Abstract
Photoacoustic microscopy (PAM) has been shown to be a suitable technique to obtain thermal images of a wide variety of samples from semiconductors to biological material. In PAM, the incidence of a modulated laser beam on a sample within a photoacoustic (PA) cell, hermetically sealed, produces a PA signal which depends on the thermal and optical properties of the studied sample. By making a sweep of the modulated laser beam on the sample surface, it is possible to obtain the PA signal as a function of their x−y coordinates, and from this signal, it is possible to reconstruct thermal images of the sample. In this study, thermal images of a coffee-seed germ were obtained, with a difference of 12 h between them, by using the PAM technique. Thermal differences observed between images give information which reflects degradation due to the fact that germ cells undergo changes as a function of time. The thermal images obtained by the PAM technique could be applied to biological materials that have a complex constitution (not homogeneous) in their structures, and thermal differences can be observed. PAM is a non-destructive technique, which is an important feature for this type of study. Other applications of this technique can be performed in the agricultural and biotechnological areas.
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Mestres P., Petrov D.: Eur. Biophys. J. 40, 1081 (2011). doi:10.1007/s00249-011-0725-0
Goentoro L., Shoval O., Kirschner M.W., Alon U.: Mol. Cell 36, 894 (2009)
Rosencwaig A., Gersho A.: J. Appl. Phys. 47, 64 (1976)
I.F. Faria Jr., C.C. Ghizoni, L.C.M. Miranda, Appl. Phys. Lett. 47(1985) 1154
Marinelli M., Murtas F., Mecozzi M.G., Zammit U., Pizzoferrato R., Scudieri F., Martellucci S., Marinelli M.: Appl. Phys. A 51, 387 (1990). doi:10.1007/BF00348378
Li Voti R., Sibilia C., Bertolotti M.: Int. J. Thermophys. 26, 1833 (2005)
Mandelis A., Feng C.: Phys. Rev. E 65, 021909 (2002)
Vargas H., Miranda L.C.M.: Phys. Rep. 161, 43 (1988)
Busse G.: Appl. Opt. 21, 107 (1982)
Domínguez Pacheco A., Hernández Aguilar C., Cruz Orea A., Briseño-Tepepa B.R., Sánchez Sinéncio F., Martínez Ortíz E., Valcarcel J.P.: Int. J. Thermophys. 30, 2036 (2009)
A. Dominguez Pacheco, C. Hernandez Aguilar, A. Cruz-Orea, E. Martinez Ortiz, E. Ayala-Maycotte, J. Phys. Conf. Ser. 214, 012060 (2010)
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Domínguez-Pacheco, A., Hernández Aguilar, C., Cruz-Orea, A. et al. Thermal Image of Coffee-Seed Germ Obtained by Photoacoustic Microscopy. Int J Thermophys 34, 1499–1503 (2013). https://doi.org/10.1007/s10765-012-1331-9
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DOI: https://doi.org/10.1007/s10765-012-1331-9