Abstract
The full-length cDNA of LeSGR1 was cloned from tomato by RT-PCR and RACE. The cDNA encoded a protein of 272 amino acid residues and was deposited in GenBank (accession No. DQ100158). Northern analysis suggests that LeSGR1 gene specifically expresses in senescent leaves and mature fruits of tomatoes. Desiccation and flooding induce the expression of LeSGR1 in tomato leaves and stems. Both in ethylene-insensitive mutants (Nr) and ripening inhibitor mutants (rin), the expression of LeSGR1 is markedly decreased compared with that in the wild type. Alignment of the nucleotide sequence of SGR1 cloned from the tomato green flesh (gf) mutant with that from the wild type tomato shows a single nucleotide change leading to an amino acid substitution in gf mutant. Furthermore, LeSGR1 gene silencing by RNA interference results in inhibited chlorophyll degradation similar to the phenotype in gf mutant. Thus, we conclude that LeSGR1 is crucial to chlorophyll degradation and the mutation of SGR1 protein might be responsible for gf tomato properties.
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Abbreviations
- AC++ :
-
Ailsa Craig
- B:
-
breaker
- Chl:
-
chlorophyll
- dpa:
-
days post anthesis
- gf :
-
green flesh
- IMG:
-
immature green
- MG:
-
mature green
- ML:
-
mature leaf
- Nr :
-
never ripe
- ORF:
-
open reading frame
- RACE:
-
rapid amplification of cDNA ends
- rin :
-
ripening inhibitor
- RT PCR:
-
reverse transcriptase polymerase chain reaction
- SL:
-
senescent leaf
- UTR:
-
untranslated region
- YL:
-
young leaf
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Acknowledgments
This work was supported by the National Natural Science Foundation of China (No.30771464 and No.30871709.) and by China Postdoctoral Science Foundation (No.20060390677 and No.20070420717). We thank Prof. J.R. Botella (University of Queensland) for critical reading of the manuscript.
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Hu, Z.L., Deng, L., Yan, B. et al. Silencing of the LeSGR1 gene in tomato inhibits chlorophyll degradation and exhibits a stay-green phenotype. Biol Plant 55, 27–34 (2011). https://doi.org/10.1007/s10535-011-0004-z
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DOI: https://doi.org/10.1007/s10535-011-0004-z