Summary
Background IMGN901 is a CD56-targeting antibody-drug conjugate designed for tumor-selective delivery of the cytotoxic maytansinoid DM1. This phase 1 study investigated the safety, tolerability, pharmacokinetics, and preliminary activity of IMGN901 in patients with CD56-expressing solid tumors. Methods Patients were enrolled in cohorts of escalating IMGN901 doses, administered intravenously, on 3 consecutive days every 21 days. A dose-expansion phase accrued patients with small cell lung cancer (SCLC), Merkel cell carcinoma (MCC), or ovarian cancer. Results Fifty-two patients were treated at doses escalating from 4 to 94 mg/m2/day. The maximum tolerated dose (MTD) was determined to be 75 mg/m2. Dose-limiting toxicities included fatigue, neuropathy, headache or meningitis-like symptoms, chest pain, dyspnea, and myalgias. In the dose-expansion phase (n = 45), seven patients received 75 mg/m2 and 38 received 60 mg/m2 for up to 21 cycles. The recommended phase 2 dose (RP2D) was established at 60 mg/m2 during dose expansion. Overall, treatment-emergent adverse events (TEAEs) were experienced by 96.9 % of all patients, the majority of which were Grade 1 or 2. The most commonly reported Grade 3 or 4 TEAEs were hyponatremia and dyspnea (each 8.2 %). Responses included 1 complete response (CR), 1 clinical CR, and 1 unconfirmed partial response (PR) in MCC; and 1 unconfirmed PR in SCLC. Stable disease was seen for 25 % of all evaluable patients who received doses ≥60 mg/m2. Conclusions The RP2D for IMGN901 of 60 mg/m2 administered for 3 consecutive days every 3 weeks was associated with an acceptable tolerability profile. Objective responses were observed in patients with advanced CD56+ cancers.
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Introduction
The glycoprotein CD56, also known as NCAM1, is a member of the neural cell adhesion molecule family [1] that plays important functional roles during development, nervous system differentiation, and immune surveillance [2]. Primarily expressed in neuroendocrine, natural killer, and T cell lineages [3], aberrant CD56 expression is seen in a variety of hematological malignancies (e.g. multiple myeloma, myelocytic and lymphocytic leukemia) as well as solid tumors (most notably small cell lung cancer [SCLC], Merkel cell carcinoma [MCC], and ovarian cancer) [4–8]. Indeed, for solid tumors, CD56 serves as a diagnostic biomarker to identify those of neuroendocrine origin, including MCC and SCLC [9, 10]. Effective treatment options for these two tumor types are limited, particularly within the context of advanced and/or refractory disease [11, 12]. CD56 has thus emerged as an attractive molecular candidate for the design of novel, targeted therapeutic strategies for improving patient outcomes in these indications.
IMGN901 (lorvotuzumab mertansine) is an antibody-drug conjugate (ADC), consisting of a humanized anti-CD56 antibody to which the tubulin-binding maytansinoid DM1 is covalently conjugated via a stable disulfide linker [13]. IMGN901 targets CD56 at the cell surface and, upon antigen binding, becomes internalized - resulting in the intracellular release of DM1 [14], which in turn promotes disruption of microtubule assembly, G2/metaphase arrest, and ultimately apoptosis [15–17]. In preclinical models, IMGN901 has shown high-affinity, antigen-specific binding and robust antitumor activity in CD56-positive (CD56+) tumors [18].
A first-in-human, open label phase I/II clinical trial has previously been conducted in patients with relapsed or refractory CD56+ SCLC and other CD56+ solid tumors where IMGN901 was administered weekly for four consecutive weeks on a six-week cycle [NCT00065429]. The results of that dose-escalation study revealed that IMGN901 displayed favorable pharmacokinetics, a manageable safety profile (including no cardiac, thyroid or adrenocortical toxicities despite CD56 expression in those tissues), and encouraging signs of activity [19, 20]. These findings prompted further evaluation of IMGN901 in the same target populations using an alternative dosing schedule. Here we report on an open label, Phase I trial of IMGN901 dosed on days 1–3 of a 21-day cycle in patients with CD56+ tumors. This regimen was selected based on prior human data showing a relatively short half-life for low doses of IMGN901. This study was undertaken to determine the tolerability, safety, and maximum tolerated dose (MTD) of IMGN901 on this schedule. Secondary assessments included pharmacokinetic profiling and preliminary assessment of clinical activity.
