Abstract
Long-term, weekly measurements of streamwater nitrogen and phosphorus concentrations in the West Fork of Walker Branch, a 1st order forested stream in eastern Tennessee, were used to assess the importance of in-stream processes for controlling stream concentrations and watershed exports. Over the period from 1991 to 2002, there was a slight declining trend in watershed export of dissolved inorganic N via streamflow, despite relatively high and constant wet N deposition rates (5 kg/ha/y). The watershed retains >90% of N deposition inputs. Concentrations of NO3− and soluble reactive phosphorus (SRP) showed distinct seasonal patterns with autumn and early spring minima and summer maxima. An end-member mixing analysis indicated that these seasonal concentration patterns were largely a result of seasonal variations in in-stream uptake processes, with net uptake of NO3− and SRP having the greatest impact on streamwater concentrations in November (reductions of 29 μg N/l and 2.5 μg P/l, respectively). This was likely a result of high rates of uptake by microbes colonizing new inputs of leaf detritus. For NO3− there was a secondary peak in net uptake in March and April (about 9 μg N/l) resulting from increased uptake by stream algae and bryophytes. Summer was a period of net release of NO3− to streamwater (peaking at 9 μg N/l in July) and minimal net effects on SRP concentrations. On average, in-stream processes resulted in removal of about 20% of the NO3− and 30% of the SRP entering the stream from the catchment annually. This study, as well as other recent work, suggests that in-stream processes are important buffers on stream nutrient concentrations and exports reducing the effects of changes in inputs and retention in terrestrial portions of watersheds.
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References
J.D. Aber C.L. Goodale S.V. Ollinger M. Smith A.H. Magill M.E. Martin R.A. Hallett J.L. Stoddard (2003) ArticleTitleIs nitrogen deposition altering the nitrogen status of northeastern forests? BioScience 53 403–426
J.D. Aber J.M. Melillo K.J. Nadelhoffer J. Pastor R.D. Boone (1991) ArticleTitleFactors controlling nitrogen cycling and nitrogen saturation in northern forest ecosystems Ecol. Appl. 1 305–315
J.D. Aber S.V. Ollinger C.T. Driscoll G.E. Likens R.T. Holmes R.J. Freuder C.L. Goodale (2002) ArticleTitleInorganic nitrogen losses from a forested ecosystem in response to physical, chemical, biotic, and climatic perturbations Ecosystems 5 648–658
R.B. Alexander R.A. Smith G.E. Schwarz (2000) ArticleTitleEffect of stream channel size on the delivery of nitrogen to the Gulf of Mexico Nature 403 758–761
American Public Health Association (APHA) 1992. Standard Methods for the Examination of Water and Waste Water. 18th ed. American Public Health Association, Washington, DC.
Böhlke J.K., Harvey J.W. and Voytek M.A. 2004. Reach-scale isotope tracer experiment to quantify denitrification in a nitrate-contaminated streammid-continent USA. Limnol. Oceanogr. 49: 821–838.
E.W. Boyer C.L. Goodale N.A. Jaworski R.W. Howarth (2002) ArticleTitleAnthropogenic nitrogen sources and relationships to riverine nitrogen export in the northeastern USA Biogeochemistry 57/58 137–169
D.A. Burns (1998) ArticleTitleRetention of NO3− in an upland stream environment: a mass balance approach Biogeochemistry 40 73–96
P.M. Chalk D.R. Keeney (1971) ArticleTitleNitrate and ammonium contents of Wisconsin limestones Nature 229 42
I.F. Creed L.E. Band (1998) ArticleTitleExport of nitrogen from catchments within a temperate forest: evidence for a unifying mechanism regulated by variable source area dynamics Water Resour. Res. 34 3105–3120
V.H. Dale L.K. Mann R.J. Olson D.W. Johnson K.C. Dearstone (1990) ArticleTitleThe long-term influence of past land use on the Walker Branch forest Landscape Ecol. 4 211–224
C.T. Driscoll K.M. Driscoll K.M. Roy M.J. Mitchell (2003) ArticleTitleChemical response of lakes in the Adirondack region of New York to declines in acidic deposition Environ. Sci. Technol. 37 2036–2042
K.N. Eshleman R.P. Morgan J.R. Webb F.A. Deviney J.N. Galloway (1998) ArticleTitleTemporal patterns of nitrogen leakage from mid-Appalachian forested watersheds: role of forest disturbance Water Resour. Res. 34 2005–2116
C.L. Goodale J.D. Aber W.H. McDowell (2000) ArticleTitleThe long-term effects of disturbance on organic and inorganic nitrogen export in the White Mountains, New Hampshire Ecosystems 3 433–450
C.L. Goodale J.D. Aber P.M. Vitousek (2003) ArticleTitleAn unexpected nitrate decline in New Hampshire streams Ecosystems 6 75–86
