Abstract
We examined the interaction between environmental variables measured at three different scales (i.e., landscape, lake, and in-lake) and fish assemblage descriptors across a range of over 50 floodplain lakes in the Mississippi Alluvial Valley of Mississippi and Arkansas. Our goal was to identify important local- and landscape-level determinants of fish assemblage structure. Relationships between fish assemblage structure and variables measured at broader scales (i.e., landscape-level and lake-level) were hypothesized to be stronger than relationships with variables measured at finer scales (i.e., in-lake variables). Results suggest that fish assemblage structure in floodplain lakes was influenced by variables operating on three different scales. However, and contrary to expectations, canonical correlations between in-lake environmental characteristics and fish assemblage structure were generally stronger than correlations between landscape-level and lake-level variables and fish assemblage structure, suggesting a hierarchy of influence. From a resource management perspective, our study suggests that landscape-level and lake-level variables may be manipulated for conservation or restoration purposes, and in-lake variables and fish assemblage structure may be used to monitor the success of such efforts.
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Introduction
Floodplain lakes created by the meandering of rivers are dynamic systems with unique and diverse habitats and environmental conditions (Baker et al., 1991; Sabo & Kelso, 1991). Thus, floodplain lakes are excellent systems for examining relationships between abiotic and biotic factors and fish assemblage structure. Floodplain lakes and other extra-channel habitats are important components of river–floodplain ecosystems in view of the fact that floodplain habitats support reproduction, growth, and recruitment of many channel-dwelling fishes (Welcomme, 1979; Penczak et al., 2003; Zeug et al., 2005; Nunn et al., 2007). Moreover, floodplain lakes support their own unique fish assemblages and species. Thus, there is a direct relationship between fish assemblage characteristics in floodplain habitats and fish assemblage characteristics in the main channel (Welcomme, 1979), and a river system’s gamma diversity. Recognition of these important linkages and identification of variables influencing fish assemblage structure in floodplain lakes can aid in conservation, restoration, and management of floodplain lakes and their respective river systems.
Previous studies have identified a variety of environmental variables as determinants of floodplain lake fish assemblages (e.g., Winemiller et al., 2000; Miranda & Lucas, 2004; Penczak et al., 2004; Tales & Berrebi, 2007). Environmental variables that determine floodplain lake fish biodiversity (e.g., richness, diversity, and evenness metrics) and assemblage composition (e.g., taxa and functional guilds) frequently originate from multiple spatial scales (e.g., Allen & Starr, 1982; Imhof et al., 1996; Dembkowski & Miranda, 2012). Relative importance of deterministic variables may change as a function of spatial scale, suggesting that fish assemblage determinants at a given scale may be the result of processes occurring at other, usually larger, spatial scales (i.e., show a hierarchy of influence; Syms, 1995; Brind’Amour et al., 2005). For example, a study may identify variables at the local and landscape-level scales as deterministic of fish assemblage structure, when in fact the local variables may be mediated by the landscape-level variables. Understanding the influence of fish assemblage determinants functioning on multiple spatial scales can foster the development of a more efficient and holistic approach to floodplain ecosystem conservation and restoration. Conditional upon a hierarchy of influence, conservation and management efforts can be focused on landscape-level variables, which mediate in-lake variables, which ultimately mediate fish assemblage structure.
We examined the interactions between three classes of environmental variables (i.e., landscape, lake, and in-lake levels) and fish assemblage structure across a range of floodplain lakes in the Mississippi Alluvial Valley of Mississippi and Arkansas. Each class of variables fell onto a gradient of resolution where landscape-level variables represented the coarsest resolution (i.e., broadest scale) and in-lake variables represented the finest resolution. Variables measured on a broader scale (e.g., landscape- and lake-level variables) have been identified by other authors as deterministic of variables measured on a finer scale, such as in-lake processes, biodiversity, and fish assemblage composition in standing bodies of water (e.g., Lucas, 1985; Magnuson et al., 1998; Tejerina-Garro et al., 1998; Dembkowski & Miranda, 2012). The specific objective of our study was to identify important local- and landscape-level determinants of fish assemblage structure in floodplain lakes. Also of interest were relationships between landscape, lake, and in-lake descriptors and frequency of occurrence of selected taxonomic and feeding guilds. Relationships between fish assemblage structure and variables measured at broader scales (i.e., landscape- and lake-levels) were expected to be stronger than relationships between fish assemblage structure and variables measured at finer scales (i.e., in-lake variables). We also hypothesized strong relationships among the three classes of environmental variables based on previous hierarchical theory (e.g., Allen & Starr, 1982).
Methods
Site description
The alluvial valley of the lower Mississippi River extends from near Cairo, Illinois south to the confluence of the Mississippi and Atchafalaya rivers in east-central Louisiana (Fig. 1; Killgore et al., 2007). Within the states of Mississippi and Arkansas, major tributary streams to the Mississippi River include the Yazoo, White, Arkansas, and Ouachita rivers, each with extensive tributary networks. Hundreds of floodplain lakes are situated along these tributary streams. Most of the lakes are oxbows, resulting from river channel abandonment due to sediment deposition or anthropogenic channel alterations (Biedenharn et al., 2000). Fifty-three floodplain lakes within the Yazoo, White, Arkansas, and Ouachita River basins were investigated from July 2006 to August 2010 (Fig. 1). These lakes were channel remnants of the extant or ancient rivers that flow or had once flowed through the valley. Although there are many floodplain lakes in the region that are channel remnants of the present Mississippi River, those lakes are larger and were not considered for this study. Forty lakes were situated adjacent to the Yazoo River and its major tributaries (the Coldwater, Sunflower, Yalobusha, and Tallahatchie rivers) and 13 lakes were within the Arkansas, White, and Ouachita River basins. Eight lakes from the White River Basin were located within the White River National Wildlife Refuge, Arkansas. Three lakes from the Yazoo River Basin were located within the Delta National Forest, Mississippi. Lakes were selected based on diverse representation of landscape and basin characteristics, and accessibility. Efforts were made to select lakes along perceived gradients of environmental variables including depth, surface area, degree of connectivity with the closest rivers, and riparian and watershed land-cover compositions.