Patients and methods
This was a single-agent, multicenter, open-label, non-randomized, Phase I study with expansion at the MTD that involved 9 sites in the United States and the United Kingdom. The study was conducted in accordance with the US Food and Drug Administration regulations, the International Conference on Harmonisation Guidelines for Good Clinical Practice and the Declaration of Helsinki. The study was compliant with Institutional Review Board and Independent Ethics Committee requirements. All patients provided written informed consent in accordance with federal, local and institutional guidelines. This trial was registered at Clinical trials.gov (NCT00346385).
Eligibility criteria
During dose escalation, enrollment was open to patients who had histologically or cytologically proven, measurable, relapsed or refractory SCLC, neuroendocrine pulmonary tumors, metastatic MCC or carcinoid tumors, or other CD56+ solid tumors. Tumor CD56 expression was assessed prior to enrollment or at the earliest opportunity. With the exception of carcinoid and neuroendocrine tumors (which may not have been treated with chemotherapy previously), patients who had received one to three prior therapies were eligible. In the MTD expansion phase, enrollment was restricted to patients with relapsed or refractory SCLC, locally advanced or metastatic MCC, or ovarian cancer. Patients with SCLC who had received one prior chemotherapy regimen were eligible, whereas patients with MCC or ovarian cancer may have received more than one prior chemotherapy regimen. Patients with resistant or refractory ovarian cancer must have received at least one platinum-based regimen.
Eligible patients were required to be ≥18 years of age; have an Eastern Cooperative Oncology Group (ECOG) performance status ≤2; and a life expectancy of at least 3 months. Adequate hematology laboratory values and organ function were required. Patients were excluded if they had known hypersensitivity to monoclonal antibody therapy; brain metastases; or a previous malignancy with <3-year disease-free interval other than basal cell carcinoma or carcinoma in situ of the cervix.
Study design and drug administration
Enrollment of a maximum of 100 patients was planned. In the dose-escalation phase, patients received IMGN901 intravenously (IV) at 4, 8, 16, 24, 36, 48, 60, 75, or 94 mg/m2 for a maximum of 4 cycles or until intolerable toxicity, patient withdrawal or progressive disease (PD). Originally, IMGN901 was to be infused over 1 h; later, the infusion rate was reduced to 40 min. However, because of the onset of Grade 2 or higher headaches in individuals while being infused at higher doses, the protocol was amended to establish an infusion rate of 1 mg/min and prophylactic measures for all patients. These included 8 mg of oral dexamethasone (or equivalent) twice a day on the day prior to the infusion; 10 mg of IV dexamethasone 30 min prior to the infusion; and acetaminophen (500–650 mg). Patients experiencing objective responses as per RECIST 1.0 criteria [21], or those with stable disease (SD) showing clinical benefit were eligible to receive additional cycles at the same dose as that of their last treatment cycle until PD or intolerability. In the expansion phase, IMGN901 administration began at the MTD, defined as the highest dose at which <2 of six patients experienced a dose limiting toxicity (DLT), defined as grade 4 neutropenia ≥5 days, grade 4 thrombocytopenia, neutropenic infection, or any grade 3 or 4 non-hematologic toxicity (except nausea, vomiting, diarrhea, and alopecia).
Safety evaluation
All patients who enrolled and received at least one dose of study drug underwent safety evaluation (n = 97). AEs were assessed at each visit and graded according to the National Cancer Institute Common Terminology Criteria for Adverse Events Version 4.0.
Efficacy evaluation
Preliminary assessment of efficacy was performed using the RECIST response criteria version 1.0. A CT scan or MRI was performed at screening, at the end of treatment Cycles 2 and 4, at Day 22/early study treatment termination visit, at the 1-month follow-up visit, and at each short-term follow-up visit (every 6 weeks after the 1-month follow-up visit until PD or start of alternate anti-neoplastic therapy). Efficacy parameters included objective response rate (ORR), duration of response (DOR), time to progression (TTP), progression free survival (PFS) and overall survival (OS) for up to 3 years. Patients were evaluable for efficacy if they had a post-baseline radiologic or clinical assessment.
Phamacokinetics evaluation
Blood samples for pharmacokinetic (PK) characterization of intact conjugate, total huN901 antibody, human anti-human antibody, and human anti-drug antibody were collected from all patients at pre-specified time points. PK summary measures were calculated for each patient. PK analysis was performed using the standard algorithms of the non-compartmental PK analysis program (201), WinNonlin, Professional version 6.1.0.173 (Pharsight Corporation, Mountain View, California).
