Abstract
Recent surveys of aquatic habitats suggest that organic wastewater contaminants (OWCs) may be common in aquatic ecosystems. However, relatively little is known about the impacts of OWCs on amphibians. We studied the lethal and sublethal effects of three OWCs (acetaminophen, caffeine, and triclosan) on American toad (Bufo americanus) tadpoles. High concentrations of triclosan increased activity, whereas acetaminophen had a significant effect on activity but there was no discernable pattern or trend with concentration. Caffeine did not affect activity in B. americanus tadpoles. None of the OWCs we studied had a significant effect on growth. Caffeine had no effect on survivorship. Higher concentrations of acetaminophen increased mortality. Intermediate concentrations of triclosan had a negative effect on survivorship, but the highest concentration apparently had a positive effect on survivorship. Our results suggest that there is variation in the toxicity of the three OWCs we studied.
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M. Adolfsson-Erici M. Pettersson J. Parkkoneo J. Stuarve (2002) ArticleTitleTriclosan, a commonly used bactericide found in human milk and in the aquatic environment in Sweden Chemosphere 46 1485–89
J.A. Bantle D.T. Burton D.A. Dawson J.N. Dumont R.A. Finch D.J. Fort G. Linder J.R. Rayburn D. Buchwalter A.M. Gaudet-Hull M.A. Maurice S.D. Turley (1994) ArticleTitleFETAX interlaboratory validation study: phase II testing Environ. Toxicol. Chem 13 1629–37
M.D. Boone C.M. Bridges (2003) Effects of pesticides on amphibian populations R.D. Semlitsch (Eds) Amphibian Conservation Smithsonian Institution Press Washington D.C 152–67
G.R. Boyd H. Reemtsma D.A. Grimm S. Mitra (2003) ArticleTitlePharmaceuticals and personal care products (PPCPs) in surface .and treated waters of Louisiana, USA and Ontario, Canada Sci. Total Environ 311 135–49
C.M. Bridges (2000) ArticleTitleLong-term effects of pesticide exposure at various life stages of the southern leopard frog Arch. Environ. Contam. Toxicol 39 91–96
C.M. Bridges R.D. Semlitsch (2000) ArticleTitleVariation in pesticide tolerance of tadpoles among and within species of Ranidae and patterns of amphibian decline Conserv. Biol 14 1490–99
I.J. Buerge T. Poiger. M.D. Müller H.-R. Buser (2003) ArticleTitleCaffeine, an anthropogenic marker for wastewater contamination of surface waters Environ. Sci. Technol 37 691–700
E.R. Campagnolo K. R. Johnson A. Karpati C.S. Rubin D.W. Kolpin M.T. Meyer J.E. Esteban R.W. Currier K. Smith K.M Thu M. McGeehin (2002) ArticleTitleAntimicrobial residues in animal waste and water resources proximal to large-scale swine and poultry feeding operations Sci. Total Environ 299 89–95
D.F. Cowman L.E. Mazanti (2000) Ecotoxicology of “new generation” pesticides to amphibians D.W .Sparling G. Linder C.A. Bishop (Eds) Ecotoxicology of Amphibians and Reptiles SETAC Pensacola 233–68
D.J. Fort J.R. Rayburn J.A. Bantle (1992) ArticleTitleEvaluation of acetaminophen-induced developmental toxicity using FETAX Drug Chem. Toxicol 15 329–50
D.J. Fort E.L. Stover T.L. Propst B.C. Faulkner T.A. Vollmuth F.J. Murray (1998) ArticleTitleEvaluation of the developmental toxicity of caffeine and caffeine metabolites using the frog embryo teratogenesis assay – Xenopus (FETAX) Food Chem. Toxicol 36 591–600
S.L. Fraker G.R. Smith (2004) ArticleTitleDirect and interactive effects of ecologically relevant concentrations of organic wastewater contaminants on.Rana pipiens tadpoles Environ. Toxicol 19 250–56
Fraker, S.L. and Smith, G.R. (2005). Effects of two organic wastewater contaminants on Xenopus laevis tadpoles. Appl. Herpetol. in press
P.R. Gardinali X. Zhao (2002) ArticleTitleTrace determination of caffeine in surface water samples by liquid chromatography-atmospheric pressure chemical ionization-mass spectrometry (LC-APCI-MS) Environ. Int 28 521–8
