Abstract
Fourteen polymorphic microsatellite loci in the endangered fern Adiantum reniforme var. sinense were developed and characterized using the Fast Isolation by AFLP of Sequences Containing repeats (FIASCO) protocol. Polymorphism of each locus was assessed in a bulked sample of 30 individuals from 8 natural populations. The number of alleles per locus varied from 2 to 11, with an average value of 6.2. The ranges of observed and expected heterozygosity were 0.000–0.895 and 0.226–0.868, respectively. These microsatellite markers provide useful tools for the ongoing conservation genetic studies of Adiantum reniforme var. sinense.
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References
Chen WL, Xie ZQ, Xiong GM (2004). Preliminary study on the ex-situ conservation of land plants in the Three Gorges Reservoir area. Resour. Environ. Yangtze Basin 13: 174–177
Doyle JJ, Doyle JL (1987). A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem. Bull. 19: 115
Fu LK, Jin JM (1992). China Plant Red Data Book – Rare and Endangered Plant. Science Press, Beijing.
Lin YX (1980) New taxa of Adiantum (Adiantaceae) from China. Acta Phytotaxon. Sinica 18: 101–105
Raymond M, Rousset F (1995). Genepop (Version 1.2): Population genetics software for exact tests and ecumenicism. J. Heredity 86: 248–249
Rozen S, Skaletsky HJ (2000). Primer3 on the www for general users and for biologist programmers. In: Krawetz S, Misener S (eds)., Bioinformatics Methods and Protocols: Methods in Molecular Biology. Humana Press, Totowa, NJ, USA, pp. 365–386
Shen GZ, Xie ZQ (2004). Three Gorge project: Chance and challenge. Science 304: 681
Xu HZ, Jin YX, Jiang MX, Wu JQ (1998). Spore propagation of Adiantum reniforme var.sinense endemic to Three-Gorge Reservoir Region. Resour. Environ. Yangtze Basin 7: 237–241
Zane L, Bargelloni L, Patarnello T (2002). Strategies for microsatellite isolation: A review. Mol. Ecol. 11: 1–16
Acknowledgements
This project was supported by National Natural Science Foundation of China (30470185), KIP Pilot of Chinese Academy of Sciences (No. KSXC2-SW-104) and WZ −050828 of Conservation Genetics Laboratory, WBG-CAS. We thank Dr Desmond Layne and Dr Andrew Lowe for reviewing the manuscript.
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Kang, M., Pan, L., Yao, X. et al. Development and characterization of polymorphic microsatellite loci in endangered fern Adiantum reniforme var. sinense . Conserv Genet 7, 807–810 (2006). https://doi.org/10.1007/s10592-005-9073-x
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DOI: https://doi.org/10.1007/s10592-005-9073-x