Statistical methods
Descriptive statistics for continuous variables were summarized using sample size (n), mean, median, standard deviation, minimum, and/or maximum. Patients who discontinued IMGN901 and started alternate anti-neoplastic therapy before documentation of PD were censored at the time of their last visit prior to initiation of alternate anti-neoplastic therapy. Patients who died or were lost to follow-up before documentation of PD or who did not progress before data cut-off were censored at the time of their last visit.
Any event with the same onset date as start of study treatment or later was reported as treatment emergent. Time-to-event variables (DOR, TTP, PFS, and OS) were based on Kaplan-Meier estimations. Baseline was defined as the last available assessment prior to Day 1, Cycle 1.
All response-evaluable patients who had a post-baseline assessment (clinical or radiologic per RECIST 1.0) were included in the Clinical Benefit Rate (CBR) analysis (complete response [CR], partial response [PR] or SD of ≥75 days in duration). A subset of this evaluable population, excluding those without post-baseline radiologic assessments, was used to evaluate the Disease Control Rate (DCR; CR, PR, or SD ≥75 days by radiologic assessment).
Results
Patient characteristics
The study accrued 97 patients, 52 in the dose-escalation phase and 45 in the expansion phase. In the dose-escalation phase, at least four patients were treated at each of the first 8 dose levels, ranging from 4 mg/m2 to 75 mg/m2, and two patients were treated at the highest dose, 94 mg/m2. In the expansion phase, the first seven patients were treated at a dose of 75 mg/m2 and the remaining 38 at 60 mg/m2.
The median age of the patients enrolled in the study was 58 years (range, 18–88) and the distribution of primary tumor types was SCLC (35.1 %), MCC (23.7 %), neuroendocrine carcinoma (15.5 %), and ovarian cancer (12.4 %) (Table 1). The majority of patients treated at 60 mg/m2 and 75 mg/m2 completed the study (86.4 % and 90.9 %, respectively), for an overall study completion rate of 86.6 %. Overall exposure ranged from 1 to 21 cycles of IMGN901 with total cumulative doses ranging from 22.5 mg to 6680 mg. Twenty-eight percent of the patients completed 3 or more cycles, with 10.3 % completing at least 6 cycles of study treatment. Thirteen patients did not complete Day 22 of cycle 1 as follows: eight patients withdrew due to disease progression and five withdrew due to an AE.
Safety and tolerability
Grade 4 fatigue in cycle 1 and Grade 2 peripheral neuropathy that progressed to Grade 3 in cycle 2 were reported as DLTs in the 16 mg/m2 (n = 1) and 48 mg/m2 (n = 1) cohorts. At the 75 mg/m2/day dose level, an initial cohort of four patients was enrolled. One patient experienced a DLT of Grade 4 headache associated with meningitis-like symptoms, prompting a reduction in the infusion rate. An additional four patients were treated with the slowed infusion rate, two of whom developed Grade 2 headaches that in turn prompted the administration of prophylactic steroids. Of a total of seven additional patients in the steroid pre-treated 75 mg/m2 cohort, one developed neuropathy in cycle 1 that worsened to a DLT (Grade 3 paresthesia) in cycle 3. Dose escalation continued to 94 mg/m2 with two patients who experienced pain-related DLTs on day 8 of cycle 1 (Grade 3 headache, pain, and chest pain in one patient and Grade 3 myalgia in the other). Therefore, the 75-mg/m2 dose was declared the MTD. However, because three of the first seven patients treated at the 75-mg/m2 dose experienced drug-related serious adverse events (SAEs) (generalized pain and increased chest pain [n = 1]; fatigue, syncope, Grade 2 neuropathy, and myalgia [n = 1]; and Grade 3 dyspnea, Grade 2 myalgia, chest pain, and fatigue [n = 1]), a dose of 60 mg/m2/day was explored, and ultimately confirmed as the recommended phase II dose (RP2D).
Ninety-four patients (96.9 %) reported a treatment-emergent adverse event (TEAE), the majority of which were Grade 1 or 2; with nausea (43.3 %), fatigue (39.2 %), and constipation (37.1 %) being the most commonly reported. Table 2 summarizes those TEAEs reported for ≥5 % of patients that were assessed as possibly, probably, or definitely related (“related”) to study treatment (75.3 %). The most common treatment-related TEAEs were nausea (30.9 %), fatigue (27.8 %), headache (26.8 %), peripheral neuropathy (17.5 %), and vomiting (16.5 %).
