Abstract
Petri disease of grapevine is primarily caused by Phaeomoniella chlamydospora. This pathogen affects mostly young grapevines, but is also implicated in esca disease of older grapevines. Little is known about the disease cycle of this fungus. Infected propagation material was identified as a major means of dissemination of the pathogen. Recently, the pathogen was also detected from soil in South Africa and airborne conidia have been found in vineyards. The aim of this study was to use a molecular detection technique to test different samples collected from nurseries in South Africa at different nursery stages for the presence of P. chlamydospora. A one-tube nested-PCR technique was optimised for detecting P. chlamydospora in DNA extracted from soil, water, callusing medium and grapevine wood. The one-tube nested-PCR was sensitive enough to detect as little as 1 fg of P. chlamydospora genomic DNA from water and 10 fg from wood, callusing medium and soil. PCR analyses of the different nursery samples revealed the presence of several putative 360 bp P. chlamydospora specific bands. Subsequent sequence analyses and/or restriction enzyme digestions of all 360 bp PCR bands confirmed that all bands were P. chlamydospora specific, except for five bands obtained from callusing media and one from water. Phaeomoniella chlamydospora was positively detected in 25% of rootstock cane sections collected from mother blocks, 42% of rootstock cuttings and 16% of scion cuttings collected during grafting, 40% of water samples collected after pre-storage hydration, 67% of water samples collected during grafting, 8% of callusing medium samples and 17% of soil samples collected from mother blocks. These media can therefore be considered as possible inoculum sources of the pathogen during the nursery stages.
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References
Bertelli E, Mugnai L, Surico G (1998) Presence of Phaeoacremonium chlamydosporum in apparently healthy rooted grapevine cuttings Phytopathologia Mediterranea 37: 79–82
Damm U, Fourie PH (2005) Development of a cost-effective protocol for molecular detection of fungal pathogens in soil South African Journal of Science 101: 135–139
Edwards J, Pascoe IG, Salib S and Laukart N (2003) Phaeomoniella chlamydospora and Phaeoacremonium aleophilum can spread into grapevine canes from trunks of infected mother vines. In: 3rd International Workshop on Grapevine Trunk Diseases (p. 29) Lincoln, New Zealand
Eskalen A, Rooney-Latham SN, Feliciano AJ and Gubler WD (2003) Epiphytic occurrence of esca and Petri disease pathogens on grapevine tissues. In: 3rd International Workshop on Grapevine Trunk Diseases (p. 29) Lincoln, New Zealand
Feliciano AJ, Gubler WD (2001) Histological investigations on infection of grape roots and shoots by Phaeoacremonium spp Phytopathologia Mediterranea 40(Supplement): S387–S393
Ferreira JHS (1998) Phialophora terugsterwing – ‘n Algemene probleem by jong wingerde. Wynboer Tegnies March: T6–T8 (in Afrikaans)
Fourie PH, Halleen F, Groenewald M and Crous P (2000) Black goo decline of grapevine. Wynland Augustus: 93–96
Fourie PH, Halleen F (2001) Diagnose van swamsiektes en hul betrokkenheid by terugsterwing van jong wingerd Wynboer 149: 19–23
Fourie PH, Halleen F, van der Vyver F, Schreuder W (2001) Effect of Trichoderma treatments on the occurrence of decline pathogens in the roots and rootstocks of nursery grapevines Phytopathologia Mediterranea 40: S473–S478
Fourie PH, Halleen F (2002) Investigation on the occurrence of Phaeomoniella chlamydospora in canes of rootstock mother vines Australasian Plant Pathology 31: 425–426
Fourie PH, Halleen F (2004a) Proactive control of Petri disease of grapevine through treatment of propagation material Plant Disease 88(11): 1241–1245
Fourie PH, Halleen F (2004b) Occurrence of grapevine trunk disease pathogens in rootstock mother plants in South Africa Australasian Plant Pathology 33: 313–315
Groenewald M, Denman S, Crous PW (2000) Fungicide sensitivity of Phaeomoniella chlamydospora, the causal organism of Petri grapevine decline South African Journal of Enology and Viticulture 21: 59–61
