Abstract
This paper examines the effects of nano-scale titanium coatings, and micro-groove/micro-grid patterns on cell/surface interactions on silicon surfaces. The nature of the cellular attachment and adhesion to the coated/uncoated micro-textured surfaces was elucidated by the visualization of the cells and relevant cytoskeletal & focal adhesion proteins through scanning electron microscopy and immunofluorescence staining. Increased cell spreading and proliferation rates are observed on surfaces with 50 nm thick Ti coatings. The micro-groove geometries have been shown to promote contact guidance, which leads to reduced scar tissue formation. In contrast, smooth surfaces result in random cell orientations and the increased possibility of scar tissue formation. Immunofluorescence cell staining experiments also reveal that the actin stress fibers are aligned along the groove dimensions, with discrete focal adhesions occurring along the ridges, within the grooves and at the ends of the cell extensions. The implications of the observed cell/surface interactions are discussed for possible applications of silicon in implantable biomedical systems.
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References
J. W. GARDNER, V. K. VARADAN and O. O. AWADELKARIM, “Microsensors, MEMS, and Smart Devices” (Wiley, New York, 2002).
B. ZIAIE and K. NAJAFI, Biomed. Micr. 3(4) (2001) 285.
D. LIEPMANN, in Proceedings of The 3rd Korean MEMS Conference (Seoul, 2001) p. 13.
BABAK ZIAIE X. CAO, S. LAI and L. LEE, Biomed. Micr. 3(2) (2001) 109.
Z. STRONG, A. WANG and C. MCCONAGHY, Biomed. Microdevices 4(2) (2002) 97.
G. VOSKERICIAN, M. S. SHIVE, R. S. SHAWGO, H. VON RECUM, J. M. ANDERSON, M. J. CIMA and R. LANGER, Biomater. 24 (2003) 1959.
B. D. RATNER, A. S. HOFFMAN, F. J. SCHOEN and J. E. LEMONS, “Biomaterials Science: An Introduction to Materials in Medicine” (Academic Press, San Diego, 1996).
Y. HANEIN, Y. V. PAN, B. D. RATNER, D. DENTON and K. F. BÖHRINGER, Sens. Act. B: Chem. 81(1) (2001) 49.
C. MILBURN, S. MWENIFUMBO, E. KUNG and W. O. SOBOYEJO, Effects of biocompatible nano-scale titanium coatings on cell spreading and adhesion to biomems structures, to be submitted.
D. M. BRUNETTE, “Titanium in Medicine: Material Science, Surface Science, Engineering, Biological Responses and Medical Applications” (Springer-Verlag, New York, 2001).
W. O. SOBOYEJO, B. NEMETSKI, S. ALLAMEH, N. MARCANTONIO, C. MERCER and J. RICCI, J. Biomed. Mater. Res. 62 (2002) 56.
W. O. SOBOYEJO, C. MERCER, S. ALLAMEH, B. NEMETSKI, N. MARCANTONIO and J. L. RICCI, Key Eng. Mater. 198–199 (2001) 203.
K. ANSELME, P. LINEZ, M. BIGERELLE, D. LE MAGUER, A. LE MAGUER, P. HARDOUIN, H. F. HILDEBRAND, A. IOST and J. M. LEROY, Biomater. 21 (2000) 1567.
K. ANSELME, M. BIGERELLE, B. NOEL, E. DUFRESNE, D. JUDAS, A. IOST and P. HARDOUIN Biomed. Mater. Res. 49 (2000) 155.
K. ANSELME, B. NOEL and P. HARDOUN, J. Mater. Sci.: Mater. Med. 10 (1999) 815.
J. L. RICCI and H. ALEXANDER, Key Eng. Mater. 198–199 (2001) 179.
J. L. RICCI, J. CHARVET, S. R. FRENKEL, R. CHANG, P. NADKARNI, J. TURNER and H. ALEXANDER, “Bone response to laser microtextured surfaces”, Bone Engineering, edited by J. E. Davies, (Em2 Inc., Toronto, 2000) p. 1.
J. H-C WANG, E. S. GROOD, J. FLORER and R. WENSTRUP, J. Biomech. 33 (2000) 729.
J. H.-C. WANG and E.S. GROOD, Connective Tissue Res. 41 (2000) 29.
K. D. CHESMAL and J. BLACK, J. Biomed. Mater. Res. 29 (1995) 1089.
K. D. CHESMAL, C. C. CLARK, C. T. BRIGHTON and J. BLACK, J. Biomed. Mater. Res. 29 (1995) 1101.
E. DEN BRABER, J. DE RUIJTER, H. SMITS, L. GINSEL, A. F. VON RECUM and J. A. JANSEN, J. Biomed. Mater. Res. 29 (1995) 511.