Seventy-eight patients (80.4 %) reported a Grade 3 or 4 TEAE; 37.1 % were assessed as treatment-related. Overall, gastrointestinal (25.8 %), metabolism and nutrition (19.6 %), investigation (18.6 %), and nervous system (15.5 %) disorders accounted for the highest incidence of Grade 3 or 4 AEs. The most commonly reported were hyponatremia (8.2 %), dyspnea (8.2 %), and elevated gamma glutamyltransferase (GGT; 7.2 %). Clinically significant elevations of pancreatic enzymes (serum amylase, lipase, and/or trypsinogen) were seen in 16 % of the patients treated at 60 mg/m2 and in one of four (25 %) patients in the 24-mg/m2 cohort; however, only two patients experienced an AE of pancreatitis.
SAEs were reported for 53 patients (54.6 %) and treatment-related SAEs were experienced by 15 patients (15.5 %). These included constipation (3.1 %); headache (3.1 %); pain, fatigue, pain in extremity, and chest pain (2.1 %); and abdominal pain and fever (1.0 %). One patient treated at 60 mg/m2 experienced reversible posterior leukoencephalopathy syndrome with cortical blindness [22]. Twelve patients experienced a SAE with an outcome of death. However, only one of these SAEs (bronchopneumonia), which occurred in a patient treated at 75-mg/m2, was judged to be related to study treatment.
Efficacy
Clinical benefit was observed in 25.5 % of all patients, with a CBR of 27.3 % in the cohort of individuals treated at the 60 mg/m2 dose level (Table 3). These values included SD lasting ≥75 days being seen in 25 % of patients who received a dose of 60 mg/m2 or higher (n = 68). Four patients met RECIST criteria for objective responses (Fig. 1 and Table 4) as follows: one patient with MCC who had received IMGN901 at 36 mg/m2 had a confirmed CR; a second MCC patient treated at 60 mg/m2 had a confirmed PR that progressed to clinical CR but refused further CT assessment and, therefore, could be evaluated only by clinical assessment; and two patients, one with MCC (60 mg/m2) and another with SCLC (75 mg/m2) had unconfirmed PRs. Overall disease control rates (DCR values) were 18.2 % for all evaluable patients and 22.5 % for those treated at 60 mg/m2.
These findings revealed that the most notable activity was observed in patients with metastatic MCC (n = 23), with thirteen percent overall demonstrating responses to treatment (Table 5). One patient received a total of 6 cycles of treatment with IMGN901 at 36 mg/m2 and achieved a PR after cycle 1 and a CR by cycle 3. The patient remained in complete remission for nearly 6 years until dying from non-small cell lung cancer (NSCLC). Another patient had a PR after cycle 1 of treatment with IMGN901 at 60 mg/m2 (65 % reduction in target skin lesions), but developed reversible posterior leukoencephalopathy syndrome and was discontinued. This patient achieved a clinical CR with resolution of all skin lesions 2 years after treatment with a single course of IMGN901 and was still alive with no signs of disease progression at the last follow-up visit (~2.8 years). A third patient, who presented with widespread metastatic disease at baseline, developed PD after cycle 2 of treatment. Interestingly, whole body PET CT scan performed 8 months after study discontinuation revealed no evidence of FDG-avid disease. Subsequently, he experienced waxing and waning low-volume disease (<1 cm diameter of some lesions), but the patient was alive with no signs of PD 2.4 years after receiving the first dose of IMGN901 and without having received any additional therapy. Two patients achieved clinically relevant SD; one of them remained on study for 10 cycles, whereas the other received 21 cycles of treatment and was discontinued due to an AE of ataxia.
Clinical activity was also observed in additional tumor types. The CBR for patients with ovarian cancer (n = 12) was 33.3 %. One patient in this group received 12 cycles of therapy with IMGN901 before developing PD and was alive at 1.9 years after her first dose of IMGN901. Patients with SCLC demonstrated a CBR of 21.2 % (n = 33), with one patient achieving an unconfirmed PR and six patients experiencing SD.
