Summary
Morphological characterization of 20 common millet (Panicum miliaceum L., 2n = 4x = 36) cultivars and landraces revealed four distinct clusters which were apparently consistent with the grain colors of black, black and brown, red, yellow, and white. Seed remains of medieval millet, recovered from a 15th century layer (King's Palace, Budapest, Hungary), showed reddish yellow grain color after rehydrating on tissue culture medium that was close to grain color of modern cultivar Omszkoje. aDNA of medieval commom millet was extracted successfully, analyzed and compared to modern common millets by ISSR, SSR, CAP and mtDNA. Analyses of fragments and sequences revealed polymorphism at seven ISSR loci (15 alleles) and at the 5S-18S rDNA locus of mtDNA. CAP analysis of the 5S-18S rDNA fragment revealed no SNPs in the restriction sites of six endonucleases TaqI, BsuRI, HinfI, MboI, AluI and RsaI. Sequence alignments of the restriction fragments RsaI also revealed consensus sequence in the medieval sample compared to a modern variety. An attempted phenotype reconstruction indicated that medieval common millet showed the closest morphological similarity to modern millet cultivar Omszkoje.
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Aufhammer, G. & G. Fischbeck, 1964. Ergebnisse von Gefass- und Feldversuchen mit dem Nachbau keimfahiger Gersten- und Haferkorner aus dem Grundstein des 1832 errichteten. Nürnberger Stadttheates. Z Pflanzenzuchtung 51: 345–378.
Al-Jabani, S.M., M. McClelland, C. Petersen & B.W.S. Sobral, 1994. Phylogenetic analysis of organellar DNA sequences in the Andropogoneae: Saccharinae. Theor Appl Genet 88: 933–944.
Baltensperger, D.D., L.A. Nelson, G.E. Frickel & R.L. Anderson, 1997. Registration of ‘Sunrise’ proso millet. Crop Sci 37: 1380.
Biss, P., J. Freeland, J. Silvertown, K. McConway & P. Lutman, 2003. Successful amplification of ricechloroplast microsatellites from century-old grass samles from the park grass experiment. Plant Mol Biol Rep 21: 249–257.
Bornet, B., C. Muller, F. Paulus & M. Branchard, 2002. Highly informative nature of inter simple sequence repeat (ISSR) sequences amplified using tri- and tetra-nucleotide primers from DNA of cauliflower (Brassica oleracea var. botrytis L.). Genome 45: 890–896.
Cekic, C., N.H. Battey & M.J. Wilkinson, 2001. The potential of ISSR-PCR primer-pair combinations for genetic linkage analysis using the seasonal flowering locus in Fragaria as a model. Theor Appl Genet 103: 540–546.
Chang, K. 1968. Archeology of ancient China. Science 162: 519–526.
Colosi, J.C. & B.A. Schaal, 1997. Wild proso millet (Panicum miliaceum) is genetically variable and distinct from crop varieties of proso millet. Weed Sci 45: 509–518.
Doyle, J.J. & J.L. Doyle, 1990. Isolation of plant DNA from fresh tissue. Focus 12: 13–15.
Geervani, P. & B.O. Eggum, 1989. Nutrient composition and protein quality of minor millets. Plant Foods For Human Nutrition 39: 201–208.
Grausgruber, H., C. Sailer, G. Ghambashidze, L. Bolyos & P. Ruckenbauer, 2004. Genetic variation in agronomic and quantitative traits of ancient wheats. In: J. Vollmann, H. Grausgruber & P. Ruckenbauer (Eds.), Genetic variation for plant breeding, pp. 19-22. In: J. Vollmann, H. Grausgruber & P. Ruckenbauer (Eds.), Genetic variation for plant breeding, pp. 279–283. The 17th Eucarpia General Meeting, 8–11, September, Tulln, Austria, ISBN 3-900962-56-1.
Gyulai, G., Z. Mester, J. Kiss, L. Szemán, L. Heszky & A. Idnurm, 2003. Somaclone breeding of reed canarygrass (Phalaris arundinacea L). Grass Forage Sci 58: 210–215.
