Abstract
Proline accumulation was studied in the leaves of Glycine max (L.) Merr. subjected to salt stress in the presence of aminoguanidine (AG, a specific inhibitor of diamine oxidase, DAO) and exogenous putrescine (Put). Both DAO activity and proline content were increased while endogenous Put content was decreased in soybean leaves under 50 to 150 mM NaCl. There was a negative correlation between proline accumulation and endogenous Put content. The addition of AG during NaCl stress inhibited DAO activity, caused Put accumulation and a 15 to 20 % decrease in proline content. Application of 1 mM Put to NaCl solution markedly increased proline content. The promotive effect of Put application could be alleviated by the treatment with Put plus AG. Moreover an application of AG had no effect on proline accumulation in soybean seedlings grown under normal condition. These results indicate that the quantitative contribution of Put degradation to proline formation is 15 to 20 %.
Article PDF
Avoid common mistakes on your manuscript.
Abbreviations
- AG:
-
aminoguanidine
- DAO:
-
diamine oxidase
- PAs:
-
polyamines
- PAO:
-
polyamine oxidase
- Put:
-
putrescine
- Spd:
-
spermidine
- Spm:
-
spermine
References
Ashraf, M., Harris, P.J.C.: Potential biochemical indicators of salinity tolerance in plants.-Plant Sci. 166: 3–16, 2004.
Aziz, A., Martin-Tanguy, J., Larher, F.: Stress-induced changes in polyamine and tyramine levels can regulate proline accumulation in tomato leaf discs treated with sodium chloride.-Physiol. Plant. 104: 195–202, 1998.
Bouchereau, A., Aziz, A., Larher, F., Martin-Tanguy, J.: Polyamines and environmental challenges: recent development.-Plant Sci. 140: 103–125, 1999.
Flores, H.E., Galston, A.W.: Analysis of polyamines in higher plants by high performance liquid chromatography.-Plant Physiol. 69: 701–706, 1982.
Gaspar, T., Franck, T., Bibis, B., Jouve, L., Hausman, J.F., Dommes, J.: Concepts in plant stress physiology. Application to plant tissue cultures.-Plant Growth Regul. 37: 363–385, 2002.
Gaspar, T., Kevers, C., Hausman, J.F., Faivre-Rampant, O., Boyer, N., Domme, J.S., Penel, C., Greppin, H.: Integrating phytohormone metabolism and action with primary biochemical pathways: inter-relationship between auxins, cytokinins, ethylene and polyamines in growth and development processes.-In: Greppin, H., Penel, C., Broughton, W.J., Strasser, R. (ed.): Integrated Plant Systems. Pp. 163–191. University of Geneva, Geneva 2000.
Hien, D.T., Jacobs, M., Angenon, G., Hermans, C., Thu, T.T., Son, L.V., Roosens, N.H.: Proline accumulation and Δ1-pyrroline-5-carboxylate synthetase gene properties in three rice cultivars differing in salinity and drought tolerance.-Plant Sci. 165: 1059–1068, 2003.
Lefèvre, I., Gratia, A., Lutts, S.: Discrimination between the ionic and osmotic component of stress in relation to free polyamine level in rice.-Plant Sci. 161: 943–952, 2001.
Parida, A.K., Das, A.B.: Salt tolerance and salinity effects on plants: a review.-Ecotoxicol. Environ. Safety 60: 324–349, 2005.
Rea, G., Laurenzi, M., Tranquilli, E., D’Ovidio, R., Federico, R., Angelini, R.: Developmentally and wound-regulated expression of the gene encoding a cell wall copper amine oxidase in chickpea seedlings.-FEBS Lett. 437: 177–182, 1998.
Sanchez, D.H., Cuevas, J.C., Chiesa, M.A., Ruiz, O.A.: Free spermidine and spermine content in Lotus glaber under long-term salt stress.-Plant Sci. 168: 541–546, 2005.
Santa-Cruz, A., Acosta, M., Rus, A., Bolarin, M.C.: Short-term salt tolerance mechanisms in differentially salt tolerant tomato species.-Plant Physiol. Biochem. 37: 65–71, 1999.
Smith, T.A.: The di-and polyamine oxidases of higher plants.-Biochem. Trans. 13: 319–322, 1985.
Sotiropoulos, T.E.: Effect of NaCl and CaCl2 on growth and contents of minerals, chlorophyll, proline and sugars in the apple rootstock M 4 cultured in vitro.-Biol. Plant. 51: 177–180, 2007.
Su, G.X., An, Z.F., Zhang, W.H., Liu, Y.L.: Light promotes the synthesis of lignin through the production of H2O2 mediated by diamine oxidases in soybean hypocotyls.-J. Plant Physiol. 162: 1297–1303, 2005.
Su, G.X., Yu, B.J., Zhang, W.H., Liu, Y.L.: Higher accumulation of γ-aminobutyric acid induced by salt stress through stimulating the activity of diamine oxidases in soybean roots.-Plant Physiol. Biochem. 45: 560–566, 2007.
Tonon, G., Kevers, C., Faivre-Rampant, O., Graziani, M., Gaspar, T.: Effect of NaCl and mannitol iso-osmotic stresses on proline and free polyamine levels in embryogenic Fraxinus angustifolia callus.-J. Plant Physiol. 161: 701–708, 2004.
Tripathi, S.B., Gurumurthi, K., Panigrahi, A.K., Shaw, B.P.: Salinity induced changes in proline and betaine contents and synthesis in two aquatic macrophytes differing in salt tolerance.-Biol. Plant. 51: 110–115, 2007.
Troll, W., Lindsley, J.A.: Photometric method for the determination of proline.-J. biol. Chem. 215: 655–660, 1955.
Urano, K., Yosiba, Y., Nanjo, T., Igarashi, Y., Seki, M., Sekiguchi, F., Yamaguchi-Shinozaki, K., Shinozaki, K.: Characterization of Arabidopsis genes involved in biosynthesis of polyamines in abiotic stress responses and developmental stages.-Plant Cell Environ. 26: 1917–1926, 2003.
Zhao, F.G., Sun, C., Liu, Y.L.: Ornithine pathway in biosynthesis activated by salt stress in barley seedlings.-Acta bot. sin. 43: 36–40, 2001.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Su, G.X., Bai, X. Contribution of putrescine degradation to proline accumulation in soybean leaves under salinity. Biol Plant 52, 796–799 (2008). https://doi.org/10.1007/s10535-008-0156-7
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10535-008-0156-7