The median PFS and TTP were identical for both the overall population (2.1 months; CI = 1.2, 1.5 months) and for the subset of patients treated with 60 mg/m2 of IMGN901 (1.3 months; CI = 1.2, 1.6 months) (Table 2). Median OS for all patients was 9.2 months (CI = 5.7, 13.2 months), whereas median OS for patients treated at 60 mg/m2 was 8.1 months (CI = 5.5, 14.3 months).
Pharmacokinetics
The maximum plasma concentration (Cmax) and area under the concentration curve (AUC0-∞) of IMGN901 generally increased with increasing dose (Fig. 2a, 2b). These parameters also increased with subsequent treatments within a cycle (Cmax shown in Fig. 2c). At the 60 mg/m2 dose, a consistent elevation in the median Cmax (about 1.5-fold on day 3) was observed. The half-life of IMGN901 at the 60 mg/m2 dose was approximately 30 to 40 h following Day 3 of cycle 1. The concentration-time profiles for total huN901 antibody were similar to those of IMGN901 (data not shown). Total huN901 antibody had a slightly longer elimination half-life than IMGN901 at doses ≥60 mg/m2.
Discussion
In this study, we evaluated the safety, tolerability, pharmacokinetics and preliminary efficacy of single agent IMGN901 administered on Days 1–3 of a 21-day cycle to patients with CD56-positive solid tumors. Ninety-seven patients were accrued. Patients in the dose-escalation phase received IMGN901 at doses ranging from 4 to 94 mg/m2. The MTD was declared at 75 mg/m2 and the first seven patients enrolled into the dose-expansion phase were treated at that dose level. During drug administration, however, three patients experienced dose-limiting headache with or without meningitis-like symptoms. These events prompted the implementation of a slower infusion rate and prophylactic low-dose steroids and acetominophen, which reduced the incidence and severity of these symptoms. Similar toxicity was observed in the first-in-human study, with symptoms developing within 6 to 8 h post-infusion and resolving within 1 to 3 days [19, 23, 24]. Despite these measures, 3 of 7 patients experienced drug-related SAEs at 75 mg/m2, thus the RP2D was determined to be 60 mg/m2.
Preliminary evidence of antitumor activity of IMGN901 was noted in several CD56+ tumor types. Of the 94 patients in the efficacy evaluable population, 25.5 % experienced clinical benefit. Patients treated with 60 mg/m2 experienced the highest CBR (27.3 %). Four patients, three of whom were diagnosed with MCC, had objective responses. While the ORR was modest, it should be noted that all patients had previously been treated with other therapies and almost half (49 %) had received two or more prior lines of treatment.
The most interesting signals of clinical activity were seen in patients with metastatic MCC, a rare and aggressive cutaneous malignancy of neuroendocrine origin. Surgical excision of the primary tumor is the first line of treatment; however, MCC has a high propensity for local recurrence as well as regional and distant metastases [25, 26]. Current therapies have demonstrated no survival benefit for patients with distal metastatic disease [27], and for this patient population, treatment is limited to palliative care [28]. As noted above, three objective and durable responses and an overall CBR of 21.7 % were observed among the 23 patients with metastatic MCC recruited to the study. Together with preliminary signs of efficacy in SCLC and ovarian cancer patients, these results provide encouraging evidence of single-agent IMGN901 activity.
Analysis of CD56 expression demonstrated strong expression in the majority of the tumors. Despite this, not all patients derived meaningful clinical benefit. A potential explanation for this may involve acquired resistance to the microtubule-targeting payload among the heavily pre-treated patients enrolled in this study. For example, SCLC is usually responsive to initial platinum-based chemotherapy, but subsequent chemotherapy rarely results in response and shows only modest improvements in survival [29]. Furthermore, preclinical evidence suggests that induced resistance to cisplatin can render SCLC cells cross-resistant to the microtubule-disrupting vinca alkaloids [30]. Other factors that may contribute to lack of response in some patients include heterogeneous CD56 expression within a tumor, or varying rates of ADC internalization. The half-life of IMGN901 is relatively short compared with that of other humanized IgG1 antibodies; this can be explained, at least in part, by high antigen-mediated clearance due to CD56 expression on a variety of normal tissues, in particular highly abundant NK cells. This may serve to restrict IMGN901 exposure, which, in turn, may limit activity to tumors exquisitely sensitive to the maytansinoid payload.
