Abstract
Induction of somatic embryogenesis in leaf explants from young mangosteen seedlings using different concentrations and combinations of 6-benzylaminopurine (BAP) and thidiazuron (TDZ) was investigated. The best medium inducing the formation of globular structures (40 %) was Murashige and Skoog medium with 0.7 mg dm−3 BAP and 0.7 mg dm−3 TDZ. For their further development, subculturing onto different maturation media was carried out, but these globular structures did not develop futher stages of somatic embryogenesis. However, they developed shoots after 90 d of culture on the original medium. Morphological and histological analyses were performed, and showed that the globular structures resembled closely the undifferentiated structure of the mangosteen seed. We propose that the development of mangosteen somatic embryos does not follow the typical course of somatic embryogenesis, but the course of development that is natural for mangosteen seed, where procambium is the only structure observed and there is no differentiated embryo.
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Abbreviations
- ABA:
-
abscisic acid
- BAP:
-
6-benzylaminopurine
- IBA:
-
indole-3-butyric acid
- IAA:
-
indole-3-acetic acid
- FAA:
-
formalin + acetic acid + alcohol
- MS:
-
Murashige and Skoog
- PEG:
-
polyethylene glycol
- SEM:
-
scanning electron microscope
- TDZ:
-
thidiazuron
- WPM:
-
Woody plant medium
References
Almeyda, N., Martin, F.W.: Cultivation of neglected tropical fruits with promise 1. The mangosteen (Garcinia mangostana L.). — US Agr. Res. Service South Region 155: 1–18, 1976.
Blazquez, S., Olmos, E., Hernandez, J.E., Fernandez-Garcia, N., Fernandez, J.A., Piqueras, A.: Somatic embryogenesis in saffron (Crocus sativus L.). Histological differentiation and implication of some components of the antioxidant enzymes system. — Plant Cell Tissue Organ Cult. 97: 49–57, 2009.
Corbineau, F., Come, D.: Experiments on the storage of seeds and seedlings of Symphonia globulifera L.f. (Guttiferae). — Seed Sci. Technol. 14: 585–591, 1986.
Do Nascimento, W.M.O., De Carvalho, J.E.U., Muller, C.H.: Morphological characterization of seeds and seedlings to Rheedia acuminata. — Rev. Bras. Frutic. 24: 555–558, 2002.
Dudits, D., Gyorgyey, L., Bako, L.: Molecular biology of somatic embryogenesis. — In: Thorpe, T.A. (ed.): In Vitro Embryogenesis in Plants. Pp. 267–308. Kluwer Academic Publishers, Dordrecht — Boston — London 1995.
Evans, D.E. (ed.): Plant Cell Culture. — Brookes University, Oxford 2003.
Feher, A.: The initiation phase of somatic embryogenesis: what we know and what we don’t. — Acta biol. Szeged 52: 53–56, 2008.
Gairi, A., Rahid, A.: TDZ-induced somatic embryogenesis in non-responsive caryopses of rice using a short treatment with 2.4-D. — Plant Cell Tissue Organ Cult. 76: 29–34, 2003.
Giridhar, P., Indu, E.P., Ravishankar, G.A., Chandrasekar, A.: Influence of TRIA on somatic embryogenesis in Coffea arabica L. and Coffea canephora P. ex FR. — In Vitro cell. dev. Biol. Plant 40: 200–203, 2004.
Goh, H.K.L., Rao, A.N., Loh, C.S.: In vitro plantlet formation in mangosteen (Garcinia mangostana L.). — Ann. Bot. 62: 87–93, 1988.
Ha, C.O., Sands, V.E., Soupadmo, E., Jong, K.: Reproductive patterns of selected understorey trees in the Malaysian rainforest: the apomictic species. — Bot. J. Linn. Soc. 97: 317–331, 1988.
Horn, C.L.: Existence of only one variety of cultivated mangosteen explained by asexually formed ’seed’. — Science 92: 237–238, 1940.
Hussain, S.S., Rao, A.Q., Husnain, T., Riazuddin, S.: Cotton somatic embryo morphology affects its conversion to plant. — Biol. Plant. 53: 307–311, 2009.
Jalil, M., Chee, W.W., Othman, R.Y., Khalid, N.: Morphological examination on somatic embryogenesis of Musa acuminate cv. Mas (AA). — Sci. Hort. 117: 335–340, 2008.
Kiran Ghanti, S., Sujata, K.G., Srinath Rao, M., Kavi Kishor, P.B.: Direct somatic embryogenesis and plant regeneration from immature explants of chickpea. — Biol. Plant. 54: 121–125, 2010.
Lim, A.L.: The embryology of Garcinia mangostana L. (Clusiaceae). — Garden Bull. Singapore 37: 93–103, 1984.
