Abstract
An unusually small y-type high molecular weight (HMW) glutenin subunit gene from Triticum tauschii was sequenced. This gene, encoded at the Glu-D t 1 locus was designated 12.4t and is the smallest HMW glutenin subunit gene described so far in Triticum species. Oligonucleotide primers based on published sequences of HMW glutenin genes were designed to amplify the encoding region and the central repetitive domain of the gene, which produced fragments of 1.4 and 0.85 kb, respectively. PCR products were cloned and sequenced. The derived amino acid sequence was compared with the amino acid sequences of the HMW glutenin subunits Dy12t, from T. tauschii, and subunits Dy10 and Dy12 of T. aestivum. The amino acid sequence deduced from the nucleotide sequence demonstrated that deletions of hexapeptides and nonapeptides were responsible for the reduction in the size of this HMW glutenin subunit. The estimated molecular weight of the Dy12.4t subunit, calculated on the basis of the deduced amino acid sequence, was 45,228 Daltons. There were also single amino acid differences in the N-, C-terminal and central repetitive domains of this gene in comparison to the three other y-type subunits encoded at the Glu-D1 locus. The Dy12.4t subunit showed the highest similarity to the Dy12 subunit present in the hexaploid wheat Chinese Spring.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Anderson, O.D. & F.C. Greene, 1989 The characterisation and comparative analysis of HMW glutenin genes from genomes A and B of hexaploid bread wheat. Theor Appl Genet 77: 689–700.
Anderson, O.D., F.C. Greene, R.E. Yip, N.G. Halford, P.R. Shewry & J.M. Malpica-Romero, 1989 Nucleotide sequences of the two HMW glutenin genes from the D-genome of a hexaploid bread wheat, T. aestivum L. cv Cheyenne. Nucleic Acids Res 17: 461–462.
Appels, R. & E.S. Lagudah, 1990 Manipulation of chromosomal segments from wild wheat for the improvement of bread wheat. Aust J Plant Physiol 17: 253–266.
Bekes, F., O.D. Anderson, P.W. Gras, R.B. Gupta, A. Tam, C.W. Wrigley & R. Appels, 1994 The contribution to the mixing properties of 1D glutenin subunits expressed in a bacterial system. In: R. Henry & J. Ronalds (Eds.) Proc. Improvement of Cereal Quality by Genetic Engineering, pp. 97–104 Plenum Press, New York.
Bekes, F., L. Tamas, P.W. Gras, P.R. Shewry, O.D. Anderson & R. Appels, 1998 Functional domain within the glutenin subunits of wheat. In: A.E. Slinkard (Ed.) Proc. 9th International Wheat Genetics Symposium, Vol. 4, pp. 136–138 University of Saskatchewan, Canada.
Belton, P.S. 1999 On the elasticity of wheat gluten. J Cereal Sci. 29: 103–107.
Belton, P.S., I.J. Colquhoun, J.M. Field, A. Grant, P.R. Shewry & A.S. Tatham 1994 1H and 2H NMR relaxation studies of high M r subunit of wheat glutenin and comparison with elastin J Cereal Sci 19: 115–121.
Belton, P.S., I.J. Colquhoun, J.M. Field, A. Grant, P.R. Shewry, A.S. Tatham & N. Wellner, 1995 FTIR and NMR studies on the hydration of a high M r subunits of glutenin. Int J Biol Macromol 17: 74–80.
D’Ovidio, R., O.A. Tanzarella & E. Porceddu, 1990 Rapid and efficient detection of genetic polymorphism in wheat through amplification by the polymerase chain reaction. Plant Mol Biol 15: 169–171.
D’Ovidio, R., E. Porceddu & D. Lafiandra, 1994 PCR analysis of genes encoding allelic variants of high molecular weight glutenin subunits at the Glu-D1 locus Theor Appl Genet 88: 175–180.
D’Ovidio, R., S. Masci & E. Porceddu, 1995 Development of a set of oligonucleotide primers specific for genes at the Glu-1 complex loci of wheat. Theor Appl Genet 91: 189–194.
D’Ovidio, R., D. Lafiandra & E. Porceddu, 1996 Identification and molecular characterization of a large insertion within the repetitive domain of a high-molecular-weight glutenin subunit gene from hexaploid wheat. Theor Appl Genet 93: 1048–1053.
Eastwood, R.F., E.S. Lagudah, R. Appels, M. Hannah & J.F. Kollmorgen, 1991 Triticum tauschii: A novel source of resistance to cereal cyst nematode (Heterodera avenae). Aust J Agric. Res. 42: 69–77.
Gianibelli, M.C., O.R. Larroque, F. MacRitchie & C.W. Wrigley 2001a Biochemical, genetic and molecular characterization of wheat glutenin and its component subunits. Cereal Chem 78: 635–646.
Gianibelli, M.C., R.B. Gupta, D. Lafiandra, B. Margiotta & F. MacRitchie 2001b Polymorphism of high M r glutenin subunits in Triticum tauschii: Characterisation by chromatography and electrophoretic methods. J Cereal Sci 33: 39–52.
Gianibelli, M.C. & R. Solomon, 2003 A novel y-type HMW-glutenin subunit (12.4t) present in Triticum tauschii. J Cereal Sci 37: 253–256.
Gupta, R.B. & K.W. Shepherd, 1990 Two-step one-dimensional SDS-PAGE analysis of LMW subunits of glutenin. I. Variation and genetic control of the subunits in hexaploid wheats. Theor Appl Genet 80: 65–74.
Gupta, R.B. & F. MacRitchie, 1991 A rapid one-step one-dimensional SDS-PAGE procedure for analysis of subunit composition of glutenin in wheat. J Cereal Sci 14: 105–109.
