Abstract
Thailand is a center of diversity for section Angulares of the Asian Vigna (genus Vigna subgenus Ceratotropis) and 4 Vigna species are cultivated in Thailand. Using newly collected wild and cultivated germplasm of Vigna from Thailand and outgroup accessions AFLP analysis was conducted to clarify genetic diversity and relationships. The results suggest that cultivated V. umbellata and V. mungo evolved from wild relatives in a single domestication event. Vigna umbellata is poorly differentiated from its wild and weedy relatives compared to V. mungo. Results suggest northern Thailand and the neighboring Shan state, Myanmar, is the probable center of domestication for V. umbellata as wild accessions from this area and cultivated rice bean from a wide area in Asia are not greatly diverged. Vigna minima, V. tenuicaulis and V. exilis accessions in Thailand are well differentiated with considerable intra-specific variation. Vigna hirtella consists of two well differentiated subgroups, suggesting taxonomic revision may be necessary. Close genetic relationships between V. radiata and V. grandiflora, and between V. mungo and V. trinervia are confirmed. Naturally growing V. mungo populations in northern Thailand appear to be true wild species as they are well differentiated from Indian wild and Thai cultivated populations. The origin of naturally growing cowpea in Thailand needs to be further studied using a more comprehensive set of materials. This study clarifies inter and intra-specific genetic diversity and inter species relationships of Thai Vigna species.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
F.S. Ba R.S. Pasquet P. Gepts (2004) ArticleTitleGenetic diversity in cowpea [Vigna unguiculata (L.) Walp.] as revealed by RAPD markers Genet. Resour. Crop Evol. 51 539–550 Occurrence Handle1:CAS:528:DC%2BD2cXjtFKqt74%3D Occurrence Handle10.1023/B:GRES.0000024158.83190.4e
K.P.S. Chandel R.N. Lester R.J. Starling (1984) ArticleTitleThe wild ancestors of urd and mung beans (Vigna mungo (L.) Hepper and V. radiata (L.) Wilczek) Bot. J. Linn. Soc. 89 85–96
Chavalvut C., Poton N. and Worasan T. 1988. Mungbean and black gram production and development in Thailand. In “Mungbean” Proc. 2nd International Symposium. AVRDC, ShanhuaTaiwan, pp. 657–668.
S. Coulibaly R.S. Pasquet R. Papa P. Gepts (2002) ArticleTitleAFLP analysis of the phenetic organization and genetic diversity of Vigna unguiculata (L.) Walp. reveals extensive gene flow between wild and domesticated types Theor. Appl. Genet. 104 358–366 Occurrence Handle12582708 Occurrence Handle1:CAS:528:DC%2BD38XislOgtrg%3D Occurrence Handle10.1007/s001220100740
S.L. Dellaporta J. Wood J.B. Hicks (1983) ArticleTitleA plant DNA mini preparation: version II Plant Mol. Biol. Rep. 1 19–21 Occurrence Handle1:CAS:528:DyaL2cXksFWhtrk%3D
J. Felsenstein (1992) ArticleTitlePhylogenies from restriction sites, a maximum likelihood approach Evolution 46 557–574 Occurrence Handle10.2307/2409811
J.L. Hamrick M.J.W. Godt (1990) Allozyme diversity in plant species A.H.D. Brown M.T. Clegg A.L. Kahler B.S. Weir (Eds) Plant Population Genetics, Breeding, and Genetic Resources Sinauer Associates Inc Sunderland, MA 43–63
H. Innan R. Terauchi G. Kahl F. Tajima (1999) ArticleTitleA method for estimating nucleotide diversity from AFLP data Genetics 151 1157–1164 Occurrence Handle10049931 Occurrence Handle1:CAS:528:DyaK1MXitVShtb4%3D
A.V. Konarev N. Tomooka D.A. Vaughan (2002) ArticleTitleProteinase inhibitor polymorphism in the genus Vigna subgenus Ceratotropisits biosystematic implications Euphytica 123 165–177 Occurrence Handle1:CAS:528:DC%2BD38Xkt1aks7k%3D Occurrence Handle10.1023/A:1014920309710
Y. Kuroda H. Urairong Y.I. Sato (2003) ArticleTitlePopulation genetic structure of wild rice (Oryza rufipogon) in mainland Southeast Asia as revealed by microsatellite polymorphism Tropics 12 159–170 Occurrence Handle10.3759/tropics.12.159
R. Maréchal J. Mascherpa F. Stainier (1978) ArticleTitleEtude taxonomique d’un groupe complexe d’espèces des genres Phaseolus et Vigna (Palilionaceae) sur la base de données morphologiques et polliniques, traitées par l’analyse informatique Boissiera 28 1–273
M. Nei (1973) ArticleTitleAnalysis of gene diversity in subdivided populations Proc. Natl. Acad. Sci. USA 70 3321–3323 Occurrence Handle4519626 Occurrence Handle1:STN:280:DyaE2c%2FlsFCrtQ%3D%3D Occurrence Handle10.1073/pnas.70.12.3321
N.Q. Ng R. Maréchal (1985) Cowpea taxonomy, origin and germplasm S.R. Singh K.O. Rachie (Eds) Cowpea researchproduction and utilization John Wiley and Sons Chichesterj 11–22
C. Niyomdham (1992) ArticleTitleNotes on Thai and Indo-Chinese Phaseoleae (Leguminosae – Papilionoideae) Nordic J. Bot. 12 339–346 Occurrence Handle10.1111/j.1756-1051.1992.tb01311.x
O. Panaud X. Chen S.R. McCouch (1996) ArticleTitleDevelopment of microsatellite markers and characterization of simple sequence length polymorphism (SSLP) in rice (Oryza sativa L.) Mol. Gen. Genet. 2 252–238
C.E. Shannon W. Weaver (1949) The Mathematical Theory of Communication University of Illinois Press Urbana
P. Srinives C.Y. Yang (1988) Utilization of mungbean germplasm in Thailand Mungbean, Proc. 2nd International Symposium AVRDC ShanhuaTaiwan 71–80
Tateishi Y. 1985. A Revision of the Azuki Bean Group, the Subgenus Ceratotropis of the Genus Vigna (Leguminosae). PhD thesis, Tohoku University.