N.B. Grimm (1987) ArticleTitleNitrogen dynamics during succession in a desert stream Ecology 68 1157–1170
P.M. Groffman G. Howard A.J. Gold W.M. Nelson (1996) ArticleTitleMicrobial nitrate processing in shallow groundwater in a riparian forest J. Environ. Qual. 25 1309–1316
A.R. Hill (1983) Nitrate-N mass balances for two Ontario rivers T.D. Fontaine S.M. Bartell (Eds) Dynamics of Lotic Ecosystems Science Ann Arbor 457–477
A.R. Hill (1996) ArticleTitleNitrate removal in stream riparian zones J. Environ. Qual. 25 743–755
W.R. Hill P.J. Mulholland E.R. Marzolf (2001) ArticleTitleStream ecosystem responses to forest leaf emergence in spring Ecology 82 2306–2319
R.P. Hooper N. Christophersen N.E. Peters (1990) ArticleTitleModeling streamwater chemistry as a mixture of soil-water end members – an application to the Panola Mountain watershedGeorgiaUSA J. Hydrol. 116 32–43
R.W. Howarth G. Billen D. Swaney A. Townsend N. Jaworski K. Lajtha J.A. Downing R. Elmgren N. Caraco T. Jordan F. Berendse J. Freney V. Kudeyarov P. Murdoch Zhao-Liang Zhu (1996) ArticleTitleRegional nitrogen budgets and riverine N & P fluxes for the drainages to the North Atlantic Ocean: natural and human influences Biogeochemistry 35 75–139
D.W. Johnson (1985) Site description D.W. Johnson R.I. Van Hook (Eds) Analysis of Biogeochemical Cycling Processes in Walker Branch Watershed Springer-Verlag New York 6–20
G.P. Lewis G.E. Likens (2000) ArticleTitleLow stream nitrate concentrations associated with oak forests on the Allegheny High Plateau of Pennsylvania Water Resour. Res. 36 3091–3094
G.E. Likens F.H. Bormann R.S. Pierce J.S. Eaton N.M. Johnson (1977) Biogeochemistry of a Forested Ecosystem Springer-Verlag New York
G.M. Lovett K.C. Weathers W.V. Sobczak (2000) ArticleTitleNitrogen saturation and retention in forested watersheds of the Catskill Mountains, New York Ecol. Appl. 10 73–84
R.J. Luxmoore (1983) ArticleTitleWater budget of an eastern deciduous forest stand Soil Sci. Soc. Am. J. 47 785–791
M.E. McClain E.W. Boyer C.L. Dent S.E. GErgel N.B. Grimm P.M. Groffman S.C. Hart J.W. Harvey C.A. Johnston E. Mayorga W.H. McDowell G. Pinay (2003) ArticleTitleBiogeochemical hot spots and hot moments at the interface of terrestrial and aquatic ecosystems Ecosystems 6 301–312
W.C. McMaster (1963) Geologic map of the Oak Ridge Reservation, Tennessee. ORNL/TM-713 Oak Ridge National Laboratory Oak RidgeTennessee
J.L. Meyer G.E. Likens (1979) ArticleTitleTransport and transformation of phosphorus in a forest stream ecosystem Ecology 60 1255–1269
M.J. Mitchell C.T. Driscoll J.S. Khal G.E. Likens P.S. Murdoch L.H. Pardo (1996) ArticleTitleClimatic control of nitrate loss from forested watersheds in the northeast United States Environ. Sci. Technol. 30 2609–2612
P.J. Mulholland (1992) ArticleTitleRegulation of nutrient concentrations in a temperate forest stream: roles of uplandriparian, and in-stream processes Limnol. Oceanogr. 37 1512–1526
P.J. Mulholland (1993) ArticleTitleHydrometric and stream chemistry evidence of three storm flowpaths in Walker Branch Watershed J. Hydrol. 151 291–316
P.J. Mulholland J.W. Elwood J.D. Newbold J.R. Webster L.A. Ferren R.P. Perkins (1984) ArticleTitlePhosphorus uptake by decomposing leaf detritus: effect of microbial biomass and activity Verh. Int. Verein Limnol. 22 1899–1905
P.J. Mulholland W.R. Hill (1997) ArticleTitleSeasonal patterns in streamwater nutrient and dissolved organic carbon concentrations: separating catchment flow path and in-stream effects Water Resour. Res. 33 1297–1306
P.J. Mulholland E.R. Marzolf J.R. Webster D.R. Hart S.P. Hendricks (1997) ArticleTitleEvidence that hyporheic zones increase heterotrophic metabolism and phosphorus uptake in forest streams Limnol. Oceanogr. 42 443–451
P.J. Mulholland J.D. Newbold J.W. Elwood J.R. Webster (1985) ArticleTitlePhosphorus spiraling in a woodland stream: seasonal variations Ecology 66 1012–1023
P.J. Mulholland J.L. Tank D.M. Sanzone W.M. Wollheim B.J. Peterson J.R. Webster J.L. Meyer (2000) ArticleTitleNitrogen cycling in a forest stream determined by a 15N tracer addition Ecol. Monogr. 70 471–493
P.J. Mulholland G.V. Wilson P.M. Jardine (1990) ArticleTitleHydrogeochemical response of a forested watershed to storms: effects of preferential flow along shallow and deep pathways Water Resour. Res. 26 3021–3036
Mulholland P.J., Valett H.M., Webster J.R., Thomas S.A., Cooper L.N., Hamilton S.K. and Peterson B.J. 2004. Stream denitrification and total nitrate uptake rates measured using a field 15N isotope tracer approach. Limnol. Oceanogr. 49: 809–820.
