Abstract
Seed germination and micropropagation protocols of the medicinal species Maytenus canariensis (Loes.) G. Kunkel & Sunding were optimized. In vitro seed germination occurred (86 to 94.7 %) only after treatment of the seeds with H2SO4, followed by surface sterilization and culture on solid nutrient medium without any growth regulators. Micropropagation failed when explants were taken from mature trees, and browning of the nutrient medium frequently occurred despite testing many growth media. Nonetheless, adventitious shoot regeneration was achieved employing axillary or apical buds taken from 2–2.5 months old plantlets obtained after in vitro germination of seeds, following culture on nutrient media supplemented with benzylaminopurine, kinetin and naphthalenacetic acid (NAA), attaining up to 3.9 shoots per explant, after 4–6 months. Root induction was best on a medium containing 4.0 mg dm−3 NAA, achieving a 100 % induction. After hardening of rooted plants, survival after transfer to soil was 71.43 %.
Article PDF
Avoid common mistakes on your manuscript.
Abbreviations
- BAP:
-
benzylaminopurine
- B5 :
-
Gamborg’s medium
- Kin:
-
kinetin
- MS:
-
Murashige and Skoog’s medium
- NAA:
-
naphthaleneacetic acid
- 2,4-D:
-
2,4-dichlorophenoxyacetic acid
References
Aliero, B.L.: Effects of sulphuric acid, mechanical scarification and wet heat treatments on germination of seeds of African locust bean tree, Parkia biglobosa.-African J. Biotechnol. 3: 179–181, 2004.
Buffa-Filho, W., Da Silva-Bolzani, V., Furlan, M., Vaz-Pereira, S.I., Soares-Pereira, A.M., Castro-Franca, S.: In vitro propagation of Maytenus ilicifolia (Celastraceae) as potential source for antitumoral and antioxidant quinomethide triterpenes production. A rapid quantitative method for their analysis by reverse-phase high-performance liquid chromatography.-Arkivoc 6: 137–146, 2004.
Gamborg, O.L., Miller, R.A., Ojima, K.: Nutrient requirements of suspension cultures of soybean root cells.-Exp. Cell Res. 50: 151–158, 1968.
Li, J., Zhao, P., Lu, C., Liu, X., Zeng, Y., Shen, Y.: Studies on lipoxygenase and polyhydroxy fatty acid in SA-elicited Maytenus hookerii suspension cells.-Yunnan Zhiwu Yanjiu 26: 543–548, 2004.
Lu, C., Zhang, J., Gan, F., Shen, Y.: Chemical constituents of suspension cell cultures of Maytenus hookeri.-Acta bot. sin. 44: 603–610, 2002.
Matu, E.N., Lindsey K.L., Van Staden, J.: Micropropagation of Maytenus senegalensis (Lam.) Excell.-S. Afr. J. Bot. 72: 409–415, 2006.
Murashige, T., Skoog, F.: A revised medium for rapid growth and bio-assays with tobacco tissue cultures.-Physiol. Plant. 15: 473–497, 1962.
Nair, L., Seeni, S.: Rapid in vitro multiplication and restoration of Celastrus paniculatus Willd. subsp. paniculatus (Celastraceae), a medicinal woody climber.-Indian J. exp. Bot. 39: 697–704, 2001.
Rao, M.S., Purohit, S.D.: In vitro shoot bud differentiation and plantlet regeneration in Celastrus paniculatus Willd.-Biol. Plant. 50: 501–506, 2006.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Gutiérrez-Nicolás, F., Ravelo, Á.G. & Zárate, R. Seed germination and in vitro propagation of Maytenus canariensis through regeneration of adventitious shoots from axillary and apical buds. Biol Plant 52, 173–176 (2008). https://doi.org/10.1007/s10535-008-0038-z
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/s10535-008-0038-z