Abstract
Globally, hypoxic areas (<63 mmol O2 m−3) in coastal waters are increasing in number and spatial extent. One of the largest coastal hypoxic regions has been observed during the summer in the bottom-water of the Louisiana continental shelf. The shelf receives the sediments, organic matter, and nutrients exported from the Mississippi River watershed, and much of this material is ultimately deposited to the sea floor. Hence, quantifying the rates of sediment-water dissolved inorganic carbon (DIC), oxygen (O2), and nutrient fluxes is important for understanding how these processes relate to the development and maintenance of hypoxia. In this study, the sediment-water fluxes of DIC, O2, nutrients, and N2 (denitrification) were measured on the Louisiana shelf during six cruises from 2005 to 2007. On each cruise, three to four sites were occupied in or directly adjacent to the region of the shelf that experiences hypoxia. DIC fluxes, a proxy for total sediment respiration, ranged from 7.9 to 21.4 mmol m−2 day−1 but did not vary significantly either spatially or as a function of bottom-water O2 concentration. Overall, sediment respiration and nutrient flux rates were small in comparison to water-column respiration and phytoplankton nutrient demand. Nitrate fluxes were correlated with bottom-water O2 concentrations (r = 0.69), and there was evidence that decreasing O2 concentrations inhibited coupled nitrification-denitrification. Denitrification rates averaged 1.4 mmol N m−2 day−1. Scaled to the area of the shelf, the denitrification sink represented approximately 39% of the N load from the Mississippi River watershed. The sediment-water fluxes reported from this study add substantial information on the spatial and temporal patterns in carbon, O2, and nutrient cycling available for the Louisiana continental shelf and, thus, improve the understanding of this system.
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Introduction
Hypoxic areas in coastal waters have increased globally in number and spatial extent (Diaz and Rosenberg 2008). The seasonally hypoxic region of the Louisiana shelf is typical of the global distribution of hypoxic regions, being located adjacent to a watershed highly altered by human activities and discharging large nutrient loads. In combination with the eutrophication, the Louisiana shelf is susceptible to hypoxia (O2 < 63 mmol m−3) in bottom waters owing to the water-column stratification that develops after Mississippi River spring discharge (Wiseman et al. 1997). Following the onset of stratification, an expansive area of hypoxia is formed in the late spring and summer. The average hypoxic area from 1985 to 2007 was 13,500 km2 (Rabalais et al. 2007).
Over the last several decades there has been active research exploring how riverine delivery of freshwater, nutrients, and organic matter relate to the extent and timing of hypoxia formation on the Louisiana shelf (reviewed by Rabalais et al. 2007; Dagg et al. 2007; Bianchi et al. 2010). By consensus, a primary mechanism for the development of hypoxia on this shelf is nutrient enhanced primary production that is subsequently respired in bottom waters. However, the myriads of water-column and sediment biogeochemical processes that interact to affect O2 concentrations are complex and there are few measurements and large uncertainties for the magnitudes of carbon, O2, and nutrient cycling processes across the Louisiana shelf; especially in the sediments (Dagg et al. 2007; Bianchi et al. 2010).
Though sediment-water fluxes of carbon, O2, nitrogen, phosphorus, and silica have been described as potentially important sinks and sources on the Louisiana continental shelf (Eldridge and Morse 2008), measurements have been reported for only a few sites located on the eastern shelf, either beneath or adjacent to the Mississippi River plume (Gardner et al. 1993; Miller-Way et al. 1994; Morse and Rowe 1999; Rowe et al. 2002). There are no reported sediment-water flux rates from the western shelf (west of the Atchafalaya River outlet, >91.5ºW), which annually has an expansive area of hypoxia that may equal or exceed the hypoxic area on the eastern shelf (Rabalais et al. 2002).
The role of sediments as a nitrogen sink on this shelf is largely unknown, but is likely to be significant through denitrification. Denitrification has been estimated to remove 30–60% of the annual nitrogen inputs to continental shelf systems (Seitzinger et al. 2006). This loss of fixed nitrogen is potentially an important moderator of primary production. Hypoxic conditions, though, have been observed to alter nitrogen fluxes by limiting coupled nitrification–denitrification and enhancing fluxes of NH4 + from the sediment (McCarthy et al. 2008; Middelburg and Levin 2009). Hence, under hypoxic conditions nitrogen may recycle many more times than under normoxic (100% O2 saturation) conditions, and, thus, may support elevated rates of primary production across the shelf. For the Louisiana shelf, and marine systems in general, there are limited measurements and understanding of the strengths of such feedback mechanisms.
Spatial zones of similarity in sediment-water fluxes across the Louisiana shelf have been postulated due to differing degrees of freshwater influence, and, subsequently, variation in water-column primary production and organic matter deposition (Rowe and Chapman 2002; Dale et al. 2010). The zones of similarity for the Louisiana shelf have been described as a river plume region proximal to the discharge of the Mississippi and Atchafalaya Rivers (zone 1), an intermediate region adjacent to the plume with high rates of primary production (zone 2), a distant region with higher salinity and lower primary production (zone 3), and a region representing the inshore coastal current (zone 4). As in any dynamic system, zonal boundaries may not be fixed in time or space, but are useful for envisioning how the magnitudes of various biogeochemical processes influence the system. Identification of spatial patterns in sediment and water-column processes for the Louisiana shelf will improve the quantitative understanding of the processes that act as recyclers and sinks of nutrients and organic matter delivered by the Mississippi River watershed.
Materials and methods
The objectives of this study were to measure sediment-water fluxes of carbon, oxygen, nitrogen, phosphorus, and silica at sites distributed across the shelf, and to explore factors driving variability in sediment processes. Potential factors include riverine freshwater and nutrient loads, bottom-water O2 concentrations, and water-column phytoplankton biomass and production. An emphasis of this study was to evaluate whether sediment-water fluxes were spatially coherent with the four zones of similarity (Rowe and Chapman 2002; Dale et al. 2010).
Study sites
During six cruises from 2005 to 2007, 23 stations were occupied in four study sites whose characteristics were consistent with those of the four zones of similarity (Dale et al. 2010). Site Z01 (Fig. 1) was located adjacent to Southwest Pass, the largest of the passes from the lower Mississippi River to the Louisiana continental shelf, in a region that has been identified as the river plume (Green et al. 2006). Z02 was located to the west of the Mississippi River plume. Site Z03 was located on the western Louisiana shelf in a region of high salinity and low chla concentration (Lehrter et al. 2009). Sites Z02 and Z03 both frequently experience hypoxia (Rabalais et al. 2002). Site Z04 was located in a region that is in the inshore region of the Louisiana coastal current (Wiseman et al. 1997). Z01, Z02, and Z03 had similar depths (19–22 m), whereas Z04 had shallower depths (5–8 m). During cruises when multiple stations were located within a site, average values of measurements (±standard error) were used.