Harlan, J.R., 1975. Crops and Man. ASA Crop Science Society of America, Madison, WI.
Ho, P.T., 1969. The loess and the origin of Chinese agriculture. Amer Hist Rev 75: 1–36.
Ho, P.T., 1977. The indigenous origins of Chinese agriculture. In: C.A. Reed (Ed.), Origins of Agriculture, pp. 413–484. Mouton Publ., Paris.
Jaccard, P., 1908. Nouvelles recerches sur la distribution florale. Bull Soc Vaudoise Sci Nat 44: 223–270.
Michelmore, R.W., I. Paran & R.V. Kesseli, 1991. Identification of markers linked to disease-resistance genes by bulked-segregant analysis: A rapid method to detect markers in specific genomic regions by using segregating populations. Proc Natl Acad Sci USA 88: 9828–9832.
M'Ribu, H.K. & K.W. Hilu, 1994. Detection of interspecific and intraspecific variation in Panicum millets through random amplified polymorphic DNA. Theor Appl Genet 88: 412–416.
Murray, M.G. & W.F. Thompson, 1980. Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res 8: 4321–4325.
Nyékhelyi, B.D., 2003. Monumenta Historica Budapestinensia XII. Historical Museum of Budapest, Hungary, pp. 1–102.
OMMI, 2004. National list of varieties. In: K. Neszmélyi (Ed.), National Institute for Agricultural Quality Control, Budapest, Hungary.
Parani, M., K. Rajesh, M. Lakshmi, L. Parducci, A.E. Szmidt & A. Parida, 2001. Specific identification in seven small millet species using polymerase chain reaction – Restriction fragment length polymorphism of trnS-pbsC gene region. Genome 44: 495-499.
Petit, R.J., B. Demesure & S. Dumolin, 1998. cpDNA and mtDNA primers in plants. In: A Karp, P.G. Isaac, & D.S. Ingram (Ed.), Molecular tools for screening biodiversity. pp. 256–261. Chapman and Hall, London, ISBN 0 412 63830 4.
Quinn, R.M., 1999. Kamut: Ancient grain, new cereal. In: J. Janick (Ed.), Perspectives on new crops and new uses. pp. 182–183, ASHS Press, Alexandria, VA.
Ruckenbauer, P. von, 1971. Keimfähiger Winterweizen aus dem Jahre 1877. Beobachtungen und Versuche. pp. 372–386. Inst. f. Pflanzenbau und Pflanzenzuchtung d. Hochschule f. Bodenkultur in Wien.
Scholz, H. & V. Mikolas, 1991. The weedy representatives of proso millet (Panicum miliaceum, Poaceae) in Central Europe. Thaiszia 1: 31–41.
Smith, P.M., 1977. Minor crops. Evolution of crop plants. In: N.W. Simmonds (Ed.), pp. 301–324. Longman, London and New York.
Strand, O.E. & R. Behrens, 1981. Identification of wild proso millet. Minneapolis, NM: Agronomy Fact Sheet No. 35, University of Minnesota. p. 2.
Suh, H.S., J.H. Cho, Y.J. Lee & M.H. Heu, 2000. RAPD variation of 13100 and 17310 year-old carbonized rice. In: B. Gordon (Ed.), 4th International Rice Genetics Symposium, Manilla, Philipines, Oct. 22–27, Manila, Philipines. (http://www.carleton.ca∼bgordon/Rice/paper/suh20.htm).
Walters, T.W., 1989. Historical overview on domesticated plants in China with special emphasis on the Cucurbitaceae. Econ Bot 43: 297–313.
Zietkiewicz, E., A. Rafalski & A. Labuda, 1994. Genome fingerprinting by simple sequence repeat (SSR)-anchored polymerase chain reaction amplification. Genomics. 20: 176–183.
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Lágler, R., Gyulai, G., Humphreys, M. et al. Morphological and molecular analysis of common millet (P. miliaceum) cultivars compared to an aDNA sample from the 15th century (Hungary). Euphytica 146, 77–85 (2005). https://doi.org/10.1007/s10681-005-5814-7
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DOI: https://doi.org/10.1007/s10681-005-5814-7