Abstract
Seven-day-old maize seedlings grown in a nitrogen-free hydroponic culture were exposed for 48 h to 0, 100 and 300 μM trans-cinnamic, p-coumaric, ferulic, caffeic acids, umbelliferone and 200 μM KNO3. Net nitrate uptake was affected by trans-cinnamic, ferulic and p-coumaric acids in a concentration-dependent manner, and trans-cinnamic acid appeared to be the strongest inhibitor. Conversely, at low concentrations, caffeic acid stimulated net nitrate uptake while umbelliferone did not influence it. After 24 h of treatment, plasma membrane H+-ATPase activity significantly decreased in a concentration-dependent manner in response to trans-cinnamic, ferulic and p-coumaric acids, while umbelliferone and caffeic acid had no effect on H+-ATPase activity.
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Abbreviations
- BPT:
-
1,3-bis[tris (hydroxymethyl)-methylamino]-propane
- DDT:
-
1,4-dithioerythritol
- EDTA:
-
ethylenediaminetetraacetic acid
- EGTA:
-
ethyleneglycoltetraacetic acid)
- IC50 :
-
the dose causing 50 % inhibition of the plasma membrane H+-ATPase activity
- MES:
-
2-[N-morpholino]ethanesulphonic acid
- NNUR:
-
net nitrate uptake rate
- PMSF:
-
phenylmethylsulphonyl fluoride
References
Abenavoli, M.R., De Santis, C., Sidari, M., Sorgonà, A., Badiani, M., Cacco, G.: Influence of coumarin on the net nitrate uptake in durum wheat (Triticum durum Desf. cv. Simeto). — New Phytol. 150: 619–627, 2001.
Abenavoli, M.R., Sorgonà, A., Albano, S., Cacco, G.: Coumarin differentially affects the morphology of different root types of maize seedlings. — J. chem. Ecol. 30: 1871–1883, 2004.
Aslam, M., Travis, R.L., Huffaker, R.C.: Comparative kinetics and reciprocal inhibition of nitrate and nitrite uptake in roots of uninduced and induced barley (Hordeum vulgare L.) seedlings. — Plant Physiol. 99: 1124–1133, 1992.
Bergmark, C.L., Jackson, W.A., Volk, R.J., Blum, U.: Differential inhibition by ferulic acid of nitrate and ammonium uptake on Zea mays L. — Plant Physiol. 98: 639–645, 1992.
Booker, F.L., Blum, U., Fiscus, E.L.: Short-term effects of ferulic acid on ion uptake and water relations in cucumber seedlings. — J. exp. Bot. 43: 649–655, 1992.
Bradford, M.M.: A rapid and sensitive method of quantitation of microgram quantities of protein utilizing the principle of protein dye binding. — Anal. Biochem. 72: 248–254, 1976.
Crawford, N.M.: Nitrate: nutrient and signal for plant growth. — Plant Cell 7: 859–868, 1995.
Forbush, B.: Assay of the Na+K+-ATPase in plasma membrane preparations: increasing the permeability of membrane vesicles using sodium dodecyl sulfate buffered with bovine serum albumin. — Anal. Biochem. 128: 159–163, 1983.
Forde, B.G.: Nitrate transporters in plants: structure, function and regulation. — Biochem. biophys. Acta 1465: 219–235, 2000.
Fujita, K.I., Kubo, I.: Synergism of polygodial and transcinnamic acid on inhibition of root elongation in lettuce seedling growth bioassays. — J. chem. Ecol. 29: 2253–2263, 2003.
Giannini, J.L., Ruiz-Cristin, J., Briskin, D.P.: A small scale procedure for the isolation of transport competent vesicles from plants tissues. — Anal. Biochem. 174: 561–567, 1988.
Glass, A.D.M.: Influence of phenolic acids upon ion uptake. III. Inhibition of potassium absorption. J. exp. Bot. 25: 1104–1113, 1974.
Goldsmith, J., Livoni, J.P., Norberg, C.L., Segel, I.H.: Regulation of nitrate reductase in Penicillium chrysogenum by ammonium ion. — Plant Physiol. 52: 362–367, 1973.
Hejl, A.M. Koster, K.L.: The allelochemical sorgoleone inhibits root H+-ATPase and water uptake. — J. chem. Ecol. 11: 2181–2191, 2004.
Hoagland, D.R. Arnon, D.I.: The water-culture method for plants without soil. — Calif. Agr. Sta. Circ. 347: 32, 1950.
Jensén, P., Biegus, B., Caspersen, S., Adalsteinsson, S., Sundin, P.: Influence of ferulic acid, P, K and Ca on growth and mineral nutrition of lettuce (Lactuca sativa L.) in soilless culture. — Acta Hort. 361: 311–316, 1994.
Kupidlovska, E., Kowalec, M., Sulkowski, G., Zobel, A.M.: The effect of coumarins on root elongation and ultrastructure of meristematic cell protoplast. — Ann. Bot. 73: 525–530, 1994.
Li, W.G., Wang, J.L., Shen, J.J., Wang, J.B.: Nitrate reduction in ramets of a clonal plant Eichhornia crassipes responding to nitrate availability during clonal growth stage. — Biol. Plant. 53: 171–174, 2009.
Palmgren, M.G.: Plant plasma membrane H+-ATPases: powerhouses for nutrient uptake. — Annu. Rev. Plant Physiol. Plant mol. Biol. 52: 817–845, 2001.
Pospíšil, F., Hrubcová M., Cvikrová M.: Anodic oxidation of coumarie and caffeic acids and their effects on nitrate uptake and nitrate reductase in Nicotiana tabacum cell suspension. — Biol. Plant. 29: 175–182, 1987.
Reigosa, M.J., Pazos-Malvido, E.: Phytotoxic effects of 21 plant secondary metabolites on Arabidopsis thaliana germination and root growth. — J. chem. Ecol. 33: 1456–1466, 2007.
Reynolds, T.: Comparative effects of heterocyclic compounds on inhibition of lettuce fruit germination. — J. exp. Bot. 40: 391–404, 1989.
Rubio, G, Sorgonà, A., Lynch, J.P.: Spatial mapping influx in bean root systems using digital autoradiography. — J. exp. Bot. 55: 2269–2280, 2004.
Santi, S., Locci, G., Pinton, R., Cesco, S., Varanini, Z.: Plasma membrane H+-ATPase in maize roots induced for NO3 − uptake. — Plant Physiol. 109: 1277–1283, 1995.
Serrano, P.: Structure and function of plasma membrane ATPase. — Annu. Rev. Plant. Physiol. Plant mol. Biol. 40: 61–94, 1989.
Sze, H.: H+-translocating ATPases: advances using membrane vesicles. — Annu Rev Plant Physiol. 36: 113–122, 1985.
Wong, W.S., Guo, D., Wong, X.L., Yin, Z.Q., Xia, B., Li, N.: Study of cis-cinnamic acid in Arabidopsis thaliana. — Plant. Physiol Biochem. 43: 929–937, 2005.
Yu, J.Q., Matsui, Y.: Effects of root exudates of cucumber (Cucumis sativus) and allelochemicals on ion uptake by cucumber seedlings. — J. chem. Ecol. 23: 817–827, 1997.
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Abenavoli, M.R., Lupini, A., Oliva, S. et al. Allelochemical effects on net nitrate uptake and plasma membrane H+-ATPase activity in maize seedlings. Biol Plant 54, 149–153 (2010). https://doi.org/10.1007/s10535-010-0024-0
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DOI: https://doi.org/10.1007/s10535-010-0024-0