Abstract
The viscoelastic and inertial properties of the arterial wall are responsible for the arterial functional role in the cardiovascular system. Cryopreservation is widely used to preserve blood vessels for vascular reconstruction but it is controversially suspected to affect the dynamic behaviour of these allografts. The aim of this work was to assess the cryopreservation's effects on human arteries mechanical properties. Common carotid artery (CCA) segments harvested from donors were divided into two groups: Fresh (n = 18), tested for 24–48 h after harvesting, and Cryopreserved (n = 18) for an average time of 30 days in gas-nitrogen phase, and finally defrosted. Each segment was tested in a circulation mock, and its pressure and diameter were registered at similar pump frequency, pulse and mean pressure levels, including those of normotensive and hipertensive conditions. A compliance transfer function (diameter/pressure) derived from a mathematical adaptive modelling was designed for the on line assessment of the arterial wall dynamics and its frequency response. Assessment of arterial wall dynamics was made by measuring its viscous (η), inertial (M) and elastic (E) properties, and creep and stress relaxation time constant (τC and τSR, respectively). The frequency response characterization allowed to evaluate the arterial wall filter or buffer function. Results showed that non-significant differences exist between wall dynamics and buffer function of fresh and cryopreserved segments of human CCA. In conclusion, our cryopreservation method maintains arterial wall functional properties, close to their fresh values.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
Abbreviations
- BF:
-
arterial buffering function
- CF:
-
arterial conduit function
- ARX:
-
autoregressive with exogenous input model
- CCA:
-
common carotid arteries
- τC :
-
creep time constant
- f C :
-
cut-off frequency
- E :
-
elastic index
- ECMM:
-
esternal cleido mastoid muscle
- M :
-
inertial index
- τSR :
-
stress relaxation time constant
- η :
-
viscous index
References
M. Adham J.P. Gournier J.P. Favre E. La Roche C. Ducerf J. Baulieux X. Barral M. Pouyet (1996) ArticleTitleMechanical characteristics of fresh and frozen human descending thoracic aorta J. Surg. Res. 15 64 IssueID1 32–34 Occurrence Handle1:STN:280:DyaK28vgtVSqtg%3D%3D Occurrence Handle10.1006/jsre.1996.0302
H. Akaike (1969) ArticleTitleFitting autoregressive models for prediction Ann. Inst. Stat. Math. 21 243–247
I. Alvarez M. DelCarmen Saldias O. Wodowoz H. Perez Campos D. Machin W. Silva P. Sueta N. Perez C. AcostaMd Mdel (2003) ArticleTitleProgress of national multi-tissue bank in uruguay in the international atomic energy agency (IAEA) tissue banking programme Cell Tissue Bank 4 IssueID2–4 173–178 Occurrence Handle15256857 Occurrence Handle10.1023/B:CATB.0000007036.45040.20
R.L. Armentano J.G. Barra J. Levenson A. Simon R.H. Pichel InstitutionalAuthorNameArterial wall mechanics in conscious dogs (1995a) ArticleTitleAssessment of viscous, inertial, and elastic moduli to characterize aortic wall behavior Circ. Res. 76 IssueID3 468–478 Occurrence Handle1:CAS:528:DyaK2MXkt1Wrsr8%3D
R. Armentano J.L. Megnien A. Simon F. Bellenfant J. Barra J. Levenson (1995b) ArticleTitleEffects of hypertension on viscoelasticity of carotid and femoral arteries in humans Hypertension 26 IssueID1 48–54 Occurrence Handle1:STN:280:DyaK2MzjtFymug%3D%3D
R.L. Armentano D. Bia D. Craiem L. Gamero J. Levenson J.C. Grignola F.F. Ginés (2003) ArticleTitleRespuesta en frecuencia de la pared arterial: inocente o culpable de las discrepancias entre filtrado sistémico y pulmonar? Rev. Mex. Ing. Biom. 24 IssueID1 45–54
D. Bia R.L. Armentano J.C. Grignola D. Craiem Y.A. Zocalo F.F. Gines J Levenson (2003) ArticleTitleThe vascular smooth muscle of great arteries: local control site of arterial buffering function? Rev. Esp. Cardiol. 56 IssueID12 1202–1209 Occurrence Handle14670273 Occurrence Handle10.1157/13055339
D. Bia R. Armentano D. Craiem J. Grignola F. Gines A. Simon J. Levenson (2004) ArticleTitleSmooth muscle role on pulmonary arterial function during acute pulmonary hypertension in sheep Acta Physiol. Scand 181 IssueID3 359–366 Occurrence Handle15196096 Occurrence Handle1:CAS:528:DC%2BD2cXlslSqtrw%3D Occurrence Handle10.1111/j.1365-201X.2004.01294.x
Bia Santana D., Barra J.G., Grignola J.C., Gines F.F. and Armentano R.L. 2005. Pulmonary artery smooth muscle activation attenuates arterial dysfunction during acute pulmonary hypertension. J Appl Physiol. Feb; 98(2): 605–613. doi:1011152/jappliedphysiol.00361.2004]. Epub 2004, Oct. 15.
