Abstract
The aim of this study was to investigate acclimation of micropropagated plants of Rhododendron ponticum subsp. baeticum to different irradiances and recovery after exposure to high irradiance. Plants grown under high (HL) or intermediate (IL) irradiances displayed higher values of maximum electron transport rate (ETRmax) and light saturation coefficient (Ek) than plants grown under low irradiance (LL). The capacity of tolerance to photoinhibition (as assessed by the response of photochemical quenching, qp) varied as follows: HL > IL > LL. Thermal energy dissipation (qN) was also affected by growth irradiance, with higher saturating values being observed in HL plants. Light-response curves suggested a gradual replacement of qp by qN with increasing irradiance. Following exposure to irradiance higher than 1500 μmol m−2 s−1, a prolonged reduction of the maximal photochemical efficiency of PS 2 (Fv/Fm) was observed in LL plants, indicating the occurrence of chronic photoinhibition. In contrary, the decrease in Fv/Fm was quickly reverted in HL plants, pointing to a reversible photoinhibition.
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Abbreviations
- Ek :
-
light saturation coefficient
- ETR:
-
electron transport rate
- Fm :
-
maximum fluorescence
- F0 :
-
initial fluorescence
- Fv :
-
variable fluorescence
- HL:
-
high irradiance
- IL:
-
intermediate irradiance
- LL:
-
low irradiance
- PS 1:
-
photosystem 1
- PS 2:
-
photosystem 2
- qp :
-
photochemical quenching
- qN :
-
non-photochemical quenching
- α:
-
initial linear slope
- ΦPS2 :
-
effective quantum efficiency of PS 2
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Acknowledgements
This work was supported by INIAP — project AGRO 301 and Portuguese Foundation for Science and Technology (FCT) — post-doctoral grant (SFRH/BPD/35410/2007).
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Osório, M.L., Osório, J. & Romano, A. Chlorophyll fluorescence in micropropagated Rhododendron ponticum subsp. baeticum plants in response to different irradiances. Biol Plant 54, 415–422 (2010). https://doi.org/10.1007/s10535-010-0076-1
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DOI: https://doi.org/10.1007/s10535-010-0076-1