River discharge and hydrographic data
Daily discharge data for the lower Mississippi and Atchafalaya Rivers were obtained from the US Army Corps of Engineers (gauge IDs 01100 and 03045, respectively) for the years 2005 to 2007. Estimated monthly nutrient loading data for the rivers were obtained for 2005–2007 from the US Geological Survey (Aulenbach et al. 2007). The discharge and nutrient data were used to evaluate the potential role of river forcing on sediment-water fluxes.
Sampling on the Louisiana continental shelf was performed from the OSV Bold for the five cruises during 2006 and 2007 and from the R/V Longhorn during the March 2005 cruise. At each site, vertical profiles of temperature, salinity, dissolved O2, photosynthetically active radiation (PAR), and fluorescence were collected with a Sea-bird 911 CTD. Bottom-water samples for O2 and nutrients were obtained from waters overlying sediment cores collected at the sites (see below for details on core collection).
Sediment-water fluxes
Sediment-water fluxes of DIC, O2, N2, and nutrients were measured in the dark with sediment chamber incubation methods modified from Cowan and Boynton (1996). During the five cruises in 2006 and 2007, sediment cores were collected with a hydraulically-dampened multi-corer (Ocean Instruments MC-400). The multi-corer was configured to collect four cores in Plexiglas chambers (10 cm ID × 40 cm). Following deployment and retrieval of the corer, sediments were inspected to ensure that the sediment surface layer was undisturbed. Generally, sediment cores passing inspection had visually apparent burrows and feeding tubes of benthic fauna at the sediment surface and clear overlying water. The in-situ water overlying the sediments was retained by the multi-corer (average depth of sediment from all cores was 18 cm). In March 2005, sediments were collected with a 0.25 m2 box corer and sub-cored with the Plexiglas chambers. The chambers were gently pushed into the sediment surface, being careful not to disturb the surface sediment layer, and then excavated from the box corer. During retrieval of box cores, much of the bottom-water overlying the sediments drained from the surface of the core and, thus, was replenished with bottom-water carefully siphoned into the chambers until they were filled.
At each site, six sediment cores were collected in individual chambers; three were used for measurement of N2 and O2 fluxes and three were used for measurement of DIC and nutrient fluxes. Each triplicate set was submersed in a dark incubator filled with site bottom-water collected with a pump deployed from the ship. In addition to the triplicates, one chamber containing only bottom-water was incubated as a control. The incubator was maintained at ambient bottom temperatures (±0.5°C) using a thermostatically-controlled recirculating water bath. After submersion in the incubator, the sediment chambers were pre-incubated for at least 4 h while exposed to the large reservoir of site bottom-water in the incubator. This process allowed the sediments to equilibrate to the incubator conditions, while maintaining ambient bottom-water O2 and nutrient concentrations overlying the cores through diffusional exchanges with the large volume (~0.5 m3) of site bottom-water in the incubator.
Following the equilibration period, Plexiglas caps were fitted to seal the chambers. The caps were put in place while submerged in the incubator to avoid the introduction of gas bubbles. Once the caps were in place, the chambers remained closed until the end of the incubation. Within a chamber, the water overlying the sediment was mixed by a 2.5 cm magnetic stir bar suspended from the cap. The magnetic stir bars for the three sediment chambers and the one control (i.e. bottom-water only) chamber were stirred (~45 RPM) simultaneously by an external motor that turned a circular plate mounted with four magnets.
Sampling of the chambers without removing the caps was facilitated by inlet and outlet ports plumbed through the core cap. The inlet port was gravity-fed through tubing from a 5-l carboy filled with site bottom-water. Collecting an overlying water sample from the chamber entailed opening the inlet port to allow a volume of bottom-water into the chamber and thereby displace an equal volume through the outlet port. Keeping the chambers sealed throughout the incubation reduced the potential for contamination by atmospheric gases, especially N2, and solutes that may have otherwise occurred with invasive sampling. Samples were collected approximately every 2 h (four to seven samples) during 8–16 h incubations.
Dissolved N2, O2, and Ar samples were collected in duplicate in 7-ml glass test tubes. Samples were fixed with 20 μl of 5% HgCl2, tightly sealed with ground glass stoppers, and stored submerged in chilled seawater for 1–2 weeks until analyzed. Laboratory tests indicated the glass-stoppered test tubes maintained constant N2, O2, and Ar concentrations for several weeks to a month (data not shown). Dissolved gas analyses were conducted on a Pfeiffer Prisma quadrupole mass spectrometer fitted with a membrane inlet (Kana et al. 1994).
DIC analyses were conducted shipboard, immediately after collection, on unfiltered samples with a Shimadzu TOC-5000 carbon analyzer calibrated with a certified seawater DIC standard (Scripps Institute of Oceanography). Ammonium (NH4 +) analyses were performed shipboard on filtered (GF/F) samples using the fluorometric method described by Holmes et al. (1999). Samples for nitrate + nitrite (NO3 −), dissolved inorganic phosphorus (DIP), and silicate (SiO2) were filtered through GF/F filters and stored at −70 °C until analysis. NO3 −, NO2 −, DIP, and SiO2 concentrations were measured (APHA 1989) on a continuous flow auto-analyzer (Astoria-Pacific).
The sediment-water flux (mmol m−2 day−1) of a constituent was calculated as
where \( {\text{m}}_{\text{sediment}} \) was the rate of change in concentration for waters overlying sediment cores obtained from linear regression of the sample concentrations versus time, \( {\text{m}}_{\text{bottom-water}} \)was the rate of change in concentration for the bottom-water control, and \( {\text{z}}_{\text{overlying\;water}} \) was the water depth (m) in the Plexiglas chamber. Overlying water concentrations were not corrected for refill water concentrations as the volume of refill water was never more than 2% of the total overlying water volume, and, therefore, was assumed to be a small contribution to the overall concentration trend versus time. Confidence intervals were used to evaluate whether a slope was significantly different from zero (α = 0.20). Slopes not significantly different from zero were set equal to zero. Generally, a slope not significantly different from zero resulted from small changes in concentration during the incubation. Positive fluxes indicated a net efflux out of the sediments.