W. Blondel B. Lehalle G. Maurice X. Wang J.F. Stoltz (2000) ArticleTitleRheological properties of fresh and cryopreserved human arteries tested in vitro Rheol. Acta 39 IssueID5 461–468 Occurrence Handle1:CAS:528:DC%2BD3cXotV2mt7o%3D Occurrence Handle10.1007/s003970000110
J. Bujan G. Pascual R. Lopez C. Corrales M. Rodriguez F. Turegano J.M. Bellon (2001) ArticleTitleGradual thawing improves the preservation of cryopreserved arteries Cryobiology 42 IssueID4 256–265 Occurrence Handle11748934 Occurrence Handle1:STN:280:DC%2BD38%2Fgt1Gnuw%3D%3D Occurrence Handle10.1006/cryo.2001.2329
R. Dawber (1988) ArticleTitleCold kills! Clin. Exp. Dermatol. 13 IssueID3 137–150 Occurrence Handle3073026 Occurrence Handle1:STN:280:DyaL1M3jtVOqsw%3D%3D Occurrence Handle10.1111/j.1365-2230.1988.tb01958.x
E.I. Fischer R.L. Armentano F.M. Pessana S. Graf L. Romero A.I. Christen A. Simon J. Levenson (2002) ArticleTitleEndothelium-dependent arterial wall tone elasticity modulated by blood viscosity Am. J. Physiol. Heart Circ. Physiol. 282 IssueID2 H389–H394 Occurrence Handle11788384 Occurrence Handle1:CAS:528:DC%2BD38XhtFCqsLo%3D
G.M. Fahy T.H. Lilley H. Linsdell M.S. Douglas H.T. Meryman (1990) ArticleTitleCryoprotectant toxicity and cryoprotectant toxicity reduction: in search of molecular mechanisms Cryobiology 27 IssueID3 247–268 Occurrence Handle2199153 Occurrence Handle1:CAS:528:DyaK3cXkvFChur8%3D Occurrence Handle10.1016/0011-2240(90)90025-Y
G.M. Fahy D.I. Levy S.E. Ali (1987) ArticleTitleSome emerging principles underlying the physical properties, biological actions, and utility of vitrification solutions Cryobiology 24 IssueID3 196–213 Occurrence Handle3595164 Occurrence Handle1:CAS:528:DyaL2sXkslekt7Y%3D Occurrence Handle10.1016/0011-2240(87)90023-X
L.G. Gamero R.L. Armentano J.G. Barra A. Simon J. Levenson (2001) ArticleTitleIdentification of arterial wall dynamics in conscious dogs Exp. Physiol. 86 IssueID4 519–528 Occurrence Handle11445831 Occurrence Handle1:STN:280:DC%2BD38%2FivVWrsQ%3D%3D Occurrence Handle10.1113/eph8602172
J.P. Gournier M. Adham J.P. Favre M. Raba B. Bancel J.C. Lepetit X. Barral (1993) ArticleTitleCryopreserved arterial homografts: preliminary study Ann. Vasc. Surg. 7 IssueID6 503–511 Occurrence Handle8123452 Occurrence Handle1:STN:280:DyaK2c7mvV2htQ%3D%3D Occurrence Handle10.1007/BF02000144
C.J. Hunt Y.C. Song E.A. Bateson D.E. Pegg (1994) ArticleTitleFractures in cryopreserved arteries Cryobiology 31 IssueID5 506–515 Occurrence Handle7988160 Occurrence Handle1:STN:280:DyaK2M%2FnvFOktg%3D%3D Occurrence Handle10.1006/cryo.1994.1061
J.O. Karlsson M. Toner (1996) ArticleTitleLong-term storage of tissues by cryopreservation: critical issues Biomaterials 17 IssueID3 243–256 Occurrence Handle8745321 Occurrence Handle1:CAS:528:DyaK28XovVSnsQ%3D%3D Occurrence Handle10.1016/0142-9612(96)85562-1
