Abstract
Changes in growth and structural properties of Citrus cell line Carvalhal acclimated to 100 mM NaCl in the medium were compared to unacclimated control cells and cells exposed to 100 mM NaCl. Transmission electron microscopy (TEM) showed presence of ring-shaped mitochondria, increase in the number of amyloplasts and lipid bodies, higher cell wall thickness and partitioned vacuoles in acclimated cells.
Article PDF
Avoid common mistakes on your manuscript.
Abbreviations
- TEM:
-
transmission electron microscopy
References
Binzel, M.L., Hess, F.D., Bressan, R.A., Hasegawa, P.M.: Intracellular compartmentation of ions in salt adapted tobacco cells.-Plant Physiol. 86: 607–614, 1988.
Bressan, R.A., Nelson, D.E., Iraki, N.M., LaRosa, P.C.: Reduced cell expansion and changes in cell walls of plant cells adapted to NaCl.-In: Katterman, F. (ed.): Environmental Injury to Plants. Pp. 137–171. Academic Press, San Diego 1990.
Chang, P.F.L., Damsz, B., Kononowicz, A.K., Reuveni, M., Chen, Z., Xu, Y., Hedges, K., Tseng, C.C., Singh, N.K., Binzel, M.L., Narasimhan, M.L., Hasegawa, P.M., Bressan R.A.: Alterations in cell membrane structure and expression of a membrane-associated protein after adaptation to osmotic stress.-Physiol. Plant. 98: 505–516, 1996.
Chinnusamy, V., Jagendorf, A., Zhu, J.K.: Understanding and improving salt tolerance in plants.-Crop Sci. 45: 437–448, 2005.
Ferreira, A.L., Lima-Costa, M.E.: Metabolic responses to salt stress in cell suspension cultures of sensitive and resistant Citrus.-J.H.S.B. 81: 983–988, 2006.
Gosset, D.R., Banks, S.W., Millhollon, E.P., Lucas, M.C.: Antioxidant response to NaCl stress in a control and a NaCl-tolerant cotton cell line grown in the presence of paraquat, buthionine sulfoximine, and exogenous glutathione.-Plant Physiol. 112: 803–809, 1996.
Hasegawa, P.M., Bressan, R.A., Zhu, J.K., Bohnert, I.U.: Plant cellular and molecular responses to high salinity.-Annu. Rev. Plant Physiol. Plant mol. Biol. 51: 463–499, 2000.
Huang, W.L., Liu, F.L.: Carbohydrate metabolism in rice during callus induction and shoot regeneration induced by osmotic stress.-Bot. Bull. Acad. sin. 43: 107–113, 2002.
Kerkeb, L., Donaire, J.P., Rodriguez-Rosales, M.P.: Plasma membrane H+-ATPase activity is involved in adaptation of tomato calli to NaCl.-Physiol. Plant. 111: 483–490, 2001.
Kuiper, P.J.C.: Functioning of plant cell membranes under saline conditions: membrane lipid composition and ATPases. In: Staples, R.C., Toenniessen, G.H. (ed.): Salinity Tolerance in Plants: Strategies for Crop Improvement. Pp. 77–91. Wiley, New York 1984.
Mansour, M.M.M., Salama, K.H.A.: Cellular basis of salinity tolerance in plants.-Environ. exp. Bot. 52: 113–122, 2004.
Mandhania, S., Madan, S. Sawhney, V.: Antioxidant defense mechanism under salt stress in wheat seedlings.-Biol. Plant. 50: 227–231, 2006.
Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassay with tobacco tissue cultures.-Physiol. Plant. 12: 473–497, 1962.
Neto, A.D.A., Prisco, J.T., Enéas-Filho, J., Medeiros, J.V.R., Gomes-Filho, E.: Hydrogen peroxide pre-treatment induces salt-stress acclimation in maize plants.-J. Plant Physiol. 162: 1114–1122, 2005.
Passos, V.M., Santana, N.O., Gama, F.C., Oliveira, J.G., Azevedo, R.A.: Growth and ion uptake in Annona muricata and A. squamosa subjected to salt stress.-Biol. Plant. 49: 285–288, 2005.
Piqueras, A., Olmos, E., Hellín, E.: Cytological changes related with salt tolerance in embryogenic callus of Citrus limon.-Plant Cell Tissue Organ Cult. 39: 13–18, 1994.
Singh, N.K., Bressan, R.A., Carpita, N.C.: Cell walls of tobacco cells and changes in composition associated with reduced growth upon adaptation to water and saline stress.-Plant Physiol. 91: 48–53, 1989.
Yang, J.Y., Yen, H.E.: Early salt stress effects on the changes in chemical composition in leaves of ice plant and Arabidopsis. A Fourier transform infrared spectroscopy study.-Plant Physiol. 130: 1032–1042, 2002.
Yeo, A.: Molecular biology of salt tolerance in the context of whole-plant physiology.-J. exp. Bot. 49: 915–929, 1998.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Ferreira, A.L., Lima-Costa, M.E. Growth and ultrastructural characteristics of Citrus cells grown in medium containing NaCl. Biol Plant 52, 129–132 (2008). https://doi.org/10.1007/s10535-008-0026-3
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/s10535-008-0026-3