Abstract
Habitat fragmentation has resulted in many species becoming geographically restricted, as dispersal among subsequent isolates becomes compromised. This study investigated the effects of historical fragmentation on the genetic diversity and differentiation of Australia's Bunya Pine (Araucaria bidwillii) using random amplified DNA (RAPD) markers. High diversity characterises all Bunya populations sampled, regardless of population size or degree of isolation, which may be either due to similar effective population size among populations, or the lagging effects of the detection of contemporary genetic signal in long-lived conifer species. Large genetic differentiation characterises the northern and southern populations, although all sampled populations are significantly differentiated from each other. The northern population, at Mt Lewis, is responsible for the majority of variation detected among populations, and as such represents a significant genetic reservoir. The conservation of the genotypes of the Mt Lewis population may be imperative for the future of the species, given predicted models of accelerated climate change over the coming decades.
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Allnutt TR, Newton AC, Lara A, Premoli A, Armesto JJ, Vergara R, Gardner M (1999) Genetic variation in Fitzroya cupressoides (alerce), a threatened South American conifer. Mol. Ecol., 8, 975–987.
Allnutt TR, Courtis JR, Gardner M, Newton AC (2001) Genetic variation in wild Chilean and cultivated British popu-lations of Podocarpus salignus D. Don. (Podocarpaceae). Edinb. J. Bot., 58, 459–473.
Allnutt TR, Newton AC, Premoli A, Lara A (2003) Genetic variation in the threatened South American conifer Pilgerodendron uviferum (Cupressaceae), detected using RAPD markers. Biol. Conserv., 114, 245–253.
Bekessy SA, Allnutt TR, Premoli AC, Lara A, Ennos RA, Burgman MA, Cortes M, Newton AC (2002) Genetic variation in the Monkey Puzzle tree (Araucaria araucana (Molina) K. Koch), detected using RAPD. Heredity, 88, 243–249.
Bullock S, Summerall BA, Gunn LV (2000) Pathogenicity of ve fungi to the Wollemi pine, Wollemia nobilis. Australas. Plant Path., 29, 211–214.
Excoffier L, Smouse PE, Quattro JM (1992) Analysis of molecular variance inferred from metric distances among DNA haplotypes: Application to human mitochondrial DNA restriction data. Genetics, 131, 479–491.
Farjon A, Page CN (1999) Conifers-Status Survey and Conservation Action Plan. IUCN/SSC Conifer Specialist Group, Cambridge, UK.
Frankham R (1995) Conservation genetics. Annu. Rev. Genet., 29, 305–327.
Frankham R (1996) Relationship of genetic variation to pop-ulation size in wildlife. Conserv. Biol., 10, 1500–1508.
Gilpin ME, Soule ´ ME (1986) Minimum viable populations: Processes of species extinctions. In: Conservation Biology: The Science of Scarcity and Diversity. (Ed. Soule ´ ME), pp. 19–34. Sinauer Associates, Sunderland MA.
Gram WK, Sork VL (1999) Population density as a predictor of genetic variation for woody plant species. Conserv. Biol., 13 (5), 1079–1087.
Haebich A. (2002) On the Bunya Trail. Queensland Rev Special Edition, Volume 9(2), University of Queensland Press, Brisbane.
Hamrick JL, Godt MJW (1996) Conservation genetics of endemic plant species. In: Conservation Genetics: Case Histo-ries from Nature. (Eds. Avise JC, Hamrick JL), pp. 281–304. Chapman & Hall, New York.
Hopkins MS, Ash J, Graham AW, Head J, Hewett RK (1993) Charcoal evidence of the spatial extent of the Eucalyptus woodland expansions and rainforest contractions in North Queensland during the Late Pleistocene. J. Biogeogr., 20 (4), 357–372.
Houghton JT, Meira Filho LG, Callander BA, Harris N, Kattenberg A and Maskell K (Eds.) (1996) Climate Change 1995: The science of climate change. Cambridge University Press, Cambridge.
Huntley B (1998) The dynamic response of plants to environ-mental change and the resulting risks of extinction. In: Conservation in a Changing World. (Eds. Mace GM, Balmford A, Ginsberg JR), pp. 69–86. Cambridge University Press, Cambridge.
