Abstract
Background
Colorectal cancer (CRC) is a common cancer with high metastatic property. Circular RNAs (circRNAs) have important involvement in cancer processes. This study focused on the regulation of circRNA RAD23 homologue B (circRAD23B) in CRC.
Methods
The levels of circRAD23B, microRNA-1205 (miR-1205), and tripartite motif-44 (TRIM44) were examined by quantitative real-time polymerase chain reaction (qRT-PCR). Functional analyses were performed by Cell Counting Kit-8 (CCK-8) for cell proliferation, flow cytometry for cell cycle or cell apoptosis, and transwell assay for cell migration and invasion. Western blot was administrated for protein detection. The interaction of targets was analyzed by dual-luciferase reporter and RNA pull-down assays. The in vivo experiment was conducted via xenograft tumor in mice.
Results
We identified that circRAD23B was overexpressed in CRC tissues and cells. CRC cell proliferation, cell cycle progression, and cell metastasis were inhibited, while apoptosis was promoted by downregulating circRAD23B. Target analysis indicated that circRAD23B-targeted miR-1205 and TRIM44 were downstream genes of miR-1205. Moreover, the antitumor response of circRAD23B downregulation and miR-1205 overexpression was, respectively, achieved by increasing miR-1205 and decreasing TRIM44. CircRAD23B could regulate TRIM44 level by sponging miR-1205. In vivo, circRAD23B knockdown also reduced CRC tumorigenesis via the miR-1205/TRIM44 axis.
Conclusion
These results suggested that the inhibition of circRAD23B retarded the progression of CRC via acting on the miR-1205/TRIM44 axis. CircRAD23B might be a novel target in CRC treatment.
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Introduction
Colorectal cancer (CRC) is an aggressive disease with enormous challenges in diagnostic and therapeutic optimization to reduce mortality in the clinic [1]. Molecular diagnostics with high accuracy and sensitivity have been developed as effective tools in screening and diagnosing CRC [2]. In addition, some cellular biomolecules were reported to monitor the therapeutic process for CRC [3]. To seek novel targets used for the diagnosis and treatment of CRC is quite necessary.
Circular RNAs (circRNAs) are a novel family of endogenous noncoding RNAs (ncRNAs) with ubiquitous expression in eukaryotes and can affect various cancer cellular phenotypes [4]. CircRNAs play as functional microRNA (miRNA) sponges to regulate the expression of downstream genes [5]. Lu et al. reported that silencing of circ-FARSA suppressed CRC cell growth and metastasis by inhibiting the miR-330-5p-mediated LASP1 expression [6]. Circ_0007142 promoted CDC25A to induce CRC malignance via sponging miR-122-5p [7], and hsa_circ_0008285 was a tumor-inhibitory factor in CRC via the regulation of miR-382-5p/PTEN axis [8].
CircRNA RAD23 homologue B (circRAD23B) has been identified as an oncogene in lung cancer [9] and esophageal cancer [10]. Han et al. found that circRAD23B enhanced cell growth and invasion in nonsmall cell lung cancer via the miR-593-3p/CCND2 or miR-653-5p/TIAM1 signal axis [11]. However, circRAD23B is an unexplored circRNA in CRC. Previous studies have suggested that microRNA-1205 (miR-1205) played an inhibitory role in epithelial-to-mesenchymal transition (EMT) of CRC cells [12] and tripartite motif-44 (TRIM44) served for a cancer-promoting gene in CRC [13, 14].
In this study, the functional investigation of circRAD23B in CRC will be performed. Moreover, we put forward the hypothesis that circRAD23B acted as a miR-1205 sponge to regulate TRIM44 in CCRC and affirmed it by performing a series of experiments.
