Abstract
In this work, we report the ectopic expression of the Osmyb4 rice gene, encoding the Myb4 transcription factor, in Nicotiana tabacum and Salvia sclarea. Transcriptional analysis of T2 homozygous tobacco plants overexpressing Osmyb4 revealed that Myb4 activated the transcription of several genes of the phenylpropanoid pathway such as PAL, C4H, 4CL1, 4CL2 (encoding phenylalanine ammonia-lyase, cinnamic acid 4-hydroxylase, 4-coumarate: Co A ligase1, 4-coumarate: Co A ligase2). Moreover, the Myb4 increased expression of HQT encoding hydroxycinnamoyl-CoA: quinate transferase, which specifically triggers the accumulation of chlorogenic acid (CGA). In addition, increased acccumulation of rosmarinic acid (RA) was found in transgenic plants of both species. These results open the possibility of using the Osmyb4 gene to increase the production of specific bioactive hydroxycinnamates.
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Abbreviations
- 4CL:
-
4-coumarate:CoA ligase
- CGA:
-
chlorogenic acid
- C4H:
-
cinnamic acid 4-hydroxylase
- EPSPS:
-
5-enolpyruvylshikimate 3-phosphate synthase
- EV:
-
empty vector
- HPLC-DAD:
-
high performance liquid chromatography — with diode array detection
- HQT:
-
hydroxycinnamoyl CoA quinate transferase
- PAL:
-
phenylalanine ammonia lyase
- RA:
-
rosmarinic acid
- RAS:
-
RA synthase
- RT-PCR:
-
reverse transcription — polymerase chain reaction
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Acknowledgements: This work was supported by the MIUR-PRIN Project 2005 “Metabolic engineering of bioactive secondary metabolites in Nicotiana tabacum and Salvia sclarea” to A.L.
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Docimo, T., Mattana, M., Fasano, R. et al. Ectopic expression of the Osmyb4 rice gene enhances synthesis of hydroxycinnamic acid derivatives in tobacco and clary sage. Biol Plant 57, 179–183 (2013). https://doi.org/10.1007/s10535-012-0257-1
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DOI: https://doi.org/10.1007/s10535-012-0257-1