S.E. Langerak M. Groenink E.E. der Wall C. Wassenaar E. Vanbavel M.C. Baal Particlevan J.A. Spaan (2001) ArticleTitleImpact of current cryopreservation procedures on mechanical and functional properties of human aortic homografts Transpl. Int. 14 IssueID4 248–255 Occurrence Handle11512058 Occurrence Handle1:STN:280:DC%2BD3MvmslWntg%3D%3D Occurrence Handle10.1111/j.1432-2277.2001.tb00053.x
B. Lehalle C. Geschier G. Fieve J.F. Stoltz (1997) ArticleTitleEarly rupture and degeneration of cryopreserved arterial allografts J. Vasc. Surg. 25 IssueID4 751–2 Occurrence Handle9129634 Occurrence Handle1:STN:280:DyaK2s3nsFyksQ%3D%3D Occurrence Handle10.1016/S0741-5214(97)70305-7
L. Ljung (1999) System Identification – Theory for the User EditionNumber2 Upper Saddle River NJ, Prentice Hall
G. Pascual F. Jurado M. Rodriguez C. Corrales P. Lopez-Hervas J.M. Bellon J. Bujan (2002) ArticleTitleThe use of ischaemic vessels as prostheses or tissue engineering scaffolds after cryopreservation Eur. J. Vasc. Endovasc. Surg. 24 IssueID1 23–30 Occurrence Handle12127844 Occurrence Handle1:STN:280:DC%2BD38zotFyrtg%3D%3D Occurrence Handle10.1053/ejvs.2002.1663
D.E. Pegg M.C. Wusteman S. Boylan (1997) ArticleTitleFractures in cryopreserved elastic arteries Cryobiology 34 IssueID2 183–192 Occurrence Handle9130389 Occurrence Handle1:STN:280:DyaK2s3ns1KlsA%3D%3D Occurrence Handle10.1006/cryo.1996.1997
L.H. Peterson R.E. Jensen J. Parnell (1960) ArticleTitleMechanical properties of arteries in vivo Circ. Res. 8 622–639
G. Pontrelli E. Rossoni (2003) ArticleTitleNumerical modelling of the pressure wave propagation in the arterial flow Int. J. Numer. Meth. Fluids 43 651–671 Occurrence Handle10.1002/fld.494
F. Pukacki T. Jankowski M. Gabriel G. Oszkinis Z. Krasinski S. Zapalski (2000) ArticleTitleThe mechanical properties of fresh and cryopreserved arterial homografts Eur. J. Vasc. Endovasc. Surg. 20 IssueID1 21–24 Occurrence Handle10906292 Occurrence Handle1:STN:280:DC%2BD3cvgtF2gug%3D%3D Occurrence Handle10.1053/ejvs.2000.1120
M.E. Rendal Vazquez M. Rodriguez Cabarcos M.V. Martinez Santos R.O. Fernandez Mallo J. Sanchez Ibanez R. Segura Iglesias G. Matheu Capo P. Filgueira Fernandez S. Pertega Diaz T. Bermudez Gonzalez C. Andion Nunez (2004) ArticleTitleFunctional assessment of cryopreserved human aortas for pharmaceutical research Cell Tissue Bank 5 IssueID2 119–123 Occurrence Handle15241008 Occurrence Handle10.1023/B:CATB.0000034087.26468.5a
M. Rigol M. Heras A. Martinez M.J. Zurbano E. Agusti E. Roig J.L. Pomar G. Sanz (2000) ArticleTitleChanges in the cooling rate and medium improve the vascular function in cryopreserved porcine femoral arteries J. Vasc. Surg. 31 IssueID5 1018–1025 Occurrence Handle10805894 Occurrence Handle1:STN:280:DC%2BD3c3ms1agug%3D%3D Occurrence Handle10.1067/mva.2000.103793
A.M. Seifalian A. Tiwari G. Hamilton H.J. Salacinski (2002) ArticleTitleImproving the clinical patency of prosthetic vascular and coronary bypass grafts: the role of seeding and tissue engineering Artif. Organs 26 IssueID4 307–320 Occurrence Handle11952502 Occurrence Handle10.1046/j.1525-1594.2002.06841.x