Y. Tateishi N. Maxted (2002) ArticleTitleNew species and new combinations in Vigna subgenus Ceratotropis (Piper) Verdcourt (LeguminosaePhaseoleae) Kew Bull. 57 625–633
Tomooka N. 1991. Genetic diversity and landrace differentiation of mungbean, Vigna radiata (L.) Wilczek, and evaluation of its wild relatives (the subgenus Ceratotropis) as breeding materials. Tech. Bull. Trop. Agr. Res. CenterJapan. No. 28, pp. 64.
Tomooka N., Abe K., Thein M.S., Twat W., Maw J.B., Vaughan D.A. and Kaga A. 2003. Collaborative exploration and collection of cultivated and wild legume species in Myanmar15th Oct.–15th Nov., 2002. Annual report for Exploration and Introduction of Plant Genetic Resources National Institute of Agrobiological Sciences. 19: 67–83. (in Japanese with English summary).
Tomooka N., Chotechuen S., Boonkerd N., Taengsan B., Nuplean S., Vaughan D.A., Egawa Y., Yokoyama T. and Tateishi Y. 1997. Collection of seed and nodule samples from wild subgenus Ceratotropis species (genus Vigna) in central and northern Thailand. In: Annual Report on Exploration and Introduction of Plant Genetic Resources. Vol. 13. National Institute of Agrobiological Sciences (in Japanese with English summary), pp. 189–206.
N. Tomooka N. Maxted C. Thavarasook A.H.M. Jayasuriya (2002b) ArticleTitleTwo new species, sectional designations and new combinations in Vigna subgenus Ceratotropis (Piper) Verdcourt (Leguminosae) Kew Bull. 57 613–624
Taengsan B., Ornnaichart P. and Egawa Y. 2000. Field survey of high temperature tolerant Asian Vigna species in Thailand. In: Annual Report on Exploration and Introduction of Plant Genetic Resources. Vol. 16. National Institute of Agrobiological Sciences (in Japanese with English summary), pp. 171–186.
N. Tomooka D.A. Vaughan H. Moss N. Maxted (2002a) The Asian Vigna: Genus Vigna subgenus Ceratotropis genetic resources Kluwer Academic Publishers Dordrecht 270
N. Tomooka M.S. Yoon K. Doi A. Kaga D.A. Vaughan (2002c) ArticleTitleAFLP analysis of a Vigna subgenus Ceratotropis core collection Genet. Resour. Crop Evol. 49 521–530 Occurrence Handle10.1023/A:1020954800107
R.Q. Xu N. Tomooka D.A. Vaughan (2000a) ArticleTitleAFLP markers for characterizing the azuki bean (Vigna angularis) complex Crop Sci. 40 808–815 Occurrence Handle1:CAS:528:DC%2BD3cXnsF2mtbw%3D Occurrence Handle10.2135/cropsci2000.403808x
R.Q. Xu N. Tomooka D.A. Vaughan K. Doi (2000b) ArticleTitleThe Vigna angularis complex: genetic variation and relationships revealed by RAPD analysis, and their implications for in situ conservation and domestication Genet. Resour. Crop Evol. 47 123–134 Occurrence Handle10.1023/A:1008769204620
M.S. Yoon K. Doi A. Kaga N. Tomooka D.A. Vaughan (2000) ArticleTitleAnalysis of genetic diversity in the Vigna minima complex and related species in East Asia J. Plant Res. 113 375–386 Occurrence Handle10.1007/PL00013945
X.X. Zong A. Kaga N. Tomooka X.W. Wang O.K. Han D.A. Vaughan (2003) ArticleTitleThe genetic diversity of the Vigna angularis complex in Asia Genome 46 647–658 Occurrence Handle12897872 Occurrence Handle1:CAS:528:DC%2BD3sXnsVert74%3D Occurrence Handle10.1139/g03-041
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Seehalak, W., Tomooka, N., Waranyuwat, A. et al. Genetic Diversity of the Vigna Germplasm from Thailand and Neighboring Regions Revealed by AFLP Analysis. Genet Resour Crop Evol 53, 1043–1059 (2006). https://doi.org/10.1007/s10722-004-7939-2
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10722-004-7939-2