K.A. Glennemeier R.J. Denver (2001) ArticleTitleSublethal effects of chronic exposure to .an organochlorine compound on northern.leopard frog (Rana pipiens) tadpoles Environ. Toxicol 16 287–97
M.L. Harris L. Chora C.A. Bishop J.P. Bogart (2000) ArticleTitleSpecies- and age-related differences in susceptibility to pesticide-exposure for two amphibians, Rana pipiens, and Bufo americanus Bull. Environ. Contam. Toxicol 64 263–70
A.C. Hatch A.R. Blaustein (2000) ArticleTitleCombined effects of UV-B, nitrate, and low pH reduce the survival and activity level of larval cascades frogs (Rana cascadae) Arch. Environ. Contam. Toxicol 39 494–9
M. Johansson K. Räsänen J. Merilä (2001) ArticleTitleComparison of nitrate tolerances between different populations of the common frog, Rana temporaria Aquat. Toxicol 54 1–14
O.A.H. Jones N. Voulvoulis J.N. Lester (2001) ArticleTitleHuman pharmaceuticals in the aquatic environment: a review Environ. Technol 22 1383–94
OA.H. Jones N. Voulvoulis J.N. Lester (2002) ArticleTitleAquatic environmental assessment of the top 25 English prescription pharmaceuticals Water Res 36 5013–22
D.W. Kolpin E.T. Furlong M.T. Meyer E.M. Thurman S.D. Zaugg L.B. Barber H.T. Buxton (2002) ArticleTitlePharmaceuticals, hormones, and other organic wastewater contaminants in U.S. streams1999–2000: a national reconnaissance Environ. Sci. Technol 36 1202–11
G.M. Lalumera D. Calamari P. Galli S. Castiglioni G. Crosa R. Fanelli (2004) ArticleTitlePreliminary investigation on the environmental occurrence and effects of antibiotics used in aquaculture in Italy Chemosphere 54 661–8
A. Lindström I.J. Buerge T. Poiger P. Bergqvist M.D. Muller H. Buser (2002) ArticleTitleOccurrence and environmental behavior of the bactericide triclosan and its methyl derivative in surface waters and in wastewater Environ. Sci. Technol 36 2322–9
D. Morrall D. McAvoy B. Schatowitz J. Inauen M. Jacob A. Hauk W. Eckhoff (2004) ArticleTitleA field study of triclosan loss rates in river water. (Cibolo Creek, TX) Chemosphere 54 653–60
J.D. Rouse C.A. Bishop J. Stager (1999) ArticleTitleNitrogen pollution: an assessment of its threat.to amphibian survival Environ. Health Perspect 107 799–803
D. Sabaliunas S.F. Webb A. Hauk M. Jacob W.S. Eckhoff (2003) ArticleTitleEnvironmental fate of triclosan in the River Aire Basin, UK Water Res 37 3145–54
M.K. Sakamoto S. Mima T. Kihara T. Matsuo Y. Yasuda T. Tanimura. (1993) ArticleTitleDevelopmental toxicity of caffeine in the larvae of Xenopus laevis Teratology 47 189–201
R.D. Semlitsch M. Foglia A. Mueller I. Steiner E. Fioramont K. Fent (1995) ArticleTitleShort-term exposure to triphenyltin affects the swimming and feeding behavior of tadpoles Environ. Toxicol. Chem 14 1419–23
R. Siegener R.F. Chen (2002) ArticleTitleCaffeine in Boston Harbor seawater Mar. Pollut. Bull 44 383–7
H. Singer S. Muller C. Tixier L. Pillonel (2002) ArticleTitleTriclosan: occurrence and fate of a widely used biocide in the aquatic environment: field measurements in wastewater treatment plants, surface waters, and lake sediments Environ. Sci. Technol 36 4998–5004
D.W. Sparding (2000) Ecotoxicology of organic contaminants to amphibians D.W. Sparling G. Linder C.A. Bishop (Eds) Ecotoxicology of Amphibians and Reptiles SETAC Pensacola 461–94
F. Stuer-Lauridsen M. Birkved L.P. Hansen H.-C. Holten. Lützhoft B. Halling- Sørensen (2000) ArticleTitleEnvironmental risk assessment of human pharmaceuticals in Denmark after normal therapeutic use Chemosphere 40 783–93
T. Ternes M. Bonerz T. Schmidt (2001) ArticleTitleDetermination of neutral pharmaceuticals in wastewater and rivers by liquid chromatography-electrospray tandem mass spectrometry J. Chromatogr. A 938 175–85
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Smith, G.R., Burgett, A.A. Effects of Three Organic Wastewater Contaminants on American Toad, Bufo americanus, Tadpoles. Ecotoxicology 14, 477–482 (2005). https://doi.org/10.1007/s10646-004-1352-7
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DOI: https://doi.org/10.1007/s10646-004-1352-7