Abstract
Peroxiredoxins (Prxs) play major roles in preventing oxidative damage and their function is consistent with the presence of Prx isoforms in most, if not all, cellular compartments and their expression is dependent on environmental conditions. The aim of this study was to identify and characterize genes encoding Prxs in Vitis vinifera. Quantitative real time polymerase chain reaction (qRT PCR) was used to determine their response to irradiance, heat, and water stress. We identified seven vvprx genes, two of which were especially responsive to water stress, followed by heat stress, but no major changes were observed after high irradiance. The vvprxIIF targeted to mitochondria was the most responsive to water stress and it might be involved in drought tolerance through H2O2 signalling. The vvprxII-2, a putative PrxII, is targeted to the chloroplasts and was the most responsive to heat stress. It might be related with abscisic acid-dependent thermotolerance. Additional key words: abscisic acid, antioxidants, grapevine, heat stress, photosynthesis, qRT PCR, water stress.
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Abbreviations
- ABA:
-
abscisic acid
- APX:
-
ascorbate peroxidase
- CAT:
-
catalase
- cys:
-
cystein
- DHAR:
-
dihydroascorbate reductase
- Fv/Fm :
-
variable to maximum chlorophyll fluorescence ratio
- GOR:
-
glutathione reductase
- PPDF:
-
photosynthetic photon flux density
- Prx:
-
peroxiredoxin
- qRT-PCR:
-
quantitative real time polymerase chain reaction
- ROS:
-
reactive oxygen species
- SOD:
-
superoxide dismutase
- WS:
-
water stress
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Acknowledgements: This research work was supported by project PTDC/AGR-GPL/099624/2008; CBAA (PestOE/AGR/ UI0240/2011) and by the FCT-awarded postdoctoral fellowship SFRH/BPD/43898/2008 to PV.
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Vidigal, P., Carvalho, R., Amâncio, S. et al. Peroxiredoxins are involved in two independent signalling pathways in the abiotic stress protection in Vitis vinifera . Biol Plant 57, 675–683 (2013). https://doi.org/10.1007/s10535-013-0346-9
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DOI: https://doi.org/10.1007/s10535-013-0346-9