Materials and Methods
Acquisition of Human Samples
The current study was approved by the Ethics Review Committee of Weifang No.2 People’s Hospital, and all protocols strictly followed the Declaration of Helsinki involving human subjects. After surgical resection of CRC patients at Weifang No.2 People’s Hospital, we have collected 50 paired CRC and the corresponding normal tissues (away from cancer tissues for more than 2 cm). These human samples were snap-frozen in liquid nitrogen and then preserved at − 80 °C. Fifty patients have provided written informed consent before surgery. The inclusion criteria of patients were shown as below: (1) patients were diagnosed as CRC after pathological examination; (2) patients have not received radiotherapy or chemotherapy prior to the surgery; and (3) patients were voluntarily involved in this study. The clinicopathologic features of patients are presented in Table 1.
Cell Culture
Human normal enterocyte NCM460 (QCB309; QCHENG BIO, Shanghai, China) was maintained in Dulbecco’s modified eagle medium (DMEM; Hyclone, Logan, UT, USA). CRC cell lines SW480 (CCL-228) and SW620 (CCL-227) were purchased from American Type Culture Collection (ATCC, Manassas, VA, USA) and sustained in L-15 medium (Hyclone). 10% fetal bovine serum (FBS; Sigma-Aldrich, St. Louis, MO, USA) and 1% penicillin–streptomycin solution (Millipore, Billerica, MA, USA) were complemented for great cell growth. Cells were cultured under the conditions of 5% CO2 at 37 °C.
Oligonucleotides or Vectors Transfection
After cell seeding overnight to 70% confluence, oligonucleotides or vectors were transfected into SW480 and SW620 cells by Lipofectamine™ 3000 Transfection Reagent (Invitrogen, Carlsbad, CA, USA). The oligonucleotides were directly bought from RIBOBIO (Guangzhou, China): small interfering RNAs (siRNAs) targeting circRAD23B (si-circRAD23B#1, si-circRAD23B#2, si-circRAD23B#3), miR-1205 mimic and inhibitor (miR-1205 and anti-miR-1205), and the matched negative controls (si-NC, miR-NC, and anti-miR-NC). For the overexpression of circRAD23B and TRIM44, their sequences were, respectively, cloned into pcircRNA 2.2 hsa vector (pcirc; BersinBio, Guangzhou, China) and pcDNA vector (Invitrogen) to obtain the recombinant vectors circRAD23B and TRIM44.
RNA Isolation, Treatment, and the Quantitative Real-Time Polymerase Chain Reaction (qRT-PCR)
TRI reagent (Sigma-Aldrich) was used for processing tissues and cells to acquire total RNA. For RNase R treatment, 2 µg RNA was added with 6 U RNase R (Epicentre Technologies, Madison, WI, USA) at 37 °C for 1 h. The complementary DNA (cDNA) was synthesized using cDNA Synthesis Supermix (Bio-Rad, Hercules, CA, USA), and then the qRT-PCR was performed by iTaq Universal SYBR Green Supermix (Bio-Rad) on the CFX96 Dx Real-Time PCR System (Bio-Rad). Glyceraldehyde phosphate dehydrogenase (GAPDH) and U6 served as the housekeeping genes in this study, respectively, for circRNA/mRNA and miRNA. The 2−∆∆Ct method was used to analyze the relative levels of genes. Primers sequences of forward (F) and reverse (R) were as follows: circRAD23B, 5′-GCAAGTAATTGCAGCCCTGAG-3′ (F) and 5′-GCTGAGTTGTAGCTGGTGCT-3′ (R); RAD23B, 5′-CTTCCTCCACCACCACAACT-3′ (F) and 5′-GGTGTCTCTGCTGGCTTTTC-3′ (R); miR-1205, 5′-CTGCAGGGTTTGCTTTGAGG-3′ (F) and 5′-CTCCAGAACAGGGTTGACAGG-3′ (R); TRIM44, 5′-GTGGACATCCAAGAGGCAAT-3′ (F) and 5′-AGCAAGCCTTCATGTGTCCT-3′ (R); GAPDH, 5′-CTCTGCTCCTCCTGTTCGAC-3′ (F) and 5′-GCGCCCAATACGACCAAATC-3′ (R); and U6, 5′-GCTTCGGCAGCACATATACTAAAAT-3′ (F) and 5′-CGCTTCACGAATTTGCGTGTCAT-3′ (R).