Sediment bulk analyses
Sediment porosity, organic carbon, organic nitrogen, organic phosphorus, and chla were measured on surficial sediments (top 1–2 cm) that were frozen (−20°C) until laboratory analysis. From the box corer, surface sediment samples were collected from unused portions of the box core. From the multi-corer, surface sediment samples were obtained from another set of cores collected at the same time as cores used for flux measurements. Porosity was calculated as dry bulk density divided by the particle density where particle density was taken to be 2.6 g cm−3. For chemical analyses of the sediments, sediments were dried, milled to a powder, and fumed with HCl to volatilize inorganic carbon. Sediment organic carbon and nitrogen concentrations were measured following high temperature combustion on an Elementar Vario EL elemental analyzer. Sediment organic phosphorus concentrations were calculated as the difference of sediment total phosphorus and sediment inorganic phosphorus determined following the method of Aspila et al. (1976). Chla concentrations were determined fluorometerically on extracts obtained with 0.5 g sediment in 10 ml of 90% buffered methanol (Welschmeyer 1994).
Phytoplankton biomass and production rates
Water-column samples of chla were collected at multiple depths, surface and bottom at a minimum, and analyzed fluorometrically (Welschmeyer 1994). Phytoplankton photosynthesis-irradiance (PE) experiments were conducted at each station. During March 2005 one PE experiment per station was performed. On the 2006 and 2007 cruises, the sites were occupied for over a diel cycle, thus allowing multiple (3–7) PE experiments to be performed at each site. The PE methods and models used to estimate primary production rates have been presented in detail elsewhere (Lehrter et al. 2009). Phytoplankton biomass and production rates were compared to sediment-water fluxes to identify the degree of potential coupling between primary production and sediment-water fluxes of carbon, oxygen, and nutrients.
Statistical analyses
Analysis of variance (ANOVA) was used to assess differences across sites and across cruises. Least squares regresssions were used to estimate sediment-water flux rates and to evaluate relationships between potential forcing factors and flux rates.
Results
Mississippi river discharge and shelf hydrographic conditions
Sampling occurred over variable Mississippi and Atchafalaya River discharge regimes (Fig. 2). The March 2005, April and June 2006, and August 2007 cruises occurred on the falling limbs of peaks in the discharge hydrograph. A period of sustained low discharge preceded the September 2006 cruise (July–September mean discharge = 7,993 m3 s−1), while a period of sustained high discharge preceded the April 2007 cruise (February–April mean discharge = 23,967 m3 s−1).
Vertical profiles of water-column temperature, salinity, and density (Fig. 3) varied among the sites, though bottom-water salinity was similar (>35). Z01 had the lowest surface salinities (average = 22.7) and shallowest pycnoclines (average = 6.8 m). Z02 generally had a well-mixed surface layer (average surface salinity = 29.7) and a strong pycnocline at a depth of approximately 10 m. In the late summer, however, the vertical profiles at Z02 were more similar to those observed at Z03, which generally had higher salinity in the surface layer (average surface salinity = 32.2) and an average pycnocline depth of 13 m. Z04, located in the coastal current, had an average surface salinity of 28.9, and had a shallow pycnocline at about 2.1 m depth. Bottom-water temperatures across all sites ranged from 20.0°C in the spring to 30.0°C in the summer.
Water-column O2 concentration profiles (data not shown) were generally uniform in the surface-mixed layer and decreased below the pycnocline. Hypoxic conditions in the bottom waters were encountered in April 2006 at Z01 and Z02, June 2006 at Z02, September 2006 at Z02, and August 2007 at Z02 and Z03. The lowest O2 concentrations (12–16 mmol m−3) were encountered in August 2007. Over the course of the study, average bottom-water O2 concentrations at the sites were ordered from minimum to maximum as Z02 < Z03 < Z01 < Z04 (Table 1).
Average nutrient concentrations in the bottom waters (Table 1), were typically highest at Z02 where the NO3 − and DIP concentrations were 7.0 and 1.2 mmol m−3, respectively. Bottom-water NO3 − concentrations at Z01 (average = 6.9 mmol m−3) and Z04 (average = 5.5 mmol m−3) were also similar to those observed at Z02. Z03 typically had the lowest bottom-water NO3 − (average = 2.2 mmol m−3). Highest bottom-water NH4 + levels were observed at Z04 (average = 4.9 mmol m−3).
Sediment characteristics
Porosity in the surface sediments were similar at Z01 and Z04 with averages (±SE) being 0.6 (±0.1) and 0.5 (±0.01), respectively (data not shown). Z02 and Z03 had higher average porosity of 0.8 (±0.04) and 0.8 (±0.03), respectively. Sediment carbon, nitrogen, and phosphorus concentrations (Table 1) were largest at Z02 and Z03, whereas chla concentrations (Table 1) at the sites were not statistically different due to high variability across cruises.
Sediment-water fluxes
There were no significant differences in fluxes by cruise (ANOVA) despite high variability in river discharge preceding each cruise. Also, site-specific regression analyses between sediment-water fluxes, river discharge, and NO3 − loads did not yield significant relationships. The lack of significance may be partially attributed to low degrees of freedom (df = 2–5) for the regression statistics.
DIC fluxes ranged from 7.9 to 21.4 mmol m−2 day−1 (Fig. 4), but did not exhibit differences by site (ANOVA, P = 0.65) owing to high variability. Site average DIC fluxes ± standard error were 18.7 ± 1.8, 14.6 ± 1.4, 15.8 ± 2.1, and 19.8 ± 1.5 at Z01, Z02, Z03, and Z04, respectively. O2 fluxes ranged from 0 to −23.9 mmol m−2 day−1 (Fig. 4), and were significantly different among sites (ANOVA, P = 0.04). Largest O2 fluxes into the sediments occurred at Z01 and Z04 (average = −14.3 and −15.5 mmol m−2−day−1, respectively) and lowest O2 fluxes occurred at Z02 and Z03 (average = −2.4 and −7.8 mmol m−2−day−1, respectively).
Sediment NO3 − fluxes ranged from −1.01 to 1.03 mmol m−2 day−1 (Table 2) and varied by site (ANOVA, P = 0.09). Z01 and Z04 sediments were, on average, net sources of NO3 − with average fluxes of 0.19 and 0.36 mmol m−2 day−1, respectively. During 4 of the 6 cruises Z02 was a net sink of NO3 − and had an overall average flux of −0.30 mmol m−2 day−1. Sediment NH4 + fluxes ranged from −0.17 to3.84 mmol m−2 day−1 (Table 2), but did not vary by site (ANOVA, P = 0.46). Most of the NH4 + fluxes were directed out of the sediments, but small fluxes of NH4 + into the sediment (−0.07 to −0.17 mmol m−2 day−1) were observed at Z04 during March 2005 and Z03 during April 2006 and April 2007.
