Abstract
In this work the volatiles emitted from in vitro shoot-cultures and micropropagated plants of Lavandula viridis L’Hér. were characterized and compared with those obtained from the field-grown mother-plant, using headspace solid phase micro-extraction following by capillary gas chromatography coupled to mass spectrometry (HS-SPME-GC/MS). The headspace composition consisted mainly in oxygenated monoterpenes (66.7–79.2 %), where the major constituents emitted by the mature field-grown mother-plant, in vitro shoot-cultures and micropropagated plants were 1,8-cineole (74.0, 51.9 and 57.8 %) and camphor (2.9, 15.3 and 8.7 %), respectively. The headspace of in vitro shoot-cultures and micropropagated plants showed greater amount of α-pinene, camphene, β-pinene, β-selinene and selina-3,7(11)-diene, when compared with the field-grown mother-plant.
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Abbreviations
- BA:
-
benzyladenine
- GC/MS:
-
gas chromatography coupled to mass spectrometry
- FP:
-
field-grown mother plant
- InV:
-
in vitro shoot cultures
- MP:
-
micropropagated plants
- MS:
-
Murashige and Skoog medium
- SPME:
-
solid phase microextraction
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Gonçalves, S., Serra, H., Nogueira, J.M.F. et al. Headspace-SPME of in vitro shoot-cultures and micropropagated plants of Lavandula viridis . Biol Plant 52, 133–136 (2008). https://doi.org/10.1007/s10535-008-0027-2
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DOI: https://doi.org/10.1007/s10535-008-0027-2