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Genome Re-duplication and Irregular Segregation Occur During the Cell Cycle of Entamoeba histolytica

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Abstract

Heterogeneity of genome content is commonly observed in axenic cultures of Entamoeba histolytica. Cells with multiple nuclei and nuclei with heterogenous genome contents suggest that regulatory mechanisms that ensure alternation of DNA synthesis and mitosis are absent in this organism. Therefore, several endo-reduplicative cycles may occur without mitosis. The data also shows that unlike other endo-reduplicating organisms, E.histolytica does not undergo a precise number of endo-reduplicative cycles. We propose that irregular endo-reduplication and genome partitioning lead to heterogeneity in the genome content of E.histolytica trophozoites in their proliferative phase. The goal of future studies should be aimed at understanding the mechanisms that are involved in (a) accumulation of multiple genome contents in a single nucleus; (b) genome segregation in nuclei that contain multiple genome contents and (c) maintenance of genome fidelity in E. histolytica.

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Acknowledgments

This study was supported by FIRCA sub-grants from University of Virginia (GC11289-123722; Primary award number 1RO3TW007314) and Stanford University (16846170-33918-A; Primary award number 1RO3TW007421-01).

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Authors and Affiliations

  1. Department of Biochemistry, Bose Institute, Kolkata, 700054, India

    Anuradha Lohia, Chandrama Mukherjee, Shubhra Majumder & Promita Ghosh Dastidar

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  1. Anuradha Lohia
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  2. Chandrama Mukherjee
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  3. Shubhra Majumder
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  4. Promita Ghosh Dastidar
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Correspondence to Anuradha Lohia.

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Lohia, A., Mukherjee, C., Majumder, S. et al. Genome Re-duplication and Irregular Segregation Occur During the Cell Cycle of Entamoeba histolytica . Biosci Rep 27, 373–384 (2007). https://doi.org/10.1007/s10540-007-9058-8

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  • DOI: https://doi.org/10.1007/s10540-007-9058-8

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