Landscape variables
Landscape-level variables selected for analyses included descriptors of riparian and watershed land-covers. Lake-specific watersheds could not be defined because of the lack of sufficient topographic relief in the region (Baker et al., 1991). Instead, concentric bands (50-, 500-, 1,000-, and 5,000-m) were drawn around each lake. The 50-m band was designated as the riparian zone, and the 500-, 1,000-, and 5,000-m bands were considered descriptive of the encircling land-cover and a proxy to a lake’s watershed. Land-cover data (queried in 2009 and 2010) within each band was obtained from the National Agriculture Imagery Program (NAIP), Mississippi Automated Resource Information System (MARIS, 2011), Arkansas GeoStor (2009), and the U.S. Geological Survey (USGS) Southeast Gap Analysis Project (SEGAP) databases. Land-cover classes identified within the riparian zone consisted of row-crop agriculture, forest cover, wetlands, and human disturbance (e.g., urban development and impervious surfaces). Land-cover classes identified within the broader concentric bands were the same as those of the riparian zone, although a freshwater class that included other water bodies was also included. Percentages of the land-cover classes were calculated within each concentric band. All geospatial data were extracted with spatial analyst tools available in the Arc-GIS software package. Lakes were treated as polygons, and those that had not already been identified as water bodies in Arc-GIS were digitized and added.
Lake variables
Floodplain lake-main channel interconnectedness, maximum depth, and surface area were selected for analyses on the basis that these variables are often identified as fundamental to many other processes in floodplain dynamics (Junk et al., 1989; Baker et al., 1991; Miranda, 2005; Lubinski et al., 2008). In addition, Dembkowski & Miranda (2012) demonstrated the hierarchical influence of connectivity, depth, and surface area on fish biodiversity in a similar set of floodplain lakes in the Mississippi Alluvial Valley and, because biodiversity is an integral component of assemblage structure, these variables are thought to affect fish assemblage structure in a similar manner.
The degree of connectivity between floodplain lakes and the nearest river is likely influenced by the linear distance between the connection points, the change in elevation from the floodplain lake to the river channel, and anthropogenic modifications such as levees and channelization. Several methods of measuring lake–river interconnectedness have been developed, including counts of inlets/outlets and area of neighboring water bodies (Miyazono et al., 2010), qualitative indices (Miranda, 2005; Lubinski et al., 2008), and comparisons of direct field observations of flooding with river discharge levels (Zeug et al., 2005). Direct observation of connection events and comparison with river discharge levels is likely the most precise index of connectivity; however, direct observation of flooding at all the studied lakes included in the present research was impractical. Also, available elevation data in the study regions were of relatively low resolution. Furthermore, agricultural practices, leveeing, and ditching have altered the hydrology of the study region to the point where elevation data have limited utility. Because of the general unreliability of other connectivity indices and the limited utility of elevation data, we used effective distance, defined as channel distance between each lake and the nearest river, as an index of lake–river interconnectedness. The channels were sinuous ephemeral streams connecting each floodplain lake to the nearest river. Effective distance is easily measured and should index connectivity as the lakes closer to the nearest rivers are expectedly connected on a more frequent basis than lakes with farther effective distance.
Maximum depth was defined as the deepest point detected by soundings taken with a handheld (DF2200PX, NorCross Marine,Footnote 1 Orlando, Florida) or boat-mounted (X126 DF Sonar, Lowrance Electronics, Tulsa, Oklahoma) depth finder. The depth finders were operated from a boat navigating in a zig-zag pattern along the former thalweg between the two ends of each lake. Maximum depth was selected over mean depth because it better characterizes the cross-sectional morphology of channel remnants than mean depth (Dembkowski & Miranda, 2012). Lake surface area was estimated using spatial analysis tools available in the Arc-GIS software package.
In-lake variables
In-lake variables selected for this study were grouped into two classes representative of water quality and primary productivity. Turbidity (nephelometric turbidity units; NTU), pH, dissolved oxygen (DO; mg l−1), DO saturation (%), temperature (°C), and water transparency (cm) represented lake water quality. Water quality variables were measured twice at each lake during daytime hours in the summer (June–August) from the epilimnion at a single point near the deepest point in each lake. Turbidity, pH, DO, DO saturation, and temperature were measured in situ using a Eureka Manta multiprobe (Eureka Environmental Engineering, Austin, Texas). Water transparency was measured using a Secchi disk (20 cm diameter).
The concentration of chlorophyll a (fluorescence units; FU) was used to index lake primary productivity. Chlorophyll a was also measured in the summer from the epilimnion at a single point near the deepest point in each lake using an Aquafluor™ handheld fluorometer (Turner Designs, Sunnyvale, California).
Fish collections
Fish were collected during daytime hours by a boat electrofisher equipped with a GPP 7.5 Smith-Root™ pulsator unit (Smith-Root, Inc., Vancouver, Washington). Pulsed DC electricity was cycled at 60 Hz with voltage output adjusted according to the specific conductance of each lake to maintain a constant output of 6–8 A. Individual samples consisted of 0.25 h of continuous electrofishing along indiscriminate shoreline areas. We collected 2–16 samples per lake depending on lake area. Fish were netted from the bow of the boat by two netters equipped with 2.7-m long dip nets with 0.4-cm bar mesh. Fish were identified to species and counted before release near the site of capture. Transects were located far enough apart so that fish released at one site would not influence catches in the adjacent transect. Those species too difficult to identify in the field were preserved in a 10% formalin solution and transported to the lab for positive identification with taxonomic keys (Ross, 2001). Although electrofishing does collect small fishes, it is biased against small-bodied species and juveniles of large-bodied species (Reynolds, 1996; Dolan & Miranda, 2003). Thus, our collections reflected a skewed representation of the fish assemblages, yet were adequate to describe differences across lakes (Miranda, 2011).