J. L. RICCI, J. CHARVET and R. CHANG, in Proceedings of 20th Annual Meeting of the Society for Biomaterials (Boston, 1994) p. 401.
P. CLARK, D. COLES and M. PECKHAM, Exp. Cell Res. 230 (1997) 275.
R. F. WOOD, C. W. WHITE and R. T. YOUNG, “Semiconductors and Semimetals 23” (Academic, Orlando, 1984).
M. MOSBACHER, H. J. MUNZER, M. BERTSCH, V. DOBLER, N. CHAOUI, J. SIEGEL, R. OLTRA, D. BAUERLE, J. BONEBERG and P. LEIDERER, “Laser assisted Particle Removal from Silicon Wafers”, Particles on Surfaces: Detection, Adhesion and Removal 7, (VSP International Science Publisher, Netherlands, 2002), p. 275.
A. G. CULLIS, Reports Prog. Phys 48 (1985) 1155.
S. VERCAIGNE, J. WOLKE, I. NAERT and J. JANSEN, J. Biomed. Mater. Res. 41 (1998) 41.
M. LI, N. MORRIS, S. MWENIFUMBO, M. KEIRSTEAD and W. SOBOYEJO, in Proceedings of SPIE 4830 (Osaka, 2002) 206.
S. MWENIFUMBO, N. D. MORRIS, M. LI and W. O. SOBOYEJO, “Surface Engineering in Materials Science II” (San Diego, 2003) p. 141.
A. M. P. TURNER, N. DOWELL, S. W. P. TURNER, L. KAM, M. ISAACSON, J. N. TURNER, H. G. CRAIGHEAD and W. SHAIN, J. Biomed. Mater. Res. 51 (2000) 430.
N. D. SPENCER and M. TEXTOR, Surface Modification, Surface Analysis and Biomaterials. Materials in Medicine, edited by MD, Speidel and P Uggowitzer, (ETHZ, 1998).
B. FENG, J. Y. CHEN, S. K. QI, L. HE, J. Z. ZHAO and X. D. ZHANG, J. Mater. Sci.: Mater. Med. 13 (2002) 457.
B. FENG, J. WENG, B. C. YANG, J. Y. CHEN, J. Z. ZHAO, L. HE, S. K. QI and X. D. ZHANG Mater. Character., 49 (2003) 129.
M. E. CHICUREL, C. S. CHEN and D. E. INGBER, Curr. Opin. Cell Biol. 10 (1998) 232.
E. RUOSLAHTI and B. OBRINK, Exp. Cell Res. 227 (1996) 1.
K. ANSELME, Biomater. 21 (2000) 667.
P. CLARK, P. CONNOLLY, A. CURTIS, J. DOW and C. WILKINSON, Development 99 (1987) 439.
A. CURTIS and C. WILKINSON, Biomater. 18 (1997) 1573–1583.
X. F. WALBOOMERS, W. MONAGHAN, A. CURTIS and J. A. JANSEN, J. Biomed. Mater. Res. 46 (1999) 212.
P. CLARK, P. CONNOLLY, A. CURTIS, J. DOW and C. WILKINSON, Development 108 (1990) 635.
A. CURTIS and P. CLARK, CRC Rev. Biocomp. 5 (1990) 343.
J. MEYLE, K. GUTLIG and W. NISCH, J. Biomed. Mater. Res. 29 (1995) 81.
A. GREEN, J. JANSEN, J. VAN DER WAERDEN and A. VON RECUM, J. Biomed. Mater. Res. 28 (1994) 647.
H. G. CRAIGHEAD, S. W. TURNER, R. C. DAVIS, C. JAMES, A. M. PEREZ, P. M. ST. JOHN and M.S. ISAACSON. J. Biomed. Dev. 1(1) (1998) 49.
J. A. T. DOW, P. CLARK, P. CONNOLLY, A. S. G. CURTIS and C. D. W. WILKINSON, J. Cell. Sci. Suppl. 8 (1987) 55.
S. MWENIFUMBO, Cell/surface interactions and adhesion on biomedical and biomems surfaces: effects of laser micro-texturing and titanium coatings, A thesis of Princeton University (2004) p. 41.
D. WANG, K. CHRISTENSEN, K. CHAWLA, G. XIAO, P. H. KREBSBACH and R. T. FRANCESCHI, J. Bone. Miner. Res. 14(6) (1999) 893.
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Mwenifumbo, S., Li, M., Chen, J. et al. Cell/surface interactions on laser micro-textured titanium-coated silicon surfaces. J Mater Sci: Mater Med 18, 9–23 (2007). https://doi.org/10.1007/s10856-006-0658-9
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DOI: https://doi.org/10.1007/s10856-006-0658-9