Sediment DIP fluxes ranged from −0.19 to 0.26 mmol m−2−day−1 (Table 2), but did not vary by site (ANOVA, P = 0.71). DIP effluxes from the sediment were most common (14 of 18 observations). Sediment Si fluxes ranged from 0.23 to 4.44 mmol m−2 day−1 (Table 2), but were not statistically different across sites (ANOVA, P = 0.53).
Sediment N2 fluxes ranged from 0 to 3.03 mmol m−2 day−1 (Table 2). N2 fluxes were highly variable, but most of the observations (11 of 18) fell within a small range (0 to 0.49 mmol m−2 day−1). Observations of N2 fluxes greater than 1 mmol m−2 day−1 occurred for 5 of 18 observations. As a result of the high variability, average N2 fluxes did not vary significantly by site (ANOVA, P = 0.38). The N2 flux average for the study was 0.70 mmol m−2 day−1 (SE = 0.04, n = 18).
Relationships among sediment-water fluxes and other variables
Under normoxic conditions, DIC and O2 fluxes were not significantly different from one another (Fig. 4). However, under hypoxic conditions DIC fluxes were much larger than O2 fluxes. DIC fluxes did not covary with bottom-water O2 concentrations (P = 0.9), and the magnitude of DIC fluxes during hypoxic events (average = 16.3 mmol m−2 day−1) did not differ from DIC fluxes measured under normoxic conditions (average = 16.6 mmol C m−2 day−1). In contrast, sediment O2 flux rates strongly covaried with bottom-water O2 concentrations (r = −0.62, P = 0.006, n = 18) with rates approaching zero when overlying O2 concentrations were depleted (Fig. 5). This was the case most often at site Z02 which typically had the lowest bottom-water O2 concentrations (average = 59 mmol m−3) and the smallest O2 fluxes (average = −2.4 mmol m−2 day−1). Z01 and Z04 had the highest bottom-water O2 concentrations (Table 1) and the highest O2 fluxes (Fig. 4). The DIC/O2 flux ratio, an indicator of the sediment re-oxidation efficiency, also varied as a function of O2 concentration (Fig. 5).
NO3 − fluxes were positively correlated with bottom-water O2 concentrations (Fig. 5). NO3 − fluxes were also significantly correlated with NH4 + fluxes (r = −0.69, P = 0.001, n = 18) and N2 fluxes (r = 0.54, P = 0.02, n = 17). A significant correlation between NO3 − flux and bottom-water NO3 − concentration was not observed (P = 0.90). A multiple linear regression model, NO3 − flux ~ f(O2, NH4 + flux, N2 flux), explained a large fraction of the variability in NO3 − flux (R 2 = 0.83, Fig. 6). N2 fluxes were not directly correlated with bottom-water O2 concentrations, sediment O2 fluxes, or bottom-water NO3 − concentrations (P = 0.39, 0.76, and 0.50, respectively).
Site water-column chla measurements (described below) were correlated with both DIC (r = 0.55, P = 0.04, n = 15) and N2 (r = 0.50, P = 0.04, n = 15) fluxes. DIP fluxes were significantly correlated to sediment surface chla concentration (r = 0.61, P = 0.02, n = 15). None of the fluxes were significantly correlated with temperature.
Water-column phytoplankton biomass and primary production
Chla concentrations averaged over the euphotic depth (i.e., the depth of 1% surface light) were 10.0, 4.9, 1.4, and 13.3 mg m−3 at Z01, Z02, Z03, and Z04, respectively. Z03 and Z04 had chla concentrations significantly higher than Z02 and Z03 (ANOVA, P = 0.05). Phytoplankton primary production rates (Fig. 7) ranged from 27 to 235 mmol C m−2 day−1. Primary production rates at Z01 in April and June 2006 (235 and 161 mmol Cm−2 day−1, respectively) were significantly greater than at Z02 and Z03. The lowest salinities (18.0 and 22.6, respectively) were observed at Z01 during these dates. Rates of primary production at Z04 (210 mmol C m−2 day−1) were significantly greater than at Z02 and Z03 during April 2007, and corresponded with the lowest salinity (29.4) observed on that cruise. Z02 and Z03 had statistically similar primary production rates on all 5 cruises.
As a result of low light attenuation and shallow pycnocline depths, significant fractions of water-column primary production occurred beneath the pycnocline. At Z02, Z03, and Z04 the percentage of primary production occurring beneath the pycnocline averaged 26, 30, and 50%, respectively. In contrast, at Z01 where light attenuation was highest, only 7% of the primary production, on average, occurred beneath the pycnocline. The percentage of primary production below the pycnocline is likely to be a conservative estimate for Z02 and Z03 which were located in regions of the shelf with low light attenuation, hence large amounts of light reaching the bottom (Lehrter et al. 2009), and, thus, potential for benthic primary production (Dortch et al. 1994).
Sediment-water fluxes in comparison to water-column primary production
On average, the fraction of carbon fixation by water-column primary production accounted for by the sediment DIC fluxes was 12, 19, 23, and 24% at stations Z01, Z02, Z03, and Z04, respectively. Site average sediment DIN and DIP fluxes, when positive, could potentially supply 2–7 and 4–11%, respectively, of the water-column integrated primary production nutrient demand calculated according to the Redfield ratio (Table 3). Assuming the water-column phytoplankton were dominated by diatoms (J. Kurtz unpublished data) and that diatoms require Si in the same ratio as nitrogen (i.e. Redfield), sediment Si fluxes could supply 9–26% of the phytoplankton demand. For the sub-pycnocline portion of the water-column, sediment nutrient fluxes potentially provided a large fraction of the nutrients required by phytoplankton (Table 3). On average, DIN fluxes could provide 43–66% and DIP fluxes could provide 5–108% of the sub-pyncocline phytoplankton demand. The observations of nutrient fluxes directed into the sediments (Table 2) indicated that at times the sediment community exerted a nutrient demand equal to or greater than the water-column demand.
Discussion
Prior to this study, published DIC and nutrient flux rates were limited to cruises from July 1990, April 1992, and August 1994 (Gardner et al. 1993; Miller-Way et al. 1994; Rowe et al. 2002). These studies were all conducted at sites in the vicinity of the Mississippi River plume on the eastern shelf. The only published estimates of denitrification rates were from the July 1990 cruise, which were estimated indirectly by stoichiometry from nutrient and O2 flux rates (Gardner et al. 1993). Sediment O2 flux measures were recently reported for 10 cruises from 2003 to 2007 (Murrell and Lehrter 2011). The O2 fluxes reported herein from 2005 to 2007 were measured at the same locations as in Murrell and Lehrter (2011). However, the O2 flux data presented in this study are independent observations as the measurements were made on separate sets of triplicate cores using different methods.