Cell Proliferation Assay
1 × 104 SW480 and SW620 cells were suspended in 100 µL L-15 medium and then transplanted into the 96-well plates. After 24-h culture, cell transfection was performed for 0 h, 24 h, 48 h, and 72 h, respectively. 10 µL/well CCK-8 solution (Nanjing Jiancheng Bioengineering Institute, Nanjing, China) was added into the wells with no bubble. The absorbance at the wavelength of 450 nm was detected via a microplate reader (Bio-Rad) 3 h later.
Cell Apoptosis Detection
Apoptotic generation in cells was determined using Annexin V-FITC/PI double-staining apoptosis detection kit (Nanjing Jiancheng Bioengineering Institute) by flow cytometry. In brief, cells were harvested through the digestion of trypsin (Sigma-Aldrich) after 72-h transfection. Then, cells were resuspended in phosphate buffer solution (PBS; Hyclone) and 3 × 105 cell pellets were mixed with 500 µL 1 × binding buffer. After the addition of 5 µL Annexin V-FITC and 5 µL propidium iodide (PI) for 10 min in the dark, cell detection was instantly conducted under the flow cytometer (BD Biosciences, San Diego, CA, USA) with Annexin V-FITC as green fluorescence and PI as red fluorescence. The apoptotic cells indicated those cells stained by Annexin V-FITC (+)/PI(−) and Annexin V-FITC(+)/PI(+).
Cell Cycle Analysis
Cell cycle proportion was examined via Cell cycle detection kit (Nanjing Jiancheng Bioengineering Institute) by flow cytometry. 5 × 105 collected cells were suspended in 300 µL PBS (Hyclone), and then cell suspension was added to 1.2 mL ice-cold ethanol (Sigma-Aldrich) at − 20 °C overnight. Thereafter, cells were centrifuged at 2000 rpm for 5 min and washed with 1 mL PBS, followed by the resuspension in 100 µL RNase A solution at 37 °C for 30 min. Four hundred microliters of PI was then mixed with cells at 4 °C for 30 min away from light, and the absorbance at 488 nm was read by a flow cytometer (BD Biosciences).
Cell Migration and Invasion Examination
Cell migration and invasion were examined in transwell chamber (Corning Inc., Corning, NY, USA) and transwell chamber precoated with matrigel (Corning Inc.). The upper chamber was added with transfected cells in serum-free medium, and the complete medium was added into the lower chamber. After cell seeding for 24 h, cells on the lower surface of the polycarbonate membrane were fixated in 4% paraformaldehyde (Sigma-Aldrich) and stained by crystal violet (Sigma-Aldrich). Cell image acquisition (100 × magnification) and cell counting were performed under an inverted microscope (Olympus, Tokyo, Japan).
Western Blot
Total proteins were rapidly and efficiently isolated from CRC tissues and cells applying with RIPA Buffer (Sigma-Aldrich). The protein levels of different genes were detected referring to the previous study [15] using the following antibody: anti-matrix metalloproteinase 2 (anti-MMP2; Abcam, Cambridge, UK, ab97779, 1:1000), anti-MMP9 (Abcam, ab38898, 1:1000) and internal control GAPDH (Abcam, ab9485, 1:3000). After the incubation of Goat Anti-Rabbit IgG H&L (HRP) (Abcam, ab205718, 1:5000), the protein bands were presented via the coloration by ECL Western Blotting Substrate Kit (Abcam). Ultimately, the protein quantification was performed using Labworks™ Analysis Software version 4.0 (Labworks, Upland, CA, USA).
Dual-Luciferase Reporter Assay
The luciferase reporter plasmids of wild-type (WT) and mutant-type (MUT) circRAD23B (circRAD23B WT and circRAD23B MUT) or TRIM44 (TRIM44 3′UTR WT and TRIM44 3′UTR MUT) were constructed using the basic luciferase vector pGL-3 control (Promega, Madison, WI, USA). At 48 h after cotransfection of circRAD23B WT/circRAD23B MUT/TRIM44 3′UTR WT/TRIM44 3′UTR MUT and miR-1205/miR-NC, SW480 and SW620 cells were collected and the relative luciferase intensity was measured by the Dual-Luciferase Reporter Assay System (Promega) as per the guidance for users.