J.P. Shepherd R.P. Dawber (1982) ArticleTitleThe response of keloid scars to cryosurgery Plast Reconstr. Surg. 70 IssueID6 677–682 Occurrence Handle7146149 Occurrence Handle1:STN:280:DyaL3s%2FmsFOisA%3D%3D Occurrence Handle10.1097/00006534-198212000-00003
J.P. Shepherd R.P. Dawber (1984) ArticleTitleWound healing and scarring after cryosurgery Cryobiology 21 IssueID2 157–169 Occurrence Handle6713945 Occurrence Handle1:STN:280:DyaL2c7otFOksA%3D%3D Occurrence Handle10.1016/0011-2240(84)90207-4
N.R. Tai A. Giudiceandrea H.J. Salacinski A.M. Seifalian G. Hamilton (1999) ArticleTitleIn vivo femoropopliteal arterial wall compliance in subjects with and without lower limb vascular disease J Vasc Surg 30 IssueID5 936–945 Occurrence Handle10550193 Occurrence Handle1:STN:280:DC%2BD3c%2FhvVeisg%3D%3D Occurrence Handle10.1016/S0741-5214(99)70020-0
N.R. Tai H.J. Salacinski A. Edwards G. Hamilton A.M. Seifalian (2000) ArticleTitleCompliance properties of conduits used in vascular reconstruction Br. J. Surg. 87 IssueID11 1516–1524 Occurrence Handle11091239 Occurrence Handle1:STN:280:DC%2BD3M%2FltFOitQ%3D%3D Occurrence Handle10.1046/j.1365-2168.2000.01566.x
M. Vischjager T.M. Gulik ParticleVan J. Marle ParticleVan M. Pfaffendorf M.J. Jacobs (1996) ArticleTitleFunction of cryopreserved arterial allografts under immunosuppressive protection with cyclosporine A J. Vasc. Surg. 24 IssueID5 876–882 Occurrence Handle8918337 Occurrence Handle1:STN:280:DyaK2s%2Fns1Sitg%3D%3D Occurrence Handle10.1016/S0741-5214(96)70026-5
C. Wassenaar E.G. Wijsmuller L.A. Herwerden ParticleVan Z. Aghai C.L. Tricht ParticleVan E. Bos (1995) ArticleTitleCracks in cryopreserved aortic allografts and rapid thawing Ann. Thorac. Surg. 60 IssueID2 Suppl S165–S167 Occurrence Handle7646151 Occurrence Handle1:STN:280:DyaK2MznsVWgtQ%3D%3D Occurrence Handle10.1016/0003-4975(95)00264-L
S.M. Wells B.L. Langille J.M. Lee S.L. Adamson (1999) ArticleTitleDeterminants of mechanical properties in the developing ovine thoracic aorta Am. J. Physiol. 277 IssueID4 Pt 2 H1385–H1391 Occurrence Handle10516173 Occurrence Handle1:CAS:528:DyaK1MXntF2htLc%3D
N. Westerhof A. Noordergraaf (1970) ArticleTitleArterial viscoelasticity: a generalized model. Effect on input impedance and wave travel in the systematic tree J. Biomech. 3 357–379 Occurrence Handle5521552 Occurrence Handle1:STN:280:DyaE38%2FivVOltg%3D%3D Occurrence Handle10.1016/0021-9290(70)90036-9
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Bia, D., Pessana, F., Armentano, R. et al. Cryopreservation procedure does not modify human carotid homografts mechanical properties: an isobaric and dynamic analysis. Cell Tissue Banking 7, 183–194 (2006). https://doi.org/10.1007/s10561-005-0655-0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/s10561-005-0655-0