Abstract
A protocol of high frequency shoot organogenesis and plant establishment from stem derived callus has been developed for Tylophora indica (Burm. f.) Merrill. - an endangered medicinal plant. Callus was developed on Murashige and Skoog (MS) medium supplemented with 10 μM 2,4,5-trichlorophenoxy acetic acid (2,4,5-T). Multiple shoot induction was achieved from the surface of the callus after transferring onto shoot induction medium. The highest rate (80 %) of shoot multiplication was achieved on MS medium containing 5.0 μM kinetin. The developed shoots rooted best on half-strength MS medium supplemented with 0.5 μM indole-3-butyric acid (IBA). The in vitro raised plantlets with well developed shoot and roots were acclimatized successfully and grown in greenhouse.
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Abbreviations
- BA:
-
6-benzyladenine
- 2,4-D:
-
2,4-dichlorophenoxy acetic acid
- IAA:
-
indole-3-acetic acid
- IBA:
-
indole-3-butyric acid
- MS:
-
Murashige and Skoog
- NAA:
-
α-naphthalene acetic acid
- 2,4,5-T:
-
2,4,5-trichlorophenoxy acetic acid
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Faisal, M., Anis, M. An efficient in vitro method for mass propagation of Tylophora indica . Biol Plant 49, 257–260 (2005). https://doi.org/10.1007/s10535-005-7260-8
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DOI: https://doi.org/10.1007/s10535-005-7260-8