Pull-Down Assay with Biotinylated miR-1205
To analyze the binding between miR-1205 and TRIM44, Biotin-coupled miR-1205 (Bio-miR-1205) and negative control Bio-miR-NC were, respectively, transfected into SW480 and SW620 cells. Cell lysates were then incubated in Streptavidin Magnetic Beads (Thermo Fisher Scientific, Waltham, MA, USA), and the RNA complexes were extracted. Employing the cDNA as the amplified templates, TRIM44 mRNA expression was assayed using qRT-PCR as mentioned above.
Xenograft Tumor Assay
Six-week-old male BALB/c nude mice were purchased from Shanghai SIPPR-BK Laboratory Animal Co. Ltd. (Shanghai, China). Mice were cared in the specific pathogen-free (SPF) environment with a constant temperature of 26 °C, a humidity of 70%, and a programmed cycle (12-h light/12-h dark) for circadian control. 5 × 106 transfected SW480 cells were suspended in 200 µL PBS, and the prepared cell suspension was subcutaneously injected into the flanks of mice, with five mice in sh-NC or sh-circRAD23B group. Every 7 days after cell injection, tumor size was evaluated by the length (L) and weight (W), followed by the calculation of tumor volume using the standard formula: L × W2 × 0.5. Tumors were isolated from killed mice after 28 days, and tumors were weighed. The genic detection was carried out by qRT-PCR and western blot after purification of RNA and protein. All mice were painlessly killed by CO2 asphyxia method (using CO2 to replace the air of container with 30% of volume/min), and the killed mice were buried after tissue collection. This animal assay was empowered by Animal Ethics Committee of Weifang No.2 People’s Hospital, and the experiments on mice were implemented strictly according to the principles for the Care and Use of Laboratory Animals of National Institute of Health.
Statistical Analysis
In this study, data expressed as the mean ± standard deviation (SD) were statistically analyzed via SPSS 20.0 (SPSS Inc., Chicago, IL, USA). Student’s t test was applied for the comparison between two groups, and difference analyses among multiple groups were administrated via one-way analysis of variance (ANOVA) followed by Tukey’s test. The difference was significant when P < 0.05.
Results
CircRAD23B Was Highly Expressed in CRC Tissues and Cells
Gene Expression Omnibus (GEO) dataset GSE126094 (https://www.ncbi.nlm.nih.gov/geo/geo2r/?acc=GSE126094) indicated that circRAD23B was upregulated in human CRC samples compared with Normal samples (Fig. 1a). According to the cutoff criteria of values of |log2 fold change| (|log2FC|) > 1 and P < 0.05, there were many dysregulated circRNAs (1831 upregulated and 20 downregulated) in the GSE126094 dataset (Fig. 1b). CircRAD23B is one of the upregulated circRNAs derived from the Exon4-6 of RAD23B gene with the closed-loop structure (Fig. 1c). The upregulation of circRAD23B was also found in our collected CRC tissues and cultured cells (SW480 and SW620) relative to Normal tissues and NCM460 cell line (Fig. 1d, e). Clinicopathologic features indicated that high circRAD23B expression was related to tumor TNM stage (P = 0.004) and tumor metastasis (P = 0.023) in CRC patients (Table 1). Besides, circRAD23B was more resistant to exonuclease RNase R than linear RAD23B in SW480 and SW620 cells (Fig. 1f, g), showing the stability of circRAD23B as a circRNA. Thus, circRAD23B was abnormally upregulated in CRC.
