Abstract
In vitro cultures of Azadirachta indica A. Juss. were raised by first culturing the root segments on modified Murashige and Skoog (MS) medium supplemented with 8.88 μM 6-benzylaminopurine (BAP), 9.84 μM N6-(2-isopentenyl) adenine (2iP), 5.71 μM indole-3-acetic acid (IAA), 81.43 μM adenine hemisulphate and 2.27 μM putrescine for 2 d followed by their transfer to the same medium except containing one-tenth of the initially used concentrations of BAP, 2iP and IAA. The regenerated shoots sustained proliferation in the basal medium supplemented with 1.11 μM BAP, 1.43 μM IAA and 135.72 μM adenine hemisulphate. The isolated shoots were rooted to produce plantlets in the presence of 2.46 μM indole-3-butyric acid (IBA). The plantlets showed uniform luxuriant growth under field conditions. True-to-type nature of the field-grown root-regenerated plants was ascertained by random amplified polymorphic DNA (RAPD) analysis.
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Abbreviations
- BAP:
-
6-benzylaminopurine
- 2,4-D:
-
2,4-dichlorophenoxyacetic acid
- IAA:
-
indole-3-acetic acid
- IBA:
-
indole-3-butyric acid
- IPA:
-
indole-3-propionic acid
- 2iP:
-
N6-(2-isopentenyl) adenine
- MS medium:
-
Murashige and Skoog medium
- NAA:
-
α-naphthaleneacetic acid
- NOA:
-
α-naphthoxyacetic acid
- PVP:
-
polyvinylpyrollidone
- RAPD:
-
random amplified polymorphic DNA
- TDZ:
-
thidiazuron
- Z:
-
zeatin
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Acknowledgements
The authors thank the Director, National Botanical Research Institute (NBRI), Lucknow for the facilities provided. The first author also thanks the Council of Scientific and Industrial Research (CSIR), India, for awarding her Senior Research Fellowship. Thanks are also due to Mr. R.S. Tripathi and Mr. O.P. Sharma for technical assistance.
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Arora, K., Sharma, M., Srivastava, J. et al. In vitro cloning of Azadirachta indica from root explants. Biol Plant 55, 164–168 (2011). https://doi.org/10.1007/s10535-011-0023-9
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DOI: https://doi.org/10.1007/s10535-011-0023-9