Abstract
Reservoirs are intrinsically linked to the rivers that feed them, creating a river–reservoir continuum in which water and sediment inputs are a function of the surrounding watershed land use. We examined the spatial and temporal variability of sediment denitrification rates by sampling longitudinally along an agriculturally influenced river–reservoir continuum monthly for 13 months. Sediment denitrification rates ranged from 0 to 63 μg N2O g ash free dry mass of sediments (AFDM)−1 h−1 or 0–2.7 μg N2O g dry mass of sediments (DM)−1 h−1 at reservoir sites, vs. 0–12 μg N2O gAFDM−1 h−1 or 0–0.27 μg N2O gDM−1 h−1 at riverine sites. Temporally, highest denitrification activity traveled through the reservoir from upper reservoir sites to the dam, following the load of high nitrate (NO −3 -N) water associated with spring runoff. Annual mean sediment denitrification rates at different reservoir sites were consistently higher than at riverine sites, yet significant relationships among theses sites differed when denitrification rates were expressed per gDM vs. per gAFDM. There was a significant positive relationship between sediment denitrification rates and NO −3 -N concentration up to a threshold of 0.88 mg NO −3 -N l−1, above which it appeared NO −3 -N was no longer limiting. Denitrification assays were amended seasonally with NO −3 -N and an organic carbon source (glucose) to determine nutrient limitation of sediment denitrification. While organic carbon never limited sediment denitrification, all sites were significantly limited by NO −3 -N during fall and winter when ambient NO −3 -N was low.
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Abbreviations
- AFDM:
-
ash free dry mass
- ANOVA:
-
analysis of variance
- APHA:
-
American Public Health Association
- C:
-
carbon
- chl a:
-
chlorophyll a
- CV:
-
coefficient of variation
- DM:
-
dry mass
- DOC:
-
dissolved organic carbon
- Figure:
-
figure
- g:
-
gram
- He:
-
helium
- h:
-
hour
- km:
-
kilometers
- l:
-
liter
- LSMeans:
-
least-squared means
- LSV:
-
Lake Shelbyville, Illinois
- m:
-
meter
- mg:
-
milligram
- ml:
-
milliliter
- mM:
-
millimolar
- N:
-
nitrogen
- N + C:
-
nitrogen plus carbon
- N2:
-
di-nitrogen gas
- N2O:
-
nitrous oxide
- NH4+:
-
ammonium
- NO −3 :
-
nitrate
- O2:
-
oxygen
- SE:
-
standard error
- TDN:
-
total dissolved nitrogen
- TN:
-
total nitrogen
- USEPA:
-
United States Environmental Protection Agency
- USGS:
-
United Stated Geologic Survey
- μg:
-
microgram
References
I. Ahlgren F. Sörensson T. Waara K. Vrede (1994) ArticleTitleNitrogen budget in relation to microbial transformations in lakes Ambio 23 367–377
R.B. Alexander R.A. Smith G.E. Schwarz (2000) ArticleTitleEffect of stream channel size on the delivery of nitrogen to the Gulf of Mexico Nature 403 758–761 Occurrence Handle10.1038/35001562 Occurrence Handle10693802
InstitutionalAuthorName[APHA] American Public Health Association (1995) Standard Methods for the Examination of Water and Waste EditionNumber19 American Public Health Association Washington DC, USA
R.M. Baxter (1977) ArticleTitleEnvironmental effects on dams and impoundments Annu. Rev. Ecol. Syst. 8 255–283 Occurrence Handle10.1146/annurev.es.08.110177.001351
M.J. Bernot W.K. Dodds W.S. Gardner M.J. McCarthy D. Sobolev J.L. Tank (2003) ArticleTitleComparing denitrification estimates for a Texas estuary by using acetylene inhibition and membrane inlet mass spectrometry Appl. Environ. Microbiol. 69 5950–5956 Occurrence Handle10.1128/AEM.69.10.5950-5956.2003 Occurrence Handle14532049
