier layer resistance of Ti20Mo alloy at −0.5 V is about two times higher than that of cp-Ti. The 0.8 nbL value indicates the deviation from a near capacitance behaviour of both titanium-based materials at −0.5 V and of Ti20Mo alloy at 0.0 V.
The impedance spectra fitting for Ti20Mo alloy at 0.5 V and 1.0 V and for cp-Ti at 0.0 V, 0.5 V and 1.0 V was carried out with an EC (Fig. 7b) using a series combination of the Rsol solution resistance, with two RQ elements in parallel: Rsol(RoL(QoL(RbLQbL))). Again, a very good agreement between the simulated and experimental data was obtained. The Rsol (RbLQbL) model failed to provide a satisfactory fit (the values of the chi-square (χ2) test were about 10−2). Table 2 shows the results of the fittings.
This EC is based on a model used by Pan et al. [3] to simulate data in saline solution. These authors noticed that the surface layer formed on titanium is in fact a two-layer oxide: an inner barrier layer and an outer porous layer. Studies performed on Ti-based alloys under physiological conditions showed that the EC proposed by Pan et al. [3] successfully describes the behaviour of these materials as well [4, 7, 10, 13, 42]. The high-frequency RoL and QoL parameters are the properties of the reactions at the outer porous passive film/solution interface. The RbL and QbL parameters describe the processes occurring to the electrolyte/dense passive film interface.
High resistance values of the outer layer (105 Ω cm2 order) were found for both samples. These values indicate the formation of a porous layer with a good corrosion resistance [7].
As for Ti20Mo alloy, barrier layer resistance increases as the potential increase from −0.5 V to 0.5 V, and then decreases slowly as the potential increases to 1.0 V. The resistance decrease indicates that the oxide layer may become more defective at high over potential [7, 15]. However, the RbL of Ti20Mo alloy in artificial saliva was still high at 1.0 V as seen in Table 2. This indicates that the alloy is highly resistant to corrosion even at very high over potentials. As concerns cp-Ti, as potential increases from 0.0 V to 1.0 V, barrier layer resistance remains approximately constant (around 5 × 105 Ω cm2), probably due to the fact that the titanium oxide is essentially TiO2 [3]. The higher RbL of Ti20Mo alloy for all the potentials indicates that this alloy possesses better corrosion resistance than cp-Ti in artificial saliva, supporting the results obtained from the polarization tests. For both samples, the RbL (inner barrier layer) is about 2 ÷ 5 times higher than the RoL (porous layer). This reveals that the inner barrier layer provides most of the protection.
However, the RoL value is high (105 Ω cm2 order) for both samples. It may be possible that the lactic acid molecules were adsorbed on the sample surface during the polarization, which would explain the increase in the RoL resistance.
Also, it is worthy to note that the nbL values, corresponding to 0.5 V and at 1.0 V for both samples, were found to be around 0.9. This indicates that the film exhibits a near capacitive behaviour.
4 Conclusions
We showed that electrochemical techniques play an overwhelming role in evaluating corrosion resistance. Considering the electrochemical measurements, the following conclusions could be drawn:
-
1.
A passive film was formed on Ti20Mo alloy during the corrosion tests carried out in Fusayama’s artificial saliva. Its formation was accompanied by very low experimental current densities.
-
2.
The Bode phase spectra recorded for Ti20Mo alloy in Fusayama’s artificial saliva exhibited single-time constant at lower potential (−0.5 V, 0.0 V), indicating a single layer structure. Bode phase spectra for both samples showed two-time constant at higher potential (0.5 V, 1.0 V) and were fitted with an equivalent circuit (EC) characterized by two RQ elements in parallel. This EC is consistent with the model of a two-layer structure for passive films: an inner barrier layer with high impedance, responsible for corrosion protection, and an outer porous layer. Ti20Mo alloy appears to possess superior corrosion resistance to that of cp-Ti in artificial saliva.
-
3.
The corrosion parameters experimentally obtained for the Ti20Mo alloy in Fusayama’s artificial saliva suggest that this material has dental use potential.
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Mareci, D., Chelariu, R., Dan, I. et al. Corrosion behaviour of β-Ti20Mo alloy in artificial saliva. J Mater Sci: Mater Med 21, 2907–2913 (2010). https://doi.org/10.1007/s10856-010-4147-9
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DOI: https://doi.org/10.1007/s10856-010-4147-9