Kasarda, D.D. 1989 Glutenin structure in relation to wheat quality. In: Wheat is Unique. In: Y. Pomeranz (ed.) American Association of Cereal Chemists, pp. 277–302, St. Paul, MN.
Kerber, E.R. & P.L. Dyck, 1969 Inheritance in hexaploid wheat of leaf rust resistance and other characters derived from Aegilops squarrosa. Can J Genet Cytol 11: 639–647.
Kihara, H. 1944 Discovery of the DD-analyser, one of the ancestors of Triticum vulgare. Agric Hortic 19: 13–14.
Kihara, H., K. Yamashita & M. Tanaka, 1965 Morphological, physiological, geographical and cytological studies in Aegilops and Triticum collected in Pakistan, Afghanistan, Iran. Results of the Kyoto University Scientific Expedition in 1955. 1: 11–18.
Lafiandra, D., G.F. Tucci, A. Pavoni, T. Turchetta & B. Margiotta, 1997 PCR analysis of x-and y-type genes present at the complex Glu-A1 locus in durum and bread wheat. Theor Appl Genet 94: 235–240.
Lagudah, E.S. & R. Appels, 1993 Wild relatives as a source of new germplasm for cereals improvement. In: B.C. Imrie & J.B. Hacker (eds.) Focused Plant Improvement: Toward Responsible and Sustainable Agriculture. Proceedings of the 10th Australian Plant Breeding Conference, pp. 10–21.
Lagudah, E.S. & G.M. Halloran, 1988 Phylogenetic relationships of Triticum tauschii, the D genome donor to hexaploid wheat. 1. Variation in HMW subunits of glutenin and gliadins. Theor Appl Genet 75: 592–598.
Lagudah, E.S. & G.M. Halloran, 1989 Phylogenetic relationships in Triticum tauschii the D genome donor to hexaploid wheat. 3. Variation in, and the genetics of, seed esterases (Est-5). Theor Appl Genet 77: 851–856.
Mackie, A.M., P.J. Sharp & E.S. Lagudah, 1996 The nucleotide and amino acid sequence of a HMW glutenin gene from T. tauschii and comparison with those from the D genome of bread wheat. J Cereal Sci 24: 73–78.
MacRitchie, F. 1992 Physicochemical properties of wheat proteins in relation to functionality. Advances in Food and Nut Res 36: 1–87.
McFadden, E.S. & E.R. Sears, 1946 The origin of Triticum spelta and its free-threshing hexaploid relatives. J Hered 37: 107–116.
Neuhoff, V., N. Arold, D. Taube & W. Ehrhardt, 1988 Improved staining of proteins in polyacrylamide gels including isoelectric focusing gels with clear background at nanogram sensitivity using Coomassie brilliant blue G-250 and R-250. Electrophoresis 9: 221–233.
Payne, P.I. & G.J. Lawrence, 1983 Catalogue of alleles for the complex gene loci, Glu-A1, Glu-B1 and Glu-D1 which code for the high molecular weight subunits of glutenin in hexaploid wheat. Cereal Res Comm 11: 29–35.
Payne, P.I., L.M. Holt, M.G. Jarvis & E.A. Jackson, 1985 Two-dimensional fractionation of the endosperm proteins of bread wheat (Triticum aestivum): biochemical and genetic studies. Cereal Chem 62: 319–326.
Payne, P.I., M.A. Nightingale, A.F. Krattiger & L.M. Holt, 1987 The relationship between HMW glutenin subunit composition and the bread making quality of British-grown wheat varieties. J Sci Food Agric 40: 51–65.
Reddy, P. & R. Appels, 1993 Analysis of a genomic DNA segment carrying the wheat high molecular weight (HMW) glutenin Bx17 subunit and its use as an RFLP marker. Theor Appl Genet 85: 616–624.
Schachtman, D.P., R. Munns & M.I. Whitecross, 1991 Variation in sodium exclusion and tolerance in Triticum tauschii. Crop Sci 31: 992–997.
Shewry, P.R., N.G. Halford & A.S. Tatham, 1992 The high molecular weight subunits of wheat glutenin. J. Cereal Sci 15: 105–120.
Shewry, P.R., A.S. Tatham & P. Lazzeri, 1997 Biotechnology of wheat quality. J Sci Food Agric 73: 397–406.
Shewry, P.R., N.G. Halford & D. Lafiandra, 2003 Genetics of wheat gluten proteins. Adv. in Genet 49: 111–184.
Tahir, M., A. Pavoni, G.F. Tucci, T. Turchetta & D. Lafiandra, 1996 Detection and characterization of a glutenin subunit with unusual high Mr at the Glu-A1 locus in hexaploid wheat. Theor Appl Genet 92: 654–659.
Tilley, K.A., R.E. Benjamin, K.E. Bagorogoza, B.M. Okot-Kotber, O. Prakash & H. Kwen, 2001 Tyrosine cross-links: Molecular basis of gluten structure and function. J Agric Food Chem 49: 2627–2632.
Thompson, R.D., D. Bartels & N.P. Harberd, 1985 Nucleotide sequence of a gene from chromosome 1D of wheat encoding a HMW-glutenin subunit. Nucleic Acids Res 13: 6833-6846.
William, M.D.H.M., R.J. Pena & A. Mujeeb Kazi, 1993 Seed protein and isozyme variations in Triticum tauschii (Aegilops squarrosa). Theor Appl Genet 87: 257–263.
Wrigley, C.W. 1996 Giant proteins with flour power Nature 381: 738–739.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Hassani, M.E., Gianibelli, M.C., Shariflou, M.R. et al. Molecular structure of a novel y-type HMW glutenin subunit gene present in Triticum tauschii . Euphytica 141, 191–198 (2005). https://doi.org/10.1007/s10681-005-6982-1
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/s10681-005-6982-1