Abstract
A protocol for in vitro multiplication of Capparis decidua (Forsk.) Edgew. has been developed from cultured leaves procured from multiplying axillary shoots on the cultured nodal explants. The highest efficiency of shoot formation was observed on Murashige and Skoog (MS) medium containing 2 mg dm−3 benzyladenine (BA) and 0.5 mg dm−3 1-naphthaleneacetic acid. The regenerated shoots were transferred to MS medium containing 3 mg dm−3 BA for growth and proliferation. Shoots above 2 cm in length were transferred to MS medium supplemented with 1 mg dm-3 indole-3-butyric acid plus 0.5 mg dm−3 indole-3-acetic acid for root induction. No variation was detected among the micropropagated plants by randomly amplified polymorphic DNA (RAPD) markers.
Article PDF
Avoid common mistakes on your manuscript.
Abbreviations
- BA:
-
benzyladenine
- IAA:
-
indole-3-acetic acid
- IBA:
-
indole-3-butyric acid
- MS:
-
Murashige and Skoog
- NAA:
-
1-naphthaleneacetic acid
- RAPD:
-
randomly amplified polymorphic DNA
References
Ashmore, S.E.: Status Report on the Development and Application of In Vitro Techniques for the Conservation and Use of Plant Genetic Resources. — IPGRI, Rome 1997.
Carvalho, L., Goulão, L., Oliveira, C., Gonçalves, J.C., Amâncio, S.: RAPD assessment for identification of clonal identity and genetic stability of in vitro propagated chestnut hybrids. — Plant Cell Tissue Organ Cult. 77: 23–27, 2004.
Doyle, J.J, Doyle, J.L.: Isolation of plant DNA from fresh tissue. — Focus 12: 13–15, 1990.
Espinosa, A.C., Pijut, P.M., Michler, C.H.: Adventitious shoot regeneration and rooting of Prunus serotina in in vitro cultures. — HortScience 41: 193–201, 2006.
Feyissa, T., Welander, M., Negash, L.: Genetic stability, ex vitro rooting and gene expression studies in Hagenia abyssinica, — Biol. Plant. 51: 15–21, 2007.
Gagliardi, R.F., Hanai, L.R., Pacheco, G., Oliveira, C.A., Carneiro, L.A., Valls, J.F.M., Mansur, E., Vieira, M.L.C.: Assessment of genetic stability among in vitro plants of Arachis retusa using RAPD and AFLP markers for germplasm preservation, — J. Integr. Plant Biol, 49: 307–312, 2007.
Gyves, E.M.D., Sparks, C.A., Fieldsend, A.F., Lazzeri, P.A., Jones H.D.: High frequency of adventitious shoot regeneration from commercial cultivars of evening primrose (Oenothera spp.) using thidiazuron. — Ann. appl. Biol. 138: 329–332, 2001.
Joshi, A., Kothari, S.L.: High copper levels in the medium improve shoot bud differentiation and elongation from the cultured cotyledons of Capsicum annuum L. — Plant Cell Tissue Organ Cult. 88: 127–133, 2007.
Joshi, P., Dhawan, V.: Assessment of genetic fidelity of micropropagated Swertia chirayita plantlets by ISSR marker assay. — Biol. Plant. 51: 22–26, 2007.
Khan, T.I., Frost, S.: Floral biodiversity: a question of survival in the Indian Thar desert. — Environmentalist 21: 231–236, 2001.
Khan, T.I., Dular, A.K., Solomon, D.M.: Biodiversity conservation in the Thar desert with emphasis on endemic and medicinal plants. — Environmentalist 23: 137–144, 2003.
Mohammadi, S.N., Prasanna, B.M.: Analysis of genetic diversity in crop plants—salient statistical tools and considerations. — Crop Sci. 43: 1235–1248, 2003.
Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassays with tobacco tissue cultures. — Physiol. Plant. 15: 473–497, 1962.
Nanda, R.M., Das, P., Rout, G.R.: In vitro clonal propagation of Acacia mangium Willd. and its evaluation of genetic stability through RAPD marker. — Ann. Forest Sci. 61: 381–386, 2004.
Piola, F., Rohr, R., Heizmann, P.: Rapid detection of genetic variation within and among in vitro propagated cedar (Cedrus libani Loudon) clones. — Plant Sci. 141: 159–163, 1999.
Rady, M.R.: In vitro culture of Gypsophila paniculata L. and random amplified polymorphic DNA analysis of the propagated plants. — Biol. Plant. 50: 507–513, 2006.
Ryynanen, L., Aronen, T.: Genome fidelity during short- and long term tissue culture and differentially cryostored meristems of silver birch (Betula pendula). — Plant Cell Tissue Organ Cult. 83: 21–32, 2005.
Sanatombi, K., Sharma, G.J.: In vitro plant regeneration in six cultivars of Capsicum spp. using different explants. — Biol. Plant. 52: 141–145, 2008.
Sharma, P.K., Tyagi, P., Sharma, K.C., Kothari, S.L.: Clonal micropropagation of Crataeva adansonii (DC.) Prodr.: a multipurpose tree. — In vitro cell. dev. Biol. Plant. 39: 156–160, 2003.
Singh, A., Negi, M.S., Moses, V.K., Venkateswarlu, B., Srivastava, P.S., Lakshmikumaran, M.: Molecular analysis of micropropagated neem plants using AFLP markers for ascertaining clonal fidelity. — In vitro cell. dev. Biol. Plant 38: 519–524, 2002.
Soneji, J.R., Rao, P.S., Mhatre, M.: Suitability of RAPD for analyzing spined and spineless variant regenerants of pineapple (Ananas comosus L. Merr.). — Plant mol. Biol. Rep. 20: 307a–307i, 2002.
Soniya, E.V., Banerjee, N.S., Das, M.R.: Genetic analysis of somaclonal variation among callus-derived plants of tomato. — Curr. Sci. 80: 1213–1215, 2001.
Tyagi, P., Khanduja, S., Kothari, S.L.: Somatic embryogenesis in Capparis deciduas (Forsk) Edgew. — a multipurpose agroforestry plant. — J. Plant Biochem. Biotechnol. 14: 197–200, 2005.
Tyagi, P., Kothari, S.L.: Micropropagation of Capparis decidua through in vitro shoot proliferation on nodal explants of mature tree and seedling explants. — J. Plant Biol. Biotechnol. 6: 19–23, 1997.
Tyagi, P., Kothari, S.L. Continuous shoot production for micropropagation of Capparis decidua — a tree of arid agroforestry system. — J. indian bot. Soc. 80: 5–8, 2001.
Virscek-Marn, M., Bohanec, B., Javornik, B.: Adventitious shoot regeneration from apple leaves — optimization of the protocol and assessment of genetic variation among regenerants. — Phyton 39: 61–70, 1999.
Welsh, J., McClelland, M.: Fingerprinting genomes using PCR with arbitrary primers. — Nucl. Acids Res. 18: 7213–7219, 1990.
Acknowledgements
Purnima Tyagi thanks the Department of Science and Technology (DST WOS-A No: SR/WOS-A/LS-236/2004), New Delhi for the financial support.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Tyagi, P., Khanduja, S. & Kothari, S. In vitro culture of Capparis decidua and assessment of clonal fidelity of the regenerated plants. Biol Plant 54, 126–130 (2010). https://doi.org/10.1007/s10535-010-0019-x
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10535-010-0019-x