S.R. Carpenter N.F. Caraco D.L. Correll R.W. Howarth A.N. Sharpley V.H. Smith (1998) ArticleTitleNonpoint pollution of surface waters with phosphorous and nitrogen Ecol. Appl. 8 559–568
T.M. Cole H.H. Hannan (1990) Dissolved oxygen dynamic B.L. Kimmel F.E. Payne K.W. Thorton (Eds) Reservoir Limnology: Ecological Perspectives John Wiley and Sons, Inc New York 71–107
M.B. David L.E. Gentry D.A. Kovacic K.M. Smith (1997) ArticleTitleNitrogen balance in and export from an agricultural watershed J. Environ. Qual. 26 1038–1048
M. David L.E. Gentry (2000) ArticleTitleAnthropogenic inputs of nitrogen and phosphorous and riverine export for Illinois, USA J. Environ. Qual. 29 494–508
S. Fleischer A. Gustafson A. Joelsson J. Pansar L. Stibe (1994) ArticleTitleNitrogen removal in created ponds Ambio 23 349–357
R. Garcia-Ruiz S.N. Pattinson B.A. Whitton (1998a) ArticleTitleDenitrification in river sediments: relationship between process rate and properties of water and sediment Freshwater Biol. 39 467–476 Occurrence Handle10.1046/j.1365-2427.1998.00295.x
R. Garcia-Ruiz S.N. Pattinson B.A. Whitton (1998b) ArticleTitleDenitrification and nitrous oxide production in sediments of the Wiskea lowland eutrophic river Sci. Tot. Environ. 210/211 307–320 Occurrence Handle10.1016/S0048-9697(98)00020-5
J.E. Garvey E.A. Marschall R.A. Wright (1998) ArticleTitleFrom star charts to stoneflies: detecting relationships in continuous bivariate data Ecology 79 442–447
L.E. Gentry M.B. David K.M. Smith-Starks D.A. Kovacic (2000) ArticleTitleNitrogen fertilizer and herbicide transport from tile drained fields J. Environ. Qual. 29 232–240
D.A. Goolsby W.A. Battaglin B.T. Aulenbach R.P. Hooper (2001) ArticleTitleNitrogen input to the Gulf of Mexico J. Environ. Qual. 30 329–336 Occurrence Handle11285892
L.O. Hedin J.C. Fischer Particlevon N.E. Ostrom B.P. Kennedy M.G. Brown G.P. Robertson (1998) ArticleTitleThermodynamic constraints on nitrogen transformations and other biogeochemical processes at soil-stream interfaces Ecology 79 684–703
R.M. Holmes J.B. Jones SuffixJr. S.G. Fisher N.B. Grimm (1996) ArticleTitleDenitrification in a nitrogen-limited stream ecosystem Biogeochemistry 33 125–146 Occurrence Handle10.1007/BF02181035
Illinois Department of Agriculture. 2003. Land Cover of Illinois, Available from http://www.agr.state.il.us/gis/landcover.html (verified 18 Aug. 2003).
S.E. Inwood J.L. Tank M.J. Bernot (2005) ArticleTitlePatterns of denitrification associated with land use in 9 midwestern headwater streams J. N. Am. Benthol. Soc. 24 227–245 Occurrence Handle10.1899/04-032.1
K.S. Jørgensen (1989) ArticleTitleAnnual pattern of denitrification and nitrate ammonification in estuarine sediment Appl. Environ. Microbiol. 55 1841–1847
G. Jossette B. Leporcq N. Sanchez Philippon (1999) ArticleTitleBiogeochemical mass-balances (C, NP, Si) in three large reservoirs of the Seine Basin (France) Biogeochemistry 47 119–146 Occurrence Handle10.1007/BF00994919
H.F. Kaspar (1985) ArticleTitleThe denitrification capacity of sediment from a hypereutrophic lake Freshwater Biol. 15 449–453
V.J. Kelly (2001) ArticleTitleInfluences of reservoirs on solute transport: a regional-scale approach Hydrol. Processes 15 1227–1249 Occurrence Handle10.1002/hyp.211
