Abstract
We have developed an integrated hydrogenated amorphous silicon (a-Si:H) fluorescence detector for microfluidic genetic analysis. It consists of a half-ball lens, a ZnS/YF3 multilayer optical interference filter with a pinhole, and an annular a-Si:H PIN photodiode allowing the laser excitation to pass up through the central aperture in the photodiode and the filter. Microfluidic separations of multiplex PCR products generated from methicillin-resistant/sensitive Staphylococcus aureus (MRSA/MSSA) DNA on microfluidic capillary electrophoresis (CE) devices are successfully detected with the integrated detector. Similarly, multiplex PCR amplicons from the kanamycin resistant and K12 serotype-specific genes of E. coli cells are detected. The direct detection of multiplex PCR amplicons indicates that the fluorescence detector can be successfully coupled with current microfluidic PCR-CE platforms. This work establishes that the integrated a-Si:H detector provides relevant limits of detection for point-of-care genetic and pathogen analysis with microfluidic devices.
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M.L. Chabinyc, D.T. Chiu, J.C. McDonald, A.D. Stroock, J.F. Christian, A.M. Karger, and G.M. Whitesides, Anal. Chem. 73, 4491 (2001).
C.A. Emrich, H.J. Tian, I.L. Medintz, and R.A. Mathies, Anal. Chem. 74, 5076 (2002).
S. Hjertén, J. Chromatogr. 347, 191 (1985).
T. Kamei, B.M. Paegel, J.R. Scherer, A.M. Skelley, R.A. Street, and R.A. Mathies, Anal. Chem. 75, 5300 (2003).
E.T. Lagally, J.R. Scherer, R.G. Blazej, N.M. Toriello, B.A. Diep, M. Ramchandani, G.F. Sensabaugh, L.W. Riley, and R.A. Mathies, Anal. Chem. 76, 3162 (2004).
E.T. Lagally, P.C. Simpson, and R.A. Mathies, Sens. Actuator B-Chem. 63, 138 (2000).
M.A. McClain, C.T. Culbertson, S.C. Jacobson, N.L. Allbritton, C.E. Sims, and J.M. Ramsey, Anal. Chem. 75, 5646 (2003).
B.M. Paegel, C.A. Emrich, G.J. Weyemayer, J.R. Scherer, and R.A. Mathies, Proc. Natl. Acad. Sci. U.S.A. 99, 574 (2002).
B.M. Paegel, S.H.I. Yeung, and R.A. Mathies, Anal. Chem. 74, 5092 (2002).
P.C. Simpson, D. Roach, A.T. Woolley, T. Thorsen, R. Johnston, G.F. Sensabaugh, and R.A. Mathies, Proc. Natl. Acad. Sci. U.S.A. 95, 2256 (1998).
P. Vannuffel, P.F. Laterre, M. Bouyer, J. Gigi, B. Vandercam, M. Reynaert, and J.L. Gala, Journal of Clinical Microbiology 36, 2366 (1998).
J.R. Webster, M.A. Burns, D.T. Burke, and C.H. Mastrangelo, Anal. Chem. 73, 1622 (2001).
A.T. Woolley, D. Hadley, P. Landre, A.J. deMello, R.A. Mathies, and M.A. Northrup, Anal. Chem. 68, 4081 (1996).
A.T. Woolley and R.A. Mathies, Proc. Natl. Acad. Sci. U.S.A. 91, 11348 (1994).
A.T. Woolley and R.A. Mathies, Anal. Chem. 67, 3676 (1995).
G.M. Yee, N.I. Maluf, G.T.A. Kovacs, P.A. Hing, and M. Albin, Sensors and Actuators A-Physical 58, 61 (1997).
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Kamei, T., Toriello, N.M., Lagally, E.T. et al. Microfluidic Genetic Analysis with an Integrated a-Si:H Detector. Biomed Microdevices 7, 147–152 (2005). https://doi.org/10.1007/s10544-005-1595-y
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DOI: https://doi.org/10.1007/s10544-005-1595-y