Abstract
Pre-harvest sprouting of durum wheat (Triticum turgidum L. var durum) reduces commercial grade, although the actual effects on processing quality are controversial. Little is known about the genetics of the dormancy component of pre-harvest sprouting resistance in durum. We studied the segregation of dormancy in 98 recombinant inbred lines from a cross of a relatively non-dormant line, CI13102, with a moderately dormant line, Kyle. The lines and parents were grown in field tests over three years, 1996, 1997 and 1998. Spikes were collected at approximately 20% moisture and stored at −23 ∘C. Hand-threshed grain of the lines was germinated, and number of seeds germinated was counted each day. A germination resistance index was calculated to characterize dormancy. Dormancy appeared to be complexly inherited in this cross. Lines were observed that were significantly (P < 0.05) more dormant than the parents. The lines transgressive for dormancy expressed in different combinations of the three environments, indicating an environmental interaction. DNA of lines and parents was tested with simple sequence repeat primers and AFLPs that were used in quantitative trait loci (QTL) analysis of dormancy. Significant QTLs for dormancy were found, with the most notable being on chromosome 1A, where other QTLs for pre-harvest sprouting resistance have been reported in common wheat.
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References
Anderson, J.A., M.E. Sorrells & S.D. Tanksley, 1993. RFLP analysis of genomic regions associated with resistance to preharvest sprouting in wheat. {Crop Sci} 33: 453–459.
Baker, R.J., 1986. Selection Indices in Plant Breeding. CRC Press, Boca Raton FL, U.S.A.
Canadian Grain Commission, 2003. Official Grain Grading Guide http://grainscanada.gc.ca/Pubs/GGG/2003/04-wheat-2003-e.pdf.
Clarke, J.M., R.M. DePauw, J.G. McLeod & T.N. McCaig, 1994. Variation for preharvest sprouting in durum wheat. {Crop Sci} 34: 1632–1635.
Dexter, J.E. & N.M. Edwards, 1999. The implications of frequently encountered grading factors on the processing quality of durum wheat. http://www.grainscanada.gc.ca/Pubs/confpaper/Dexter/Grading/dgrading-e.htm#abstract.
Dexter, J.E., R.R. Matsuo & J.E. Kruger, 1990. The spaghetti-making quality of commercial durum wheat samples with variable alpha-amylase activity. {Cereal Chem} 67: 405–412.
Dick, J.W., D.E. Walsh & K.A. Gilles, 1974. The effect of field sprouting on the quality of durum wheat. {Cereal Chem} 51: 180–188.
Dyck, P.L., J.S. Noll & E. Czarnecki, 1986. Heritability of RL 4137 type of dormancy in two populations of random lines of spring wheat. {Can J Plant Sci} 66: 855–861.
Gordon, A.G., 1971. The germination resistance test –a new test for measuring germination quality of cereals. {Can J Plant Sci} 51: 181–183.
Gordon, I.L., 1983. Sprouting variability in diverse Triticum spp. germplasms. In: J.E. Kruger & D.E. LaBerge (Eds.), Proceedings of Third International Symposium on Preharvest Sprouting. Westview Press, Boulder, U.S.A., pp. 221–230.
Hare, R.A., B.E. Koth & W.C. Barnes, 1988. Pre-harvest sprouting resistance in Triticum turgidum ssp. durum. In: T.E. Miller & R.M.D. Koebner (Eds.), Proceedings of Seventh International Wheat Genetic Symposium, Institute of Plant Science Research, Cambridge, pp. 221–230.
Knapp, S.J., 2001. Mapping quantitative trait loci. In: R.I. Phillips and I.K. Vasil (Eds.), DNA-Based Markers in Plants. Kluwer Academic Publishers, Netherlands, pp. 59–99.
Knapp, S.J., W.W. Stroup & W.M. Ross, 1985. Exact confidence intervals for heritability on a progeny mean basis. Crop Sci 25: 192–194.
Lander, E.S., P. Green, J. Abrahamson, A. Barlow, M.J. Daly, S.E. Lincoln & L. Newberg, 1987. MAPMAKER: An interactive computer package for constucting primary genetic linkage maps of experimental and natural populations. Genomics 1: 174–181.
Lander, E. & L. Kruglyak, 1995. Genetic dissection of complex traits: Guidelines for interpreting and reporting linkage results. Nat Genet 11: 241–247.
Littell, R.C., G.A. Milliken, W.W. Stroup & R.D. Wolfinger, 1996. SAS System for Mixed Models. SAS Institute Inc., Cary NC, U.S.A.
Lynch, M. & B. Walsh, 1998. Genetics and Analysis of Quantitative Traits. Sinauer Associates, Inc., Sunderland, Mass., U.S.A.
Mares, D.J., 1987. Comparisons of tolerance to pre-harvest sprouting within Triticum species, Triticale, and barley. In: D.J. Mares (Ed.), Proceedings of Fourth International Symposium on Preharvest Sprouting. Westview Press, Boulder, U.S.A., pp. 148–156.
McCaig, T.N. & R.M. DePauw, 1992. Breeding for preharvest sprouting tolerance in white-seed-coat spring wheat. Crop Sci 32: 19–23.
Snape, J.W., A.J. Wright & E. Simpson, 1984. Methods for estimating gene numbers for quantitative characters using doubled haploid lines. Theor Appl Genet 67: 143–148.
Soper, J.F., R.G. Cantrell & J.W. Dick, 1989. Sprouting damage and kernel color relationships in durum wheat. Crop Sci 29: 895–888.
Tinker, N.A. & D.E. Mather, 1995. MQTL: Software for simplified composite interval mapping of QTL in multiple environments. J Quant Trait Loci 1: 2.
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Knox, R.E., Clarke, F.R., Clarke, J.M. et al. Genetic analysis of pre-harvest sprouting in a durum wheat cross. Euphytica 143, 261–264 (2005). https://doi.org/10.1007/s10681-005-7874-0
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DOI: https://doi.org/10.1007/s10681-005-7874-0