R.H. Kennedy K.W. Thorton D.E. Ford (1985) Characterization of the reservoir ecosystem D. Gunnisson (Eds) Microbial Processes in Reservoirs Dr. W. Junk Publishers DordrechtThe Netherlands 25–38
R.H. Kennedy W.W. Walker (1990) Reservoir nutrient dynamics B.L. Kimmel F.E. Payne K.W. Thorton (Eds) Reservoir Limnology: Ecological Perspectives John Wiley and Sons, Inc. New York 109–131
B.L. Kimmel O.T. Lind L.J. Paulson (1990) Reservoir primary production B.L. Kimmel F.E. Payne K.W. Thorton (Eds) Reservoir Limnology: Ecological Perspectives John Wiley and Sons Inc. New York 133–191
I. Koike A. Hattori (1978) ArticleTitleDenitrification and ammonia formation in anaerobic coastal sediments Appl. Environ. Microbiol. 35 278–282
L.A. Martin P.J. Mulholland J.R. Webster H.M. Valett (2001) ArticleTitleDenitrification potential in sediments of headwater streams in the southern Appalachian Mountains, USA J. N. Am. Benthol. Soc. 20 505–519
P.B. McMahon K.F. Dennehy (1999) ArticleTitleN2O emissions from a nitrogen-enriched river Environ. Sci. Technol. 33 21–25 Occurrence Handle10.1021/es980645n
A.M. Mitchell D.S. Baldwin (1999) ArticleTitleThe effects of sediment desiccation on the potential for nitrification, denitrification, and methanogenesis in an Australian reservoir Hydrobiologia 392 3–11 Occurrence Handle10.1023/A:1003589805914
R.E. Murray R. Knowles (1999) ArticleTitleChloramphenicol inhibition of denitrifying enzyme activity in two agricultural soils Appl. Environ. Microbiol. 65 3487–3492 Occurrence Handle10427039
S.N. Pattinson R. Garcia-Ruiz B.A. Whitton (1998) ArticleTitleSpatial and seasonal variation in denitrification in the Swale-Ouse systema river continuum Sci. Tot. Environ. 210/211 289–305 Occurrence Handle10.1016/S0048-9697(98)00019-9
N.N. Rabalais R.E. Turner W.J. Wiseman SuffixJr. (2002) ArticleTitleGulf of Mexico hypoxiaa.k.a. “the dead zone” Annu. Rev. Ecol. Syst. 33 235–263 Occurrence Handle10.1146/annurev.ecolsys.33.010802.150513
W.B. Richardson E.A. Strauss L.A. Bartsch E.M. Monroe J.C. Cavanaugh L. Vingum D.M. Soballe (2004) ArticleTitleDenitrification in the upper Mississippi River: rates, controls, and contribution to nitrate flux Can. J. Fish. Aquat. Sci. 61 1102–1112 Occurrence Handle10.1139/f04-062
T.V. Royer J.L. Tank M.B. David (2004) ArticleTitleTransport and fate of nitrate in headwater agricultural streams in Illinois J. Environ. Qual. 33 1296–1304 Occurrence Handle15254111
J. Rudolph P. Frenzel N. Pfennig (1991) ArticleTitleAcetylene inhibition technique underestimates in situ denitrification rates in intact cores of freshwater sediments FEMS Microbiol. Ecol. 85 101–106 Occurrence Handle10.1016/0378-1097(91)90505-5
D.P. Sartory J.U. Grobbelaar (1984) ArticleTitleExtraction of chlorophyll a from freshwater plankton for spectrophotometric analysis Hydrobiologia 114 177–187
InstitutionalAuthorNameSAS Institute (1991) SAS/STAT User’s Guide6.04 SAS Institute Cary, North Carolina
D.L. Saunders J. Kalff (2001) ArticleTitleNitrogen retention in wetlands, lakes and rivers Hydrobiologia 443 205–212 Occurrence Handle10.1023/A:1017506914063
J.L. Schaller T.V. Royer M.B. David J.L. Tank (2004) ArticleTitleDenitrification associated with plants and sediments in an agricultural stream J. N. Am. Benthol. Soc. 23 667–676 Occurrence Handle10.1899/0887-3593(2004)023<0667:DAWPAS>2.0.CO;2
Schlesinger W.H.(ed.) 1997. The global cycles of nitrogen and phosphorous. In: Biogeochemistry:An Analysis of Global Change. 2nd ed. Academic Press, San Diego, California 92101, USA, pp. 383–400.
