Abstract
Detection of DNA polymorphism in cultivated pigeonpea (Cajanus cajan) and two of its wild relatives Cajanus volubilis and Rhynchosia bracteata is reported here for the first time using amplified fragment length polymorphism (AFLP) fingerprinting. For this purpose, two EcoRI (three selective nucleotides) and 14 MseI (three selective nucleotides) primers were used. The two wild species shared only 7.15% bands with the pigeonpea cultivars, whereas 86.71% common bands were seen among cultivars. Similarly, 62.08% bands were polymorphic between C. volubilis and pigeonpea cultivars in comparison to 63.33% polymorphic bands between R. bracteata and pigeonpea cultivars, and 13.28% polymorphic bands among pigeonpea cultivars. The cluster analysis revealed low polymorphism among pigeonpea cultivars and very high polymorphism between cultivated pigeonpea and its wild relatives. The AFLP analysis also indicated that only one primer combination (EcoRI + ACT and MseI + CTG), at the most any four primer pair combinations, are sufficient for obtaining reliable estimation of genetic diversity in closely related cultivars like pigeonpea material analyzed herein. AFLP analysis may prove to be a useful tool for molecular characterization of pigeonpea cultivars and its wild relatives and for possible use in genome mapping.
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R.K. Aggarwal D.S. Brar S. Nandi N. Huang G.S. Kush (1999) ArticleTitlePhylogenetic relationships among Oryza species revealed by AFLP markers Theor. Appl. Genet. 98 1320–1328 Occurrence Handle10.1007/s001220051198 Occurrence Handle1:CAS:528:DyaK1MXkvVGks78%3D
R.K. Aggarwal V.V. Shenoy J. Ramadevi R. Rajkumar L. Singh (2002) ArticleTitleMolecular characterization of some Indian Basmati and other elite rice genotypes using fluorescent- AFLP Theor. Appl. Genet. 105 680–690 Occurrence Handle1:CAS:528:DC%2BD38XnvVSgsro%3D Occurrence Handle12582481
J. Becker P. Vos M. Kuiper F. Salamini M. Heun (1995) ArticleTitleCombined mapping of AFLP and RFLP marker in barley Mol. Gen. Genet. 249 65–73 Occurrence Handle1:CAS:528:DyaK28Xht1SisrY%3D Occurrence Handle8552035
G. Bensnard A. Tagmount P. Baradat A. Vigouroux A. Berville (2002) ArticleTitleMolecular approach of genetic affinities between wild and ornamental Platanus Euphytica 126 401–412
M. Hill H. Witsenboer M. Zabeau P. Vos R. Kesseli R. Michelmore (1996) ArticleTitlePCR-based fingerprinting using AFLP as a tool for studying genetic relationships in Lactuca Theor. Appl. Genet. 93 1202–1210 Occurrence Handle1:CAS:528:DyaK2sXoslaltw%3D%3D
C.P. Joshi H.T. Nguyen (1993) ArticleTitleRAPD (random amplified polymorphic DNA) analysis based intervarietal genetic relationships among hexaploid wheats Plant Sci. 93 95–103 Occurrence Handle10.1016/0168-9452(93)90038-2 Occurrence Handle1:CAS:528:DyaK2cXitlOgs74%3D
K.P. Kollipara L. Singh T. Hymowitz (1994) ArticleTitleGenetic variation of trypsin and chymotrypsin inhibitors in pigeonpea (Cajanus cajan (L.) Millsp.) and its wild relatives Theor. Appl. Genet. 8 986–983
G. Ladizinsky H. Hamel (1980) ArticleTitleSeed protein profile of pigeonpea (Cajanus cajan) and some Atylosia species Euphytica 29 313–317 Occurrence Handle10.1007/BF00025128 Occurrence Handle1:CAS:528:DyaL3cXlvVarsb4%3D
M. Maheswaran P.K. Subudhi S. Nandi J.C. Xu A. Parco D.C. Yang N. Huang (1997) ArticleTitlePolymorphismdistribution and segregation of AFLP markers in a doubled haploid rice population Theor. Appl. Genet. 94 39–45 Occurrence Handle10.1007/s001220050379 Occurrence Handle1:CAS:528:DyaK2sXhvVerurg%3D
K. Maksem D. Leister J. Peleman M. Zadeau F. Salamini C. Gebhardt (1995) ArticleTitleA high- resolution map of the vicinity of two RI locus on chromosome V of potato based RFLP and AFLP markers Mol. Gen. Genet. 249 74–81
B.G. Nadimpalli R.L. Jarret S.C. Pathak G. Kochert (1992) ArticleTitlePhylogenetic relationships of the pigeonpea (Cajanus cajan) based on nuclear restriction fragment length polymorphism Genome 36 216–223