Downregulating circRAD23B Inhibited the Cancer Progression in CRC Cells
The interference of circRAD23B was achieved by siRNA transfection as illustrated in Fig. 2a, b, circRAD23B level was much lower in siRNA groups (si-circRAD23B#1, si-circRAD23B#2, si-circRAD23B#3) than that in si-NC group. We then performed the functional analysis of circRAD23B via exploring the effects of si-circRAD23B#2 (with more significant knockdown efficiency) on various cellular behaviors. An inhibitory phenomenon of cell proliferation in CCK-8 assay (Fig. 2c, d) and a promoting effect on cell apoptosis in flow cytometry (Fig. 2e, f) were caused in SW480 and SW620 cells, as the consequences of circRAD23B downregulation. Additionally, knockdown of circRAD23B was shown to elevate cell distribution of G0/G1 phase and decrease that of S phase (with no difference of G2/M phase), resulting in the retardation of cell cycle (Fig. 2g, h). Transwell assay demonstrated that the migratory ability (Fig. 2i) and invasive potential (Fig. 2j) were reduced in si-circRAD23B#2-trasnfetced SW480 and SW620 cells compared to si-NC-transfected cells. Also, the invasion-related MMP2 and MMP9 protein levels were found to be repressed with the knockdown of circRAD23B (Fig. 2k, l). Altogether, CRC progression was inhibited by the downregulation of circRAD23B in vitro.
CircRAD23B Sponged miR-1205 in CRC Cells
After the target prediction by circinteractome and circBank and Venn diagram analysis, four miRNAs (miR-1205, miR-1253, miR-892b, miR-935) were screened as the candidate targets of circRAD23B. The qRT-PCR indicated that only miR-1205 expression was affected by si-circRAD23B#2 with 2.5-fold increase and we then chose miR-1205 as a target subject for circRAD23B (Supplementary Fig. 1). To analyze the effects of circRAD23B on miR-1205 level in CRC, SW480 and SW620 cells were transfected with si-NC or si-circRAD23B#2 and pcirc or circRAD23B. The qRT-PCR result displayed that silencing circRAD23B upregulated the expression of miR-1205 (Fig. 3a, b), and it was opposite that miR-1205 was downregulated by circRAD23B overexpression (Fig. 3c, d). The online circBank exhibited the binding sites for miR-1205 in the sequence of circRAD23B (Fig. 3e). Dual-luciferase reporter assay further manifested that miR-1205 mimic led to the luciferase inhibition in circRAD23B WT plasmid and no change was observed in circRAD23B MUT group (Fig. 3f, g), which affirmed the binding between circRAD23B and miR-1205. The miR-1205 expression was much lower in CRC tissues (Fig. 3h) and cells (Fig. 3i) by comparison with control tissues and cells. All these data showed that circRAD23B could generate the sponge effect on miR-1205 in CRC cells.
Upregulation of miR-1205 Was Answerable for the si-circRAD23B#2-Mediated Inhibition in CRC Development
Based on the transfection of si-circRAD23B#2, anti-miR-1205 was introduced into SW480 and SW620 cells with anti-miR-NC as the negative control for the mechanism analysis of circRAD23B. As the qRT-PCR data in Fig. 4a, b, the miR-1205 expression was decreased in si-circRAD23B#2 + anti-miR-1205 group contrasted with si-circRAD23B#2 + anti-miR-NC group, hinting that anti-miR-1205 transfection greatly inhibited miR-1205 in CRC cells. Subsequently, the si-circRAD23B#2-induced proliferation suppression (Fig. 4c, d), apoptosis promotion (Fig. 4e, f), and cell cycle arrest (Fig. 4g, h) were found to be countervailed by miR-1205 inhibitor. Similarly, the repressive effects on cell migration (Fig. 4i, j), invasion (Fig. 4k, l), and MMP2/MMP9 protein levels (Fig. 4m, n) triggered by si-circRAD23B#2 were also mitigated following the expression downregulation of miR-1205. Altogether, the downregulation of circRAD23B inhibited CRC progression via upregulating miR-1205.
