Abstract
A highly efficient protocol for plant regeneration from cotyledonary node of two chickpea (Cicer arietinum L.) cultivars ICCV-10 and Annigeri used phenylacetic acid (PAA). The Murashige and Skoog (MS) medium supplemented with 2.0 mg dm−3 6-benzylaminopurine (BAP) and 1.0 mg dm−3 PAA was used for induction of bud formation. Buds were elongated on MS medium supplemented either with only 0.75 mg dm−3 gibberellic acid (GA3) or 0.2 mg dm−3 GA3 + 0.6 mg dm−3 PAA. The elongated shoots were then transferred onto rooting medium containing 1 mg dm−3 PAA. The frequency of multiple shoot induction and rooting was higher in Annigeri as compared to ICCV-10. The complete plantlets with well-developed roots were transferred to pots containing sterilized soil and sand in the ratio 3:1 where they survived (74 %) and set normal seeds.
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Abbreviations
- BAP:
-
N6-benzylaminopurine
- GA3 :
-
gibberellic acid
- KIN:
-
kinetin
- MS:
-
Murashige and Skoog
- IAA:
-
indole-3-acetic acid
- IBA:
-
indole-3-butyric acid
- NAA:
-
α-naphthaleneacetic acid
- PAA:
-
phenylacetic acid
- TDZ:
-
thidiazuron
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Kiran Ghanti, S., Sujata, K.G. & Srinath Rao, M. The effect of phenyl acetic acid on shoot bud induction, elongation and rooting of chickpea. Biol Plant 53, 779–783 (2009). https://doi.org/10.1007/s10535-009-0143-7
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DOI: https://doi.org/10.1007/s10535-009-0143-7