Statistical analysis
Fish assemblage structure was represented as fish percentage compositional data of species present in at least 90% of the studied lakes. Removal of species present in less than 10% of the studied lakes may improve the signal-to-noise ratio in a dataset and frequently enhances the detection of relationships between biotic assemblage structure and environmental drivers (Cao et al., 2001; Legendre & Gallagher, 2001; McCune & Grace, 2002; Miranda & Lucas, 2004). Collections of threadfin shad Dorosoma petenense, gizzard shad Dorosoma cepedianum, and western mosquitofish Gambusia affinis did not reflect true abundance because of shad fleeing behavior in response to electrofishing, and mosquitofish insusceptibility to electrofishing. Hence, these species were also excluded from analyses (Miranda, 2011; Dembkowski & Miranda, 2012).
Multivariate sets of landscape (i.e., riparian and watershed land-cover variables) and in-lake variables (i.e., water quality and primary productivity variables) were reduced into smaller sets of unrelated and more easily interpretable univariate variables using principal components analysis (PCA; McCune & Grace, 2002). Before PCA, environmental variables were log-transformed to reduce skewness and standardized (mean = 0; SD = 1) to place all variables in the same scale. Principal component axes with eigenvalues larger than 1 were interpreted and retained for subsequent analyses (Johnson, 1998; McCune & Grace, 2002).
Relationships between and among the environmental variables and fish assemblage structure were examined using canonical analysis of principal coordinates (CAP; Anderson & Willis, 2003). The CAP procedure is a multivariate data reduction technique that identifies axes running through a cloud of data points that have the strongest correlation with an external variable (Anderson & Robinson, 2003). Because the CAP analysis essentially ordinates one data matrix in consideration of another, it is a constrained analysis that uses an a priori hypothesis to construct correlations between sets of variables. The CAP approach to constrained ordination is essentially a three-step process that includes a principal coordinates analysis (PCO), selection of m principal coordinate axes, and an ensuing canonical correlation analysis based on a matrix of explanatory variables. When relating a multivariate matrix to a single variable matrix, the CAP analysis produces a single canonical correlation (δ) representing the strength of the association between the m principal coordinate axes and the explanatory variable (M. J. Anderson, University of Auckland, personal communication). Canonical correlations were not corrected to maintain experimentwise error rates because this would have resulted in significance levels too conservative to draw our interests.
Separate CAP analyses were applied to examine correlations between each level of environmental variables (i.e., landscape, lake, and in-lake) and fish assemblage structure. The CAP analysis sought to find correlations between axes representing most of the variation in the fish assemblage matrix relative to each respective environmental variable. We also examined relationships between specific aspects of the fish assemblage and environmental variables. Fishes were grouped into taxonomic and feeding guilds, and CAP was used to assess relationships between guilds and environmental variables. Specifically, we examined relationships between environmental variables and frequency of occurrence of buffalos, catfishes, gars, and sunfishes because these fish were collected in most studied lakes and were of special interest. Fishes were grouped according to feeding guilds as proposed by Balon (1990), Killgore & Hoover (1992), and Goldstein & Simon (1999) and as implemented by Killgore & Hoover (1992), Miyazono et al. (2010), and Dembkowski & Miranda (2011). Feeding guilds representing less than 5% of the total catch by number were not included in analyses. All analyses between the fish assemblage and external variables were performed with Bray–Curtis dissimilarities calculated from fourth-root-transformed percentage compositional data. The fourth-root transformation was selected because it reduced skewness of the fish assemblage data better than other transformations. All PCA and CAP analyses were performed using the PERMANOVA+ add-on for PRIMER statistical software package (PRIMER-E Ltd, Plymouth, United Kingdom; Clarke & Gorley, 2006). Significance levels for all statistical procedures were designated at α = 0.05. All analyses were conducted with data from different years combined after a permutational multivariate analysis of variance (PERMANOVA; 999 permutations; Anderson, 2001) indicated no significant year effect on assemblage composition (P = 0.32).
Results
Landscape, lake, and in-lake variables
The studied lakes varied greatly in their landscape, lake, and in-lake variables (Table 1). Ranges of landscape- and lake-level variables reflected our efforts to select diverse lakes along gradients of depth, surface area, connectivity, and riparian and watershed land-covers. Maximum depth ranged from 0.5 to 8.6 m, surface area from 0.01 to 5.7 km2, connectivity between floodplain lakes and the nearest river from 0 to 13.5 km, and riparian and watershed land-covers from primarily agriculture and human disturbance to primarily freshwater and bottomland hardwood forest. Water quality and primary productivity variables showed similar variation.
Riparian land-cover variables were reduced to a single principal component axis (hereafter, “riparian PC1”) representative of 63% of the total variance within the dataset. The percentage of agricultural land and human disturbance displayed positive loadings on the axis, whereas the percentage of forested land displayed negative loadings (Table 2). Watershed land-cover variables were reduced into two principal component axes cumulatively representative of 72% of the total variance within the dataset. The first axis (hereafter, “watershed PC1”) explained approximately 53% of the variance; the percentage of forested land displayed positive loadings, and the percentage of agricultural land and human disturbance displayed negative loadings (Table 3). The second axis (hereafter, “watershed PC2”) explained an additional 19% of the total variance; the percentage of freshwater displayed positive loadings and the percentage of wetlands displayed negative loadings. Water quality variables were also reduced into two principal component axes cumulatively representative of 68% of the total variance. The first axis (hereafter, “water quality PC1”) explained 41% of the total variance; negative values were indicative of higher DO, DO percent saturation, pH, and water temperature. The second axis (hereafter, “water quality PC2”) explained an additional 27% of the total variance and represented a gradient of water transparency, where negative values represented greater transparency (Table 4).