In conclusion, IMGN901 administered IV at a dose of 60 mg/m2 on three consecutive days every 3 weeks exhibited acceptable safety and tolerability. Objective, durable responses in a number of patients with MCC, and durable SD observed in several previously treated patients with relapsed advanced-stage CD56+ cancers, are encouraging signs of single-agent IMGN901 activity. The clinical evaluation of IMGN901 is ongoing, with two Phase II studies, one in CD56-expressing hematological malignancies [NCT02420873] and the other in pediatric neuroendocrine-derived tumors [NCT02452554], having recently been initiated.
References
Zeromski J, Nyczak E, Dyszkiewicz W (2001) Significance of cell adhesion molecules, CD56/NCAM in particular, in human tumor growth and spreading. Folia Histochem Cytobiol 39(Suppl 2):36–37
NCAM1 neural cell adhesion molecule 1 (2015) www.ncbi.nlm.nih.gov/gene/4684. Accessed 23 Dec 2015
Cunningham BA, Hemperly JJ, Murray BA, Prediger EA, Brackenbury R, et al. (1987) Neural cell adhesion molecule: structure, immunoglobulin-like domains, cell surface modulation, and alternative RNA splicing. Science 236:799–806
Griffin JD, Hercend T, Beveridge R, Schlossman SF (1983) Characterization of an antigen expressed by human natural killer cells. J Immunol 130:2947–2951
Patel K, Moore SE, Dickson G, Rossell RJ, Beverley PC, et al. (1989) Neural cell adhesion molecule (NCAM) is the antigen recognized by monoclonal antibodies of similar specificity in small-cell lung carcinoma and neuroblastoma. Int J Cancer 44:573–578
Kurokawa M, Nabeshima K, Akiyama Y, Maeda S, Nishida T, et al. (2003) CD56: a useful marker for diagnosing Merkel cell carcinoma. J Dermatol Sci 31:219–224
Ohishi Y, Kaku T, Oya M, Kobayashi H, Wake N, et al. (2007) CD56 expression in ovarian granulosa cell tumors, and its diagnostic utility and pitfalls. Gynecol Oncol 107:30–38
Guo W, Xing C, Dong A, Lin X, Lin Y, et al. (2013) Numbers and cytotoxicities of CD3 + CD56+ T lymphocytes in peripheral blood of patients with acute myeloid leukemia and acute lymphocytic leukemia. Cancer Biol Ther 14:916–921
McNiff JM, Cowper SE, Lazova R, Subtil A, Glusac EJ (2005) CD56 staining in Merkel cell carcinoma and natural killer-cell lymphoma: magic bullet, diagnostic pitfall, or both? J Cutan Pathol 32:541–545
Lantuejoul S, Moro D, Michalides RJ, Brambilla C, Brambilla E (1998) Neural cell adhesion molecules (NCAM) and NCAM-PSA expression in neuroendocrine lung tumors. Am J Surg Pathol 22:1267–1276
Erovic I, Erovic BM (2013) Merkel cell carcinoma: the past, the present, and the future. J Skin Cancer 2013:929364
Asai N, Ohkuni Y, Kaneko N, Yamaguchi E, Kubo A (2014) Relapsed small cell lung cancer: treatment options and latest developments. Ther Adv Med Oncol 6:69–82
Lambert JM, O’Leary J, Whiteman KR, Goldmacher VS (2012) Targeting CD56 (NCAM)-expressing neoplasms with lorvotuzumab mertansine. In: Phillips GL (ed) Antibody-drug conjugates and immunoglobulins: from pre-clinical development to therapeutic applications. Springer, New York, pp. 273–296
Tijink BM, Buter J, de Bree R, Giaccone G, Lang MS, et al. (2006) A phase I dose escalation study with anti-CD44v6 bivatuzumab mertansine in patients with incurable squamous cell carcinoma of the head and neck or esophagus. Clin Cancer Res 12:6064–6072
Chari RV, Martell BA, Gross JL, Cook SB, Shah SA, et al. (1992) Immunoconjugates containing novel maytansinoids: promising anticancer drugs. Cancer Res 52:127–131
Erickson HK, Lambert JM (2012) ADME of antibody-maytansinoid conjugates. AAPS J 14:799–805
Ballantyne A, Dhillon S (2013) Trastuzumab emtansine: first global approval. Drugs 73:755–765
Whiteman KR, Johnson HA, Mayo MF, Audette CA, Carrigan CN, et al. (2014) Lorvotuzumab mertansine, a CD56-targeting antibody-drug conjugate with potent antitumor activity against small cell lung cancer in human xenograft models. MAbs 6:556–566