Methodological considerations for sediment-water fluxes
Sediment-water flux rates determined from in situ chamber measurements and shipboard chamber measurements have been shown to be equivalent (Miller-Way et al. 1994). Sediment-water flux measurements in chambers, however, are susceptible to methodological artifacts that may affect observations. These artifacts arise from the sensitivity of fluxes to bottom-water O2 concentrations, hydrodynamics, and the activity of benthic fauna. Bottom-water O2 concentrations strongly regulate the depth of O2 penetration depth in the sediments and, thus, the sediment-water O2 flux rates (Cai and Sayles 1996). Bottom-water O2 also affects the fluxes of NH4 +, NO3 −, and N2 by altering nitrification–denitrification rates (McCarthy et al. 2008; Middelburg and Levin 2009) and the fluxes of DIP through iron sorption and desorption of phosphate under oxic and anoxic conditions, respectively (Roden and Edmonds 1997). Altered hydrodynamics within chambers may serve to increase or decrease the transport of solutes into the sediments and, thus, affect their exchange rates (Glud 2008). Finally, chambers may under-represent benthic fauna that actively irrigate and enhance diffusion into the sediments through their burrows and that stimulate microbial activity through their metabolic activities (e.g. Aller and Aller 1986; Pelegri et al. 1994). Researchers using chambers should evaluate how these potential artifacts affect their measured rates.
In the present study, 12–16 h incubations were employed to determine sediment-water fluxes. As a consequence of these incubation times, on average there was a 36% decrease in O2 concentration over the incubation period. It has been suggested that O2 changes of greater than 10–15% from initial conditions will induce a non-linear change in O2 flux rates (Glud 2008), which may affect other flux rates based on their dependence on O2. In the present study, however, the linear approximation used to calculate the rate of change in the chamber incubations (i.e. \( {\text{m}}_{\text{sediment}}, \) Eq. 1) appeared to be a robust estimate as average R 2 from the linear regressions ranged from 0.7 to 0.9. It is recommended that for cases, such as encountered here, where the O2 concentration is expected to drop significantly that time points be collected every 1–2 h to evaluate linearity.
Chamber alterations to hydrodynamics are also known to affect flux rate measurements. Isolating the sediments with a chamber changes the water flow over the sediment surface. In permeable, sandy sediments, advective transport of solutes into sediment porewaters may be significant (Huettel and Webster 2001). However, in the impermeable, muddy sediments of the Louisiana shelf, 75% silt + clay on average (Murrell and Lehrter 2011), exchanges between bottom-water and sediment porewaters will be controlled by diffusive processes. Chamber effects on diffusion across the sediment-water interface primarily occur through changing the thickness of the diffusive boundary layer. The consensus from numerous studies of various chamber designs (reviewed by Glud 2008) is that this effect is likely to be small for sediments with an O2 penetration depth greater than a couple of mm. For the sites visited in this study, the average O2 penetration depth, calculated from Cai and Sayles (1996, Eq. 7A), was 2.3 mm (range = 0.3–5.5 mm). For perspective on how much a change in the thickness of the diffusive boundary layer might affect the average rates reported herein, a modeling study of sediments, which had a range of O2 penetration depths from 0.6 to 4.6 mm, indicated that the reduction in thickness of the diffusive boundary layer from 900 to 0 μm enhanced the calculated annual O2 consumption rate by 10% (Glud et al. 2007).
Another consideration for chamber effects on hydrodynamics is that it is necessary to stir the waters overlying the sediments in order to eliminate concentration gradients. Stirring is typically done with a magnetic stir bar driven by an external motor turning a magnet, and may enhance solute exchange with the sediments by advective or diffusive, i.e. reducing or eliminating the diffusive boundary layer, processes. To our knowledge, there have not been any studies that have quantified the impact of stirring rates on sediment-water fluxes for muddy sediments. In a study of sediment-water exchanges in sandy sediments it was observed that O2 and DIC fluxes were similar for stirring rates < 50 RPM, but flux rates increased at higher stirring rates (Cook et al. 2007). The chambers in the present study were stirred at a rate of 45 RPM which was observed to maintain a mixed water-column in the chamber, and, based on the work by Cook et al. (2007), likely did not significantly alter sediment-water flux rates.
Bias in measured exchange rates may also occur if the benthic fauna are disturbed or undersampled. Benthic fauna affect sediment-water exchanges through irrigation and enancement of diffusion (Aller and Aller 1986) and are known to enhance denitrification rates (Pelegri et al. 1994). The direct impact of the fauna on fluxes was not evaluated in this study. However, burrows and organisms inhabiting them were visually identified in many of the chambers and the largest within site variability in sediment-water exchange rates (standard errors in Fig. 4; Table 2) occurred at sites where a sediment chamber captured one or more large burrow-dwelling organisms. Inclusion of benthic fauna in a sample is controlled by both the size of the chamber and the number of replicates. A simulation study of the effects of chamber size on calculated O2 flux rates, indicated that chambers with a radius of 5 cm had O2 exchange rate inaccuracies >25% (Glud and Blackburn 2002). Increasing the chamber size and the number of replicates reduced inaccuracies. Chambers with a radius of 10 cm and n = 3 (the radius and number of replicates used herein) had simulated inaccuracies of ~7 to 18%.
A known bias of flux estimates is the area of the sediment surface in the chamber. The sediment surface has topographic relief and has been observed to be 7–12% larger than the planar surface, resulting in a 1.14 to 1.25-fold adjustment to rates calculated assuming a flat surface (Røy et al. 2005). Further work is needed to assess how these potential artifacts and bias interact to alter chamber-based flux rate estimates in comparison to in situ rates.
Characterization of sediment-water fluxes on the Louisiana shelf
Although the cruises occurred over a wide range of river discharge, there were no significant relationships between fluxes and either river discharge, nutrient load, or nutrient concentrations. It is likely that river discharge and loads influenced sediment respiration to some degree, but the small number of observations may not have been sufficient to demonstrate this relationship. It is also likely that seasonal sediment transport events occurring with tropical storms (Goñi et al. 2006; Sampere et al. 2008) and winter cold fronts (Walker and Hammack 2000) serve to homogenize the sediments at the annual scale and thus obscure smaller temporal variations that would occur in response to seasonal river nutrient and organic matter fluxes.