MiR-1205 Negatively Regulated TRIM44 in CRC Cells
TRIM44 was shown to be upregulated in colon adenocarcinoma according to the dataset (Supplementary Fig. 2), and TargetScan software has presented the binding sites between miR-1205 and TRIM44 (Fig. 5a), implying the potential of TRIM44 as a target of miR-1205. Their interaction was verified using dual-luciferase reporter assay, in which only cotransfection of miR-1205 and TRIM44 3′UTR WT evoked the inhibition of luciferase activity (Fig. 5b, c). Pull-down assay also showed that the abundant TRIM44 was caught in Bio-miR-1205 group by contrast to Bio-miR-NC group (Fig. 5d, e). The qRT-PCR and western blot indicated that miR-1205 overexpression had inhibitory effect on the expression of TRIM44 in SW480 and SW620 cells (Fig. 5f, g). TRIM44 mRNA and protein levels were obviously elevated in CRC tissues (Fig. 5h, i) and cell (Fig. 5j, k) compared with their control groups. Therefore, TRIM44 was a downstream target of miR-1205 and was negatively regulated by miR-1205 in CRC cells.
MiR-1205 Downregulated TRIM44 to Function as a Tumor Inhibitor in CRC Cells
Transfection of miR-NC, miR-1205, miR-1205 + vector, and miR-1205 + TRIM44 was performed in SW480 and SW620 cells for the regulatory analysis between miR-1205 and TRIM44. Western blot exhibited that TRIM44 vector eliminated the inhibitory influence of miR-1205 on TRIM44 protein expression, affirming the great overexpression efficiency of TRMI44 transfection (Fig. 6a, b). Apparently, miR-1205 overexpression incurred the repression of proliferative capacity (Fig. 6c, d), acceleration of apoptotic generation (Fig. 6e, f), and the blockage of cell cycle progression (Fig. 6g, h), which were then ameliorated by the high TRIM44 expression in SW480 and SW620 cells. Also, TRIM44 upregulation restored the miR-1205-motivated suppressive effects on cell migration (Fig. 6i), invasion (Fig. 6j), and MMP2/MMP9 protein expression (Fig. 6k, l). As above described, miR-1205 played an inhibitory role in CRC via targeting TRIM44.
CircRAD23B Up-Modulated TRIM44 as a Sponge of miR-1205 in CRC Cells
To investigate whether circRAD23B could regulate TRIM44 via miR-1205, we transfected si-circRAD23B#2, si-circRAD23B#2 + anti-miR-1205, and the corresponding controls into SW480 and SW620 cells. The qRT-PCR analysis suggested that TRIM44 mRNA level was downregulated after transfection of si-circRAD23B#2, whereas there was a reversal for this downregulation by miR-1205 inhibitor (Fig. 7a, b). The same phenomenon was found in the protein expression of TRIM44 (Fig. 7c), again demonstrating the positive regulation of circRAD23B on TRIM44 by targeting miR-1205.
CRC Tumor Growth In Vivo Was Decelerated by circRAD23B Downregulation via the miR-1205/TRIM44 Network
After cell injection for 28 days in mice, tumor volume and weight of sh-circRAD23B group were exhibited to be declined in comparison with sh-NC group (Fig. 8a, b). The qRT-PCR and western blot were carried out for the expression analysis of three molecules in excised tumors. Sh-circRAD23B introduction reduced the circRAD23B level (Fig. 8c) and enhanced miR-1205 expression (Fig. 8d) in tumors relative to sh-NC, indicating that knockdown of circRAD23B increased the level of its target miR-1205. This downregulation of circRAD23B in vivo also repressed TRIM44 protein expression (Fig. 8e). CRC tumorigenesis in vivo was also suppressed by circRAD23B inhibition by acting on the miR-1205/TRIM44 axis.
Discussion
Recently, circRNAs have become a research emphasis in different kinds of cancers due to their multitudinous characteristics of abundance, stability, and conservation in cancer cells [16]. CircRNA dysregulation has pivotal applications in the biomedical development [17]. For instance, hsa_circRNA_102958 upregulation was affirmed as a diagnostic indication for gastric cancer [18], and hsa_circ_000449 knockdown or overexpression significantly affected cell migratory and invasive abilities in oral squamous cell carcinoma [19]. Luo et al. proposed that circPTPRM was overexpressed in hepatocellular carcinoma and generated the promoting effects on tumorigenesis and metastasis [20]. Hou et al. showed that circASS1 was downregulated in breast cancer and retarded cell migration and invasion [21]. Additionally, increasing studies have reported many tumor-regulatory circRNAs in CRC, such as tumorigenic circVAPA/circBANP [22, 23] and suppressive circDDX17/circITGA7 [24, 25].