Fossella F, McCann J, Tolcher A, Xie H, Hwang LL et al (2005) Phase II trial of BB-10901 (huN901-DM1) given weekly for four consecutive weeks every 6 weeks in patients with relapsed SCLC and CD56-positive small cell carcinoma. J Clin Oncol 23 (suppl; abstr 7159)
Fossella F, Woll P, Lorigan P, Tolcher A, O’Brien M, et al. (2009) Clinical experience of IMGN901 (BB-10901) in patients with small cell lung carcinoma (SCLC). J Thorac Oncol 4(Suppl):S465
Therasse P, Arbuck SG, Eisenhauer EA, Wanders J, Kaplan RS, et al. (2000) New guidelines to evaluate the response to treatment in solid tumors. European Organization for research and treatment of cancer, National Cancer Institute of the United States, National Cancer Institute of Canada. J Natl Cancer Inst 92:205–216
Wilson C, Leaning D, Shankland K, Langheinrich T, Battya R, et al. (2010) Cortical blindness as an unusual adverse drug reaction. J Med Cases 1:47–50
Woll PJ, O’Brien M, Fossella F, Shah SA, Clinch Y et al (2010) Phase I study of lorvotuzumab mertansine (IMGN901) in patients with CD56-positive solid tumors. Ann Oncol 21 (suppl; abstr 536P)
Tolcher A, Forouzesh B, McCreery H, Hammond L, Patnaik A, et al. (2002) A phase I and pharmacokinetic study of BB-10901, a maytansinoid immunoconjugate, in CD56 expressing tumors. Eur J Cancer 38(Suppl 7):S152–S153
Soult MC, Feliberti EC, Silverberg ML, Perry RR (2012) Merkel cell carcinoma: high recurrence rate despite aggressive treatment. J Surg Res 177:75–80
Mojica P, Smith D, Ellenhorn JD (2007) Adjuvant radiation therapy is associated with improved survival in Merkel cell carcinoma of the skin. J Clin Oncol 25:1043–1047
Hughes MP, Hardee ME, Cornelius LA, Hutchins LF, Becker JC, et al. (2014) Merkel cell carcinoma: epidemiology, Target, and therapy. Curr Dermatol Rep 3:46–53
Schlaak M, Podewski T, Von Bartenwerffer W, Kreuzberg N, Bangard C, et al. (2012) Induction of durable responses by oral etoposide monochemotherapy in patients with metastatic Merkel cell carcinoma. Eur J Dermatol 22:187–191
Schiller JH, Adak S, Cella D, DeVore RF 3rd, Johnson DH (2001) Topotecan versus observation after cisplatin plus etoposide in extensive-stage small-cell lung cancer: E7593–a phase III trial of the Eastern Cooperative Oncology Group. J Clin Oncol 19:2114–2122
Jain N, Lam YM, Pym J, Campling BG (1996) Mechanisms of resistance of human small cell lung cancer lines selected in VP-16 and cisplatin. Cancer 77:1797–1808
Acknowledgments
Thanks to all the patients who participated. We thank Sarah Scafidi, Mary McKenzie, Robert Fram, and James O’Leary (ImmunoGen). We also thank Monica Moore and William Fiedelman for contributing to the editing and writing of the manuscript. Penella J. Woll (Sheffield Experimental Cancer Medicine Centre) acknowledges support from Cancer Research UK and the UK Department of Health and Mary E.R. O’Brien (Royal Marsden Hospital) from the NIHR/ICR Biomedical Research Centre.
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MK is an employee of ImmunoGen, Inc. FVF acknowledges support from ImmunoGen, Inc. KNM has been a member of the Speakers Bureau for Genentech/Roche and a consultant/advisory board member of Genentech, ImmunoGen Inc., Advaxis, and Astra Zeneca. The remaining authors declare no conflict of interest.
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All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.
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Written informed consent was obtained from all participants included in the study.
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Shah, M.H., Lorigan, P., O’Brien, M.E.R. et al. Phase I study of IMGN901, a CD56-targeting antibody-drug conjugate, in patients with CD56-positive solid tumors. Invest New Drugs 34, 290–299 (2016). https://doi.org/10.1007/s10637-016-0336-9
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DOI: https://doi.org/10.1007/s10637-016-0336-9