Fish collections
In the multiyear sampling period, over 92,300 fish representing 71 species and 19 families were collected during 118 h of electrofishing (Table 5). Excluding shads, the minimum and maximum numbers of species collected across all studied lakes were 11 and 43, respectively. The most common species were bluegill Lepomis macrochirus (34% of the total catch by number), longear sunfish Lepomis megalotis (10%), orangespotted sunfish Lepomis humilis (9%), smallmouth buffalo Ictiobus bubalus (7%), largemouth bass Micropterus salmoides (7%), brook silverside Labidesthes sicculus (5%), and bigmouth buffalo Ictiobus cyprinellus (4%). Among taxonomic guilds, centrarchids (i.e., sunfishes) were collected most frequently (70% of the total catch by number), followed by catostomids (suckers; 11%), cyprinids (minnows; 5%), atherinids (silversides; 5%), lepisosteids (gars; 5%), and sciaenids (drum; 1%). Among feeding guilds, invertivores were collected most frequently (62% of the total catch by number), followed by invertivore–piscivores (18%), invertivore–detritivores (14%), and piscivores (5%).
Fish assemblage–environment relationships
Statistically significant relationships were found among all levels of environmental variables, and between the landscape, lake, and in-lake level variables and fish assemblage structure (Table 6).
Lake surface area and watershed PC2 were significantly correlated with water quality PC1 (δ = 0.39; P = 0.01 and δ = 0.30; P = 0.03, respectively). Lake depth was significantly correlated with water quality PC2 (δ = 0.63; P = 0.001) and chlorophyll a concentration (δ = 0.67; P = 0.001).
Excluding the degree of lake–river interconnectedness, all landscape- and lake-level variables (i.e., depth, surface area, riparian PC1, watershed PC1, and watershed PC2) were significantly correlated with fish assemblage structure (δ range = 0.46–0.79; all P < 0.05; Table 6). All in-lake variables (i.e., water quality PC1, water quality PC2, and chlorophyll a concentration) were also significantly correlated with fish assemblage structure (δ range = 0.74–0.91; all P < 0.05; Table 6).
Relationships between taxonomic guilds, feeding guilds, and environmental variables
The studied lakes displayed various canonical correlations between the selected taxonomic guilds and each level of environmental variables, but no strong patterns relative to the hierarchy of environmental factors were evident (Table 6). Buffalo frequency of occurrence was significantly correlated with riparian PC1 (δ = 0.29; P = 0.03), depth (δ = 0.45; P = 0.004), and water quality PC2 (δ = 0.38; P = 0.02), suggesting that buffalo representation in the fish assemblage generally increases with shallow water, riparian disturbances, and turbidity. Gar frequency of occurrence was significantly correlated with riparian PC1 (δ = 0.34; P = 0.01), watershed PC1 (δ = 0.34; P = 0.01), and water quality PC2 (δ = 0.38; P = 0.02), suggesting that gar representation in the fish assemblage generally increases with watershed and riparian disturbances and turbidity. Sunfish frequency of occurrence was significantly correlated with watershed PC2 (δ = 0.39; P = 0.01), suggesting that sunfish representation in the fish assemblage generally increases in lakes with a greater percentage of freshwater within their watershed. Catfish frequency of occurrence was not significantly correlated with any of the landscape, lake, or in-lake variables.
Although there were significant relationships between feeding guilds (except catfishes) and various landscape, lake, and in-lake variables, the relationships were not consistent (i.e., feeding guilds were not related to only 1 or 2 environmental variables; Table 6). Invertivore–piscivore frequency of occurrence was significantly correlated with riparian PC1 (δ = 0.48; P = 0.001), the degree of lake–river interconnectedness (δ = 0.49; P = 0.004), and water quality PC1 (δ = 0.29; P = 0.04), suggesting that their representation in the fish assemblage generally decreases with lake–river disconnection and low DO, pH, and temperature, but increases with riparian disturbance. Invertivore frequency of occurrence was also significantly correlated with water quality PC1 (δ = 0.43; P = 0.03), suggesting a concurrent decrease in representation with decreasing DO, pH, and water temperature. Invertivore–detritivore frequency of occurrence was significantly correlated with watershed PC2 (δ = 0.32; P = 0.02), depth (δ = 0.46; P = 0.01) and water quality PC2 (δ = 0.34; P = 0.01), suggesting a general increase in representation with depth reductions and low water transparency. Piscivore frequency of occurrence was significantly correlated with the degree of lake–river interconnectedness and water quality PC2 (δ = 0.38; P = 0.02 and δ = 0.36; P = 0.01, respectively), suggesting an increase in representation in the fish assemblage with lake–river disconnection and turbidity.
Discussion
Fish assemblage structure and taxonomic guilds were associated with landscape, lake, and local in-lake environmental characteristics. Fish assemblage structure was correlated with depth, surface area, and riparian and watershed land-covers. Although taxonomic and feeding guilds were correlated with various environmental variables, these canonical correlations showed no consistent patterns relative to a hierarchical relationship with landscape, lake, and in-lake level variables. Results partially support our initial hypotheses in view of the fact that depth and riparian and watershed land-covers were significant determinants of fish assemblage structure. However, the degree of lake–river interconnectedness was not. In addition, we found stronger correlations between in-lake variables and fish assemblage structure than between landscape-level and lake-level variables and fish assemblage structure.
Land-cover and fish assemblage structure
Riparian and watershed land-covers exerted similar influences on water quality, primary productivity, and fish assemblage structure in our studied lakes. The importance of proper riparian and watershed management is well recognized (e.g., Tebo, 1955; Ritchie, 1972; Walser & Bart, 1999). Nonetheless, sedimentation and nutrient loading resulting from poor riparian and watershed practices continues to plague aquatic resources (Shields et al., 2010). Land-cover practices surrounding floodplain lakes may influence fish assemblage structure directly via physiological harm (Ritchie, 1972; Brunton, 1985) or indirectly via habitat or water quality degradation (Lucas, 1985; Cooper & McHenry, 1989; Hall et al., 1999; Roozen et al., 2003).