DIC and O2 fluxes
In the dark, sediment DIC fluxes approximate net CO2 production and as such are a proxy for sediment respiration (Hopkinson and Smith 2005). DIC fluxes were generally greater than absolute values of O2 fluxes (Fig. 4) and DIC flux rates were insensitive to changes in overlying bottom-water O2 concentration (Fig. 5). The DIC/O2 flux ratio was was observed to increase as O2 concentrations were depleted (Fig. 5). This ratio reflects the efficiency of reoxidation of NH4 + and reduced iron, manganese, and sulfur. Hence, sites with high DIC/O2 flux ratios were accumulating a chemical oxygen debt because there was not enough O2 in the overlying water to efficiently reoxidize the reduced products of anaerobic metabolism. These results are consistent with anaerobic respiration being the dominant organic carbon mineralization pathway in coastal sediments and a large portion of the O2 flux into the sediment resulting from reoxidation of reduced species created as end products of anaerobic respiration (Jørgensen 1982; Sampou and Oviatt 1991; Aller et al. 1996). For example, nitrification, calculated as the sum of N2 and NO3 − fluxes (Fennel et al. 2009), potentially accounted for 24% of the sediment O2 demand in this study. A meta-analysis of nitrification in aquatic sediments determined a similar estimate of 17% (Fennel et al. 2009).
A previous study reported DIC fluxes averaging (±SE) 31.1 (±8.7) mmol m−2 day−1 at sites near Z01 and Z02 (Rowe et al. 2002). However, the fluxes measured by Rowe et al. (2002) were not corrected for changes that could be attributed to the bottom-water alone. For the data reported herein, if we neglect the DIC fluxes observed in the bottom-water controls, the average sediment + bottom DIC flux would be 23.9 (±1.9) mmol m−2 day−1, which is lower, but not significantly different than Rowe et al.’s (2002) average. Site average DIC fluxes measured in this study were not statistically different from one another (Fig. 4), but had a pattern where the highest average fluxes (19.8 and 18.7 mmol C m−2 day−1) occurred in the coastal current (Z04) and plume (Z01) regions and lower DIC fluxes (14.6 and 15.8 mmol m−2 day−1) occurred at sites Z02 and Z03. Average DIC fluxes across all cruises (16.6 mmol m−2 day−1) were similar to DIC fluxes from other continental shelf systems (Fennel et al. 2009).
Sediment-water O2 fluxes were highly correlated with bottom-water O2 concentrations (Fig. 5), consistent with the mechanistic description of O2 flux magnitude being dependent on O2 concentration (Cai and Sayles 1996). Square-root dependence between O2 flux and bottom-water O2 concentration has been hypothesized (Bouldin 1968) and shown to fit fine-scale porewater O2 profiles (Cai and Sayles 1996). However, a non-linear regression using a square-root function provided a poorer fit (R 2 = 0.21) than did the linear fit shown in Fig. 5.
The sediment O2 fluxes from this study were compared to those measured by Murrell and Lehrter (2011) to assess how different core handling and O2 measurements influenced the calculated flux values. The average (±SE) O2 flux for the present study was −8.7 (±1.8) mmol O2 m−2 day−1and was not statistically different than the average O2 flux of −12.3 (±2.6) mmol m−2 day−1 from Murrell and Lehrter (2011). Reported O2 fluxes from this shelf are similar to those reported from other continental shelf environments (Devol and Christensen 1993; Devol et al. 1997; Laursen and Seitzinger 2002).
N, P, and Si fluxes
Sediment NH4 + fluxes (Table 2) were similar to previous measurements for the Louisiana shelf. Rowe et al. (2002) reported NH4 + fluxes that ranged from 0.8 to 4.4 mmol m−2 day−1, Morse and Rowe (1999) observed NH4 + fluxes beneath the plume ranging from 2.6 to 4.2 mmol m−2 day−1 [though these data appear to be a subset of the Rowe et al. (2002) data set], and Gardner et al. (1993) measured NH4 + fluxes ranging from 0.3 to 1.3 mmol m−2 day−1. Sediment NO3 − fluxes (Table 2) differed from those reported by Rowe et al. (2002) who observed only negative NO3 − fluxes (range = −2 to −0.2 mmol m−2 day−1), but were similar to the NO3 − fluxes observed by Gardner et al. (1993); range −0.6 to 0.3 mmol m−2−day−1.
Sediment DIP fluxes (Table 2) were similar to the DIP fluxes previously reported for the Louisiana shelf ranging from −0.41 to 0.26 mmol m−2−day−1 (Miller-Way et al. 1994; Morse and Rowe 1999). The only previous Si fluxes reported for the Louisiana shelf averaged 6.6 mmol m−2 day−1 (Miller-Way et al. 1994), which was larger than the average rate observed in this study (1.7 mmol Si m−2 day−1).
A model developed by Eldridge and Morse (2008) estimated that sediment nitrogen fluxes could supply 25–60% of the water-column phytoplankton nutrient demand on the Louisiana continental shelf. Observations from the present study (Table 3) indicated that sediment-water fluxes of dissolved inorganic nitrogen generally supplied <10% of water-column nitrogen demand. The potential percent contributions of sediment DIP fluxes to water-column phytoplankton phosphorus demand were similarly small. However, in comparison to estimated sub-pycnocline nutrient demand by phytoplankton, sediment DIN, DIP, and Si fluxes could potentially supply a large percentage of the demand (Table 3). Thus, sediment nutrient fluxes were potentially important for primary production occurring beneath the pycnocline, which has been shown to be a significant fraction of water-column primary production across much of the shelf (Lehrter et al. 2009).
Denitrification
Site average denitrification rates ranged from 0.9 to 2.8 mmol N m−2 day−1 and were similar to previously published denitrification rates for this system. Gardner et al. (1993) reported rates of 0.5–1.1 mmol N m−2 day−1, but these observations were limited to one cruise in July of 1990 and were estimated indirectly based on stoichiometry of dissolved inorganic nitrogen and oxygen fluxes . Fennel et al. (2009) provided denitrification rates from the Louisiana shelf, measured by Gardner and McCarthy (unpublished) in October 2006, that averaged 2.0 (SE = 0.3) mmol N m−2 day−1.