In the current study, circRAD23B was identified as an upregulated molecule in CRC tissue samples and cells compared with the relative control tissues and cells. CircRAD23B was more stable than linear RAD23B after the treatment of RNase R in CRC cells. Our cellular experiments further demonstrated that the low expression of circRAD23B induced the inhibition of CRC cell proliferation, cell cycle progression, cell migration and invasion along with the increased cell apoptosis. These results supported that circRAD23B downregulation motivated the antitumor response in CRC developing process.
CircRNAs have the sponge effects on miRNAs via the molecular interaction [26,27,28]. Intriguingly, we found that circRAD23B exerted the negatively regulatory influences on miR-1205 in CRC cells. Bioinformatic analysis and dual-luciferase reporter assay revealed the binding between circRAD23B and miR-1205. Moreover, circRAD23B was suggested to act in CRC cells depending on its sponge function for miR-1205. MiR-1205 is a regulatory molecule with antitumor response in laryngeal squamous cell carcinoma via targeting E2F1 [29] and in glioma via inhibiting BATF3 [30]. In addition to the identification of CRKL as a downstream gene for miR-1205 in CRC [12], the other targets of miR-1205 remain to be discovered. Herein, miR-1205 was able to target TRIM44 and directly repressed the expression of TRIM44 in CRC cells. Moreover, the reverted assays revealed that miR-1205 played as an inhibitor in the progression of CRC by downregulating TRIM44.
TRIM44 has been considered as a carcinogen in many common cancers, including renal cell carcinoma [31], glioma [32], cervical cancer [33], and CRC [13]. Ji et al. asserted that long ncRNA DUXAP8 facilitated nonsmall cell lung cancer development via upregulating TRIM44 by modulating miR-498 [34]. Sun et al. clarified that LINC00265 activated the glycolytic process of CRC through the miR-216b-5p/TRIM44 axis [35]. The regulation of circRNA on TRIM44 is unreported in previous studies. In this chapter, circRAD23B was proved to regulate TRIM44 in a positive manner via targeting miR-1205, exhibiting the circRAD23B/miR-1205/TRIM44 signal axis in CRC. The further in vivo experiments again confirmed that the downregulation of circRAD23B repressed tumor growth via the modulation of miR-1205/TRIM44 axis.
Conclusion
Taken together, all these results manifested that knockdown of circRAD23B targeted the miR-1205/TRIM44 axis to suppress the progression of CRC. CircRAD23B can be used as a reliable target for CRC diagnosis, and circRAD23B inhibition may contribute to the therapeutic development of CRC as an effective antitumor option.
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10620_2021_6859_MOESM1_ESM.tif
Supplementary Fig.1. MiR-1205 was used as the target research for circRAD23B. (A) Venn diagram was performed to seek the potential targets for circRAD23B from the predicted miRNAs by circinteractome and circBank. (B) The expression levels of miR-1205, miR-1253, miR-892b and miR-935 were detected in SW480 cells transfected with si-NC or si-RAD23B#2. All experiments were performed three times (N=3). *P < 0.05. (TIF 365 kb)
10620_2021_6859_MOESM2_ESM.tif
Supplementary Fig.2. TRIM44 upregulation in colon adenocarcinoma. The dataset analysis of TRIM44 expression in 471 cancer and 41 normal samples. (TIF 367 kb)
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Han, B., Wang, X. & Yin, X. Knockdown of circRAD23B Exerts Antitumor Response in Colorectal Cancer via the Regulation of miR-1205/TRIM44 axis. Dig Dis Sci 67, 504–515 (2022). https://doi.org/10.1007/s10620-021-06859-w
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DOI: https://doi.org/10.1007/s10620-021-06859-w