Lakes with high levels of riparian and watershed disturbances may experience high sedimentation rates (upwards of 7 cm year−1; McHenry et al., 1982; Cooper & McHenry, 1989), which can accelerate reductions in depth. Thus, fish assemblages in lakes with riparian- and watershed-scale disturbances may be subject to unfavorable shifts in fish assemblage characteristics, as seen in extremely shallow lakes (Miranda, 2011). Similarly, the piscivory-transparency-morphometry model (PTM; Rodríguez & Lewis, 1997) may act to structure fish assemblages in lakes subject to large riparian and watershed disturbances, with sight-feeding piscivores eventually replaced by low-visibility tactile-feeders. Alternatively, because of the influence of suspended sediments and high nutrient loads on water quality characteristics, changes in fish assemblage structure relative to riparian and watershed land-covers may be more reflected in tolerance guilds than taxonomic guilds. However, our analysis was not designed to detect these changes. Moreover, watershed disturbances resulting from practices designed to support agriculture (e.g., clear-cutting, leveeing, ditching, channelization, and impoundments) can change hydrology and the water table, thereby affecting patterns in lake water level fluctuations (Foley et al., 2004).
Depth and fish assemblage structure
Depth has previously been recognized as a deterministic factor influencing the abiotic environment and fish assemblages in floodplain lakes. Depth is largely responsible for thermal, chemical, and light stratification and for patterns of water transparency and planktonic photosynthesis dynamics in freshwater lakes (Scheffer, 2004; Nõges, 2009). Thus, fish assemblage structure is likely directly and indirectly influenced by depth and the forces it exerts on water quality and primary productivity, similar to the effects of surrounding land-cover. For example, Miranda (2011) found strong associations between depth and physicochemical characteristics and depth and fish assemblage structure and hypothesized that correlations between fish assemblage structure and physicochemical variables may be totally or partially regulated by depth.
The PTM model (Rodríguez & Lewis, 1997) provides a case where fish assemblage structure may be mediated by depth via water transparency. The PTM model predicts that relative abundance of fishes with low- and high-visibility feeding adaptations should vary predictably as water transparency declines following reductions in depth and subsequent resuspension of sediments (Hamilton & Lewis, 1990; Rodríguez & Lewis, 1997). Shifts in fish assemblage structure in response to changes in water transparency may be exacerbated in lakes experiencing high sedimentation rates, which experience increased lake-shallowing and would eventually be subject to environmental conditions typical of shallow lakes (i.e., increased turbidity and large fluctuations in DO). In addition, depth is often associated with environmental stability, with deeper lakes providing greater stability than shallower lakes (e.g., Zeug et al., 2005; Shoup & Wahl, 2009; Miranda, 2011).
Surface area and fish assemblage structure
Lake surface area was weakly, albeit significantly correlated with fish assemblage structure. Surface area showed the strongest correlation with water quality and no correlation with primary productivity. It is probable that the correlation between surface area and fish assemblage structure is confounded by the combined effects of depth and surrounding land-cover. For example, reductions in surface area are typical of floodplain lakes undergoing depth reduction via increased sedimentation, which is commonly linked with surrounding land-cover (Miranda, 2005; Wren et al., 2008; Shields et al., 2010).
Relationships between lake surface area and fish assemblage structure are more obscure than relationships between surface area and fish biodiversity (e.g., MacArthur & Wilson, 1967; Eadie et al., 1986; Amarasinghe & Welcomme, 2002). In accordance with the species-area relationship, biodiversity scales positively with surface area (MacArthur & Wilson, 1967; Browne, 1981). Greater surface area may be correlated with greater habitat complexity, and a lake with greater habitat heterogeneity can theoretically support more species that are able to exploit all available habitats. Relationships between surface area and biodiversity can likely be extended to fish assemblage structure in view of the fact that larger lakes with more exploitable niche space and more fishes to fill these niche spaces may have different assemblage structure than smaller lakes.
Connectivity and fish assemblage structure
Before major landscape modifications in the region, lake–river connectivity was an extremely influential component of floodplain ecosystem dynamics (Junk et al., 1989; Baker et al., 1991). In addition, previous studies have demonstrated that connectivity is a driving force behind floodplain lake fish assemblage structure (Miranda, 2005; Zeug et al., 2005; Shoup & Wahl, 2009; Miyazono et al., 2010). Thus, we expected significant correlations between fish assemblage structure and the degree of lake–river interconnectedness. Nonetheless, connectivity was not correlated with fish assemblage structure as a whole.
It is probable that any actual relationships between fish assemblage structure and the degree of lake–river interconnectedness were diluted by the coarseness of our method of measuring connectivity. Dembkowski & Miranda (2012) found no association between floodplain lake fish biodiversity and the degree of lake–river interconnectedness in a similar set of floodplain lakes and hypothesized that the effects of connectivity may influence fish assemblage structure more than fish biodiversity. There is clearly scope for further research in understanding the effects of lake–river interconnectedness on fish assemblage structure in floodplain lakes, as well as in the development of a more accurate measure of lake–river connectivity, especially in regions with minimal topographic relief where leveeing, ditching, and channelization of nearby rivers might have altered natural connection routes. An ideal index of lake–river interconnectedness should probably include not only linear distance of the connection channel, but also physical attributes of the connection channels (e.g., width, depth, and presence of fish passage barriers).