S.P. Seitzinger (1988) ArticleTitleDenitrification in freshwater and coastal marine ecosystems: ecological and geochemical significance Limnol. Oceanogr. 33 IssueID4 part 2 702–724
S.P. Seitzinger L.P. Nielsen J. Caffrey P.B. Christensen (1993) ArticleTitleDenitrification measurements in aquatic sediments: a comparison of three methods Biogeochemistry 23 147–167 Occurrence Handle10.1007/BF00023750
S.P. Seitzinger R.V. Styles E.W. Boyer R.B. Alexander G. Billen R.W. Howarth B. Mayer N. Breeman ParticleVan (2002) ArticleTitleNitrogen retention in rivers: model development and application to watersheds in the northeastern U.S.A Biogeochemistry 57 IssueID58 199–237 Occurrence Handle10.1023/A:1015745629794
M.S. Smith J.M. Tiedje (1979) ArticleTitlePhases of denitrification following oxygen depletion in soil Soil Biol. Biochem. 11 261–267 Occurrence Handle10.1016/0038-0717(79)90071-3
L. Solorzano (1969) ArticleTitleDetermination of ammonia in natural water by the phenolhypochlorite method Limnol. Oceanogr. 14 799–801
G. Stanford J.O. Legg S. Dzienia E.C. Simpson SuffixJr. (1975) ArticleTitleDenitrification and associated nitrogen transformations in soils Soil Sci. 120 147–152
J.L. Tank W.K. Dodds (2003) ArticleTitleNutrient limitation of epilithic and epixylic biofilms in ten North American streams Freshwater Biol. 48 1031–1049 Occurrence Handle10.1046/j.1365-2427.2003.01067.x
Thorton K.W., Kennedy R.H., Carroll J.H., Walker W.W., Gunkel R.C. and Ashby S. 1980. Reservoir sedimentation and water quality – a heuristic model. In: Proceedings of the Symposium on Surface Water Impoundments. June 2–5, Minneapolis, MN, pp. 654–664.
J. Tiedje (1982) Denitrification A.L. Page (Eds) Methods of Soil Analysis Part 2 Chemical and Microbiological Properties ASA-SSSA Madison WI, USA 1011–1026
D.W. Toetz (1973) Nitrogen budget of the Great Plains impoundments W.C. Ackerman G.F. White E.B. Worthington (Eds) Man Made Lakes: Their Problems and Environmental Effects American Geophysical Union Washington DC 567–571
A.R. Townsend R.W. Howarth F.A. Bazzaz M.S. Booth C.C. Cleveland S.K. Collinge A.P. Dobson P.R. Epstein E.A. Holland D.R. Keeney M.A. Mallin C.A. Rogers P. Wayne A.H. Wolfe (2003) ArticleTitleHuman health effects of a changing global nitrogen cycle Front. Ecol. Environ. 1 IssueID5 240–246
[USEPA] United Stated Environmental Protection Agency. 1993. Method 300.0, Test Method for the Determination of Inorganic Anions in Water by Ion Chromatography. Environmental Monitoring Systems Laboratory, ORD, USEPA, Cincinnati, Ohio 45268.
[USEPA] United Stated Environmental Protection Agency. 1997. Method 445.0 rev1.2, In vitro Determination of Chlorophyll a and Pheophytin a in Marine and Freshwater Algae by Fluorescence. National Exposure Research Laboratory, ORD, USEPA, Cincinnati, Ohio 45268.
[USGS] United States Geological Survey. 2003. Daily Stream Flow Data [Online]. Available from http://www-il.usgs.gov/nwis-w/IL (verified 18 June 2003).
P.M. Vitousek J.D. Aber R.W. Howarth G.E. Likens P.A. Matson D.W. Schindler W.H. Schlesinger D.G. Tilman (1997) ArticleTitleHuman alteration of the global nitrogen cycle: sources and consequences Ecol. Appl. 7 737–750
Wetzel R.G. (ed.). 2002. Rivers and Lakes-their distribution, origins and forms. In: Limnology:Lake and River Ecosystems. 3rd ed. Academic Press, USA, pp 37–38.
Y. Xue D.A. Kovacic M.B. David L.E. Gentry R.L. Mulvaney C.W. Lindau (1999) ArticleTitle In situ measurements of denitrification in constructed wetlands J. Environ. Qual. 28 263–269
T. Yoshinari R. Knowles (1976) ArticleTitleAcetylene inhibition of nitrous-oxide reduction by denitrifying bacteria Biochem. Biophys. Res. Comm. 69 705–710 Occurrence Handle10.1016/0006-291X(76)90932-3 Occurrence Handle817722
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Wall, L.G., Tank, J.L., Royer, T.V. et al. Spatial and Temporal Variability in Sediment Denitrification Within an Agriculturally Influenced Reservoir. Biogeochemistry 76, 85–111 (2005). https://doi.org/10.1007/s10533-005-2199-6
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DOI: https://doi.org/10.1007/s10533-005-2199-6