Kershaw AP, McGlone MS (1995) The Quaternary history of the southern conifers. In: Ecology of the Southern Conifers. (Eds. Enright NJ & Hill RS), pp. 30–63. Melbourne University Press, Melbourne.
Kershaw P, Wagsta. B (2001) The southern conifer family Araucariaceae: History, status, and value for paleoenvironmental reconstruction. Annu. Rev. Ecol. Syst., 32, 397–414.
Lewontin RC (1972) The apportionment of human diversity. Evol. Biol., 6, 381–398.
Loveless MD, Hamrick JL (1984) Ecological determinants of genetic structure in plant populations. Annu. Rev. Ecol. Syst., 15, 65–95.
Lynch M, Conery J, Bu ¨rger R (1995) Mutational meltdown in sexual populations. Evolution, 49, 1067–1080.
Meekins JF, Ballard HE Jr, McCarthy BC (2001) Genetic variation and molecular biogeography of a North American invasive plant species (Alliaria petiolata, Brassicaceae). Int. J. Plant Sci., 162 (1), 161–169.
Moritz C (2002) Strategies to protect biological diversity and the evolutionary processes that sustain it. Syst. Biol., 51 (2), 238–254.
Nei M (1973) Analysis of gene diversity in subdivided populations. P. Natl. Acad. Sci. USA, 70, 3321–3323.
Nybom H, Bartish IV (2000) Effects of life history traits and sampling strategies on genetic diversity estimates obtained with RAPD markers in plants. Perspect. Plant Ecol., 3 (2), 93–114.
Peakall R, Smouse PE (2001) GenAlEx V5: Genetic analysis in Excel. Population genetic software for teaching and research. Australian National University, Canberra, Australia. http://www. anu. edu. au/BoZo/GenAlEx/
Peakall R, Ebert D, Scott L, Meagher P, Offord C (2003) Comparative genetic study con rms exceptionally low ge-netic variation in the ancient and endangered relictual conifer, Wollemia nobilis (Araucariaceae). Mol. Ecol., 12, 2331–2343.
Petit RJ, El Mousadik A, Pons O (1998) Identifying populations for conservation on the basis of genetic markers. Conserv. Biol., 12 (4), 844–855.
Pye MG, Gadek PA, Edwards KJ (2003) Diversity, divergence and species of the Australasian Callitris (Cupressaceae) and allied genera: Evidence from ITS sequence data. Aust. Sys. Bot., 16 (4), 505–514.
Schneider S, Roessli D, Excoffier L (2000) Arlequin ver. 2. 000: A software for population genetics analysis. Genetics and Biometry Laboratory, University of Geneva, Switzerland.
Sherwin WB, Moritz C (2000) Managing and monitoring genetic erosion. In: Genetics, Demography and Viability of Fragmented Populations. (Eds. Young AG, Clarke GM), pp. 9–34. Cambridge University Press, Cambridge.
Stockey RA (1994) Mesozoic Araucariaceae: Morphology and systematic relationships. J. Plant Res., 107, 493–502.
Webb LJ, Tracey JG (1981) Australian rainforests: Patterns and change. In: Ecological Biogeography of Australia. Part 2: Flora (Ed. Keast A), pp. 605–694. Dr W. Junk Publishers, The Hague.
Williams JGK, Kubelik AR, Livak KJ, Rafalski JA, Tingey SV (1990) DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res., 18, 6531–6536.
Wu J, Krutovskii KV, Strauss SH (1999) Nuclear DNA diversity, population di. erentiation, and phylogenetic rela-tionships in the California closed-cone pines based on RAPD and allozyme markers. Genome, 42, 893–908.
Yeh FC, Yang R, Boyle T (1999) PopGene Ver. 1. 31. University of Alberta, Canada.
Young A, Boyle T, Brown T (1996) The population genetic consequences of habitat fragmentation for plants. Trends Ecol. Evol., 11 (10), 413–418.
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Pye, M.G., Gadek, P.A. Genetic diversity, differentiation and conservation in Araucaria bidwillii(Araucariaceae), Australia's Bunya pine. Conservation Genetics 5, 619–629 (2004). https://doi.org/10.1007/s10592-003-1857-2
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DOI: https://doi.org/10.1007/s10592-003-1857-2