Water quality, primary productivity, and fish assemblage structure
Water quality and primary productivity showed stronger relationships with fish assemblage structure than any of the landscape-level or lake-level variables we considered. Although we expected stronger correlations between landscape- and lake-level variables and fish assemblage structure, the observed trend is not surprising given that local abiotic conditions are a filter operating on the origin and maintenance of fish assemblages as proposed by Tonn (1990) and modified by Tonn et al. (1990) and Wootton (1992). Whereas most of the landscape- and lake-level variables influence fish assemblage structure through direct or indirect pathways, water quality and primary productivity variables likely function to structure fish assemblages directly via physiological tolerance or thresholds. For example, fish assemblages in extremely turbid lakes may be dominated by tactile-feeding species because of the inability of sight-feeding piscivores to survive. Aside from turbidity, variables such as water temperature (Magnuson et al., 1979; Shuter et al., 1980), DO (Zalewski & Naiman, 1984), nutrient loading (Ryder, 1982; Rempel & Colby, 1991), and their diel and seasonal fluxes affect certain fish physiological attributes and have been demonstrated to affect assemblage structure. It should also be noted that fish assemblages themselves may influence water quality and primary productivity (e.g., bioturbation from foraging activities of benthic fishes; Scheffer, 2004; Mormul et al., 2012), but the potential for fish assemblages to influence environmental variables was not investigated in this study. Although water quality and primary productivity influence fish assemblage structure through direct pathways, it is important to note that they are both influenced to a certain degree by landscape-level and lake-level variables. Results of this study suggest that water quality was influenced by lake surface area, watershed land-cover, and depth, and that primary productivity was influenced by depth, but future research should be directed at further-elucidating these relationships to grasp a better understanding of the influence of landscape-level and lake-level variables on in-lake processes.
Ecological applications
Results of this study demonstrate that fish assemblage structure in floodplain lakes of the Mississippi Alluvial Valley was influenced by variables operating on three different scales, but in-lake variables showed stronger relationships with fish assemblage structure than did landscape-level or lake-level variables, suggesting a hierarchy of influence. Thus, in theory, depth, surface area, and riparian and watershed land-covers can be manipulated for conservation and (or) restoration purposes and water quality and primary productivity variables and fish assemblage structure can be used to monitor the success of such efforts. However, lake depth appears to be a “common denominator” for all landscape-level and lake-level variables because lakes subject to losses in surface area and to riparian- and watershed-scale disturbances exhibit abiotic characteristics and shifts in fish assemblage structure similar to lakes undergoing depth reductions. Deeper lakes provide greater environmental stability and may attenuate the negative effects of surface area reduction and sedimentation resulting from riparian and watershed disturbances.
Notes
The use of trade, product, industry, or firm names or products or software is for informative purposes and does not constitute an endorsement by the U.S. government or U.S. Geological Survey.
References
Allen, T. F. H. & T. B. Starr, 1982. Hierarchy: Perspectives for Ecological Complexity. The University of Chicago Press, Chicago, IL.
Amarasinghe, U. S. & R. L. Welcomme, 2002. An analysis of fish species richness in natural lakes. Environmental Biology of Fishes 65: 327–339.
Anderson, M. J., 2001. A new method for non-parametric multivariate analysis of variance. Austral Ecology 26: 32–46.
Anderson, M. J. & J. Robinson, 2003. Generalized discriminant analysis based on distances. Australian and New Zealand Journal of Statistics 45: 301–318.
Anderson, M. J. & T. J. Willis, 2003. Canonical analysis of principal coordinates: a useful method of constrained ordination for ecology. Ecology 84: 511–525.
Baker, J. A., K. J. Killgore & R. L. Kasul, 1991. Aquatic habitats and fish communities in the Lower Mississippi River. Aquatic Sciences 3: 313–356.
Balon, E. K., 1990. Epigenesis of an epigeneticist: the development of some alternative concepts on the early ontogeny and evolution of fishes. Guelph Ichthyology Reviews 1: 1–42.
Biedenharn, D. S., C. R. Thorne & C. C. Watson, 2000. Recent morphological evolution of the lower Mississippi River. Geomorphology 34: 227–249.
Brind’Amour, A., D. Boisclair, P. Legendre & D. Borcard, 2005. Multiscale spatial distribution of a littoral fish community in relation to environmental variables. Limnology and Oceanography 50: 465–479.
Browne, R. A., 1981. Lakes as islands: biogeographic distribution, turnover rates, and species composition in the lakes of central New York. Journal of Biogeography 8: 75–83.
Brunton, M. N., 1985. The effects of suspensoids on fish. Hydrobiologia 125: 221–241.
Cao, Y., D. P. Larsen & R. S. Thorne, 2001. Rare species in multivariate analysis for bioassessment: some considerations. Journal of the North American Benthological Society 20: 144–153.
Clarke, K. R. & R. N. Gorley, 2006. PRIMER v6: User Manual/Tutorial. PRIMER-E, Plymouth, UK.
Cooper, C. M. & J. R. McHenry, 1989. Sediment accumulation and its effects on a Mississippi River oxbow lake. Environmental Geology and Water Sciences 13: 33–37.
Dembkowski, D. J. & L. E. Miranda, 2011. Comparison of fish assemblages in two disjoined segment of an oxbow lake in relation to connectivity. Transactions of the American Fisheries Society 140: 1060–1069.
Dembkowski, D. J. & L. E. Miranda, 2012. Hierarchy in factors affecting fish biodiversity in floodplain lakes of the Mississippi Alluvial Valley. Environmental Biology of Fishes 93: 357–368.
Dolan, C. R. & L. E. Miranda, 2003. Immobilization thresholds of electrofishing relative to fish size. Transactions of the American Fisheries Society 132: 969–977.
Eadie, J. M., T. A. Hurly, R. D. Montgomerie & K. L. Teather, 1986. Lakes and rivers as islands: species–area relationships in the fish faunas of Ontario. Environmental Biology of Fishes 15: 81–89.
Foley, J. A., C. J. Kucharik, T. E. Twine, M. T. Coe & S. D. Donner, 2004. Land use, land cover, and climate change across the Mississippi Basin: impacts on selected land and water resources. In DeFries, R. S., G. P. Asner, & R. A. Houghton (eds), Ecosystems and Land Use Change. Geophysical Monograph Series, Vol. 153. American Geophysical Union, Washington, DC: 249–261.