There have been only a few other studies of denitrification on the continental shelf using the N2 flux method. The average denitrification rates obtained from the Arctic shelf (Devol et al. 1997), Massachusetts Bay (Nowicki et al. 1997), and the mid-Atlantic Bight (Laursen and Seitzinger 2002) ranged from 1.3 to 1.7 mmol N m−2 day−1, similar to the average rate of 1.4 (SE = 0.08) determined in this study. On the Washington shelf, higher denitrification rates (average = 3.2 mmol N m−2 day−1) were observed (Devol and Christensen 1993). More recently, denitrification measured from a depth range of 50–100 m in the Irish Sea region of the north Atlantic shelf averaged 0.l mmol N m−2 day−1 (Trimmer and Nicholls 2009), whereas rates measured in the Baltic Sea in the same depth range as for the sites reported herein ranged from 0.1 to 0.5 mmol N m−2 day−1 (Deutsch et al. 2010). The latter two studies both used the isotope-pairing method to estimate denitrification (Nielsen 1992). In a comparison of the isotope-pairing method with the N2:Ar flux method, the isotope-pairing method was found to significantly underestimate the denitrification rate observed directly with the N2 flux method (Ferguson and Eyre 2007). Ferguson and Eyre (2007) identify a need for more method comparison studies, across a range of environmental conditions. Such studies are needed to assess whether differences in denitrification rates are attributable to environmental differences or to methodological differences.
The N2 flux rates presented offer a means to evaluate the role of denitrification as a nitrogen sink. If the average denitrification rate of 1.4 mmol N m−2 day−1 is assumed to be representative of the shelf, a first order estimate of the denitrification sink may be calculated. Assuming the 200 meter bathymetry contour as the seaward boundary of the continental shelf (Seitzinger et al. 2006), the area of the Louisiana shelf is estimated to be 65,910 km2. Hence, shelf-wide denitrification would be on the order of 2.8 × 109 mol N per month. Given an estimated mean N load of 7.0 × 109 mol N per month delivered directly to the shelf from the Mississippi River for the months March to September (1968–2007), the denitrification sink would account for 39% (SE = 5%, estimated by error propagation) of this load. These results are comparable to estimates for other shelf systems where nitrogen sinks through denitrification have been estimated to account for 30–60% of nitrogen loads (Seitzinger et al. 2006; Deutsch et al. 2010).
NO3 − fluxes strongly covaried with O2 concentrations (Fig. 5) and were observed to have a complex, multi-variable relationship with bottom-water O2 concentration, NH4 + flux, and N2 flux (Fig. 6). The largest NO3 − flux out of the sediments (Table 2) occurred at Z04 during March 2005 where the bottom-water O2 concentration (141 mmol m−3) was among the highest observed. This large NO3 − flux coincided with the largest N2 flux and among the smallest NH4 + fluxes observed (Table 2). The largest NO3 − flux into the sediments occurred at Z02 in June 2006 and coincided with the lowest bottom-water O2 concentration (12 mmol m−3) and among the highest observed NO3 − concentrations (8.6 mmol m−3). A large NH4 + flux and a small N2 flux were also observed at this site in June 2006 and suggested that under these low O2 conditions coupled nitrification–denitrification was inhibited and that uptake of NO3 − may have been largely driven by dissimilatory nitrate reduction to ammonia or DNRA (McCarthy et al. 2008).
Because most sites were not O2 depleted in this study, denitrification appeared to proceed primarily through coupled nitrification–denitrification. Evidence for coupled nitrification–denitrification included the positive relationships between bottom-water O2 concentration and NO3 − flux (Fig. 5) and between NO3 − flux and N2 flux (r = 0.54, P = 0.02). The lack of an observed relationship between bottom-water NO3 − concentration and denitrification was consistent with previous observations indicating that coupled nitrification–denitrification was the dominant pathway (>90%) for denitrification when bottom-water NO3 − concentrations were less than 10 mmol m−3 (Seitzinger et al. 2006), as it was at most sites in this study (Table 1). Bottom-water O2 concentrations must be sufficient for coupled nitrification–denitrification to occur, and the results suggested that hypoxic bottom waters reduced coupled nitrification–denitrification and thereby increased NH4 + efflux from the sediment. Similar, hypoxia-induced patterns of sediment-water nitrogen fluxes have been reported from a number of coastal systems (reviewed by Middelburg and Levin 2009).
There was not a significant relationship between sediment O2 flux and N2 flux as has been observed in other analyses (Fennel et al. 2009). This may be due to the lower range of rates for O2 (Fig. 4) and N2 (Table 2) observed in this study as compared to the ranges of O2 and denitrification rates, 0 to −95 mmol O2 m−2 day−1 and 0 to 10 mmol N m−2 day−1, respectively, reported in Fig. 4 of Fennel et al. (2009). Further, the Fennel et al. (2009) dataset did not contain any sites with O2 concentrations less than 63 mmol m−3, whereas in the present dataset 33% of the stations had O2 concentrations that were hypoxic (ranging from 12 to 62 mmol m−3). The interactions observed between bottom-water O2 concentrations and flux rates (Figs. 5, 6) indicate that a linear paramterization of denitrification as a function of O2 flux may be too simplistic for this environment.
Other modeling exercises have determined that denitrification was sensitive to the organic matter sedimentation rate and bottom-water concentrations of O2 and nitrate (Middelburg et al. 1996). In the current study, it was not possible to estimate the sedimentation rate. Though, empirical relationships observed between water-column chla and both DIC and N2 flux rates (data not shown), indicated the potential coupling between labile organic matter in the water-column and sediment-water flux rates. Further, the lack of a relationship between sediment organic matter concentrations (Table 1) and any of the flux rates suggested that organic matter quality was more important than quantity. A similar conclusion was inferred for the sediments of the mid-Atlantic bight (Laursen and Seitzinger 2002). In sum, there is still much uncertainty about the controls of denitrification on this shelf, but O2 concentrations appear to be a primary determinant of nitrification/denitrification rates (Childs et al. 2002). Hence, hypoxia may act as a positive feeback to eutrophication by reducing nitrification/denitrification and, in turn, increasing the release of NH4 + from the sediments.
Zones of similarity
Empirical relationships between MR nutrient loads and hypoxic area explain 50–60% of the variability in mid-summer hypoxia area, but regression model predictions of the amount of nutrient reductions required to meet the management goal of an average hypoxic area of 5,000 km2 have high uncertainty (Greene et al. 2009). To reduce uncertainties in model predictions, representative hydrodynamic-eutrophication models are being developed. However, in the mechanistic sense there is high uncertainty about many of the physical transport and biogeochemical processes that are required to be modeled to accurately represent the development of hypoxia. This is especially true for sediment-water exchanges for which there have been few measurements. Identification of zones of similar sediment-water flux characteristics would reduce some of the modeling uncertainty associated with parameterizing initial and boundary conditions for the sediments.