Hawksworth DL (2001) The magnitude of fungal diversity: The 1.5 million species estimate revised Mycological Research 105: 1422–1432
Halleen F, Crous PW, Petrini O (2003) Fungi associated with healthy grapevine cuttings in nurseries, with special reference to pathogens involved in the decline of young vines Australasian Plant Pathology 32: 47–52
Jaspers MV (2001) Effect of fungicides, in vitro, on germination and growth of Phaeomoniella chlamydospora Phytopathologia Mediterranea 40(Supplement): S453–S458
Klein PG, Juneja VK (1997) Sensitive detection of viable Listeria monocytogenes by reverse transcription-PCR Applied and Environmental Microbiology 63: 4441–4448
Larignon P (1998) Esca disease from a European perspective. In: Seminar and Workshop on Black Goo Symptoms and Occurrence of Grape Declines – IAS/ICGTD Proceedings (pp. 43–55) Napa, USA
Messina J (1999) The use of beneficial Trichoderma in grapevine propagation Combined Proceedings of the International Plant Propagator’s Society 48: 145–148
Morton L (1995) Mystery diseases hit young vines Wines and Vines 11: 46–47
Mugnai L, Graniti A, Surico G (1999) Esca (black measles) and brown wood-streaking: Two old and elusive diseases of grapevines Plant Disease 83: 404–418
Olmos A, Cambra M, Esteban O, Gorris MT, Terrada E (1999) New device and method for capture, reverse transcription and nested PCR in a single closed-tube Nucleic Acids Research 27(6): 1564–1565
Retief E, Damm U, van Niekerk JM, McLeod A, Fourie PH (2005) A protocol for molecular detection of Phaeomoniella chlamydospora in grapevine wood South African Journal of Science 101: 139–142
Ridgway HJ, Sleight BE, Stewart A (2002) Molecular evidence for the presence of Phaeomoniella chlamydospora in New Zealand nurseries, and its detection in rootstock mothervines using species-specific PCR Australasian Plant Pathology 31: 267–271
Sidoti A, Buonocore E, Serges T, Mugnai L (2000) Decline of young grapevines associated with Phaeoacremonium chlamydosporum in Sicily (Italy) Phytopathologia Mediterranea 39: 87–91
Tao S, Jiang D, Lu H, Xing W, Zhou Y, Cheng J (2004) One-tube nested RT-PCR enabled by using a plastic film and its application for the rapid detection of SARS-virus Biotechnology Letters 26: 179–183
Tegli S, Bertelli E, Surico G (2000) Sequence analysis of ITS ribosomal DNA in five Phaeoacremonium species and development of a PCR-based assay for the detection of P. chlamydosporum and P. aleophilum Phytopathologia Mediterranea 39: 134–149
Torsvik V, Ovreas L (2002) Microbial diversity and function in soil: From genes to ecosystems Current Opinion in Microbiology 5: 240–245
Van der Westhuizen JH (1981) Voortplanting. In: Burger J., Deist J. (eds.) Wingerdbou in Suid-Afrika, Maskew Miller, Cape Town (pp. 141–168) (in Afrikaans)
Wallace J, Edwards J, Pascoe IG and May P (2003) Phaeomoniella chlamydospora inhibits callus formation by grapevine rootstock and scion cultivars. In: 3rd International Workshop on Grapevine Trunk Diseases. Lincoln, New Zealand (p. 24)
Whiteman SA, Jaspers MV, Stewart A, Ridgway HJ (2002) Detection of Phaeomoniella chlamydospora in soil using species-specific PCR New Zealand Plant Protection 55: 139–145
Whiteman SA, Jaspers MV, Stewart A and Ridgway HJ (2003) Identification of potential sources of Phaeomoniella chlamydospora in the grapevine propagation process. In: 8th International Congress of Plant Pathology (p. 94) Christchurch, New Zealand
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The authors would like to acknowledge the National Research Foundation (Grant number GUN2054222) and Winetech for financial support.
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Retief, E., McLeod, A. & Fourie, P.H. Potential Inoculum Sources of Phaeomoniella chlamydospora in South African Grapevine Nurseries. Eur J Plant Pathol 115, 331–339 (2006). https://doi.org/10.1007/s10658-006-9025-4
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DOI: https://doi.org/10.1007/s10658-006-9025-4