GeoStor, 2009. Arkansas’ Official GIS Platform. Arkansas Geographic Information Office, Arkansas. http://www.geostor.arkansas.gov/G6/Home.html. Accessed October 2010.
Goldstein, R. M. & T. P. Simon, 1999. Toward a united definition of guild structure for feeding ecology of North American freshwater fishes. In Simon, T. P. (ed.), Assessing the Sustainability and Biological Integrity of Water Resources Using Fish Communities. CRC Press, Boca Raton, FL.
Hall, R. I., P. R. Leavitt, R. Quinlan, A. S. Dixit & J. P. Smol, 1999. Effects of agriculture, urbanization, and climate on water quality in the northern Great Plains. Limnology and Oceanography 44: 739–756.
Hamilton, S. K. & W. H. Lewis, Jr., 1990. Basin morphology in relation to chemical and ecological characteristics of lakes on the Orinoco River floodplain, Venezuela. Hydrobiological Archives 119: 393–425.
Imhof, J. G., J. Fitzgibbon & W. K. Annable, 1996. A hierarchical evaluation system for characterizing watershed ecosystems for fish habitat. Canadian Journal of Fisheries and Aquatic Sciences 53: 312–326.
Johnson, D. E., 1998. Applied Multivariate Methods for Data Analysis. Duxbury Press, Pacific Grove, CA.
Junk, W. J., P. B. Bayley & R. E. Sparks, 1989. The flood pulse concept in river-floodplain systems. In Dodge, D. P. (ed.), Proceedings of the International Large River Symposium. Canadian Special Publication of Fisheries and Aquatic Sciences, Vol. 106. Department of Fisheries and Oceans, Ottawa: 110–127.
Killgore, K. J. & J. P. Hoover, 1992. A Guild for Monitoring and Evaluating Fish Communities in Bottomland Hardwood Wetlands. U.S. Army Corps of Engineers, Waterways Experiment Station, Wetlands Reserve Technical Note FW-EV-2.2, Vicksburg, MS.
Killgore, K. J., J. J. Hoover, J. P. Kirk, S. G. George, B. R. Lewis & C. E. Murphy, 2007. Age and growth of pallid sturgeon in the free-flowing Mississippi River. Journal of Applied Ichthyology 4: 452–456.
Legendre, P. & E. D. Gallagher, 2001. Ecologically meaningful transformations for ordination of species data. Oecologia 129: 271–280.
Lubinski, B. J., J. R. Jackson & M. A. Eggleton, 2008. Relationships between floodplain lake fish communities and environmental variables in a large river-floodplain ecosystem. Transactions of the American Fisheries Society 137: 895–908.
Lucas, G., 1985. Survey of the Fisheries of the Mississippi Delta. Freshwater Fisheries Report 46, Mississippi Department of Wildlife, Fisheries and Parks, Jackson, MS.
MacArthur, R. H. & E. O. Wilson, 1967. The Theory of Island Biogeography. Princeton University Press, Princeton, NJ.
Magnuson, J. J., L. B. Crowder & P. A. Medvick, 1979. Temperature as an ecological resource. American Zoologist 19: 331–343.
Magnuson, J. J., W. M. Tonn, A. Banerjee, J. Toivonen, O. Sanchez & M. Rask, 1998. Isolation vs. extinction in the assembly of fishes in small northern lakes. Ecology 79: 2941–2956.
MARIS (Mississippi Automated Resource Information System), 2003. Mississippi Automated Resource Information System. MARIS, Jackson, MS.
McCune, B. & J. B. Grace, 2002. Analysis of Ecological Communities. MjM Software, Gleneden Beach, OR.
McHenry, J. R., C. M. Cooper & J. C. Ritchie, 1982. Sedimentation of Wolf Lake, lower Yazoo River basin, Mississippi. Journal of Freshwater Ecology 1: 547–558.
Miranda, L. E., 2005. Fish assemblages in oxbow lakes relative to connectivity with the Mississippi River. Transactions of the American Fisheries Society 134: 1480–1489.
Miranda, L. E., 2011. Depth as an organizer of fish assemblages in floodplain lakes. Aquatic Sciences 73: 211–221.
Miranda, L. E. & G. M. Lucas, 2004. Determinism in fish assemblages of floodplain lakes of the vastly disturbed Mississippi Alluvial Valley. Transactions of the American Fisheries Society 133: 358–370.
Miyazono, S., J. N. Aycock, L. E. Miranda & T. E. Tietjen, 2010. Assemblage patterns of fish functional groups relative to habitat connectivity and conditions in floodplain lakes. Ecology of Freshwater Fish 19: 578–585.
Mormul, R. P., S. M. Thomaz, A. A. Agostinho, C. C. Bonecker & N. Mazzeo, 2012. Migratory benthic fishes may induce regime shifts in a tropical floodplain pond. Freshwater Biology 57: 1592–1602.
Nõges, T., 2009. Relationships between morphometry, geographic location and water quality parameters of European lakes. Hydrobiologia 633: 33–43.
Nunn, A. D., J. P. Harvey & I. G. Cowx, 2007. Benefits to 0+ fishes of connecting man-made waterbodies to the lower River Trent, England. River Research and Applications 23: 361–376.
Penczak, T., G. Zieba, H. Kozalinski & A. Kruk, 2003. The importance of oxbow lakes for fish recruitment in a river system. Hydrobiological Archives 158: 267–281.
Penczak, T., W. Galicka, Ł. Glowacki, H. Koszalinski, A. Kruk, G. Zieba, J. Kostrezwa & L. Marszal, 2004. Fish assemblage changes relative to environmental factors and time in the Warta River, Poland, and its oxbow lakes. Journal of Fish Biology 64: 483–501.