P.S. Murdoch D.A. Burns G.B. Lawrence (1998) ArticleTitleRelation of climate change to the acidification of surface waters by nitrogen deposition Environ. Sci. Technol. 32 1642–1647
P.S. Murdoch J.L. Stoddard (1993) ArticleTitleChemical characteristics and temporal trends in eight streams of the Catskill Mountains, New York Water Air Soil Poll. 67 367–395
W.T. Peterjohn M.B. Adams F.S. Gilliam (1996) ArticleTitleSymptoms of nitrogen saturation in two central Appalachian hardwood forest ecosystems Biogeochemistry 35 507–522
B.J. Peterson W.M. Wollheim P.J. Mulholland J.R. Webster J.L. Meyer J.L. Tank E. Marti W.B. Bowden H.M. Valett A.E. Hershey W.H. McDowell W.K. Dodds S.K. Hamilton S. Gregory D.J. Morrall (2001) ArticleTitleControl of nitrogen export from watersheds by headwater streams Science 292 86–90
S.P. Seitzinger R.V. Styles E.W. Boyer R.B. Alexander G. Billen R.W. Howarth B. Mayer PrefixVan N. Breeman (2002) ArticleTitleNitrogen retention in rivers: model development and application to watersheds in the northeastern USA Biogeochemistry 57/58 199–237
A.L. Sjodin W.M. Lewis J.F. Saunders (1997) ArticleTitleDenitrification as a component of the nitrogen budget for a large plains river Biogeochemistry 39 327–342
Smith R.A., Alexander R.B. and Schwarz G.E. 2003. Estimating the natural background concentrations of nutrients in streams and rivers of the conterminous United States. Environ. Sci. Technol. 37: 3039–3047.
J.L. Stoddard (1994) Long-term changes in watershed retention of N L.A. Baker (Eds) Chemistry of Lakes and Reservoirs ACS Advances in Chemistry Series No. 237 American Chemical Society Washington, DC 223–284
W.T. Swank (1988) Stream chemistry responses to disturbance W.T. Swank D.A. Crossley SuffixJr. (Eds) Forest Ecology and Hydrology at Coweeta Springer-Verlag New York 339–358
W.T. Swank W.H. Caskey (1982) ArticleTitleNitrate depletion in a second-order mountain stream J. Environ. Qual. 11 581–584
W.T. Swank J.M. Vose (1997) ArticleTitleLong-term nitrogen dynamics of Coweeta forested watersheds in the southeastern United States of America Global Biogeochem. Cycles 11 657–671
J.L. Tank J.L. Meyer D.M. Sanzone P.J. Mulholland J.R. Webster B.J. Peterson W.M. Wollheim N.E. Leonard (2000) ArticleTitleAnalysis of nitrogen cycling in a forest stream during autumn using a 15N tracer addition Limnol. Oceanogr. 45 1013–1029
F.J. Triska B.M. Buckley (1978) ArticleTitlePatterns of nitrogen uptake and loss in relation to litter disappearance and associated invertebrate biomass in six streams of the Pacific NorthwestUSA Verh. Int. Verein. Limnol. 20 1324–1332
F.J. Triska V.C. Kennedy R.J. Avanzano G.W. Zellweger K.E. Bencala (1989) ArticleTitleRetention and transport of nutrients in a third-order stream in northwestern California: hyporheic processes Ecology 70 1893–1905
F.J. Triska J.R. Sedell K. Cromack S.V. Gregory F.M. McCorison (1984) ArticleTitleNitrogen budget for a small coniferous forest stream Ecol. Monogr. 54 119–140
H.M. Valett J.A. Morrice C.N. Dahm (1996) ArticleTitleParent lithology, surface-groundwater exchangeand nitrate retention in headwater streams Limnol. Oceanogr. 41 333–345
P.M. Vitousek J.D. Aber R.W. Howarth G.E. Likens P.A. Matson D.W. Schindler W.H. Schlesinger D.G. Tilman (1997) ArticleTitleHuman alteration of the global nitrogen cycle: sources and consequences Ecol. Appl. 7 737–750
G.V. Wilson P.M. Jardine R.J. Luxmoore L.W. Zelazny D.A. Lietzke D.E. Todd (1991) ArticleTitleHydrogeochemical processes controlling subsurface transport from an upper subcatchment of Walker Branch Watershed during storm events, 1, hydrological transport processes J. Hydrol. 123 297–316
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Mulholland, P. The importance of in-stream uptake for regulating stream concentrations and outputs of N and P from a forested watershed: evidence from long-term chemistry records for Walker Branch Watershed. Biogeochemistry 70, 403–426 (2004). https://doi.org/10.1007/s10533-004-0364-y
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DOI: https://doi.org/10.1007/s10533-004-0364-y