Abstract
We describe the multi-step regeneration system of medicinal plant Schisandra chinensis (Turcz.) Baill. The seeds were pre-treated with 0.005 μM thidiazuron. Subsequently the zygotic embryos of the early heart stage were cultured on medium with 50 μM of 2,4-dichlorophenoxyacetic acid (2,4-D) and after three weeks the embryogenic calli were transferred to a medium with 10 μM of 2,4-D and 4 μM of 6-benzyladenine and were sub-cultured at the 4-week intervals. Abscisic acid (30 μM) and polyethyleneglycol (3 %) significantly influenced the synchronization of development of the somatic embryos (SEs) to the globular stage. The following culture on a medium without growth regulators resulted in full developed cotyledonary stage SEs. Indole-3-butyric acid (0.05 μ) contributed to their rapid conversion to plantlets.
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Abbreviations
- ABA:
-
abscisic acid
- AC:
-
activated charcoal
- BA:
-
6-benzyladenine
- 2,4-D:
-
2,4-dichlorophenoxyacetic acid
- IBA:
-
indole-3-butyric acid
- MS medium:
-
Murashige and Skoog medium
- PEG:
-
polyethyleneglycol 4000
- SEs:
-
somatic embryos
- TDZ:
-
thidiazuron
- WV5:
-
Westvaco medium
- ZEs:
-
zygotic embryos
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This experimental work was supported by project No. 521/02/1129 of the Grant Agency of the Czech Republic.
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Smiskova, A., Vlasinova, H. & Havel, L. Somatic embryogenesis from zygotic embryos of Schisandra chinensis . Biol Plant 49, 451–454 (2005). https://doi.org/10.1007/s10535-005-0027-4
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DOI: https://doi.org/10.1007/s10535-005-0027-4