The conceptual schemes proposed by Rowe and Chapman (2002) and Dale et al. (2010) included some hypothesized spatial patterns that may be evaluated with data collected from this study. Rowe and Chapman (2002) hypothesized that the sediment respiration in zone 1 would be primarily anaerobic owing to the high sedimentation rates from the plume and an abundance of oxidized iron and manganese that could be used as electron acceptors. They suggested aerobic and anaerobic metabolism would be balanced, i.e. O2 uptake would be equivalent to DIC release from the sediments, in zone 2 as long as O2 was present. In zone 3 it was hypothesized that O2 concentration in the bottom-water would be sufficient to maintain elevated rates of aerobic versus anaerobic respiration. In the present study, the DIC flux rates were generally greater than the O2 flux rates (Fig. 4). These results indicated that anaerobic processes were dominant in these sediments. Thus, realistic models of sediment O2 dynamics for this shelf will need to include the accumulation of oxygen debt from reduced nitrogen, iron, managanese, and sulfur.
Rowe and Chapman (2002) hypothesized that primary production rates would be highest in zone 2 owing to alleviation of light limitation and abundant nutrients in this region. However, results presented in Fig. 7 indicated that Z01 and Z04 had higher primary production than Z02 and Z03. Spatial patterns in euphotic zone chla concentrations mirrored the patterns observed in primary production with elevated values observed at Z01 and Z04 and lower values at Z02 and Z03.
Dale et al. (2010) hypothesized that zone 2 sub-pycnocline respiration would be dominated by water-column respiration while zone 3 sub-pycnocline respiration would be dominated by the sediments. Across the shelf, sediment metabolism was previously estimated to be the dominant respiratory pathway beneath the pycnocline, on average accounting for 75% of the respiration (Dortch et al. 1994; Quiñones-Rivera et al. 2007). However, a recent study based on sediment-water O2 flux measurements and water-column measurements of oxygen demand below the pycnocline found that sediments were responsible for 20 ± 4% of the sub-pycnocline respiration (Murrell and Lehrter 2011). In the present study it was observed that O2 fluxes were often small compared to DIC fluxes (Fig. 4). Recalculation of the sediment contribution to sub-pycnocline respiration using these DIC exchange rates yielded sediment respiration contributing 19–31% of the sub-pycnocline respiration (sediments + water-column, assumed a water-column respiration stoichiometry of DIC/O2 = 1). Some of the variation in the percent contributed by sediments could be attributed to differences in the thickness of the bottom-water layers (e.g. Fig. 3). At smaller sub-pynocline water depths, the relative contribution of the sediments was greater. The average thickness of the water-column below the pycnocline was 12, 9, 8, and 4 m at Z01, Z02, Z03, and Z04, respectively.
An updated conceptual model of the role of the sediments in system metabolism on the Louisiana continental shelf
The spatial patterns observed in salinity data (Fig. 1), sediment respiration (Fig. 4), and primary production (Fig. 7) indicated that the greatest variability in hydrography and metabolism on the shelf existed in an inshore to offshore, or north to south, gradient. Only in the lower Mississippi plume region did the salinity gradient orient itself in an east to west direction (Fig. 1). As such, we have redrawn the zones of similarity (Fig. 8). The new conceptual model has 4 zones, but the zonal scheme differs from Dale et al. (2010) in that the zones are expanded to the maximal extent of the hypoxic zone and the gradients are oriented in a north to south direction. In Fig. 8, the 5, 10, and 30 m bathymetry contours are used as features to delineate the zones. Based on how the stations from this study map against the zones in Fig. 8, we propose that zones 1 and 4 will have sediment-water flux and water-column production rates similar to sites Z01 and Z04, respectively. The new zone 2 encompasses site Z02 and Z03, based on similarities between these sites, and, thus, may be characterized by the results presented for these sites. The new zone 3 is envisioned as an oligotrophic region, similar to the original concept, but is displaced further to the south at depths > 30 m. None of the sites in the present study occupied the new zone 3. This refinement of the zonal model provides a new spatial scaling scheme for estimating the significance of sediment-water fluxes in the hypoxic region. Further measurements of sediment-water fluxes along inshore to offshore transects are needed to validate this zonal scheme.
In summary, three relationships were observed for the shelf that had previously been inferred. (1) Changes in bottom-water O2 concentrations strongly affected the magnitude and direction of nitrogen fluxes. This has important implications for feedbacks between increased nutrient loading, hypoxia, and the manner in which the shelf processes nutrients. (2) Observed N2 flux rates indicated that denitrification was a major nitrogen sink. (3) Sediment nutrient fluxes were generally small percentages of total water-column phytoplankton nutrient demand, though sediments could be important nutrient sources for sub-pycnocline primary production. These observations constrain the potential role of sediments in carbon, oxygen, and nutrient dynamics and will be useful in the development of mechanistic models for simulating shelf hypoxia.
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Acknowledgments
We thank A. Almario, J. Aukamp, M. Barron, J. Campbell, G. Craven, L. Oliver, R. Quarles, J. Scott, and R. Stanley for assistance in the collection and analysis of sediment samples. We thank J. Cornwell and M. Owens for analyzing N2, O2, and Ar samples from several of the early cruises. We thank the US EPA Office of Water and Gulf of Mexico Program Office for their support of ship time, and the crews of the OSV Bold and the R/V Longhorn which made this work possible. We thank C. Peacher, P. Caraballo, D. Bullock, and the facilties’ staff for designing and building the sediment incubation systems, and for all the other facilities support for this work. Critical reviews by R. Greene, W. Gardner, J. Melack and three anonymous reviewers greatly improved this manuscript. We dedicate this contribution to the memory of our friend and EPA colleague R.L. Quarles. This study was funded, reviewed, and approved for publication by the US EPA, National Health and Environmental Effects Research Laboratory; however, the contents are solely the views of the authors. Use of trade names or commercial products does not constitute endorsement by the US EPA. Contribution number 1373 from the US EPA, Gulf Ecology Division.
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Lehrter, J.C., Beddick, D.L., Devereux, R. et al. Sediment-water fluxes of dissolved inorganic carbon, O2, nutrients, and N2 from the hypoxic region of the Louisiana continental shelf. Biogeochemistry 109, 233–252 (2012). https://doi.org/10.1007/s10533-011-9623-x
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DOI: https://doi.org/10.1007/s10533-011-9623-x