M. Nei W.H. Li (1979) ArticleTitleMathematical Model for studying genetic variations in terms of restriction endonucleases Proc. Natl. Acad. Sci. USA 76 5269–5273 Occurrence Handle1:CAS:528:DyaL3cXitVWn Occurrence Handle291943
Y.L. Nene V.K. Sheila (1990) Pigeonpea: geography and importance Y.L. Nene S.D. Hall V.K. Sheila (Eds) The Pigeonpea CAB International Wallingford, UK 1–14
I. Paran E. Aftergoot C. Shifriss (1998) ArticleTitleVariation in Capsicum annuum revealed by RAPD and AFLP markers Euphytica 99 167–173 Occurrence Handle10.1023/A:1018301215945 Occurrence Handle1:CAS:528:DyaK1cXit1Kjurw%3D
S. Paul F.N. Wachira W. Powell R. Waugh (1997) ArticleTitleDiversity and genetic differentiation among populations of Indian and Kenyan tea (Camellia sinensis (L.) O. Kuntze) revealed by AFLP markers Theor. Appl. Genet. 94 255–263 Occurrence Handle10.1007/s001220050408 Occurrence Handle1:CAS:528:DyaK2sXhvV2gsbs%3D
C. Polanco M.L. Ruiz (2002) ArticleTitleAFLP analysis of somaclonal variation in Arabidopsis thaliana regenerated plants Plant Sci. 162 817–824 Occurrence Handle10.1016/S0168-9452(02)00029-8 Occurrence Handle1:CAS:528:DC%2BD38Xjtl2iu74%3D
M.B. Ratnaparkhe V.S. Gupta M.R. Ven Murthy P.K. Ranjekar (1995) ArticleTitleGenetic finger printing of pigeonpea (Cajanus cajan (L.) Millsp.) and its wild relatives using RAPD markers Theor. Appl. Genet. 91 893–898 Occurrence Handle10.1007/BF00223897 Occurrence Handle1:CAS:528:DyaK28XmtVCitg%3D%3D
P. Remanandan (1980) The wild gene pool of Cajanus at ICRISAT. Present and future Y.L. Nene (Eds) Procedings of the International workshop Pigeonpea NumberInSeriesVol. 2 ICRISAT Patancheru, A.P., India 29–38
S.O. Rogers A.J. Bendich (1988) Extraction of DNA from plant tissues S.B. Gelvin R.A. Schilperoort (Eds) Plant Molecular Biology Manual, A6: 1 Kluwer Academic Publishers Dordrecht, the Netherlands
K.B. Saxena D. Sharma (1990) Pigeonpea: genetics Y.L. Nene S.D. Hall V.K. Sheila (Eds) The Pigeonpea CAB International Wallingford, UK 137–157
J.W. Schut X. Qi P. Stam (1997) ArticleTitleAssociation between relationship measures based on AFLP markers, pedigree data and morphological traits in barley Theor. Appl. Genet. 95 1161–1168 Occurrence Handle10.1007/s001220050677 Occurrence Handle1:CAS:528:DyaK1cXhsFajuw%3D%3D
H.C. Sharma G. Pampapathy L.J. Reddy (2003) ArticleTitleWild relatives of pigeonpea as a source of resistance to the pod fly (Melanagromyza obtuse Malloch) and pod wasp (Tanaostigmodes cajaninae La Salle) Genet. Resour. Crop Evol. 50 817–824 Occurrence Handle10.1023/A:1025967528757
S.K. Sharma M.R. Knox T.H.N. Ellis (1996) ArticleTitleAFLP analysis of the diversity and phylogeny of Lensits comparison with RAPD analysis Theor. Appl. Genet. 93 751–758 Occurrence Handle10.1007/BF00224072 Occurrence Handle1:CAS:528:DyaK28XntF2mtrY%3D
P.H.A. Sneath R.R. Sokal (Eds) (1973) Numerical Taxonomy: The Principles and Practice of Numerical Classification Freeman & Co San Francisco
P. Vos R. Hogers M. Bleeker M. Reijans T. Lee Particlede M. Hornes A. Frijters J. Pot J. Peleman M. Kuiper M. Zabeau (1995) ArticleTitleAFLP; a new technique for DNA fingerprinting Nucleic Acid Res. 21 4407–4414
C.E. Williams D.A.S. Clair (1993) ArticleTitlePhenotypic relationships and levels of variability detected by restriction fragment length polymorphism and random amplified polymorphic DNA analysis of cultivated and accessions of Lycopersicon esculentum Genome 36 619–630 Occurrence Handle1:CAS:528:DyaK3sXmsVamu7k%3D
J. Zhu M.D. Gale S. Quarrie M.T. Jackson G.J. Bryan (1998) ArticleTitleAFLP markers for the study of rice biodiversity Theor. Appl. Genet. 96 602–611 Occurrence Handle10.1007/s001220050778 Occurrence Handle1:CAS:528:DyaK1cXjs1aqsLY%3D
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Panguluri, S.K., Janaiah, K., Govil, J.N. et al. AFLP Fingerprinting in Pigeonpea (Cajanus cajan (L.) Millsp.) and its Wild Relatives. Genet Resour Crop Evol 53, 523–531 (2006). https://doi.org/10.1007/s10722-004-2031-5
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DOI: https://doi.org/10.1007/s10722-004-2031-5