Rempel, R. S. & P. J. Colby, 1991. A statistically valid model of the morphoedaphic index. Canadian Journal of Fisheries and Aquatic Sciences 48: 1937–1943.
Reynolds, J. B., 1996. Electrofishing. In Murphy, B. R. & D. W. Willis (eds), Fisheries Techniques, 2nd ed. American Fisheries Society, Bethesda, MD: 221–254.
Ritchie, J. C., 1972. Sediment, fish, and fish habitat. Journal of Soil and Water Conservation 27: 124–125.
Rodríguez, M. A. & W. M. Lewis, Jr., 1997. Structure of fish assemblages along environmental gradients in floodplain lakes of the Orinoco River. Ecological Monographs 67: 109–128.
Roozen, F. M., G. J. van Geest, B. W. Ibelings, R. M. Roijackers, M. Scheffer & A. D. Buijse, 2003. Lake age and water level affect the turbidity of floodplain lakes along the lower Rhine. Freshwater Biology 48: 519–531.
Ross, S. T., 2001. Inland Fishes of Mississippi. University Press of Mississippi, Jackson, MS.
Ryder, R. A., 1982. The morphoedaphic index – use, abuse, and fundamental concepts. Transactions of the American Fisheries Society 111: 154–164.
Sabo, M. J. & W. E. Kelso, 1991. Relationship between morphometry of excavated floodplain ponds along the Mississippi River and their use as fish nurseries. Transactions of the American Fisheries Society 120: 552–561.
Scheffer, M., 2004. The Ecology of Shallow Lakes. Kluwer, Dordrecht.
Shields, F. D., Jr., S. S. Knight, R. E. Lizotte & D. G. Wren, 2010. Floodplain river backwater restoration: a case study. In Proceedings of the 9th Federal Interagency Sedimentation Conference. Joint Federal Interagency Conference Organizing Committee, Washington, DC, CD-ROM.
Shoup, D. E. & D. H. Wahl, 2009. Fish diversity and abundance in relation to interannual and lake-specific variation in abiotic characteristics of floodplain lakes of the Lower Kaskaskia River, Illinois. Transactions of the American Fisheries Society 138: 1076–1092.
Shuter, B. J., J. A. MacLean, F. E. J. Fry & H. A. Regier, 1980. Stochastic simulation of temperature effects on first-year survival of smallmouth bass. Transactions of the American Fisheries Society 109: 1–34.
Syms, C., 1995. Multi-scale analysis of habitat association in a guild of blennioid fishes. Marine Ecology Progress Series 125: 31–43.
Tales, E. & R. Berrebi, 2007. Controls of local young-of-the-year fish species richness in flood plain water bodies: potential effects of habitat heterogeneity, productivity and colonization-extinction events. Ecology of Freshwater Fish 16: 144–154.
Tebo, L. B. Jr., 1955. Effects of siltation, resulting from improper logging, on the bottom fauna of a small trout stream in the southern Appalachians. The Progressive Fish Culturist 17: 64–70.
Tejerina-Garro, F. L., R. Fortin & M. A. Rodríguez, 1998. Fish community structure in relation to environmental variation in floodplain lakes of the Araguaia River, Amazon Basin. Environmental Biology Fish 51: 399–410.
Tonn, W. M., 1990. Climate change and fish communities: a conceptual approach. Transactions of the American Fisheries Society 119: 337–352.
Tonn, W. M., J. J. Magnuson, M. Rask & J. Toivonen, 1990. Intercontinental comparison of small-lake fish assemblages: the balance between local and regional processes. American Naturalist 136: 345–375.
Walser, C. A. & H. L. Bart, Jr., 1999. Influence of agriculture on in-stream habitat and fish community structure in Piedmont watersheds of the Chattahoochie River System. Ecology of Freshwater Fish 8: 237–246.
Welcomme, R. L., 1979. Fisheries Ecology of Floodplain Rivers. Langdon, London: 317 pp.
Winemiller, K. O., S. Tarim, D. Shorman & J. B. Cotner, 2000. Fish assemblage structure in relation to environmental variation among Brazos River oxbow lakes. Transactions of the American Fisheries Society 129: 451–468.
Wootton, R. J., 1992. Fish Ecology. Chapman and Hall, New York.
Wren, D. G., G. R. Davidson, W. G. Walker & S. J. Galicki, 2008. The evolution of an oxbow lake in the Mississippi alluvial floodplain. Journal of Soil and Water Conservation 63: 129–135.
Zalewski, M. & R. J. Naiman, 1984. The regulation of riverine fish communities by a continuum of abiotic–biotic factors. In Alabaster, J. S. (ed.), Habitat Modification and Freshwater Fisheries. Batterworth Scientific, London.
Zeug, S. C., K. O. Winemiller & S. Tarim, 2005. Response of Brazos River oxbow fish assemblages to patterns of hydrologic connectivity and environmental variability. Transactions of the American Fisheries Society 134: 1389–1399.
Acknowledgements
This research was funded by the Vicksburg District of the Army Corps of Engineers through K.J. Killgore, by Mississippi State University, and by the U.S. Geological Survey. The authors thank T. Alfermann, N. Aycock, J. Dagel, R. Krogman, S. Miyazono, and S. Wigen for assistance with fieldwork; and J. Breeggemann, D. Deslauriers, C.A. Hayer, M. Kaemingk, T. Rapp, and M. Wagner for helpful reviews of earlier drafts of the manuscript. (Any use of trade, firm, or product names is for descriptive purposes only and does not constitute endorsement by the U.S. Government).
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Dembkowski, D.J., Miranda, L.E. Environmental variables measured at multiple spatial scales exert uneven influence on fish assemblages of floodplain lakes. Hydrobiologia 721, 129–144 (2014). https://doi.org/10.1007/s10750-013-1655-x
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DOI: https://doi.org/10.1007/s10750-013-1655-x