Abstract
In vitro propagation protocol for Haemaria discolor (Ker) Lindl. var. dawsoniana by artificial cross-pollination and asymbiotic germination of seeds has been developed. Fruit set (100 %) was obtained when the pollinia and ovules of various aged flowers were used for pollination. In vitro germination of seeds obtained from capsules of various ages was achieved on half-strength Murashige and Skoog’s (MS) medium supplemented with 3 % sucrose and 0.85 % agar. The germinated seedlings were cultured on half-strength MS medium with 0.2 % activated charcoal, 8 % banana homogenate, 0.1 mg dm−3 1-phenyl-3-(1,2,3-thiadiazol-5-yl)urea (TDZ) and 1 mg dm−3 α-naphthaleneacetic acid (NAA). Ninety-six percent of plantlets survived after hardening in greenhouse.
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Abbreviations
- TDZ:
-
1-phenyl-3-(1,2,3-thiadiazol-5-yl)urea
- NAA:
-
α-naphthaleneacetic acid
- MS:
-
Murashige and Skoog’s medium
- DAF:
-
days after flowering
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This research was supported by grant (91AS-3.1.1-CI-C3) from the Council of Agriculture, Executive Yuan of Taiwan and grants (NSC-89-2317-B055-002 and NSC-91-2317-B324-001) from the National Science Council of Taiwan. This paper is Agricultural Research Institute Contribution No. 2158.
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Shiau, YJ., Nalawade, S.M., Hsai, CN. et al. Propagation of Haemaria discolor via in vitro seed germination. Biol Plant 49, 341–346 (2005). https://doi.org/10.1007/s10535-005-0005-x
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DOI: https://doi.org/10.1007/s10535-005-0005-x