Abstract
Multiple shoots were induced by culturing nodal explants excised from 1-month-old aseptic seedlings of red pepper (Capsicum annuum L. cv. Pusa Jwala) on Murashige and Skoog (MS) medium supplemented with (0.1–10 μM) thidiazuron (TDZ). The rate of multiple shoot induction per explant was maximum (14.4 ± 0.06) on MS medium supplemented with 1.0 μM TDZ. Regenerated shoots were elongated well on growth regulator free MS medium. Adventitious roots were induced two weeks after transfer of elongated shoots to MS medium supplemented with auxins (IAA, IBA or NAA) in different concentrations. Optimum root formation frequency was obtained in medium containing 1.0 μM IBA. Ex-vitro rooting was also achieved by pulse treatment with 300 μM IBA for 10 min. Rooted shoots were transplanted in plastic pots containing garden soil (with 90 % survival rate), where they grew well and attained maturity. Regenerated plants were phenotypically and cytologically normal.
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Abbreviations
- IAA:
-
indole-3-acetic acid
- IBA:
-
indole-3-butyric acid
- BM:
-
basal medium
- NAA:
-
α-naphthalene acetic acid
- MS:
-
Murashige and Skoog
- PGR:
-
plant growth regulator
- TDZ:
-
thidiazuron
References
Agrawal, S., Chandra, N., Kothari, S.L.: Shoot tip culture of pepper for micropropagation.-Curr. Sci. 57: 1347–1349, 1988.
Agrawal, S., Chandra, N., Kothari, S.L.: Plant regeneration in tissue cultures of pepper (Capsicum annuum L. cv. Mathania).-Plant Cell Tissue Organ Cult. 16: 47–55, 1989.
Anis, M.: Cytological investigation in genus Citrus. Inter-and intra-specific variation at meiosis in some species and varieties.-Phytomorphology 48: 349–356, 1998.
Cheepala, S.B., Sharma, N.C., Sahi, S.V.: Rapid in vitro regeneration of Sesbania drummondii.-Biol. Plant. 48: 13–18, 2004.
Christopher, T., Rajam, M.V.: In vitro clonal propagation of Capsicum spp.-Plant Cell Tissue Organ Cult. 38: 25–29, 1994.
Çöçü, S., Uranbey, S., İpek, A., Khawar, K.M., Sarihan, E.O., Kaya, M.D., Parmaksiz, İ., Özcan, S.: Adventitious shoot regeneration and micropropagation in Calendula officinalis L.-Biol. Plant. 48: 449–451, 2004.
Dolendro, S.N., Sahoo, L., Bhalla, S.N., Jaiwal, P.K.: The effect of TDZ on organogenesis and somatic embryogenesis in pigeon pea (Cajanus cajan L. Mill sp).-Plant Sci. 164: 341–347, 2003.
Franck-Duchenne, M., Wang, Y., Tahar, S.F., Beachy, R.N.: In vitro stem elongation of sweet pepper in media containing 24-epi brassinolide.-Plant Cell Tissue Organ Cult. 53: 79–84, 1998.
Hyde, C., Phillips, G.C.: Silver nitrate promotes plant regeneration of Chile pepper (Capsicum annuum L.) via organogenesis.-In Vitro cell. dev. Biol. Plant 32: 72–80, 1996.
Kintzios, S., Drossopoulos, J.B., Shortsianitis, E., Peppes, D.: Induction of somatic embryogenesis from young fully expanded leaves of chilli pepper (Capsicum annuum L.): effect of leaf position, illumination and explant pretreatment with high cytokinin concentrations.-Scientia Hort. 85: 137–144, 2000.
Malabadi, R.B., Mulgund, G.S., Natraja, K.: Efficient regeneration of Vanda coerulea, an endangered orchid using thidiazuron.-Plant Cell Tissue Organ Cult. 76: 289–293, 2004.
Manoharan, M., Vidya, C.S.S., Sita, G.L.: Agrobacterium mediated genetic transformation in hot chilli (Capsicum annuum L var. Pusa Jwala).-Plant Sci. 131: 77–83, 1998.
Murashige, T., Skoog, F. A revised medium for rapid growth and bioassays with tobacco tissue cultures.-Physiol Plant 15: 473–497, 1962.
Ochoa-Alejo, N., Ramirez-Malagon, R.: In vitro chilli pepper Biotechnology.-In Vitro cell. dev. Biol. Plant 37: 701–729, 2001.
Steinitz, B., Wolf, D., Matzevitch-Josef, T., Zelcer, A.: Regeneration in vitro and genetic transformation of pepper (Capsicum spp.). The current state of art.-Capsicum and Egg Plant Newslett. 18: 9–15, 1999.
Valera-Montero, L.L., Ochoa Alejo, N.: A novel approach for chili pepper (Capsicum annuum L.) plant regeneration: Shoot induction in rooted hypocotyls.-Plant Sci. 84: 215–219, 1992.
Venkataiah, P., Christopher, T., Subhash, K.: Thidiazuron induced high frequency adventitious shoot formation and plant regeneration in Capsicum annuum L.-Plant Biotechnol. 5: 245–25, 2003.
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Ahmad, N., Siddique, I. & Anis, M. Improved plant regeneration in Capsicum annuum L. from nodal segments. Biol Plant 50, 701–704 (2006). https://doi.org/10.1007/s10535-006-0110-5
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DOI: https://doi.org/10.1007/s10535-006-0110-5