Abstract
Prenantȁ9s schizothoracin, Schizothorax prenanti, an endemic fish to China, has undergone a dramatic decline in numbers due to human impacts. We studied its genetic diversity in three tributaries of the Yangtze River: the Qingyi River, which has many hydropower dams, and the Dadu River and Muli River where many hydropower dams are being proposed. Using amplified fragment length polymorphism (AFLP), 621 loci were amplified with seven AFLP primer combinations in 45 individuals. The loci were highly polymorphic and heterozygous (87% polymorphism, 30% heterozygosity). The genetic distances within populations were large. The analysis of molecular variance demonstrated that most variation occurred within populations. The estimated fixation index (Φst) value averaged over all polymorphic loci across the three rivers was 0.0837, indicating a moderate genetic differentiation. The differentiations between populations were significant, and population structure was strong. The results suggested that China had wild populations of Prenantȁ9s schizothoracin with considerable genetic diversity in the Muli, Dadu and Qingyi rivers. The proposals to dam these rivers should take into account the importance of conserving their genetic quality.
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Acknowledgements
This work was funded by Science and Technology Bureau of Sichuan Province, China (No. 03JY029-071-2). We would like to thank Taiming Yan, Tianming Xie and Chunlin He for their help in sample collecting, and Fangdong Zou and Zen Zhang for their help during the experiment. We are grateful to Mart R. Gross, Aviva Patel, and two anonyms for their helpful comments on the manuscript, and Katherine Pieczora and Sean Clark for their help in English writing.
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Song, J., Song, Z., Yue, B. et al. Assessing genetic diversity of wild populations of Prenantȁ9s schizothoracin, Schizothorax prenanti, using AFLP markers. Environ Biol Fish 77, 79–86 (2006). https://doi.org/10.1007/s10641-006-9056-x
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DOI: https://doi.org/10.1007/s10641-006-9056-x