Abstract
Iron is required for the Fe-S cluster assembly which occurs in chloroplasts, mitochondria, and cytosol and here we characterized 44 Fe-S cluster biosynthesis genes and investigated their expression profiles during different peach flowering stages. Quantitative real-time PCR analysis shows that the highest expression of most peach Fe-S cluster biosynthesis genes appeared in the full bloom stage. Also, the highest Fe accumulation occurred in the full bloom stage followed by beginning bloom, petal fall, and bud swell stages. Activities of nitrite reductase (NiR) and succinate dehydrogenase (SDH) were closely correlated to the flower Fe content, whereas the aconitase (ACO) activity kept steady during the whole flowering process. Moreover, shading treatment significantly reduced Fe accumulation and NiR, SDH, and ACO activities of the full blooming flowers. Seventeen Fe-S cluster biosynthesis genes were down-regulated in response to a shading treatment. In particular, plastid sulfur mobilization genes were sensitive to the shading treatment.
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Abbreviations
- ACO:
-
aconitase
- CIA:
-
cytosolic iron-sulfur cluster assembly
- FS:
-
flowering stage
- ICP-AES:
-
inductively coupled plasma atomatic emission spectrometry
- ISC:
-
iron-sulfur cluster
- NiR:
-
nitrite reductase
- RT-qPCR:
-
real-time quantitative PCR
- SDH:
-
succinate dehydrogenase
- SUF:
-
sulfur mobilization
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Acknowledgments: This work was supported by grants from the National Natural Science Foundations of China (31501743), the China Agriculture Research System (CARS-31), the China Postdoctoral Science Foundation (2015M571713), and the Jiangsu Agriculture Science and Technology Innovation Fund (CX(14)2015).
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Song, Z.Z., Zhang, B.B., Zhang, C.H. et al. Differential expression of iron-sulfur cluster biosynthesis genes during peach flowering. Biol Plant 60, 79–85 (2016). https://doi.org/10.1007/s10535-015-0577-z
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DOI: https://doi.org/10.1007/s10535-015-0577-z