Litz, R.E.: Somatic embryogenesis from cultured leaf explants of the tropical tree Euphoria longan Stend. — J. Plant Physiol. 132: 459–466, 1988.
Llyod, G.B., Mc Cown, B.H.: Commercially feasible micropropagation of mountain laurel, Kalmia latifolia, by use of shoot-tip culture. — Proc. Int. Plant Propag. Soc. 30: 421–427, 1980.
Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassay with tobacco tissue culture. — Physiol. Plant. 15: 473–497, 1962.
Murthy, B.N.S., Murch, S.J., Saxena, P.K.: Thidiazuron induced somatic embryogenesis in intact seedlings of peanut (Arachis hypogaea): endogenous growth regulator levels and significance of cotyledons. — Physiol. Plant. 94: 268–276, 1995.
Nagata, T., Ishida, S., Hasezawa, S., Takahashi, Y.: Genes involved in the dedifferentiation of plant cells. — Int. J. dev. Biol. 38: 321–327, 1994.
Normah, M.N., Nor-Azza, A.B., Aliudin, R.: Factors affecting in vitro shoot proliferation and ex vitro establishment of mangosteen. — Plant Cell Tissue Organ Cult. 43: 291–294, 1995.
Normah, M.N., Rosnah, H., Nor-Azza, A.B.: Multiple shoots and callus formation from seeds of mangosteen (Garcinia mangostana L.) cultured in vitro. — Acta Hort. 292: 87–91, 1992.
Richard, A.J.: Studies in Garcinia, dioecious tropical forest tree: the origin of mangosteen (G. mangostana L.). — Bot. J. Linn. Soc. 103: 301–308, 1990.
Shekhawat, G.S., Mathur, S., Batra, A.: Role of phytohormones and nitrogen in somatic embryogenesis induction in cell culture derived from leaflets of Azadirachta indicia. — Biol. Plant. 53: 707–710, 2009.
Sprecher, M.A.: Etude sur la semance et la germination du Garcinia mangostana L. — Rev. Gen. Bot. 31: 513–531, 1919.
Su, W.W., Hwang, W., Kim, S.Y., Sagawa, Y.: Induction of somatic embryogenesis in Azadirachta indica. — Plant Cell Tissue Organ Cult. 50: 91–95, 1997.
Te-Chato, S., Lim, M.: Plant regeneration of mangosteen via nodular callus formation. — Plant Cell Tissue Organ Cult. 59: 89–93, 1999.
Te-Chato, S., Lim, M.: Improvement of mangosteen micropropagation through meristematic nodular callus formation from in vitro-derived leaf explants. — Scientia Hort. 86: 291–298, 2000.
Te-Chato, S., Lim, M., Suranilpong, P.: Embryogenic callus induction in mangosteen (Garcinia mangostana L.). — Songklanakarin J. Sci. Technol. 1: 115–120, 1995.
Thorpe, T.A., Stasolla, C.: Somatic embryogenesis. — In: Bhojwani, S.S., Soh, W.J. (ed.): Current Trends in the Embryology of Angiosperms. Pp. 279–336. Kluwer Academics Publishers, Dordrecht 2001.
Vestal, P.A.: The significance of comparative anatomy in establishing the relationship of the Hypericaceae to the Guttiferae and their allies. — Philippine J. Sci. 64: 199–256, 1937.
Vila, S., Gonzalez, A., Rey, H., Mroginski, L.: Somatic embryogenesis and plant regeneration in Cedrela fissilis. — Biol. Plant. 53: 383–386, 2009.
Visser, C., Qureshi, J.A., Gill, R., Saxena, P.K.: Morphoregulatory role of thidiazuron. — Plant Physiol. 99: 1704–1797, 1992.
Von Arnold, S., Sabala, I., Bozhkov, P., Dyachock, J., Filonova, L.: Developmental pathways of somatic embryogenesis. — Plant Cell Tissue Organ Cult. 69: 233–240, 2002.
West, M.A.L., Harada, J.J.: Embryogenesis in higher plants: an overview. — Plant Cell 5: 1361–1369, 1993.
Zimmerman, J.L.: Somatic embryogenesis: a model for early development in higher plants. — Plant Cell 5: 1411–1423, 1993.
Acknowledgements
We are grateful to the Malaysian Ministry of Science, Technology and Innovation for funding this project (Grant No. 05-01-02-SF0340). We thank Alena Sanusi for her editorial comments on the paper.
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Elviana, M., Rohani, E.R., Ismanizan, I. et al. Morphological and histological changes during the somatic embryogenesis of mangosteen. Biol Plant 55, 731–736 (2011). https://doi.org/10.1007/s10535-011-0177-5
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DOI: https://doi.org/10.1007/s10535-011-0177-5