Abstract
Two extracellular oxidases (laccases) were isolated from the extracellular fluid of the fungus Panus (Lentinus) tigrinus cultivated in low-nitrogen medium supplemented with birch sawdust. The enzymes were purified by successive chromatography on columns with TEAE-cellulose and DEAE-Toyopearl 650M. Both oxidases catalyze oxidation of pyrocatechol and ABTS. Moreover, oxidase 1 also catalyzes oxidation of guaiacol, o-phenylenediamine, and syringaldazine. The enzymes have identical pH (7.0) and temperature (60–65°C) optimums. Absorption spectra of the oxidases differ from the spectra of typical “blue” laccases and are similar to the spectrum of yellow oxidase.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
REFERENCES
Kirk, T. K., and Farrell, R. L. (1987) Ann. Rev. Microbiol., 71, 465–505.
Eriksson, K. E. L., Blanchette, R. A., and Ander, P. (1990) Microbial and Enzymatic Degradation of Wood and Wood Components, Springer, Berlin-Heidelberg-New York.
Abadulla, E., Tranou, T., Costa, S., Robra, K. H., Cavaco, P. A., and Gulbitz, G. M. (2000) Appl. Environ. Microbiol., 66, 3357–3362.
Mai, C., Milstein, O., and Huttermann, A. (2000) J. Biotechnol., 79, 173–183.
Cadimaliev, D. A., Revin, V. V., and Shutova, V. V. (2001) Khim. Rast. Syr’ya, 3, 111–118.
Revin, V. V., Cadimaliev, D. A., Shutova, V. V., and Samuilov, V. D. (2002) Prikl. Biokhim. Mikrobiol., 38, 529–533.
Revin, V. V., Cadimaliev, D. A., Atykyan, N. A., Sitkin, B. V., and Samuilov, V. D. (2000) Biochemistry (Moscow), 65, 1305–1309.
Pozdnyakova, N. N., Leontievsky, A. A., and Golovleva, L. A. (1999) Biochemistry (Moscow), 64, 442–447.
Leontievsky, A. A., Pozdnyakova, N. N., Myasoedova, N. M., and Golovleva, A. A. (1996) Biochemistry (Moscow), 61, 1262–1268.
Revin, V. V., Prytkova, T. N., Liyas’kina, E. V., Cherkasov, V. D., and Solomatov, V. I. The Certificate on Deposition of the Microorganism Panus (Lentinus) tigrinus (Bulliard Fries) Fries, 317. No. BKM F-3616D, 05.03.98.
Eggert, C., Temp, U., and Eriksson, K.-E. L. (1996) Appl. Environ. Microbiol., 62, 1151–1158.
Sinitsyn, A. P., Chernoglazov, V. P., and Gusakov, A. V. (1990) Advances in Science and Technology. Biotechnology [in Russian], Vol. 25, VINITI, Moscow, pp. 80–93.
Bradford, M. M. (1976) Analyt. Biochem., 72, 248–254.
Gorbatova, O. N., Stepanova, O. N., and Koroleva, O. V. (2000) Prikl. Biokhim. Mikrobiol., 36, 272–277.
Munoz, C., Guillen, F., Martinez, A. T., and Martinez, M. J. (1997) Appl. Environ. Microbiol., 63, 2166–2174.
Gazaryan, I. G., Reshetnikova, I. A., Doseeva, V. V., and Bekker, E. G. (1995) Biochemistry (Moscow), 60, 767–772.
Harkin, J. M., and Obst, J. R. (1973) Experientia, 29, 381–385.
Dawson, J. H., Dooley, D. M., Clark, R., Stephens, P. J., and Gray, H. B. (1979) J. Amer. Chem. Soc., 101, 5046–5053.
Brown, J. M., Powers, L., Kinacaid, B., Larrabee, J. A., and Spiro, T. G. (1980) J. Amer. Chem. Soc., 102, 4210–4216.
Beltramini, M., Selvato, B., Santamaria, M., and Lerch, K. (1990) Biochim. Biophys. Acta, 1040, 365–372.
Koroleva, O. V., Stepanova, E. V., Gavrilova, V. P., Dzhafarova, A. N., Lyubimova, N. V., and Yaropolov, A. I. (1998) Prikl. Biokhim. Mikrobiol., 34, 490–494.
Solomon, E. I., Sundaram, U. M., and Machonkin, T. E. (1996) Chem. Rev., 96, 2563–2605.
Leontievsky, A. A. (2002) Ligninases of Basidiomycetes: Author’s abstract of doctoral dissertation (Biology) [in Russian], Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino.
Mayer, A. M., and Staples, R. C. (2002) Phytochem., 60, 551–565.
Leontievsky, A. A., Vares, T., Lankinen, P., Shergill, J. K., Pozdnyakova, N. N., Myasedova, N. M., Kalkkinen, N., Golovleva, L. A., Cammack, R., Thurtson, C. F., and Hatakka, A. (1997) FEMS Microbiol. Lett., 156, 9–14.
Palmieri, G., Giardina, P., Blanco, C., Scaloni, A., Capasso, A., and Sannia, G. (1997) J. Biol. Chem., 272, 31301–31307.
Author information
Authors and Affiliations
Corresponding author
Additional information
__________
Translated from Biokhimiya, Vol. 70, No. 6, 2005, pp. 850–854.
Original Russian Text Copyright © 2005 by Cadimaliev, Revin, Atykyan, Samuilov.
Rights and permissions
About this article
Cite this article
Cadimaliev, D.A., Revin, V.V., Atykyan, N.A. et al. Extracellular Oxidases of the Lignin-Degrading Fungus Panus tigrinus . Biochemistry (Moscow) 70, 703–707 (2005). https://doi.org/10.1007/s10541-005-0171-7
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/s10541-005-0171-7